Nuclear localization of the testis determining gene product SRY

doi:10.1083/jcb.128.5.737

This Article

	Full Text (PDF, 4114K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Poulat, F.
	Articles by Berta, P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Poulat, F.
	Articles by Berta, P.


Social Bookmarking

	What's this?

ARTICLES

Nuclear localization of the testis determining gene product SRY

F Poulat, F Girard, MP Chevron, C Goze, X Rebillard, B Calas, N Lamb and P Berta
Centre de Recherche de Biochimie Macromoleculaire, CNRS/INSERM, Montpellier, France.

We have studied the expression of the human SRY protein (termed p27SRY) in two different cell lines by using specific antibodies. Confocal microscopy enabled us to localize p27SRY precisely in the nucleus in a discrete punctuate pattern. Furthermore, through microinjection experiments, we have demonstrated that the localization of the p27SRY protein into the nucleus was an event involving the NH2-terminal part of the high mobility group (HMG) domain. With the help of several synthetic peptides and various p27SRY mutants, we have characterized a bipartite basic motif in this part of the protein corresponding to a nuclear localization signal. This nuclear localization signal appears to be highly conserved in SRY box- and HMB box-containing proteins, suggesting common properties of nuclear targeting within the HMG box protein family.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Wu, J. B., Chen, K., Li, Y., Lau, Y.-F. C., Shih, J. C. (2009). Regulation of monoamine oxidase A by the SRY gene on the Y chromosome. FASEB J. 23: 4029-4038 [Abstract] [Full Text]
Gontan, C., Guttler, T., Engelen, E., Demmers, J., Fornerod, M., Grosveld, F. G., Tibboel, D., Gorlich, D., Poot, R. A., Rottier, R. J. (2009). Exportin 4 mediates a novel nuclear import pathway for Sox family transcription factors. JCB 185: 27-34 [Abstract] [Full Text]
Ek, S., Dictor, M., Jerkeman, M., Jirstrom, K., Borrebaeck, C. A. K. (2008). Nuclear expression of the non B-cell lineage Sox11 transcription factor identifies mantle cell lymphoma. Blood 111: 800-805 [Abstract] [Full Text]
Ito, I., Fukazawa, J., Yoshida, M. (2007). Post-translational Methylation of High Mobility Group Box 1 (HMGB1) Causes Its Cytoplasmic Localization in Neutrophils. J. Biol. Chem. 282: 16336-16344 [Abstract] [Full Text]
Launholt, D., Merkle, T., Houben, A., Schulz, A., Grasser, K. D. (2006). Arabidopsis Chromatin-Associated HMGA and HMGB Use Different Nuclear Targeting Signals and Display Highly Dynamic Localization within the Nucleus. Plant Cell 18: 2904-2918 [Abstract] [Full Text]
Modi, D, Shah, C, Sachdeva, G, Gadkar, S, Bhartiya, D, Puri, C (2005). Ontogeny and cellular localization of SRY transcripts in the human testes and its detection in spermatozoa. Reproduction 130: 603-613 [Abstract] [Full Text]
Sim, H., Rimmer, K., Kelly, S., Ludbrook, L. M., Clayton, A. H. A., Harley, V. R. (2005). Defective Calmodulin-Mediated Nuclear Transport of the Sex-Determining Region of the Y Chromosome (SRY) in XY Sex Reversal. Mol. Endocrinol. 19: 1884-1892 [Abstract] [Full Text]
Harley, V. R., Clarkson, M. J., Argentaro, A. (2003). The Molecular Action and Regulation of the Testis-Determining Factors, SRY (Sex-Determining Region on the Y Chromosome) and SOX9 [SRY-Related High-Mobility Group (HMG) Box 9]. Endocr. Rev. 24: 466-487 [Abstract] [Full Text]
Harley, V. R., Layfield, S., Mitchell, C. L., Forwood, J. K., John, A. P., Briggs, L. J., McDowall, S. G., Jans, D. A. (2003). Defective importin {beta} recognition and nuclear import of the sex-determining factor SRY are associated with XY sex-reversing mutations. Proc. Natl. Acad. Sci. USA 100: 7045-7050 [Abstract] [Full Text]
Hosoda, K., Imamura, A., Katoh, E., Hatta, T., Tachiki, M., Yamada, H., Mizuno, T., Yamazaki, T. (2002). Molecular Structure of the GARP Family of Plant Myb-Related DNA Binding Motifs of the Arabidopsis Response Regulators. Plant Cell 14: 2015-2029 [Abstract] [Full Text]
Ohe, K., Lalli, E., Sassone-Corsi, P. (2002). A direct role of SRY and SOX proteins in pre-mRNA splicing. Proc. Natl. Acad. Sci. USA 10.1073/pnas.022645899v1 [Abstract] [Full Text]
Li, B., Zhang, W., Chan, G., Jancso-Radek, A., Liu, S., Weiss, M. A. (2001). Human Sex Reversal Due to Impaired Nuclear Localization of SRY. A CLINICAL CORRELATION. J. Biol. Chem. 276: 46480-46484 [Abstract] [Full Text]
Yuan, X., Lu, M. L., Li, T., Balk, S. P. (2001). SRY Interacts with and Negatively Regulates Androgen Receptor Transcriptional Activity. J. Biol. Chem. 276: 46647-46654 [Abstract] [Full Text]
Ukiyama, E., Jancso-Radek, A., Li, B., Milos, L., Zhang, W., Phillips, N. B., Morikawa, N., King, C.-Y., Chan, G., Haqq, C. M., Radek, J. T., Poulat, F., Donahoe, P. K., Weiss, M. A. (2001). SRY and Architectural Gene Regulation: The Kinetic Stability of a Bent Protein-DNA Complex Can Regulate Its Transcriptional Potency. Mol. Endocrinol. 15: 363-377 [Abstract] [Full Text]
de Santa Barbara, P., Méjean, C., Moniot, B., Malclès, M.-H., Berta, P., Boizet-Bonhoure, B. (2001). Steroidogenic Factor-1 Contributes to the Cyclic-Adenosine Monophosphate Down-Regulation of Human SRY Gene Expression. Biol. Reprod. 64: 775-783 [Abstract] [Full Text]
Beranger, F., Mejean, C., Moniot, B., Berta, P., Vandromme, M. (2000). Muscle Differentiation Is Antagonized by SOX15, a New Member of the SOX Protein Family. J. Biol. Chem. 275: 16103-16109 [Abstract] [Full Text]
Kuhlbrodt, K., Schmidt, C., Sock, E., Pingault, V., Bondurand, N., Goossens, M., Wegner, M. (1998). Functional Analysis of Sox10 Mutations Found in Human Waardenburg-Hirschsprung Patients. J. Biol. Chem. 273: 23033-23038 [Abstract] [Full Text]
Desclozeaux, M., Poulat, F., Barbara, P. d. S., Capony, J.-P., Turowski, P., Jay, P., Mejean, C., Moniot, B., Boizet, B., Berta, P. (1998). Phosphorylation of an N-terminal Motif Enhances DNA-binding Activity of the Human SRY Protein. J. Biol. Chem. 273: 7988-7995 [Abstract] [Full Text]
Yen, Y.-M., Wong, B., Johnson, R. C. (1998). Determinants of DNA Binding and Bending by the Saccharomyces cerevisiae High Mobility Group Protein NHP6A That Are Important for Its Biological Activities. ROLE OF THE UNIQUE N TERMINUS AND PUTATIVE INTERCALATING METHIONINE. J. Biol. Chem. 273: 4424-4435 [Abstract] [Full Text]
Sudbeck, P., Scherer, G. (1997). Two Independent Nuclear Localization Signals Are Present in the DNA-binding High-mobility Group Domains of SRY and SOX9. J. Biol. Chem. 272: 27848-27852 [Abstract] [Full Text]
Poulat, F., Barbara, P. d. S., Desclozeaux, M., Soullier, S., Moniot, B., Bonneaud, N., Boizet, B., Berta, P. (1997). The Human Testis Determining Factor SRY Binds a Nuclear Factor Containing PDZ Protein Interaction Domains. J. Biol. Chem. 272: 7167-7172 [Abstract] [Full Text]
Kohler, A, Schmidt-Zachmann, M., Franke, W. (1997). AND-1, a natural chimeric DNA-binding protein, combines an HMG-box with regulatory WD-repeats. J. Cell Sci. 110: 1051-1062 [Abstract]
Sock, E., Enderich, J., Rosenfeld, M. G., Wegner, M. (1996). Identification of the Nuclear Localization Signal of the POU Domain Protein Tst-1/Oct6. J. Biol. Chem. 271: 17512-17518 [Abstract] [Full Text]
Hossain, A., Saunders, G. F. (2001). The Human Sex-determining Gene SRY Is a Direct Target of WT1. J. Biol. Chem. 276: 16817-16823 [Abstract] [Full Text]
Ohe, K., Lalli, E., Sassone-Corsi, P. (2002). A direct role of SRY and SOX proteins in pre-mRNA splicing. Proc. Natl. Acad. Sci. USA 99: 1146-1151 [Abstract] [Full Text]