TGF-beta induced transdifferentiation of mammary epithelial cells to mesenchymal cells: involvement of type I receptors

doi:10.1083/jcb.127.6.2021

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TGF-beta induced transdifferentiation of mammary epithelial cells to mesenchymal cells: involvement of type I receptors

PJ Miettinen, R Ebner, AR Lopez and R Derynck
Department of Growth and Development, University of California at San Francisco 94143-0640.

The secreted polypeptide transforming growth factor-beta (TGF-beta) exerts its multiple activities through type I and II cell surface receptors. In epithelial cells, activation of the TGF-beta signal transduction pathways leads to inhibition of cell proliferation and an increase in extracellular matrix production. TGF-beta is widely expressed during development and its biological activity has been implicated in epithelial-mesenchymal interactions, e.g., in branching morphogenesis of the lung, kidney, and mammary gland, and in inductive events between mammary epithelium and stroma. In the present study, we investigated the effects of TGF-beta on mouse mammary epithelial cells in vitro. TGF-beta reversibly induced an alteration in the differentiation of normal mammary epithelial NMuMG cells from epithelial to fibroblastic phenotype. The change in cell morphology correlated with (a) decreased expression of the epithelial markers E- cadherin, ZO-1, and desmoplakin I and II; (b) increased expression of mesenchymal markers, such as fibronectin; and (c) a fibroblast-like reorganization of actin fibers. This phenotypic differentiation displays the hallmarks of an epithelial to mesenchymal transdifferentiation event. Since NMuMG cells make high levels of the type I TGF-beta receptor Tsk7L, yet lack expression of the ALK-5/R4 type I receptor which has been reported to mediate TGF-beta responsiveness, we evaluated the role of the Tsk7L receptor in TGF-beta- mediated transdifferentiation. We generated NMuMG cells that stably overexpress a truncated Tsk7L type I receptor that lacks most of the cytoplasmic kinase domain, thus function as a dominant negative mutant. These transfected cells no longer underwent epithelial to mesenchymal morphological change upon exposure to TGF-beta, yet still displayed some TGF-beta-mediated responses. We conclude that TGF-beta has the ability to modulate E-cadherin expression and induce a reversible epithelial to mesenchymal transdifferentiation in epithelial cells. Unlike other transdifferentiating growth factors, such as bFGF and HGF, these changes are accompanied by growth inhibition. Our results also implicate the Tsk7L type I receptor as mediating the TGF-beta-induced epithelial to mesenchymal transition.
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Neil, J. R., Johnson, K. M., Nemenoff, R. A., Schiemann, W. P. (2008). Cox-2 inactivates Smad signaling and enhances EMT stimulated by TGF-{beta} through a PGE2-dependent mechanisms. Carcinogenesis 29: 2227-2235 [Abstract] [Full Text]
Hirano, M., Hashimoto, S., Yonemura, S., Sabe, H., Aizawa, S. (2008). EPB41L5 functions to post-transcriptionally regulate cadherin and integrin during epithelial-mesenchymal transition. JCB 182: 1217-1230 [Abstract] [Full Text]
Bailey, K. M., Liu, J. (2008). Caveolin-1 Up-regulation during Epithelial to Mesenchymal Transition Is Mediated by Focal Adhesion Kinase. J. Biol. Chem. 283: 13714-13724 [Abstract] [Full Text]
