The mucin epiglycanin on TA3/Ha carcinoma cells prevents alpha 6 beta 4- mediated adhesion to laminin and kalinin and E-cadherin-mediated cell- cell interaction

doi:10.1083/jcb.127.6.2071

This Article

	Full Text (PDF, 3159K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kemperman, H.
	Articles by Roos, E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kemperman, H.
	Articles by Roos, E.


Social Bookmarking

	What's this?

ARTICLES

The mucin epiglycanin on TA3/Ha carcinoma cells prevents alpha 6 beta 4- mediated adhesion to laminin and kalinin and E-cadherin-mediated cell- cell interaction

H Kemperman, Y Wijnands, J Wesseling, CM Niessen, A Sonnenberg and E Roos
Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam.

TA3/Ha murine mammary carcinoma cells grow in suspension, do not adhere to extracellular matrix molecules, but do adhere to hepatocytes and form liver metastases upon intraportal injection. Recently we showed that the integrin alpha 6 beta 4 on the TA3/Ha cells is involved in adhesion to hepatocytes. However, despite high cell surface levels of alpha 6 beta 4, TA3/Ha cells do not adhere to the alpha 6 beta 4 ligands laminin and kalinin. Here we show that this is due to the mucin epiglycanin that is highly expressed on TA3/Ha cells. Some monoclonal antibodies generated against epiglycanin induced capping of most of the epiglycanin molecules. TA3/Ha cells treated with these mAb did adhere to laminin and kalinin, and an epithelial monolayer was formed on kalinin, with alpha 6 beta 4 localized in HD1-containing hemidesmosome- like structures and E-cadherin at the cell-cell contact sites. Similar results were obtained after treatment of TA3/Ha cells with O- sialoglycoprotein endopeptidase which removes all epiglycanin. In addition, the enzyme induced E-cadherin-mediated cell-cell aggregation. Both treatments also enhanced the adhesion to hepatocytes, but given the potent antiadhesive effect of epiglycanin it is remarkable that nontreated TA3/Ha cells adhere to hepatocytes at all. We found that during this interaction, epiglycanin was redistributed. We conclude that epiglycanin can completely prevent both intercellular and matrix adhesion, but that this effect can be overcome in certain intercellular interactions because of the induced redistribution of the mucin.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Itoh, Y., Kamata-Sakurai, M., Denda-Nagai, K., Nagai, S., Tsuiji, M., Ishii-Schrade, K., Okada, K., Goto, A., Fukayama, M., Irimura, T. (2008). Identification and Expression of Human Epiglycanin/MUC21: a Novel Transmembrane Mucin. Glycobiology 18: 74-83 [Abstract] [Full Text]
Economou, C. G., Kitsiou, P. V., Tzinia, A. K., Panagopoulou, E., Marinos, E., Kershaw, D. B., Kerjaschki, D., Tsilibary, E. C. (2004). Enhanced podocalyxin expression alters the structure of podocyte basal surface. J. Cell Sci. 117: 3281-3294 [Abstract] [Full Text]
Baldus, S. E., Monig, S. P., Huxel, S., Landsberg, S., Hanisch, F.-G., Engelmann, K., Schneider, P. M., Thiele, J., Holscher, A. H., Dienes, H. P. (2004). MUC1 and Nuclear {beta}-Catenin Are Coexpressed at the Invasion Front of Colorectal Carcinomas and Are Both Correlated with Tumor Prognosis. Clin. Cancer Res. 10: 2790-2796 [Abstract] [Full Text]
Tsuiji, H., Takasaki, S., Sakamoto, M., Irimura, T., Hirohashi, S. (2003). Aberrant O-glycosylation inhibits stable expression of dysadherin, a carcinoma-associated antigen, and facilitates cell-cell adhesion. Glycobiology 13: 521-527 [Abstract] [Full Text]
Van Aken, E. H., Papeleu, P., Patrick De Potter, , Bruyneel, E., Philippe, J., Seregard, S., Kvanta, A., De Laey, J.-J., Mareel, M. M. (2002). Structure and Function of the N-Cadherin/Catenin Complex in Retinoblastoma. IOVS 43: 595-602 [Abstract] [Full Text]
Ulloa, F., Real, F. X. (2001). Differential Distribution of Sialic Acid in {{alpha}}2,3 and {{alpha}}2,6 Linkages in the Apical Membrane of Cultured Epithelial Cells and Tissues. J. Histochem. Cytochem. 49: 501-510 [Abstract] [Full Text]
Takeda, T., Go, W. Y., Orlando, R. A., Farquhar, M. G. (2000). Expression of Podocalyxin Inhibits Cell-Cell Adhesion and Modifies Junctional Properties in Madin-Darby Canine Kidney Cells. Mol. Biol. Cell 11: 3219-3232 [Abstract] [Full Text]
Chervenak, J. L., Illsley, N. P. (2000). Episialin Acts as an Antiadhesive Factor in an In Vitro Model of Human Endometrial-Blastocyst Attachment. Biol. Reprod. 63: 294-300 [Abstract] [Full Text]
Abe, Y., Smith, C. W., Katkin, J. P., Thurmon, L. M., Xu, X., Mendoza, L. H., Ballantyne, C. M. (1999). Endothelial {alpha}2,6-Linked Sialic Acid Inhibits VCAM-1- Dependent Adhesion Under Flow Conditions. J. Immunol. 163: 2867-2876 [Abstract] [Full Text]
Aono, S., Nakagawa, S., Reynolds, A. B., Takeichi, M. (1999). p120ctn Acts as an Inhibitory Regulator of Cadherin Function in Colon Carcinoma Cells. JCB 145: 551-562 [Abstract] [Full Text]
Scholl, F., Gamallo, C, Vilar?o, S, Quintanilla, M (1999). Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein that induces plasma membrane extensions and increased motility in keratinocytes. J. Cell Sci. 112: 4601-4613 [Abstract]
Morgan, S. M., Samulowitz, U., Darley, L., Simmons, D. L., Vestweber, D. (1999). Biochemical Characterization and Molecular Cloning of a Novel Endothelial-Specific Sialomucin. Blood 93: 165-175 [Abstract] [Full Text]
Nievers, M., Schaapveld, R., Oomen, L., Fontao, L, Geerts, D, Sonnenberg, A (1998). Ligand-independent role of the beta 4 integrin subunit in the formation of hemidesmosomes. J. Cell Sci. 111: 1659-1672 [Abstract]
Komatsu, M., Carraway, C. A.�C., Fregien, N. L., Carraway, K. L. (1997). Reversible Disruption of Cell-Matrix and Cell-Cell Interactions by Overexpression of Sialomucin Complex. J. Biol. Chem. 272: 33245-33254 [Abstract] [Full Text]
Capon, C., Wieruszeski, J.-M., Lemoine, J., Byrd, J. C., Leffler, H., Kim, Y. S. (1997). Sulfated Lewis X Determinants as a Major Structural Motif in Glycans from LS174T-HM7 Human Colon Carcinoma Mucin. J. Biol. Chem. 272: 31957-31968 [Abstract] [Full Text]
Niessen, C., Hulsman, E., Oomen, L., Kuikman, I, Sonnenberg, A (1997). A minimal region on the integrin beta4 subunit that is critical to its localization in hemidesmosomes regulates the distribution of HD1/plectin in COS-7 cells. J. Cell Sci. 110: 1705-1716 [Abstract]
Apperson, M. L., Moon, I. S., Kennedy, M. B. (1996). Characterization of Densin-180, a New Brain-Specific Synaptic Protein of the O-Sialoglycoprotein Family. J. Neurosci. 16: 6839-6852 [Abstract] [Full Text]