The Journal of Cell Biology, Vol 128, 485-498, Copyright © 1995 by The Rockefeller University Press

ARTICLES

Aspergillus nidulans apsA (anucleate primary sterigmata) encodes a coiled-coil protein required for nuclear positioning and completion of asexual development

R Fischer and WE Timberlake
Department of Genetics, University of Georgia, Athens 30602.

Many fungi are capable of growing by polarized cellular extension to form hyphae or by isotropic expansion to form buds. Aspergillus nidulans anucleate primary sterigmata (apsA) mutants are defective in nuclear distribution in both hyphae and in specialized, multicellular reproductive structures, called conidiophores. apsA mutations have a negligible effect on hyphal growth, unlike another class of nuclear distribution (nud) mutants. By contrast, they almost completely block entry of nuclei into primary buds, or sterigmata (bud nucleation), produced during development of conidiophores. Failure of the primary sterigmata to become nucleated results in developmental arrest and a failure to activate the transcriptional program associated with downstream developmental steps. However, occasionally in mutants a nucleus enters a primary bud and this event relieves the developmental blockage. Thus, there is a stringent developmental requirement for apsA function, but only at the stage of primary bud formation. apsA encodes a 183-kD coiled-coil protein with similarity to Saccharomyces cerevisiae NUM1p, required for nuclear migration in the budding process.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Yamashita, A., Yamamoto, M. (2006). Fission Yeast Num1p Is a Cortical Factor Anchoring Dynein and Is Essential for the Horse-Tail Nuclear Movement During Meiotic Prophase. Genetics 173: 1187-1196 [Abstract] [Full Text]  
• Saito, T. T., Okuzaki, D., Nojima, H. (2006). Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast. JCB 173: 27-33 [Abstract] [Full Text]  
• Efimov, V. P., Zhang, J., Xiang, X. (2006). CLIP-170 Homologue and NUDE Play Overlapping Roles in NUDF Localization in Aspergillus nidulans. Mol. Biol. Cell 17: 2021-2034 [Abstract] [Full Text]  
• Deng, Y., Dong, H., Jin, Q., Dai, C., Fang, Y., Liang, S., Wang, K., Shao, J., Lou, Y., Shi, W., Vakalounakis, D. J., Li, D. (2006). Analysis of Expressed Sequence Tag Data and Gene Expression Profiles Involved in Conidial Germination of Fusarium oxysporum. Appl. Environ. Microbiol. 72: 1667-1671 [Abstract] [Full Text]  
• Veith, D., Scherr, N., Efimov, V. P., Fischer, R. (2005). Role of the spindle-pole-body protein ApsB and the cortex protein ApsA in microtubule organization and nuclear migration in Aspergillus nidulans. J. Cell Sci. 118: 3705-3716 [Abstract] [Full Text]  
• Konzack, S., Rischitor, P. E., Enke, C., Fischer, R. (2005). The Role of the Kinesin Motor KipA in Microtubule Organization and Polarized Growth of Aspergillus nidulans. Mol. Biol. Cell 16: 497-506 [Abstract] [Full Text]  
• Rischitor, P. E., Konzack, S., Fischer, R. (2004). The Kip3-Like Kinesin KipB Moves along Microtubules and Determines Spindle Position during Synchronized Mitoses in Aspergillus nidulans Hyphae. Eukaryot Cell 3: 632-645 [Abstract] [Full Text]  
• Bouhouche, K., Zickler, D., Debuchy, R., Arnaise, S. (2004). Altering a Gene Involved in Nuclear Distribution Increases the Repeat-Induced Point Mutation Process in the Fungus Podospora anserina. Genetics 167: 151-159 [Abstract] [Full Text]  
• Yamamoto, A., Hiraoka, Y. (2003). Cytoplasmic dynein in fungi: insights from nuclear migration. J. Cell Sci. 116: 4501-4512 [Abstract] [Full Text]  
• Oberparleiter, C., Kaiserer, L., Haas, H., Ladurner, P., Andratsch, M., Marx, F. (2003). Active Internalization of the Penicillium chrysogenum Antifungal Protein PAF in Sensitive Aspergilli. Antimicrob. Agents Chemother. 47: 3598-3601 [Abstract] [Full Text]  
• Boyce, K. J., Hynes, M. J., Andrianopoulos, A. (2003). Control of morphogenesis and actin localization by the Penicillium marneffei RAC homolog. J. Cell Sci. 116: 1249-1260 [Abstract] [Full Text]  
• Efimov, V. P. (2003). Roles of NUDE and NUDF Proteins of Aspergillus nidulans: Insights from Intracellular Localization and Overexpression Effects. Mol. Biol. Cell 14: 871-888 [Abstract] [Full Text]  
• Mankel, A., Krause, K., Kothe, E. (2002). Identification of a Hydrophobin Gene That is Developmentally Regulated in the Ectomycorrhizal Fungus Tricholoma terreum. Appl. Environ. Microbiol. 68: 1408-1413 [Abstract] [Full Text]  
• Efimov, V. P., Morris, N. R. (2000). The Lis1-Related Nudf Protein of Aspergillus nidulans Interacts with the Coiled-Coil Domain of the Nude/Ro11 Protein. JCB 150: 681-688 [Abstract] [Full Text]  
• Graïa, F., Berteaux-Lecellier, V., Zickler, D., Picard, M. (2000). ami1, an Orthologue of the Aspergillus nidulans apsA Gene, Is Involved in Nuclear Migration Events Throughout the Life Cycle of Podospora anserina. Genetics 155: 633-646 [Abstract] [Full Text]  
• Morris, N. R. (2000). Nuclear Migration: From Fungi to the Mammalian Brain. JCB 148: 1097-1102 [Full Text]  
• Adams, T. H., Wieser, J. K., Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiol. Mol. Biol. Rev. 62: 35-54 [Abstract] [Full Text]  
• Reinsch, S, Gonczy, P (1998). Mechanisms of nuclear positioning. J. Cell Sci. 111: 2283-2295 [Abstract]  
• Wolkow, T., Harris, S., Hamer, J. (1996). Cytokinesis in Aspergillus nidulans is controlled by cell size, nuclear positioning and mitosis. J. Cell Sci. 109: 2179-2188 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents