CEP3 encodes a centromere protein of Saccharomyces cerevisiae

doi:10.1083/jcb.128.5.749

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CEP3 encodes a centromere protein of Saccharomyces cerevisiae

AV Strunnikov, J Kingsbury and D Koshland
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.

We have designed a screen to identify mutants specifically affecting kinetochore function in the yeast Saccharomyces cerevisiae. The selection procedure was based on the generation of "synthetic acentric" minichromosomes. "Synthetic acentric" minichromosomes contain a centromere locus, but lack centromere activity due to combination of mutations in centromere DNA and in a chromosomal gene (CEP) encoding a putative centromere protein. Ten conditional lethal cep mutants were isolated, seven were found to be alleles of NDC10 (CEP2) encoding the 110-kD protein of yeast kinetochore. Three mutants defined a novel essential gene CEP3. The CEP3 product (Cep3p) is a 71-kD protein with a potential DNA-binding domain (binuclear Zn-cluster). At nonpermissive temperature the cep3 cells arrest with an undivided nucleus and a short mitotic spindle. At permissive temperature the cep3 cells are unable to support segregation of minichromosomes with mutations in the central part of element III of yeast centromere DNA. These minichromosomes, when isolated from cep3 cultures, fail to bind bovine microtubules in vitro. The sum of genetic, cytological and biochemical data lead us to suggest that the Cep3 protein is a DNA-binding component of yeast centromere. Molecular mass and sequence comparison confirm that Cep3p is the p64 component of centromere DNA binding complex Cbf3 (Lechner, 1994).
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Rodrigo-Brenni, M. C., Thomas, S., Bouck, D. C., Kaplan, K. B. (2004). Sgt1p and Skp1p Modulate the Assembly and Turnover of CBF3 Complexes Required for Proper Kinetochore Function. Mol. Biol. Cell 15: 3366-3378 [Abstract] [Full Text]
Gillett, E. S., Espelin, C. W., Sorger, P. K. (2004). Spindle checkpoint proteins and chromosome-microtubule attachment in budding yeast. JCB 164: 535-546 [Abstract] [Full Text]
De Wulf, P., McAinsh, A. D., Sorger, P. K. (2003). Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev. 17: 2902-2921 [Abstract] [Full Text]
Espelin, C. W., Simons, K. T., Harrison, S. C., Sorger, P. K. (2003). Binding of the Essential Saccharomyces cerevisiae Kinetochore Protein Ndc10p to CDEII. Mol. Biol. Cell 14: 4557-4568 [Abstract] [Full Text]
Pot, I., Measday, V., Snydsman, B., Cagney, G., Fields, S., Davis, T. N., Muller, E. G.D., Hieter, P. (2003). Chl4p and Iml3p Are Two New Members of the Budding Yeast Outer Kinetochore. Mol. Biol. Cell 14: 460-476 [Abstract] [Full Text]
Mehta, S., Yang, X. M., Chan, C. S., Dobson, M. J., Jayaram, M., Velmurugan, S. (2002). The 2 micron plasmid purloins the yeast cohesin complex: a mechanism for coupling plasmid partitioning and chromosome segregation?. JCB 158: 625-637 [Abstract] [Full Text]
Abruzzi, K. C., Magendantz, M., Solomon, F. (2002). An {alpha}-Tubulin Mutant Demonstrates Distinguishable Functions Among the Spindle Assembly Checkpoint Genes in Saccharomyces cerevisiae. Genetics 161: 983-994 [Abstract] [Full Text]
Li, Y., Bachant, J., Alcasabas, A. A., Wang, Y., Qin, J., Elledge, S. J. (2002). The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev. 16: 183-197 [Abstract] [Full Text]
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Gardner, R. D., Poddar, A., Yellman, C., Tavormina, P. A., Monteagudo, M. C., Burke, D. J. (2001). The Spindle Checkpoint of the Yeast Saccharomyces cerevisiae Requires Kinetochore Function and Maps to the CBF3 Domain. Genetics 157: 1493-1502 [Abstract] [Full Text]
