Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation

doi:10.1083/jcb.128.4.617

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Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation

WS Saunders, D Koshland, D Eshel, IR Gibbons and MA Hoyt
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.

The Saccharomyces cerevisiae kinesin-related gene products Cin8p and Kip1p function to assemble the bipolar mitotic spindle. The cytoplasmic dynein heavy chain homologue Dyn1p (also known as Dhc1p) participates in proper cellular positioning of the spindle. In this study, the roles of these motor proteins in anaphase chromosome segregation were examined. While no single motor was essential, loss of function of all three completely halted anaphase chromatin separation. As combined motor activity was diminished by mutation, both the velocity and extent of chromatin movement were reduced, suggesting a direct role for all three motors in generating a chromosome-separating force. Redundancy for function between different types of microtubule-based motor proteins was also indicated by the observation that cin8 dyn1 double- deletion mutants are inviable. Our findings indicate that the bulk of anaphase chromosome segregation in S. cerevisiae is accomplished by the combined actions of these three motors.
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Mohl, D. A., Huddleston, M. J., Collingwood, T. S., Annan, R. S., Deshaies, R. J. (2009). Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. JCB 184: 527-539 [Abstract] [Full Text]
Sherwood, R. K., Bennett, R. J. (2008). Microtubule Motor Protein Kar3 Is Required for Normal Mitotic Division and Morphogenesis in Candida albicans. Eukaryot Cell 7: 1460-1474 [Abstract] [Full Text]
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Amaro, I. A., Costanzo, M., Boone, C., Huffaker, T. C. (2008). The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex. Genetics 178: 703-709 [Abstract] [Full Text]
Hildebrandt, E. R., Gheber, L., Kingsbury, T., Hoyt, M. A. (2006). Homotetrameric Form of Cin8p, a Saccharomyces cerevisiae Kinesin-5 Motor, Is Essential for Its in Vivo Function. J. Biol. Chem. 281: 26004-26013 [Abstract] [Full Text]
Lui, D. Y., Peoples-Holst, T. L., Chang Mell, J., Wu, H.-Y., Dean, E. W., Burgess, S. M. (2006). Analysis of Close Stable Homolog Juxtaposition During Meiosis in Mutants of Saccharomyces cerevisiae. Genetics 173: 1207-1222 [Abstract] [Full Text]
Li, S., Oakley, C. E., Chen, G., Han, X., Oakley, B. R., Xiang, X. (2005). Cytoplasmic Dynein's Mitotic Spindle Pole Localization Requires a Functional Anaphase-promoting Complex, {gamma}-Tubulin, and NUDF/LIS1 in Aspergillus nidulans. Mol. Biol. Cell 16: 3591-3605 [Abstract] [Full Text]
Yamamoto, A., Hiraoka, Y. (2003). Cytoplasmic dynein in fungi: insights from nuclear migration. J. Cell Sci. 116: 4501-4512 [Abstract] [Full Text]
Hildebrandt, E. R., Hoyt, M. A. (2001). Cell Cycle-dependent Degradation of the Saccharomyces cerevisiae Spindle Motor Cin8p Requires APCCdh1 and a Bipartite Destruction Sequence. Mol. Biol. Cell 12: 3402-3416 [Abstract] [Full Text]
Troxell, C. L., Sweezy, M. A., West, R. R., Reed, K. D., Carson, B. D., Pidoux, A. L., Cande, W. Z., McIntosh, J. R. (2001). pkl1+and klp2+: Two Kinesins of the Kar3 Subfamily in Fission Yeast Perform Different Functions in Both Mitosis and Meiosis. Mol. Biol. Cell 12: 3476-3488 [Abstract] [Full Text]
Farkasovsky, M., Kuntzel, H. (2001). Cortical Num1p Interacts with the Dynein Intermediate Chain Pac11p and Cytoplasmic Microtubules in Budding Yeast. JCB 152: 251-262 [Abstract] [Full Text]
Schuyler, S., Pellman, D (2001). Search, capture and signal: games microtubules and centrosomes play. J. Cell Sci. 114: 247-255 [Abstract]
Paluh, J. L., Nogales, E., Oakley, B. R., McDonald, K., Pidoux, A. L., Cande, W. Z. (2000). A Mutation in gamma -Tubulin Alters Microtubule Dynamics and Organization and Is Synthetically Lethal with the Kinesin-like Protein Pkl1p. Mol. Biol. Cell 11: 1225-1239 [Abstract] [Full Text]