Northey, J. J., Chmielecki, J., Ngan, E., Russo, C., Annis, M. G., Muller, W. J., Siegel, P. M. (2008). Signaling through ShcA Is Required for Transforming Growth Factor {beta}- and Neu/ErbB-2-Induced Breast Cancer Cell Motility and Invasion. Mol. Cell. Biol. 28: 3162-3176 [Abstract] [Full Text]
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Wheelock, M. J., Shintani, Y., Maeda, M., Fukumoto, Y., Johnson, K. R. (2008). Cadherin switching. J. Cell Sci. 121: 727-735 [Abstract] [Full Text]
Bharathy, S., Xie, W., Yingling, J. M., Reiss, M. (2008). Cancer-Associated Transforming Growth Factor {beta} Type II Receptor Gene Mutant Causes Activation of Bone Morphogenic Protein-Smads and Invasive Phenotype. Cancer Res. 68: 1656-1666 [Abstract] [Full Text]
Beltran, M., Puig, I., Pena, C., Garcia, J. M., Alvarez, A. B., Pena, R., Bonilla, F., de Herreros, A. G. (2008). A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition. Genes Dev. 22: 756-769 [Abstract] [Full Text]
Yin, L., Castagnino, P., Assoian, R. K. (2008). ABCG2 Expression and Side Population Abundance Regulated by a Transforming Growth Factor {beta}-Directed Epithelial-Mesenchymal Transition. Cancer Res. 68: 800-807 [Abstract] [Full Text]
Andarawewa, K. L., Erickson, A. C., Chou, W. S., Costes, S. V., Gascard, P., Mott, J. D., Bissell, M. J., Barcellos-Hoff, M. H. (2007). Ionizing Radiation Predisposes Nonmalignant Human Mammary Epithelial Cells to Undergo Transforming Growth Factor {beta} Induced Epithelial to Mesenchymal Transition. Cancer Res. 67: 8662-8670 [Abstract] [Full Text]
Casey, T. M., Mulvey, T. M., Patnode, T. A., Dean, A., Zakrzewska, E., Plaut, K. (2007). Mammary Epithelial Cells Treated Concurrently with TGF-{alpha} and TGF-{beta} Exhibit Enhanced Proliferation and Death. Exp. Biol. Med. 232: 1027-1040 [Abstract] [Full Text]
Willis, B. C., Borok, Z. (2007). TGF-beta-induced EMT: mechanisms and implications for fibrotic lung disease. Am. J. Physiol. Lung Cell. Mol. Physiol. 293: L525-L534 [Abstract] [Full Text]
Shirakihara, T., Saitoh, M., Miyazono, K. (2007). Differential Regulation of Epithelial and Mesenchymal Markers by {delta}EF1 Proteins in Epithelial Mesenchymal Transition Induced by TGF-beta. Mol. Biol. Cell 18: 3533-3544 [Abstract] [Full Text]
Zeisberg, M., Yang, C., Martino, M., Duncan, M. B., Rieder, F., Tanjore, H., Kalluri, R. (2007). Fibroblasts Derive from Hepatocytes in Liver Fibrosis via Epithelial to Mesenchymal Transition. J. Biol. Chem. 282: 23337-23347 [Abstract] [Full Text]
Lamouille, S., Derynck, R. (2007). Cell size and invasion in TGF-{beta} induced epithelial to mesenchymal transition is regulated by activation of the mTOR pathway. JCB 178: 437-451 [Abstract] [Full Text]
Belguise, K., Guo, S., Sonenshein, G. E. (2007). Activation of FOXO3a by the Green Tea Polyphenol Epigallocatechin-3-Gallate Induces Estrogen Receptor {alpha} Expression Reversing Invasive Phenotype of Breast Cancer Cells. Cancer Res. 67: 5763-5770 [Abstract] [Full Text]
Choi, J., Park, S. Y., Joo, C.-K. (2007). Transforming Growth Factor-{beta}1 Represses E-Cadherin Production via Slug Expression in Lens Epithelial Cells. IOVS 48: 2708-2718 [Abstract] [Full Text]
Baumgart, E., Cohen, M. S., Neto, B. S., Jacobs, M. A., Wotkowicz, C., Rieger-Christ, K. M., Biolo, A., Zeheb, R., Loda, M., Libertino, J. A., Summerhayes, I. C. (2007). Identification and Prognostic Significance of an Epithelial-Mesenchymal Transition Expression Profile in Human Bladder Tumors. Clin. Cancer Res. 13: 1685-1694 [Abstract] [Full Text]
Andl, C. D., Fargnoli, B. B., Okawa, T., Bowser, M., Takaoka, M., Nakagawa, H., Klein-Szanto, A., Hua, X., Herlyn, M., Rustgi, A. K. (2006). Coordinated Functions of E-Cadherin and Transforming Growth Factor {beta} Receptor II In vitro and In vivo.. Cancer Res. 66: 9878-9885 [Abstract] [Full Text]
Illman, S. A., Lehti, K., Keski-Oja, J., Lohi, J. (2006). Epilysin (MMP-28) induces TGF-{beta} mediated epithelial to mesenchymal transition in lung carcinoma cells. J. Cell Sci. 119: 3856-3865 [Abstract] [Full Text]
Yang, Y., Pan, X., Lei, W., Wang, J., Shi, J., Li, F., Song, J. (2006). Regulation of Transforming Growth Factor-{beta}1-Induced Apoptosis and Epithelial-to-Mesenchymal Transition by Protein Kinase A and Signal Transducers and Activators of Transcription 3.. Cancer Res. 66: 8617-8624 [Abstract] [Full Text]
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Biswas, S., Criswell, T. L., Wang, S. E., Arteaga, C. L. (2006). Inhibition of Transforming Growth Factor-{beta} Signaling in Human Cancer: Targeting a Tumor Suppressor Network as a Therapeutic Strategy.. Clin. Cancer Res. 12: 4142-4146 [Full Text]
Ge, R., Rajeev, V., Ray, P., Lattime, E., Rittling, S., Medicherla, S., Protter, A., Murphy, A., Chakravarty, J., Dugar, S., Schreiner, G., Barnard, N., Reiss, M. (2006). Inhibition of Growth and Metastasis of Mouse Mammary Carcinoma by Selective Inhibitor of Transforming Growth Factor-{beta} Type I Receptor Kinase In vivo.. Clin. Cancer Res. 12: 4315-4330 [Abstract] [Full Text]
Shintani, Y., Wheelock, M. J., Johnson, K. R. (2006). Phosphoinositide-3 Kinase-Rac1-c-Jun NH2-terminal Kinase Signaling Mediates Collagen I-induced Cell Scattering and Up-Regulation of N-Cadherin Expression in Mouse Mammary Epithelial Cells. Mol. Biol. Cell 17: 2963-2975 [Abstract] [Full Text]
Shin, S. R., Sanchez-Velar, N., Sherr, D. H., Sonenshein, G. E. (2006). 7,12-Dimethylbenz(a)Anthracene Treatment of a c-rel Mouse Mammary Tumor Cell Line Induces Epithelial to Mesenchymal Transition via Activation of Nuclear Factor-{kappa}B.. Cancer Res. 66: 2570-2575 [Abstract] [Full Text]
Deckers, M., van Dinther, M., Buijs, J., Que, I., Lowik, C., van der Pluijm, G., ten Dijke, P. (2006). The Tumor Suppressor Smad4 Is Required for Transforming Growth Factor {beta}-Induced Epithelial to Mesenchymal Transition and Bone Metastasis of Breast Cancer Cells. Cancer Res. 66: 2202-2209 [Abstract] [Full Text]
Lacher, M. D., Tiirikainen, M. I., Saunier, E. F., Christian, C., Anders, M., Oft, M., Balmain, A., Akhurst, R. J., Korn, W. M. (2006). Transforming Growth Factor-{beta} Receptor Inhibition Enhances Adenoviral Infectability of Carcinoma Cells via Up-Regulation of Coxsackie and Adenovirus Receptor in Conjunction with Reversal of Epithelial-Mesenchymal Transition. Cancer Res. 66: 1648-1657 [Abstract] [Full Text]
Sotgia, F., Williams, T. M., Schubert, W., Medina, F., Minetti, C., Pestell, R. G., Lisanti, M. P. (2006). Caveolin-1 Deficiency (-/-) Conveys Premalignant Alterations in Mammary Epithelia, with Abnormal Lumen Formation, Growth Factor Independence, and Cell Invasiveness. Am. J. Pathol. 168: 292-309 [Abstract] [Full Text]
Grimm, S. L., Contreras, A., Barcellos-Hoff, M.-H., Rosen, J. M. (2005). Cell Cycle Defects Contribute to a Block in Hormone-induced Mammary Gland Proliferation in CCAAT/Enhancer-binding Protein (C/EBP{beta})-null Mice. J. Biol. Chem. 280: 36301-36309 [Abstract] [Full Text]
Tumbarello, D. A., Brown, M. C., Hetey, S. E., Turner, C. E. (2005). Regulation of paxillin family members during epithelial-mesenchymal transformation: a putative role for paxillin {delta}. J. Cell Sci. 118: 4849-4863 [Abstract] [Full Text]
Willis, B. C., Liebler, J. M., Luby-Phelps, K., Nicholson, A. G., Crandall, E. D., du Bois, R. M., Borok, Z. (2005). Induction of Epithelial-Mesenchymal Transition in Alveolar Epithelial Cells by Transforming Growth Factor-{beta}1: Potential Role in Idiopathic Pulmonary Fibrosis. Am. J. Pathol. 166: 1321-1332 [Abstract] [Full Text]
Prunier, C., Howe, P. H. (2005). Disabled-2 (Dab2) Is Required for Transforming Growth Factor {beta}-induced Epithelial to Mesenchymal Transition (EMT). J. Biol. Chem. 280: 17540-17548 [Abstract] [Full Text]
Valcourt, U., Kowanetz, M., Niimi, H., Heldin, C.-H., Moustakas, A. (2005). TGF-{beta} and the Smad Signaling Pathway Support Transcriptomic Reprogramming during Epithelial-Mesenchymal Cell Transition. Mol. Biol. Cell 16: 1987-2002 [Abstract] [Full Text]
Elliott, R. L., Blobe, G. C. (2005). Role of Transforming Growth Factor Beta in Human Cancer. JCO 23: 2078-2093 [Abstract] [Full Text]
Ozdamar, B., Bose, R., Barrios-Rodiles, M., Wang, H.-R., Zhang, Y., Wrana, J. L. (2005). Regulation of the Polarity Protein Par6 by TGF{beta} Receptors Controls Epithelial Cell Plasticity. Science 307: 1603-1609 [Abstract] [Full Text]
Maeda, M., Johnson, K. R., Wheelock, M. J. (2005). Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition. J. Cell Sci. 118: 873-887 [Abstract] [Full Text]
Muraoka-Cook, R. S., Dumont, N., Arteaga, C. L. (2005). Dual Role of Transforming Growth Factor ss in Mammary Tumorigenesis and Metastatic Progression. Clin. Cancer Res. 11: 937s-943s [Abstract] [Full Text]
Barcellos-Hoff, M H (2005). How tissues respond to damage at the cellular level: orchestration by transforming growth factor-{beta} (TGF-{beta}). Br. J. Radiol. Supplement_27: 123-127 [Abstract] [Full Text]
Muraoka-Cook, R. S., Kurokawa, H., Koh, Y., Forbes, J. T., Roebuck, L. R., Barcellos-Hoff, M. H., Moody, S. E., Chodosh, L. A., Arteaga, C. L. (2004). Conditional Overexpression of Active Transforming Growth Factor {beta}1 In vivo Accelerates Metastases of Transgenic Mammary Tumors. Cancer Res. 64: 9002-9011 [Abstract] [Full Text]
Prime, S.S., Davies, M., Pring, M., Paterson, I.C. (2004). THE ROLE OF TGF-{beta} IN EPITHELIAL MALIGNANCY AND ITS RELEVANCE TO THE PATHOGENESIS OF ORAL CANCER (PART II). CROBM 15: 337-347 [Abstract] [Full Text]
Mori, K., Shibanuma, M., Nose, K. (2004). Invasive Potential Induced under Long-Term Oxidative Stress in Mammary Epithelial Cells. Cancer Res. 64: 7464-7472 [Abstract] [Full Text]
Hinz, B., Pittet, P., Smith-Clerc, J., Chaponnier, C., Meister, J.-J. (2004). Myofibroblast Development Is Characterized by Specific Cell-Cell Adherens Junctions. Mol. Biol. Cell 15: 4310-4320 [Abstract] [Full Text]
Kaartinen, V., Dudas, M., Nagy, A., Sridurongrit, S., Lu, M. M., Epstein, J. A. (2004). Cardiac outflow tract defects in mice lacking ALK2 in neural crest cells. Development 131: 3481-3490 [Abstract] [Full Text]
Ohkubo, T., Ozawa, M. (2004). The transcription factor Snail downregulates the tight junction components independently of E-cadherin downregulation. J. Cell Sci. 117: 1675-1685 [Abstract] [Full Text]
Prindull, G., Zipori, D. (2004). Environmental guidance of normal and tumor cell plasticity: epithelial mesenchymal transitions as a paradigm. Blood 103: 2892-2899 [Abstract] [Full Text]
