Induction of caveolin during adipogenesis and association of GLUT4 with caveolin-rich vesicles

doi:10.1083/jcb.127.5.1233

This Article

	Full Text (PDF, 2487K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Scherer, P. E.
	Articles by Lodish, H. F.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Scherer, P. E.
	Articles by Lodish, H. F.


Social Bookmarking

	What's this?

ARTICLES

Induction of caveolin during adipogenesis and association of GLUT4 with caveolin-rich vesicles

PE Scherer, MP Lisanti, G Baldini, M Sargiacomo, CC Mastick and HF Lodish
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142-1479.

Caveolae, also termed plasmalemmal vesicles, are small, flask-shaped, non-clathrin-coated invaginations of the plasma membrane. Caveolin is a principal component of the filaments that make up the striated coat of caveolae. Using caveolin as a marker protein for the organelle, we found that adipose tissue is the single most abundant source of caveolae identified thus far. Caveolin mRNA and protein are strongly induced during differentiation of 3T3-L1 fibroblasts to adipocytes; during adipogenesis there is also a dramatic increase in the complexity of the protein composition of caveolin-rich membrane domains. About 10- 15% of the insulin-responsive glucose transporter GLUT4 is found in this caveolin-rich fraction, and immuno-isolated vesicles containing GLUT4 also contain caveolin. However, in non-stimulated adipocytes the majority of caveolin fractionates with the plasma membrane, while most GLUT4 associates with low-density microsomes. Upon addition of insulin to 3T3-L1 adipocytes, there is a significant increase in the amount of GLUT4 associated with caveolin-rich membrane domains, an increase in the amount of caveolin associated with the plasma membrane, and a decrease in the amount of caveolin associated with low-density microsomes. Caveolin does not undergo a change in phosphorylation upon stimulation of 3T3-L1 adipocytes with insulin. However, after treatment with insulin it is associated with a 32-kD phosphorylated protein. Caveolae thus may play an important role in the vesicular transport of GLUT4 to or from the plasma membrane. 3T3-L1 adipocytes offer an attractive system to study the function of caveolae in several cellular trafficking and signaling events.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Gonzalez-Munoz, E., Lopez-Iglesias, C., Calvo, M., Palacin, M., Zorzano, A., Camps, M. (2009). Caveolin-1 Loss of Function Accelerates Glucose Transporter 4 and Insulin Receptor Degradation in 3T3-L1 Adipocytes. Endocrinology 150: 3493-3502 [Abstract] [Full Text]
Bastiani, M., Liu, L., Hill, M. M., Jedrychowski, M. P., Nixon, S. J., Lo, H. P., Abankwa, D., Luetterforst, R., Fernandez-Rojo, M., Breen, M. R., Gygi, S. P., Vinten, J., Walser, P. J., North, K. N., Hancock, J. F., Pilch, P. F., Parton, R. G. (2009). MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. JCB 185: 1259-1273 [Abstract] [Full Text]
Okada, S., Yamada, E., Saito, T., Ohshima, K., Hashimoto, K., Yamada, M., Uehara, Y., Tsuchiya, T., Shimizu, H., Tatei, K., Izumi, T., Yamauchi, K., Hisanaga, S.-i., Pessin, J. E., Mori, M. (2008). CDK5-dependent Phosphorylation of the Rho Family GTPase TC10{alpha} Regulates Insulin-stimulated GLUT4 Translocation. J. Biol. Chem. 283: 35455-35463 [Abstract] [Full Text]
Schwenk, R. W., Luiken, J. J.F.P., Bonen, A., Glatz, J. F.C. (2008). Regulation of sarcolemmal glucose and fatty acid transporters in cardiac disease. Cardiovasc Res 79: 249-258 [Abstract] [Full Text]
Langlois, S., Cowan, K. N., Shao, Q., Cowan, B. J., Laird, D. W. (2008). Caveolin-1 and -2 Interact with Connexin43 and Regulate Gap Junctional Intercellular Communication in Keratinocytes. Mol. Biol. Cell 19: 912-928 [Abstract] [Full Text]
Liu, L., Pilch, P. F. (2008). A Critical Role of Cavin (Polymerase I and Transcript Release Factor) in Caveolae Formation and Organization. J. Biol. Chem. 283: 4314-4322 [Abstract] [Full Text]
Williams, D., Pessin, J. E. (2008). Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes. JCB 180: 375-387 [Abstract] [Full Text]
Kampf, J. P., Parmley, D., Kleinfeld, A. M. (2007). Free fatty acid transport across adipocytes is mediated by an unknown membrane protein pump. Am. J. Physiol. Endocrinol. Metab. 293: E1207-E1214 [Abstract] [Full Text]
Pilch, P. F., Souto, R. P., Liu, L., Jedrychowski, M. P., Berg, E. A., Costello, C. E., Gygi, S. P. (2007). Cellular spelunking: exploring adipocyte caveolae. J. Lipid Res. 48: 2103-2111 [Abstract] [Full Text]
Predescu, S. A., Predescu, D. N., Malik, A. B. (2007). Molecular determinants of endothelial transcytosis and their role in endothelial permeability. Am. J. Physiol. Lung Cell. Mol. Physiol. 293: L823-L842 [Abstract] [Full Text]
Koneru, S., Penumathsa, S. V., Thirunavukkarasu, M., Samuel, S. M., Zhan, L., Han, Z., Maulik, G., Das, D. K., Maulik, N. (2007). Redox regulation of ischemic preconditioning is mediated by the differential activation of caveolins and their association with eNOS and GLUT-4. Am. J. Physiol. Heart Circ. Physiol. 292: H2060-H2072 [Abstract] [Full Text]
Trujillo, M. E., Scherer, P. E. (2006). Adipose Tissue-Derived Factors: Impact on Health and Disease. Endocr. Rev. 27: 762-778 [Abstract] [Full Text]
Dasari, A., Bartholomew, J. N., Volonte, D., Galbiati, F. (2006). Oxidative Stress Induces Premature Senescence by Stimulating Caveolin-1 Gene Transcription through p38 Mitogen-Activated Protein Kinase/Sp1-Mediated Activation of Two GC-Rich Promoter Elements.. Cancer Res. 66: 10805-10814 [Abstract] [Full Text]
Nevins, A. K., Thurmond, D. C. (2006). Caveolin-1 Functions as a Novel Cdc42 Guanine Nucleotide Dissociation Inhibitor in Pancreatic beta-Cells. J. Biol. Chem. 281: 18961-18972 [Abstract] [Full Text]
Inoue, M., Chiang, S.-H., Chang, L., Chen, X.-W., Saltiel, A. R. (2006). Compartmentalization of the Exocyst Complex in Lipid Rafts Controls Glut4 Vesicle Tethering. Mol. Biol. Cell 17: 2303-2311 [Abstract] [Full Text]
Hardin, C. D., Vallejo, J. (2006). Caveolins in vascular smooth muscle: Form organizing function. Cardiovasc Res 69: 808-815 [Abstract] [Full Text]
Scioscia, M., Gumaa, K., Kunjara, S., Paine, M. A., Selvaggi, L. E., Rodeck, C. H., Rademacher, T. W. (2006). Insulin Resistance in Human Preeclamptic Placenta Is Mediated by Serine Phosphorylation of Insulin Receptor Substrate-1 and -2. J. Clin. Endocrinol. Metab. 91: 709-717 [Abstract] [Full Text]
Kiens, B. (2006). Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance. Physiol. Rev. 86: 205-243 [Abstract] [Full Text]
Veluthakal, R., Chvyrkova, I., Tannous, M., McDonald, P., Amin, R., Hadden, T., Thurmond, D. C., Quon, M. J., Kowluru, A. (2005). Essential Role for Membrane Lipid Rafts in Interleukin-1{beta}-Induced Nitric Oxide Release From Insulin-Secreting Cells: Potential Regulation by Caveolin-1+. Diabetes 54: 2576-2585 [Abstract] [Full Text]
Ranalletta, M., Jiang, H., Li, J., Tsao, T.S., Stenbit, A. E., Yokoyama, M., Katz, E. B., Charron, M. J. (2005). Altered Hepatic and Muscle Substrate Utilization Provoked by GLUT4 Ablation. Diabetes 54: 935-943 [Abstract] [Full Text]
Cohen, A. W., Schubert, W., Brasaemle, D. L., Scherer, P. E., Lisanti, M. P. (2005). Caveolin-1 Expression Is Essential for Proper Nonshivering Thermogenesis in Brown Adipose Tissue. Diabetes 54: 679-686 [Abstract] [Full Text]
Lin, Y., Berg, A. H., Iyengar, P., Lam, T. K. T., Giacca, A., Combs, T. P., Rajala, M. W., Du, X., Rollman, B., Li, W., Hawkins, M., Barzilai, N., Rhodes, C. J., Fantus, I. G., Brownlee, M., Scherer, P. E. (2005). The Hyperglycemia-induced Inflammatory Response in Adipocytes: THE ROLE OF REACTIVE OXYGEN SPECIES. J. Biol. Chem. 280: 4617-4626 [Abstract] [Full Text]
Berg, A. H., Lin, Y., Lisanti, M. P., Scherer, P. E. (2004). Adipocyte differentiation induces dynamic changes in NF-{kappa}B expression and activity. Am. J. Physiol. Endocrinol. Metab. 287: E1178-E1188 [Abstract] [Full Text]
Liu, L., Abramowitz, J., Askari, A., Allen, J. C. (2004). Role of caveolae in ouabain-induced proliferation of cultured vascular smooth muscle cells of the synthetic phenotype. Am. J. Physiol. Heart Circ. Physiol. 287: H2173-H2182 [Abstract] [Full Text]
Cohen, A. W., Hnasko, R., Schubert, W., Lisanti, M. P. (2004). Role of Caveolae and Caveolins in Health and Disease. Physiol. Rev. 84: 1341-1379 [Abstract] [Full Text]
Kanzaki, M., Furukawa, M., Raab, W., Pessin, J. E. (2004). Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling. J. Biol. Chem. 279: 30622-30633 [Abstract] [Full Text]
Kogo, H., Aiba, T., Fujimoto, T. (2004). Cell Type-specific Occurrence of Caveolin-1{alpha} and -1{beta} in the Lung Caused by Expression of Distinct mRNAs. J. Biol. Chem. 279: 25574-25581 [Abstract] [Full Text]
Cohen, A. W., Razani, B., Schubert, W., Williams, T. M., Wang, X. B., Iyengar, P., Brasaemle, D. L., Scherer, P. E., Lisanti, M. P. (2004). Role of Caveolin-1 in the Modulation of Lipolysis and Lipid Droplet Formation. Diabetes 53: 1261-1270 [Abstract] [Full Text]
Watson, R. T., Kanzaki, M., Pessin, J. E. (2004). Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes. Endocr. Rev. 25: 177-204 [Abstract] [Full Text]
Kumar, A., Xiao, Y.-P., Laipis, P. J., Fletcher, B. S., Frost, S. C. (2004). Glucose deprivation enhances targeting of GLUT1 to lipid rafts in 3T3-L1 adipocytes. Am. J. Physiol. Endocrinol. Metab. 286: E568-E576 [Abstract] [Full Text]
Pajvani, U. B., Hawkins, M., Combs, T. P., Rajala, M. W., Doebber, T., Berger, J. P., Wagner, J. A., Wu, M., Knopps, A., Xiang, A. H., Utzschneider, K. M., Kahn, S. E., Olefsky, J. M., Buchanan, T. A., Scherer, P. E. (2004). Complex Distribution, Not Absolute Amount of Adiponectin, Correlates with Thiazolidinedione-mediated Improvement in Insulin Sensitivity. J. Biol. Chem. 279: 12152-12162 [Abstract] [Full Text]
Zhang, H., Links, P. H., Ngsee, J. K., Tran, K., Cui, Z., Ko, K. W. S., Yao, Z. (2004). Localization of Low Density Lipoprotein Receptor-related Protein 1 to Caveolae in 3T3-L1 Adipocytes in Response to Insulin Treatment. J. Biol. Chem. 279: 2221-2230 [Abstract] [Full Text]
Gaudreault, S. B., Chabot, C., Gratton, J.-P., Poirier, J. (2004). The Caveolin Scaffolding Domain Modifies 2-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Binding Properties by Inhibiting Phospholipase A2 Activity. J. Biol. Chem. 279: 356-362 [Abstract] [Full Text]
Pascariu, M., Bendayan, M., Ghitescu, L. (2004). Correlated Endothelial Caveolin Overexpression and Increased Transcytosis in Experimental Diabetes. J. Histochem. Cytochem. 52: 65-76 [Abstract] [Full Text]
Bae, S. S., Cho, H., Mu, J., Birnbaum, M. J. (2003). Isoform-specific Regulation of Insulin-dependent Glucose Uptake by Akt/Protein Kinase B. J. Biol. Chem. 278: 49530-49536 [Abstract] [Full Text]
Hnasko, R., Lisanti, M. P. (2003). The Biology of Caveolae: Lessons from Caveolin Knockout Mice and Implications for Human Disease. Mol. Interv. 3: 445-464 [Abstract] [Full Text]
Cohen, A. W., Combs, T. P., Scherer, P. E., Lisanti, M. P. (2003). Role of caveolin and caveolae in insulin signaling and diabetes. Am. J. Physiol. Endocrinol. Metab. 285: E1151-E1160 [Abstract] [Full Text]
Sotgia, F., Bonuccelli, G., Minetti, C., Woodman, S. E., Capozza, F., Kemp, R. G., Scherer, P. E., Lisanti, M. P. (2003). Phosphofructokinase Muscle-Specific Isoform Requires Caveolin-3 Expression for Plasma Membrane Recruitment and Caveolar Targeting: Implications for the Pathogenesis of Caveolin-Related Muscle Diseases. Am. J. Pathol. 163: 2619-2634 [Abstract] [Full Text]
Zeng, Y., Tao, N., Chung, K.-N., Heuser, J. E., Lublin, D. M. (2003). Endocytosis of Oxidized Low Density Lipoprotein through Scavenger Receptor CD36 Utilizes a Lipid Raft Pathway That Does Not Require Caveolin-1. J. Biol. Chem. 278: 45931-45936 [Abstract] [Full Text]
Evans, W. E. IV, Coyer, R. L., Sandusky, M. F., Van Fleet, M. J., Moore, J. G., Nyquist, S. E. (2003). Characterization of Membrane Rafts Isolated From Rat Sertoli Cell Cultures: Caveolin and Flotillin-1 Content. J Androl 24: 812-821 [Abstract] [Full Text]
Aravamudan, B., Volonte, D., Ramani, R., Gursoy, E., Lisanti, M. P., London, B., Galbiati, F. (2003). Transgenic overexpression of caveolin-3 in the heart induces a cardiomyopathic phenotype. Hum Mol Genet 12: 2777-2788 [Abstract] [Full Text]
Sotgia, F., Woodman, S. E., Bonuccelli, G., Capozza, F., Minetti, C., Scherer, P. E., Lisanti, M. P. (2003). Phenotypic behavior of caveolin-3 R26Q, a mutant associated with hyperCKemia, distal myopathy, and rippling muscle disease. Am. J. Physiol. Cell Physiol. 285: C1150-C1160 [Abstract] [Full Text]
Volonte, D., Peoples, A. J., Galbiati, F. (2003). Modulation of Myoblast Fusion by Caveolin-3 in Dystrophic Skeletal Muscle Cells: Implications for Duchenne Muscular Dystrophy and Limb-Girdle Muscular Dystrophy-1C. Mol. Biol. Cell 14: 4075-4088 [Abstract] [Full Text]
Mokelke, E. A., Hu, Q., Song, M., Toro, L., Reddy, H. K., Sturek, M. (2003). Altered functional coupling of coronary K+ channels in diabetic dyslipidemic pigs is prevented by exercise. J. Appl. Physiol. 95: 1179-1193 [Abstract] [Full Text]
Rubin, D., Ismail-Beigi, F. (2003). Distribution of Glut1 in detergent-resistant membranes (DRMs) and non-DRM domains: effect of treatment with azide. Am. J. Physiol. Cell Physiol. 285: C377-C383 [Abstract] [Full Text]
Liu, L., Mohammadi, K., Aynafshar, B., Wang, H., Li, D., Liu, J., Ivanov, A. V., Xie, Z., Askari, A. (2003). Role of caveolae in signal-transducing function of cardiac Na+/K+-ATPase. Am. J. Physiol. Cell Physiol. 284: C1550-C1560 [Abstract] [Full Text]