Sharp, D. J., Brown, H. M., Kwon, M., Rogers, G. C., Holland, G., Scholey, J. M. (2000). Functional Coordination of Three Mitotic Motors in Drosophila Embryos. Mol. Biol. Cell 11: 241-253 [Abstract] [Full Text]
Cottingham, F. R., Gheber, L., Miller, D. L., Hoyt, M. A. (1999). Novel Roles for Saccharomyces cerevisiae Mitotic Spindle Motors. JCB 147: 335-350 [Abstract] [Full Text]
Gordon, D. M., Roof, D. M. (1999). The Kinesin-related Protein Kip1p of Saccharomyces cerevisiae Is Bipolar. J. Biol. Chem. 274: 28779-28786 [Abstract] [Full Text]
Robinson, J. T., Wojcik, E. J., Sanders, M. A., McGrail, M., Hays, T. S. (1999). Cytoplasmic Dynein Is Required for the Nuclear Attachment and Migration of Centrosomes during Mitosis in Drosophila. JCB 146: 597-608 [Abstract] [Full Text]
Bowman, A. B., Patel-King, R. S., Benashski, S. E., McCaffery, J. M., Goldstein, L. S.B., King, S. M. (1999). Drosophila roadblock and Chlamydomonas Lc7: A Conserved Family of Dynein-Associated Proteins Involved in Axonal Transport, Flagellar Motility, and Mitosis. JCB 146: 165-180 [Abstract] [Full Text]
Jones, M. H., Bachant, J. B., Castillo, A. R., Giddings, T. H. Jr., Winey, M. (1999). Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase. Mol. Biol. Cell 10: 2377-2391 [Abstract] [Full Text]
Gheber, L., Kuo, S. C., Hoyt, M. A. (1999). Motile Properties of the Kinesin-related Cin8p Spindle Motor Extracted from Saccharomyces cerevisiae Cells. J. Biol. Chem. 274: 9564-9572 [Abstract] [Full Text]
Manning, B. D., Barrett, J. G., Wallace, J. A., Granok, H., Snyder, M. (1999). Differential Regulation of the Kar3p Kinesin-related Protein by Two Associated Proteins, Cik1p and Vik1p. JCB 144: 1219-1233 [Abstract] [Full Text]
Lee, S., Wisniewski, J. C., Dentler, W. L., Asai, D. J. (1999). Gene Knockouts Reveal Separate Functions for Two Cytoplasmic Dyneins in Tetrahymena thermophila. Mol. Biol. Cell 10: 771-784 [Abstract] [Full Text]
Sharp, D. J., McDonald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., Scholey, J. M. (1999). The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles. JCB 144: 125-138 [Abstract] [Full Text]
Straight, A. F., Sedat, J. W., Murray, A. W. (1998). Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast. JCB 143: 687-694 [Abstract] [Full Text]
Segal, M., Clarke, D. J., Reed, S. I. (1998). Clb5-associated Kinase Activity is Required Early in the Spindle Pathway for Correct Preanaphase Nuclear Positioning in Saccharomyces cerevisiae. JCB 143: 135-145 [Abstract] [Full Text]
Karki, S., LaMonte, B., Holzbaur, E. L.F. (1998). Characterization of the p22 Subunit of Dynactin Reveals the Localization of Cytoplasmic Dynein and Dynactin to the Midbody of Dividing Cells. JCB 142: 1023-1034 [Abstract] [Full Text]
Miller, R. K., Heller, K. K., Frisèn, L., Wallack, D. L., Loayza, D., Gammie, A. E., Rose, M. D. (1998). The Kinesin-related Proteins, Kip2p and Kip3p, Function Differently in Nuclear Migration in Yeast. Mol. Biol. Cell 9: 2051-2068 [Abstract] [Full Text]
Tai, A. W., Chuang, J.-Z., Sung, C.-H. (1998). Localization of Tctex-1, a Cytoplasmic Dynein Light Chain, to the Golgi Apparatus and Evidence for Dynein Complex Heterogeneity. J. Biol. Chem. 273: 19639-19649 [Abstract] [Full Text]
Kahana, J. A., Schlenstedt, G., Evanchuk, D. M., Geiser, J. R., Hoyt, M. A., Silver, P. A. (1998). The Yeast Dynactin Complex Is Involved in Partitioning the Mitotic Spindle between Mother and Daughter Cells during Anaphase B. Mol. Biol. Cell 9: 1741-1756 [Abstract] [Full Text]
Efimov, V. P., Morris, N. R. (1998). A Screen for Dynein Synthetic Lethals in Aspergillus nidulans Identifies Spindle Assembly Checkpoint Genes and Other Genes Involved in Mitosis. Genetics 149: 101-116 [Abstract] [Full Text]
Harada, A., Takei, Y., Kanai, Y., Tanaka, Y., Nonaka, S., Hirokawa, N. (1998). Golgi Vesiculation and Lysosome Dispersion in Cells Lacking Cytoplasmic Dynein. JCB 141: 51-59 [Abstract] [Full Text]
Li, S.-H., Gutekunst, C.-A., Hersch, S. M., Li, X.-J. (1998). Interaction of Huntingtin-Associated Protein with Dynactin P150Glued. J. Neurosci. 18: 1261-1269 [Abstract] [Full Text]
