The membrane protein CD9/DRAP 27 potentiates the juxtacrine growth factor activity of the membrane-anchored heparin-binding EGF-like growth factor

doi:10.1083/jcb.128.5.929

This Article

	Full Text (PDF, 1312K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Higashiyama, S.
	Articles by Mekada, E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Higashiyama, S.
	Articles by Mekada, E.


Social Bookmarking

	What's this?

ARTICLES

The membrane protein CD9/DRAP 27 potentiates the juxtacrine growth factor activity of the membrane-anchored heparin-binding EGF-like growth factor

S Higashiyama, R Iwamoto, K Goishi, G Raab, N Taniguchi, M Klagsbrun and E Mekada
Department of Biochemistry, Osaka University Medical School, Japan.

The membrane-anchored heparin-binding EGF-like growth factor precursor (proHB-EGF)/diphtheria toxin receptor (DTR) belongs to a class of transmembrane growth factors and physically associates with CD9/DRAP27 which is also a transmembrane protein. To evaluate the biological activities of proHB-EGF/DTR as a juxtacrine growth factor and the biological significance of its association with CD9/DRAP27, the mitogenic activity of proHB-EGF/DTR was analyzed using stable transfectants of mouse L cells expressing both human proHB-EGF/DTR and monkey CD9/DRAP27, or either one alone. Juxtacrine activity was assayed by measuring the ability of cells in co-culture to stimulate DNA synthesis in an EGF receptor ligand dependent cell line, EP170.7. LH-2 cells expressing human proHB-EGF/DTR stimulated EP170.7 cell growth moderately. However, LCH-1 cells, a stable co-transfectant expressing both human proHB-EGF/DTR and monkey CD9/DRAP27 cDNAs, dramatically unregulated the juxtacrine growth factor activity of proHB-EGF/DTR approximately 25 times over that of LH-2 cells even though both cell types expressed similar levels of proHB-EGF/DTR on the cell surface. Anti-CD9/DRAP27 antibodies which were not able to neutralize the mitogenic activity of soluble HB-EGF suppressed LCH-1 cell juxtacrine growth activity to the same extent as did anti-HB-EGF neutralizing antibodies and CRM 197, specific inhibitors of human HG-EGF. These findings suggest that optimal expression of the juxtacrine growth activity of proHB-EGF/DTR requires co-expression of CD9/DRAP27. These studies also indicate that growth factor potentiation effects which have been observed previously for soluble growth factors also occurs at the level of cell surface associated growth factors.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Mizushima, H., Wang, X., Miyamoto, S., Mekada, E. (2009). Integrin signal masks growth-promotion activity of HB-EGF in monolayer cell cultures. J. Cell Sci. 122: 4277-4286 [Abstract] [Full Text]
Zhang, F., Kotha, J., Jennings, L. K., Zhang, X. A. (2009). Tetraspanins and vascular functions. Cardiovasc Res 83: 7-15 [Abstract] [Full Text]
Isokane, M., Hieda, M., Hirakawa, S., Shudou, M., Nakashiro, K., Hashimoto, K., Hamakawa, H., Higashiyama, S. (2008). Plasma-membrane-anchored growth factor pro-amphiregulin binds A-type lamin and regulates global transcription. J. Cell Sci. 121: 3608-3618 [Abstract] [Full Text]
Yagi, H., Yotsumoto, F., Miyamoto, S. (2008). Heparin-binding epidermal growth factor-like growth factor promotes transcoelomic metastasis in ovarian cancer through epithelial-mesenchymal transition. Molecular Cancer Therapeutics 7: 3441-3451 [Abstract] [Full Text]