Souto, R. P., Vallega, G., Wharton, J., Vinten, J., Tranum-Jensen, J., Pilch, P. F. (2003). Immunopurification and Characterization of Rat Adipocyte Caveolae Suggest Their Dissociation from Insulin Signaling. J. Biol. Chem. 278: 18321-18329 [Abstract] [Full Text]
Shigematsu, S., Watson, R. T., Khan, A. H., Pessin, J. E. (2003). The Adipocyte Plasma Membrane Caveolin Functional/Structural Organization Is Necessary for the Efficient Endocytosis of GLUT4. J. Biol. Chem. 278: 10683-10690 [Abstract] [Full Text]
Pajvani, U. B., Du, X., Combs, T. P., Berg, A. H., Rajala, M. W., Schulthess, T., Engel, J., Brownlee, M., Scherer, P. E. (2003). Structure-Function Studies of the Adipocyte-secreted Hormone Acrp30/Adiponectin. IMPLICATIONS FOR METABOLIC REGULATION AND BIOACTIVITY. J. Biol. Chem. 278: 9073-9085 [Abstract] [Full Text]
Lee, H., Park, D. S., Razani, B., Russell, R. G., Pestell, R. G., Lisanti, M. P. (2002). Caveolin-1 Mutations (P132L and Null) and the Pathogenesis of Breast Cancer : Caveolin-1 (P132L) Behaves in a Dominant-Negative Manner and Caveolin-1 (-/-) Null Mice Show Mammary Epithelial Cell Hyperplasia. Am. J. Pathol. 161: 1357-1369 [Abstract] [Full Text]
Lee, H., Park, D. S., Wang, X. B., Scherer, P. E., Schwartz, P. E., Lisanti, M. P. (2002). Src-induced Phosphorylation of Caveolin-2 on Tyrosine 19. PHOSPHO-CAVEOLIN-2 (TYR(P)19) IS LOCALIZED NEAR FOCAL ADHESIONS, REMAINS ASSOCIATED WITH LIPID RAFTS/CAVEOLAE, BUT NO LONGER FORMS A HIGH MOLECULAR MASS HETERO-OLIGOMER WITH CAVEOLIN-1. J. Biol. Chem. 277: 34556-34567 [Abstract] [Full Text]
Razani, B., Woodman, S. E., Lisanti, M. P. (2002). Caveolae: From Cell Biology to Animal Physiology. Pharmacol. Rev. 54: 431-467 [Abstract] [Full Text]
Kimura, A., Mora, S., Shigematsu, S., Pessin, J. E., Saltiel, A. R. (2002). The Insulin Receptor Catalyzes the Tyrosine Phosphorylation of Caveolin-1. J. Biol. Chem. 277: 30153-30158 [Abstract] [Full Text]
Kanzaki, M., Pessin, J. E. (2002). Caveolin-associated Filamentous Actin (Cav-actin) Defines a Novel F-actin Structure in Adipocytes. J. Biol. Chem. 277: 25867-25869 [Abstract] [Full Text]
Yang, T. T. C., Xiong, Q., Enslen, H., Davis, R. J., Chow, C.-W. (2002). Phosphorylation of NFATc4 by p38 Mitogen-Activated Protein Kinases. Mol. Cell. Biol. 22: 3892-3904 [Abstract] [Full Text]
Razani, B., Wang, X. B., Engelman, J. A., Battista, M., Lagaud, G., Zhang, X. L., Kneitz, B., Hou, H. Jr., Christ, G. J., Edelmann, W., Lisanti, M. P. (2002). Caveolin-2-Deficient Mice Show Evidence of Severe Pulmonary Dysfunction without Disruption of Caveolae. Mol. Cell. Biol. 22: 2329-2344 [Abstract] [Full Text]
Garcia Dos Santos, E., Dieudonne, M. N., Pecquery, R., Le Moal, V., Giudicelli, Y., Lacasa, D. (2002). Rapid Nongenomic E2 Effects on p42/p44 MAPK, Activator Protein-1, and cAMP Response Element Binding Protein in Rat White Adipocytes. Endocrinology 143: 930-940 [Abstract] [Full Text]
Bickel, P. E. (2002). Lipid rafts and insulin signaling. Am. J. Physiol. Endocrinol. Metab. 282: E1-E10 [Abstract] [Full Text]
Lin, Y., Rajala, M. W., Berger, J. P., Moller, D. E., Barzilai, N., Scherer, P. E. (2001). Hyperglycemia-induced Production of Acute Phase Reactants in Adipose Tissue. J. Biol. Chem. 276: 42077-42083 [Abstract] [Full Text]
James, D. J., Cairns, F., Salt, I. P., Murphy, G. J., Dominiczak, A. F., Connell, J. M.C., Gould, G. W. (2001). Skeletal Muscle of Stroke-Prone Spontaneously Hypertensive Rats Exhibits Reduced Insulin-Stimulated Glucose Transport and Elevated Levels of Caveolin and Flotillin. Diabetes 50: 2148-2156 [Abstract] [Full Text]