Criswell, P. S., Asai, D. J. (1998). Evidence for Four Cytoplasmic Dynein Heavy Chain Isoforms in Rat Testis. Mol. Biol. Cell 9: 237-247 [Abstract] [Full Text]
Whitehead, C., Rattner, J. (1998). Expanding the role of HsEg5 within the mitotic and post-mitotic phases of the cell cycle. J. Cell Sci. 111: 2551-2561 [Abstract]
Inoue, S, Yoder, O., Turgeon, B., Aist, J. (1998). A cytoplasmic dynein required for mitotic aster formation in vivo. J. Cell Sci. 111: 2607-2614 [Abstract]
Luca, F. C., Winey, M. (1998). MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy. Mol. Biol. Cell 9: 29-46 [Abstract] [Full Text]
Hoyt, M. A., Hyman, A. A., Bahler, M. (1997). Motor proteins of the eukaryotic�cytoskeleton. Proc. Natl. Acad. Sci. USA 94: 12747-12748 [Full Text]
Shaw, S. L., Yeh, E., Maddox, P., Salmon, E.D., Bloom, K. (1997). Astral Microtubule Dynamics in Yeast: A Microtubule-based Searching Mechanism for Spindle Orientation and Nuclear Migration into the Bud. JCB 139: 985-994 [Abstract] [Full Text]
Yao, X., Anderson, K. L., Cleveland, D. W. (1997). The Microtubule-dependent Motor Centromere-associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules. JCB 139: 435-447 [Abstract] [Full Text]
DeZwaan, T. M., Ellingson, E., Pellman, D., Roof, D. M. (1997). Kinesin-related KIP3 of Saccharomyces cerevisiae Is Required for a Distinct Step in Nuclear Migration. JCB 138: 1023-1040 [Abstract] [Full Text]
Cottingham, F. R., Hoyt, M. A. (1997). Mitotic Spindle Positioning in Saccharomyces cerevisiae Is Accomplished by Antagonistically Acting Microtubule Motor Proteins. JCB 138: 1041-1053 [Abstract] [Full Text]
Carminati, J. L., Stearns, T. (1997). Microtubules Orient the Mitotic Spindle in Yeast through Dynein-dependent Interactions with the Cell Cortex. JCB 138: 629-641 [Abstract] [Full Text]
Blangy, A., Arnaud, L., Nigg, E. A. (1997). Phosphorylation by p34cdc2 Protein Kinase Regulates Binding of the Kinesin-related Motor HsEg5 to the Dynactin Subunit p150Glued. J. Biol. Chem. 272: 19418-19424 [Abstract] [Full Text]
Wheatley, S. P., Hinchcliffe, E. H., Glotzer, M., Hyman, A. A., Sluder, G., Wang, Y.-l. (1997). CDK1 Inactivation Regulates Anaphase Spindle Dynamics and Cytokinesis In Vivo. JCB 138: 385-393 [Abstract] [Full Text]
Walczak, C. E., Verma, S., Mitchison, T. J. (1997). XCTK2: A Kinesin-related Protein That Promotes Mitotic Spindle Assembly in Xenopus laevis Egg Extracts. JCB 136: 859-870 [Abstract] [Full Text]
Yang, S. S., Yeh, E., Salmon, E.D., Bloom, K. (1997). Identification of a Mid-anaphase Checkpoint in Budding Yeast. JCB 136: 345-354 [Abstract] [Full Text]
Asada, T, Kuriyama, R, Shibaoka, H (1997). TKRP125, a kinesin-related protein involved in the centrosome-independent organization of the cytokinetic apparatus in tobacco BY-2 cells. J. Cell Sci. 110: 179-189 [Abstract]
King, S. M., Dillman, J. F. III, Benashski, S. E., Lye, R. J., Patel-King, RamilaS., Pfister, K.K. (1996). The Mouse t-Complex-encoded Protein Tctex-1 Is a Light Chain of Brain Cytoplasmic Dynein. J. Biol. Chem. 271: 32281-32287 [Abstract] [Full Text]
King, S. M., Barbarese, E., Dillman, J. F. III, Patel-King, R. S., Carson, J. H., Pfister, K. K. (1996). Brain Cytoplasmic and Flagellar Outer Arm Dyneins Share a Highly Conserved Mr 8,000Light Chain. J. Biol. Chem. 271: 19358-19366 [Abstract] [Full Text]
Brown, K., Wood, K., Cleveland, D. (1996). The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A. J. Cell Sci. 109: 961-969 [Abstract]
Vallee, R.B., Vaughan, K.T., Escheverri, C.J. (1995). Targeting of Cytoplasmic Dynein to Membranous Organelles and Kinetochores via Dynactin. Cold Spring Harb Symp Quant Biol 60: 803-811 [Abstract]
Xiang, X., Osmani, A.H., Osmani, S.A., Roghi, C.H., Willins, D.A., Beckwith, S., Goldman, G., Chiu, Y., Xin, M., Liu, B., Morris, N.R. (1995). Analysis of Nuclear Migration in Aspergillus nidulans. Cold Spring Harb Symp Quant Biol 60: 813-819 [Abstract]
Gordon, D. M., Roof, D. M. (2001). Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc. Natl. Acad. Sci. USA 98: 12515-12520 [Abstract] [Full Text]