Takeda, M., Mizuide, M., Oka, M., Watabe, T., Inoue, H., Suzuki, H., Fujita, T., Imamura, T., Miyazono, K., Miyazawa, K. (2004). Interaction with Smad4 Is Indispensable for Suppression of BMP Signaling by c-Ski. Mol. Biol. Cell 15: 963-972 [Abstract] [Full Text]
Seton-Rogers, S. E., Lu, Y., Hines, L. M., Koundinya, M., LaBaer, J., Muthuswamy, S. K., Brugge, J. S. (2004). Cooperation of the ErbB2 receptor and transforming growth factor {beta} in induction of migration and invasion in mammary epithelial cells. Proc. Natl. Acad. Sci. USA 101: 1257-1262 [Abstract] [Full Text]
Nawshad, A., Hay, E. D. (2003). TGF{beta}3 signaling activates transcription of the LEF1 gene to induce epithelial mesenchymal transformation during mouse palate development. JCB 163: 1291-1301 [Abstract] [Full Text]
Wada, A. M., Willet, S. G., Bader, D. (2003). Coronary Vessel Development: A Unique Form of Vasculogenesis. Arterioscler. Thromb. Vasc. Bio. 23: 2138-2145 [Abstract] [Full Text]
Kurisaki, K., Kurisaki, A., Valcourt, U., Terentiev, A. A., Pardali, K., ten Dijke, P., Heldin, C.-H., Ericsson, J., Moustakas, A. (2003). Nuclear Factor YY1 Inhibits Transforming Growth Factor {beta}- and Bone Morphogenetic Protein-Induced Cell Differentiation. Mol. Cell. Biol. 23: 4494-4510 [Abstract] [Full Text]
Peinado, H., Quintanilla, M., Cano, A. (2003). Transforming Growth Factor {beta}-1 Induces Snail Transcription Factor in Epithelial Cell Lines: MECHANISMS FOR EPITHELIAL MESENCHYMAL TRANSITIONS. J. Biol. Chem. 278: 21113-21123 [Abstract] [Full Text]
Saika, S., Miyamoto, T., Tanaka, S.-i., Tanaka, T., Ishida, I., Ohnishi, Y., Ooshima, A., Ishiwata, T., Asano, G., Chikama, T.-i., Shiraishi, A., Liu, C.-Y., Kao, C. W.-C., Kao, W. W.-Y. (2003). Response of Lens Epithelial Cells to Injury: Role of Lumican in Epithelial-Mesenchymal Transition. IOVS 44: 2094-2102 [Abstract] [Full Text]
Mareel, M., Leroy, A. (2003). Clinical, Cellular, and Molecular Aspects of Cancer Invasion. Physiol. Rev. 83: 337-376 [Abstract] [Full Text]
Itoh, S., Thorikay, M., Kowanetz, M., Moustakas, A., Itoh, F., Heldin, C.-H., ten Dijke, P. (2003). Elucidation of Smad Requirement in Transforming Growth Factor-beta Type I Receptor-induced Responses. J. Biol. Chem. 278: 3751-3761 [Abstract] [Full Text]
Ward, S. M., Desgrosellier, J. S., Zhuang, X., Barnett, J. V., Galper, J. B. (2002). Transforming Growth Factor beta (TGFbeta ) Signaling via Differential Activation of Activin Receptor-like Kinases 2 and 5 during Cardiac Development. ROLE IN REGULATING PARASYMPATHETIC RESPONSIVENESS. J. Biol. Chem. 277: 50183-50189 [Abstract] [Full Text]
Tsubouchi, A., Sakakura, J., Yagi, R., Mazaki, Y., Schaefer, E., Yano, H., Sabe, H. (2002). Localized suppression of RhoA activity by Tyr31/118-phosphorylated paxillin in cell adhesion and migration. JCB 159: 673-683 [Abstract] [Full Text]
Grande, M., Franzen, A., Karlsson, J.-O., Ericson, L. E., Heldin, N.-E., Nilsson, M. (2002). Transforming growth factor-{beta} and epidermal growth factor synergistically stimulate epithelial to mesenchymal transition (EMT) through a MEK-dependent mechanism in primary cultured pig thyrocytes. J. Cell Sci. 115: 4227-4236 [Abstract] [Full Text]
Valdes, F., Alvarez, A. M., Locascio, A., Vega, S., Herrera, B., Fernandez, M., Benito, M., Nieto, M. A., Fabregat, I. (2002). The Epithelial Mesenchymal Transition Confers Resistance to the Apoptotic Effects of Transforming Growth Factor {beta} in Fetal Rat Hepatocytes. Mol Cancer Res 1: 68-78 [Abstract] [Full Text]