Wigge, P. A., Kilmartin, J. V. (2001). The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation. JCB 152: 349-360 [Abstract] [Full Text]
Keith, K. C., Fitzgerald-Hayes, M. (2000). CSE4 Genetically Interacts With the Saccharomyces cerevisiae Centromere DNA Elements CDE I and CDE II but Not CDE III: Implications for the Path of the Centromere DNA Around a Cse4p Variant Nucleosome. Genetics 156: 973-981 [Abstract] [Full Text]
Lin, H., Choi, J. H., Hasek, J., DeLillo, N., Lou, W., Vancura, A. (2000). Phospholipase C Is Involved in Kinetochore Function in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 3597-3607 [Abstract] [Full Text]
Lavoie, B. D., Tuffo, K. M., Oh, S., Koshland, D., Holm, C. (2000). Mitotic Chromosome Condensation Requires Brn1p, the Yeast Homologue of Barren. Mol. Biol. Cell 11: 1293-1304 [Abstract] [Full Text]
Yoon, H.-J., Carbon, J. (1999). Participation of Bir1p, a member of the inhibitor of apoptosis family, in yeast chromosome segregation events. Proc. Natl. Acad. Sci. USA 96: 13208-13213 [Abstract] [Full Text]
Russell, I. D., Grancell, A. S., Sorger, P. K. (1999). The Unstable F-box Protein p58-Ctf13 Forms the Structural Core of the CBF3 Kinetochore Complex. JCB 145: 933-950 [Abstract] [Full Text]
Ortiz, J., Stemmann, O., Rank, S., Lechner, J. (1999). A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev. 13: 1140-1155 [Abstract] [Full Text]
Hyland, K. M., Kingsbury, J., Koshland, D., Hieter, P. (1999). Ctf19p: A Novel Kinetochore Protein in Saccharomyces cerevisiae and a Potential Link between the Kinetochore and Mitotic Spindle. JCB 145: 15-28 [Abstract] [Full Text]
Sassoon, I., Severin, F. F., Andrews, P. D., Taba, M.-R., Kaplan, K. B., Ashford, A. J., Stark, M. J.R., Sorger, P. K., Hyman, A. A. (1999). Regulation of Saccharomyces cerevisiae kinetochores by the type 1�phosphatase Glc7p. Genes Dev. 13: 545-555 [Abstract] [Full Text]
Dahlseid, J. N., Puziss, J., Shirley, R. L., Atkin, A. L., Hieter, P., Culbertson, M. R. (1998). Accumulation of mRNA Coding for the Ctf13p Kinetochore Subunit of Saccharomyces cerevisiae Depends on the Same Factors That Promote Rapid Decay of Nonsense mRNAs. Genetics 150: 1019-1035 [Abstract] [Full Text]
Wigge, P. A., Jensen, O. N., Holmes, S., Soues, S., Mann, M., Kilmartin, J. V. (1998). Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry. JCB 141: 967-977 [Abstract] [Full Text]
Baker, R. E., Harris, K., Zhang, K. (1998). Mutations Synthetically Lethal with cep1 Target S. cerevisiae Kinetochore Components. Genetics 149: 73-85 [Abstract] [Full Text]
Tavormina, P. A., Burke, D. J. (1998). Cell Cycle Arrest in cdc20 Mutants of Saccharomyces cerevisiae Is Independent of Ndc10p and Kinetochore Function but Requires a Subset of Spindle Checkpoint Genes. Genetics 148: 1701-1713 [Abstract] [Full Text]
Espelin, C. W., Kaplan, K. B., Sorger, P. K. (1997). Probing the Architecture of a Simple Kinetochore Using DNA-Protein Crosslinking. JCB 139: 1383-1396 [Abstract] [Full Text]
Meluh, P. B., Koshland, D. (1997). Budding yeast centromere composition and assembly as revealed by in vivo�cross-linking. Genes Dev. 11: 3401-3412 [Abstract] [Full Text]
Ekwall, K, Nimmo, E., Javerzat, J., Borgstrom, B, Egel, R, Cranston, G, Allshire, R (1996). Mutations in the fission yeast silencing factors clr4+ and rik1+ disrupt the localisation of the chromo domain protein Swi6p and impair centromere function. J. Cell Sci. 109: 2637-2648 [Abstract]
Pluta, A. F., Mackay, A. M., Ainsztein, A. M., Goldberg, I. G., Earnshaw, W. C. (1995). The Centromere: Hub of Chromosomal Activities. Science 270: 1591-1594 [Abstract]