Galbiati, F., Volonte', D., Liu, J., Capozza, F., Frank, P. G., Zhu, L., Pestell, R. G., Lisanti, M. P. (2001). Caveolin-1 Expression Negatively Regulates Cell Cycle Progression by Inducing G0/G1 Arrest via a p53/p21WAF1/Cip1-dependent Mechanism. Mol. Biol. Cell 12: 2229-2244 [Abstract] [Full Text]
Kimura, A., Baumann, C. A., Chiang, S.-H., Saltiel, A. R. (2001). The sorbin homology domain: A motif for the targeting of proteins to lipid rafts. Proc. Natl. Acad. Sci. USA 98: 9098-9103 [Abstract] [Full Text]
Muller, G., Jung, C., Wied, S., Welte, S., Jordan, H., Frick, W. (2001). Redistribution of Glycolipid Raft Domain Components Induces Insulin-Mimetic Signaling in Rat Adipocytes. Mol. Cell. Biol. 21: 4553-4567 [Abstract] [Full Text]
Bogan, J. S., McKee, A. E., Lodish, H. F. (2001). Insulin-Responsive Compartments Containing GLUT4 in 3T3-L1 and CHO Cells: Regulation by Amino Acid Concentrations. Mol. Cell. Biol. 21: 4785-4806 [Abstract] [Full Text]
Ginés, S., Ciruela, F., Burgueño, J., Casadó, V., Canela, Enric. I., Mallol, J., Lluís, C., Franco, R. (2001). Involvement of Caveolin in Ligand-Induced Recruitment and Internalization of A1 Adenosine Receptor and Adenosine Deaminase in an Epithelial Cell Line. Mol. Pharmacol. 59: 1314-1323 [Abstract] [Full Text]
Malide, D., Yewdell, J. W., Bennink, J. R., Cushman, S. W. (2001). The Export of Major Histocompatibility Complex Class I Molecules from the Endoplasmic Reticulum of Rat Brown Adipose Cells Is Acutely Stimulated by Insulin. Mol. Biol. Cell 12: 101-114 [Abstract] [Full Text]
Engelman, J. A., Berg, A. H., Lewis, R. Y., Lisanti, M. P., Scherer, P. E. (2000). Tumor Necrosis Factor {alpha}-Mediated Insulin Resistance, but Not Dedifferentiation, Is Abrogated by MEK1/2 Inhibitors in 3T3-L1 Adipocytes. Mol. Endocrinol. 14: 1557-1569 [Abstract] [Full Text]
Galbiati, F., Volonté, D., Chu, J. B., Li, M., Fine, S. W., Fu, M., Bermudez, J., Pedemonte, M., Weidenheim, K. M., Pestell, R. G., Minetti, C., Lisanti, M. P. (2000). Transgenic overexpression of caveolin-3 in skeletal muscle fibers induces a Duchenne-like muscular dystrophy phenotype. Proc. Natl. Acad. Sci. USA 10.1073/pnas.160249097v1 [Abstract] [Full Text]
Lloyd, P. G., Hardin, C. D. (2000). Sorting of metabolic pathway flux by the plasma membrane in cerebrovascular smooth muscle cells. Am. J. Physiol. Cell Physiol. 278: C803-C811 [Abstract] [Full Text]
Engelman, J. A., Berg, A. H., Lewis, R. Y., Lin, A., Lisanti, M. P., Scherer, P. E. (1999). Constitutively Active Mitogen-activated Protein Kinase Kinase 6 (MKK6) or Salicylate Induces Spontaneous 3T3-L1 Adipogenesis. J. Biol. Chem. 274: 35630-35638 [Abstract] [Full Text]
Nystrom, F. H., Chen, H., Cong, L.-N., Li, Y., Quon, M. J. (1999). Caveolin-1 Interacts with the Insulin Receptor and Can Differentially Modulate Insulin Signaling in Transfected Cos-7 Cells and Rat Adipose Cells. Mol. Endocrinol. 13: 2013-2024 [Abstract] [Full Text]
Smart, E. J., Graf, G. A., McNiven, M. A., Sessa, W. C., Engelman, J. A., Scherer, P. E., Okamoto, T., Lisanti, M. P. (1999). Caveolins, Liquid-Ordered Domains, and Signal Transduction. Mol. Cell. Biol. 19: 7289-7304 [Full Text]
GUSTAVSSON, J., PARPAL, S., KARLSSON, M., RAMSING, C., THORN, H., BORG, M., LINDROTH, M., PETERSON, K. H., MAGNUSSON, K.-E., STRåLFORS, P. (1999). Localization of the insulin receptor in caveolae of adipocyte plasma membrane. FASEB J. 13: 1961-1971 [Abstract] [Full Text]
Bathori, G., Parolini, I., Tombola, F., Szabo, I., Messina, A., Oliva, M., De Pinto, V., Lisanti, M., Sargiacomo, M., Zoratti, M. (1999). Porin Is Present in the Plasma Membrane Where It Is Concentrated in Caveolae and Caveolae-related Domains. J. Biol. Chem. 274: 29607-29612 [Abstract] [Full Text]
Galbiati, F., Volonte, D., Engelman, J. A., Scherer, P. E., Lisanti, M. P. (1999). Targeted Down-regulation of Caveolin-3 Is Sufficient to Inhibit Myotube Formation in Differentiating C2C12 Myoblasts. TRANSIENT ACTIVATION OF p38 MITOGEN-ACTIVATED PROTEIN KINASE IS REQUIRED FOR INDUCTION OF CAVEOLIN-3 EXPRESSION AND SUBSEQUENT MYOTUBE FORMATION. J. Biol. Chem. 274: 30315-30321 [Abstract] [Full Text]
Razani, B., Rubin, C. S., Lisanti, M. P. (1999). Regulation of cAMP-mediated Signal Transduction via Interaction of Caveolins with the Catalytic Subunit of Protein Kinase A. J. Biol. Chem. 274: 26353-26360 [Abstract] [Full Text]
Galbiati, F., Volonte, D., Minetti, C., Chu, J. B., Lisanti, M. P. (1999). Phenotypic Behavior of Caveolin-3 Mutations That Cause Autosomal Dominant Limb Girdle Muscular Dystrophy (LGMD-1C). RETENTION OF LGMD-1C CAVEOLIN-3 MUTANTS WITHIN THE GOLGI COMPLEX. J. Biol. Chem. 274: 25632-25641 [Abstract] [Full Text]
Parolini, I., Sargiacomo, M., Galbiati, F., Rizzo, G., Grignani, F., Engelman, J. A., Okamoto, T., Ikezu, T., Scherer, P. E., Mora, R., Rodriguez-Boulan, E., Peschle, C., Lisanti, M. P. (1999). Expression of Caveolin-1 Is Required for the Transport of Caveolin-2 to the Plasma Membrane. RETENTION OF CAVEOLIN-2 AT THE LEVEL OF THE GOLGI COMPLEX. J. Biol. Chem. 274: 25718-25725 [Abstract] [Full Text]
Matveev, S., van der Westhuyzen, D. R., Smart, E. J. (1999). Co-expression of scavenger receptor-BI and caveolin-1 is associated with enhanced selective cholesteryl ester uptake in THP-1 macrophages. J. Lipid Res. 40: 1647-1654 [Abstract] [Full Text]
Schlegel, A., Schwab, R. B., Scherer, P. E., Lisanti, M. P. (1999). A Role for the Caveolin Scaffolding Domain in Mediating the Membrane Attachment of Caveolin-1. THE CAVEOLIN SCAFFOLDING DOMAIN IS BOTH NECESSARY AND SUFFICIENT FOR MEMBRANE BINDING IN VITRO. J. Biol. Chem. 274: 22660-22667 [Abstract] [Full Text]
Das, K., Lewis, R. Y., Scherer, P. E., Lisanti, M. P. (1999). The Membrane-spanning Domains of Caveolins-1 and -2�Mediate the Formation of Caveolin Hetero-oligomers. IMPLICATIONS FOR THE ASSEMBLY OF CAVEOLAE MEMBRANES IN VIVO. J. Biol. Chem. 274: 18721-18728 [Abstract] [Full Text]
Stan, R.-V., Ghitescu, L., Jacobson, B. S., Palade, G. E. (1999). Isolation, Cloning, and Localization of Rat PV-1, a Novel Endothelial Caveolar Protein. JCB 145: 1189-1198 [Abstract] [Full Text]
Volonte, D., Galbiati, F., Li, S., Nishiyama, K., Okamoto, T., Lisanti, M. P. (1999). Flotillins/Cavatellins Are Differentially Expressed in Cells and Tissues and Form a Hetero-oligomeric Complex with Caveolins in Vivo. CHARACTERIZATION AND EPITOPE-MAPPING OF A NOVEL FLOTILLIN-1 MONOCLONAL ANTIBODY PROBE. J. Biol. Chem. 274: 12702-12709 [Abstract] [Full Text]
Bradbury, N. A., Clark, J. A., Watkins, S. C., Widnell, C. C., Smith, H. S. IV, Bridges, R. J. (1999). Characterization of the internalization pathways for the cystic fibrosis transmembrane conductance regulator. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L659-L668 [Abstract] [Full Text]