Singh, A. B., Sugimoto, K., Harris, R. C. (2007). Juxtacrine Activation of Epidermal Growth Factor (EGF) Receptor by Membrane-anchored Heparin-binding EGF-like Growth Factor Protects Epithelial Cells from Anoikis While Maintaining an Epithelial Phenotype. J. Biol. Chem. 282: 32890-32901 [Abstract] [Full Text]
Kovalenko, O. V., Yang, X. H., Hemler, M. E. (2007). A Novel Cysteine Cross-linking Method Reveals a Direct Association between Claudin-1 and Tetraspanin CD9. Mol. Cell. Proteomics 6: 1855-1867 [Abstract] [Full Text]
Kinugasa, Y., Hieda, M., Hori, M., Higashiyama, S. (2007). The Carboxyl-terminal Fragment of Pro-HB-EGF Reverses Bcl6-mediated Gene Repression. J. Biol. Chem. 282: 14797-14806 [Abstract] [Full Text]
Chen, J., Chen, J.-K., Falck, J. R., Anjaiah, S., Capdevila, J. H., Harris, R. C. (2007). Mitogenic Activity and Signaling Mechanism of 2-(14,15- Epoxyeicosatrienoyl)Glycerol, a Novel Cytochrome P450 Arachidonate Metabolite. Mol. Cell. Biol. 27: 3023-3034 [Abstract] [Full Text]
Yin, J., Xu, K., Zhang, J., Kumar, A., Yu, F.-S. X. (2007). Wound-induced ATP release and EGF receptor activation in epithelial cells. J. Cell Sci. 120: 815-825 [Abstract] [Full Text]
Montero, J. C., Rodriguez-Barrueco, R., Yuste, L., Juanes, P. P., Borges, J., Esparis-Ogando, A., Pandiella, A. (2007). The Extracellular Linker of pro-Neuregulin-{alpha}2c Is Required for Efficient Sorting and Juxtacrine Function. Mol. Biol. Cell 18: 380-393 [Abstract] [Full Text]
Willmarth, N. E., Ethier, S. P. (2006). Autocrine and Juxtacrine Effects of Amphiregulin on the Proliferative, Invasive, and Migratory Properties of Normal and Neoplastic Human Mammary Epithelial Cells. J. Biol. Chem. 281: 37728-37737 [Abstract] [Full Text]
Furukawa, N., Saito, M., Hakoshima, T., Kohno, K. (2006). A Diphtheria Toxin Receptor Deficient in Epidermal Growth Factor-Like Biological Activity. J Biochem 140: 831-841 [Abstract] [Full Text]
Xu, K.-P., Yin, J., Yu, F.-S. X. (2006). SRC-family tyrosine kinases in wound- and ligand-induced epidermal growth factor receptor activation in human corneal epithelial cells.. IOVS 47: 2832-2839 [Abstract] [Full Text]
Yang, X. H., Kovalenko, O. V., Kolesnikova, T. V., Andzelm, M. M., Rubinstein, E., Strominger, J. L., Hemler, M. E. (2006). Contrasting Effects of EWI Proteins, Integrins, and Protein Palmitoylation on Cell Surface CD9 Organization. J. Biol. Chem. 281: 12976-12985 [Abstract] [Full Text]
Mai, W., Chen, D., Ding, T., Kim, I., Park, S., Cho, S.-y., Chu, J. S.F., Liang, D., Wang, N., Wu, D., Li, S., Zhao, P., Zent, R., Wu, G. (2005). Inhibition of Pkhd1 Impairs Tubulomorphogenesis of Cultured IMCD Cells. Mol. Biol. Cell 16: 4398-4409 [Abstract] [Full Text]
Yoshioka, J., Prince, R. N., Huang, H., Perkins, S. B., Cruz, F. U., MacGillivray, C., Lauffenburger, D. A., Lee, R. T. (2005). Cardiomyocyte hypertrophy and degradation of connexin43 through spatially restricted autocrine/paracrine heparin-binding EGF. Proc. Natl. Acad. Sci. USA 102: 10622-10627 [Abstract] [Full Text]
Peters, D. G., Kudla, D. M., DeLoia, J. A., Chu, T. J., Fairfull, L., Edwards, R. P., Ferrell, R. E. (2005). Comparative Gene Expression Analysis of Ovarian Carcinoma and Normal Ovarian Epithelium by Serial Analysis of Gene Expression. Cancer Epidemiol. Biomarkers Prev. 14: 1717-1723 [Abstract] [Full Text]
Dong, J., Opresko, L. K., Chrisler, W., Orr, G., Quesenberry, R. D., Lauffenburger, D. A., Wiley, H. S. (2005). The Membrane-anchoring Domain of Epidermal Growth Factor Receptor Ligands Dictates Their Ability to Operate in Juxtacrine Mode. Mol. Biol. Cell 16: 2984-2998 [Abstract] [Full Text]