Bottinger, E. P., Bitzer, M. (2002). TGF-{beta} Signaling in Renal Disease. J. Am. Soc. Nephrol. 13: 2600-2610 [Abstract] [Full Text]
Oliver, J. A., Barasch, J., Yang, J., Herzlinger, D., Al-Awqati, Q. (2002). Metanephric mesenchyme contains embryonic renal stem cells. Am. J. Physiol. Renal Physiol. 283: F799-F809 [Abstract] [Full Text]
Kim, J.-T., Joo, C.-K. (2002). Involvement of Cell-Cell Interactions in the Rapid Stimulation of Cas Tyrosine Phosphorylation and Src Kinase Activity by Transforming Growth Factor-beta 1. J. Biol. Chem. 277: 31938-31948 [Abstract] [Full Text]
Frid, M. G., Kale, V. A., Stenmark, K. R. (2002). Mature Vascular Endothelium Can Give Rise to Smooth Muscle Cells via Endothelial-Mesenchymal Transdifferentiation: In Vitro Analysis. Circ. Res. 90: 1189-1196 [Abstract] [Full Text]
Tian, Y. C., Phillips, A. O. (2002). Interaction between the Transforming Growth Factor-{beta} Type II Receptor/Smad Pathway and {beta}-Catenin during Transforming Growth Factor-{beta}1-Mediated Adherens Junction Disassembly. Am. J. Pathol. 160: 1619-1628 [Abstract] [Full Text]
Biasi, F, Tessitore, L, Zanetti, D, Cutrin, J C, Zingaro, B, Chiarpotto, E, Zarkovic, N, Serviddio, G, Poli, G (2002). Associated changes of lipid peroxidation and transforming growth factor {beta}1 levels in human colon cancer during tumour progression. Gut 50: 361-367 [Abstract] [Full Text]
Edlund, S., Landstrom, M., Heldin, C.-H., Aspenstrom, P. (2002). Transforming Growth Factor-beta -induced Mobilization of Actin Cytoskeleton Requires Signaling by Small GTPases Cdc42 and RhoA. Mol. Biol. Cell 13: 902-914 [Abstract] [Full Text]
Bakin, A. V., Rinehart, C., Tomlinson, A. K., Arteaga, C. L. (2002). p38 mitogen-activated protein kinase is required for TGF{beta}-mediated fibroblastic transdifferentiation and cell migration. J. Cell Sci. 115: 3193-3206 [Abstract] [Full Text]
Bhowmick, N. A., Zent, R., Ghiassi, M., McDonnell, M., Moses, H. L. (2001). Integrin beta 1 Signaling Is Necessary for Transforming Growth Factor-beta Activation of p38MAPK and Epithelial Plasticity. J. Biol. Chem. 276: 46707-46713 [Abstract] [Full Text]
Shin, I., Bakin, A. V., Rodeck, U., Brunet, A., Arteaga, C. L. (2001). Transforming Growth Factor beta Enhances Epithelial Cell Survival via Akt-dependent Regulation of FKHRL1. Mol. Biol. Cell 12: 3328-3339 [Abstract] [Full Text]
Stahl, P. J., Felsen, D. (2001). Transforming Growth Factor-{beta}, Basement Membrane, and Epithelial-Mesenchymal Transdifferentiation : Implications for Fibrosis in Kidney Disease. Am. J. Pathol. 159: 1187-1192 [Full Text]
Zavadil, J., Bitzer, M., Liang, D., Yang, Y.-C., Massimi, A., Kneitz, S., Piek, E., Bottinger, E. P. (2001). Genetic programs of epithelial cell plasticity directed by transforming growth factor-beta. Proc. Natl. Acad. Sci. USA 98: 6686-6691 [Abstract] [Full Text]
Ellenrieder, V., Hendler, S. F., Boeck, W., Seufferlein, T., Menke, A., Ruhland, C., Adler, G., Gress, T. M. (2001). Transforming Growth Factor {beta}1 Treatment Leads to an Epithelial-Mesenchymal Transdifferentiation of Pancreatic Cancer Cells Requiring Extracellular Signal-regulated Kinase 2 Activation. Cancer Res. 61: 4222-4228 [Abstract] [Full Text]
Decline, F, Rousselle, P (2001). Keratinocyte migration requires alpha2beta1 integrin-mediated interaction with the laminin 5 gamma2 chain. J. Cell Sci. 114: 811-823 [Abstract]
Bhowmick, N. A., Ghiassi, M., Bakin, A., Aakre, M., Lundquist, C. A., Engel, M. E., Arteaga, C. L., Moses, H. L. (2001). Transforming Growth Factor-{beta}1 Mediates Epithelial to Mesenchymal Transdifferentiation through a RhoA-dependent Mechanism. Mol. Biol. Cell 12: 27-36 [Abstract] [Full Text]
Yang, S. P., Woolf, A. S., Yuan, H. T., Scott, R. J., Risdon, R. A., O'Hare, M. J., Winyard, P. J. D. (2000). Potential Biological Role of Transforming Growth Factor-{beta}1 in Human Congenital Kidney Malformations. Am. J. Pathol. 157: 1633-1647 [Abstract] [Full Text]
Yano, H., Uchida, H., Iwasaki, T., Mukai, M., Akedo, H., Nakamura, K., Hashimoto, S., Sabe, H. (2000). Paxillin alpha and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation. Proc. Natl. Acad. Sci. USA 97: 9076-9081 [Abstract] [Full Text]
Roy, S. K. (2000). Regulation of Transforming Growth Factor-{beta}-Receptor Type I and Type II Messenger Ribonucleic Acid Expression in the Hamster Ovary by Gonadotropinsand Steroid Hormones. Biol. Reprod. 62: 1858-1865 [Abstract] [Full Text]
Barcellos-Hoff, M. H., Ravani, S. A. (2000). Irradiated Mammary Gland Stroma Promotes the Expression of Tumorigenic Potential by Unirradiated Epithelial Cells. Cancer Res. 60: 1254-1260 [Abstract] [Full Text]
Batard, P, Monier, M., Fortunel, N, Ducos, K, Sansilvestri-Morel, P, Phan, T, Hatzfeld, A, Hatzfeld, J. (2000). TGF-(beta)1 maintains hematopoietic immaturity by a reversible negative control of cell cycle and induces CD34 antigen up-modulation. J. Cell Sci. 113: 383-390 [Abstract]
Ng, Y.-Y., Fan, J.-M., Mu, W., Nikolic-Paterson, D. J., Yang, W.-C., Huang, T.-p., Atkins, R. C., Lan, H. Y. (1999). Glomerular epithelial-myofibroblast transdifferentiation in the evolution of glomerular crescent formation. Nephrol Dial Transplant 14: 2860-2872 [Abstract] [Full Text]
Nieman, M. T., Prudoff, R. S., Johnson, K. R., Wheelock, M. J. (1999). N-Cadherin Promotes Motility in Human Breast Cancer Cells Regardless of Their E-Cadherin Expression. JCB 147: 631-644 [Abstract] [Full Text]
Lee, E. H., Joo, C.-K. (1999). Role of Transforming Growth Factor-{beta} in Transdifferentiation and Fibrosis of Lens Epithelial Cells. IOVS 40: 2025-2032 [Abstract] [Full Text]
Montesano, R., Soriano, J. V., Hosseini, G., Pepper, M. S., Schramek, H. (1999). Constitutively Active Mitogen-activated Protein Kinase Kinase MEK1 Disrupts Morphogenesis and Induces an Invasive Phenotype in Madin-Darby Canine Kidney Epithelial Cells. Cell Growth Differ. 10: 317-332 [Abstract] [Full Text]
Pötter, E., Bergwitz, C., Brabant, G. (1999). The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues. Endocr. Rev. 20: 207-239 [Abstract] [Full Text]
Piek, E, Moustakas, A, Kurisaki, A, Heldin, C., ten Dijke, P (1999). TGF-(beta) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells. J. Cell Sci. 112: 4557-4568 [Abstract]
Filvaroff, E, Erlebacher, A, Ye, J, Gitelman, S., Lotz, J, Heillman, M, Derynck, R (1999). Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development 126: 4267-4279 [Abstract]
Danielpour, D (1999). Transdifferentiation of NRP-152 rat prostatic basal epithelial cells toward a luminal phenotype: regulation by glucocorticoid, insulin-like growth factor-I and transforming growth factor-beta. J. Cell Sci. 112: 169-179 [Abstract]
Griswold-Prenner, I., Kamibayashi, C., Maruoka, E. M., Mumby, M. C., Derynck, R. (1998). Physical and Functional Interactions between Type I Transforming Growth Factor beta �Receptors and Balpha , a WD-40 Repeat Subunit of Phosphatase 2A. Mol. Cell. Biol. 18: 6595-6604 [Abstract] [Full Text]