El-Jack, A. K., Hamm, J. K., Pilch, P. F., Farmer, S. R. (1999). Reconstitution of Insulin-sensitive Glucose Transport in Fibroblasts Requires Expression of Both PPARgamma and C/EBPalpha. J. Biol. Chem. 274: 7946-7951 [Abstract] [Full Text]
Gilbert, A, Paccaud, J., Foti, M, Porcheron, G, Balz, J, Carpentier, J. (1999). Direct demonstration of the endocytic function of caveolae by a cell-free assay. J. Cell Sci. 112: 1101-1110 [Abstract]
Valentijn, K., Gumkowski, F., Jamieson, J. (1999). The subapical actin cytoskeleton regulates secretion and membrane retrieval in pancreatic acinar cells. J. Cell Sci. 112: 81-96 [Abstract]
Pitterle, D. M., Sperling, R. T., Myers, M. G. Jr., White, M. F., Blackshear, P. J. (1999). Early biochemical events in insulin-stimulated fluid phase endocytosis. Am. J. Physiol. Endocrinol. Metab. 276: E94-E105 [Abstract] [Full Text]
Engelman, J. A., Lisanti, M. P., Scherer, P. E. (1998). Specific Inhibitors of p38 Mitogen-activated Protein Kinase Block 3T3-L1 Adipogenesis. J. Biol. Chem. 273: 32111-32120 [Abstract] [Full Text]
Field, F. J., Born, E., Murthy, S., Mathur, S. N. (1998). Caveolin is present in intestinal cells: role in cholesterol trafficking?. J. Lipid Res. 39: 1938-1950 [Abstract] [Full Text]
Galbiati, F., Volonte, D., Gil, O., Zanazzi, G., Salzer, J. L., Sargiacomo, M., Scherer, P. E., Engelman, J. A., Schlegel, A., Parenti, M., Okamoto, T., Lisanti, M. P. (1998). Expression of caveolin-1 and -2�in differentiating PC12 cells and dorsal root ganglion neurons: Caveolin-2 is up-regulated in response to cell injury. Proc. Natl. Acad. Sci. USA 95: 10257-10262 [Abstract] [Full Text]
Al-Hasani, H., Hinck, C. S., Cushman, S. W. (1998). Endocytosis of the Glucose Transporter GLUT4 Is Mediated by the GTPase Dynamin. J. Biol. Chem. 273: 17504-17510 [Abstract] [Full Text]
Esser, S., Wolburg, K., Wolburg, H., Breier, G., Kurzchalia, T., Risau, W. (1998). Vascular Endothelial Growth Factor Induces Endothelial Fenestrations In Vitro. JCB 140: 947-959 [Abstract] [Full Text]
Baldini, G., Baldini, G., Wang, G., Weber, M., Zweyer, M., Bareggi, R., Witkin, J. W., Martelli, A. M. (1998). Expression of Rab3D N135I Inhibits Regulated Secretion of ACTH in AtT-20 Cells. JCB 140: 305-313 [Abstract] [Full Text]
Scherer, P. E., Lewis, R. Y., Volonte, D., Engelman, J. A., Galbiati, F., Couet, J., Kohtz, D. S., van Donselaar, E., Peters, P., Lisanti, M. P. (1997). Cell-type and Tissue-specific Expression of Caveolin-2. CAVEOLINS 1�AND 2�CO-LOCALIZE AND FORM A STABLE HETERO-OLIGOMERIC COMPLEX IN VIVO. J. Biol. Chem. 272: 29337-29346 [Abstract] [Full Text]
Scherer, P. E., Lisanti, M. P. (1997). Association of Phosphofructokinase-M with Caveolin-3 in Differentiated Skeletal Myotubes. DYNAMIC REGULATION BY EXTRACELLULAR GLUCOSE AND INTRACELLULAR METABOLITES. J. Biol. Chem. 272: 20698-20705 [Abstract] [Full Text]
Mastick, C. C., Saltiel, A. R. (1997). Insulin-stimulated Tyrosine Phosphorylation of Caveolin Is Specific for the Differentiated Adipocyte Phenotype in 3T3-L1 Cells. J. Biol. Chem. 272: 20706-20714 [Abstract] [Full Text]
Engelman, J. A., Wykoff, C. C., Yasuhara, S., Song, K. S., Okamoto, T., Lisanti, M. P. (1997). Recombinant Expression of Caveolin-1 in Oncogenically Transformed Cells Abrogates Anchorage-independent Growth. J. Biol. Chem. 272: 16374-16381 [Abstract] [Full Text]