Barreiro, O., Yanez-Mo, M., Sala-Valdes, M., Gutierrez-Lopez, M. D., Ovalle, S., Higginbottom, A., Monk, P. N., Cabanas, C., Sanchez-Madrid, F. (2005). Endothelial tetraspanin microdomains regulate leukocyte firm adhesion during extravasation. Blood 105: 2852-2861 [Abstract] [Full Text]
Sanderson, M. P., Erickson, S. N., Gough, P. J., Garton, K. J., Wille, P. T., Raines, E. W., Dunbar, A. J., Dempsey, P. J. (2005). ADAM10 Mediates Ectodomain Shedding of the Betacellulin Precursor Activated by p-Aminophenylmercuric Acetate and Extracellular Calcium Influx. J. Biol. Chem. 280: 1826-1837 [Abstract] [Full Text]
Yang, X., Kovalenko, O. V., Tang, W., Claas, C., Stipp, C. S., Hemler, M. E. (2004). Palmitoylation supports assembly and function of integrin-tetraspanin complexes. JCB 167: 1231-1240 [Abstract] [Full Text]
Takazaki, R., Shishido, Y., Iwamoto, R., Mekada, E. (2004). Suppression of the Biological Activities of the Epidermal Growth Factor (EGF)-like Domain by the Heparin-binding Domain of Heparin-binding EGF-like Growth Factor. J. Biol. Chem. 279: 47335-47343 [Abstract] [Full Text]
Aline, F., Bout, D., Amigorena, S., Roingeard, P., Dimier-Poisson, I. (2004). Toxoplasma gondii Antigen-Pulsed-Dendritic Cell-Derived Exosomes Induce a Protective Immune Response against T. gondii Infection. Infect. Immun. 72: 4127-4137 [Abstract] [Full Text]
Murayama, Y., Miyagawa, J.-i., Oritani, K., Yoshida, H., Yamamoto, K., Kishida, O., Miyazaki, T., Tsutsui, S., Kiyohara, T., Miyazaki, Y., Higashiyama, S., Matsuzawa, Y., Shinomura, Y. (2004). CD9-mediated activation of the p46 Shc isoform leads to apoptosis in cancer cells. J. Cell Sci. 117: 3379-3388 [Abstract] [Full Text]
Little, K. D., Hemler, M. E., Stipp, C. S. (2004). Dynamic Regulation of a GPCR-Tetraspanin-G Protein Complex on Intact Cells: Central Role of CD81 in Facilitating GPR56-G{alpha}q/11 Association. Mol. Biol. Cell 15: 2375-2387 [Abstract] [Full Text]
Singh, A. B., Tsukada, T., Zent, R., Harris, R. C. (2004). Membrane-associated HB-EGF modulates HGF-induced cellular responses in MDCK cells. J. Cell Sci. 117: 1365-1379 [Abstract] [Full Text]
Xu, K.-P., Ding, Y., Ling, J., Dong, Z., Yu, F.-S. X. (2004). Wound-Induced HB-EGF Ectodomain Shedding and EGFR Activation in Corneal Epithelial Cells. IOVS 45: 813-820 [Abstract] [Full Text]
Ishibashi, T., Ding, L., Ikenaka, K., Inoue, Y., Miyado, K., Mekada, E., Baba, H. (2004). Tetraspanin Protein CD9 Is a Novel Paranodal Component Regulating Paranodal Junctional Formation. J. Neurosci. 24: 96-102 [Abstract] [Full Text]
Le Gall, S. M., Auger, R., Dreux, C., Mauduit, P. (2003). Regulated Cell Surface Pro-EGF Ectodomain Shedding Is a Zinc Metalloprotease-dependent Process. J. Biol. Chem. 278: 45255-45268 [Abstract] [Full Text]
Liu, J., Rich, C. B., Buczek-Thomas, J. A., Nugent, M. A., Panchenko, M. P., Foster, J. A. (2003). Heparin-binding EGF-like growth factor regulates elastin and FGF-2 expression in pulmonary fibroblasts. Am. J. Physiol. Lung Cell. Mol. Physiol. 285: L1106-L1115 [Abstract] [Full Text]
Takenobu, H., Yamazaki, A., Hirata, M., Umata, T., Mekada, E. (2003). The Stress- and Inflammatory Cytokine-induced Ectodomain Shedding of Heparin-binding Epidermal Growth Factor-like Growth Factor Is Mediated by p38 MAPK, Distinct from the 12-O-Tetradecanoylphorbol-13-acetate- and Lysophosphatidic Acid-induced Signaling Cascades. J. Biol. Chem. 278: 17255-17262 [Abstract] [Full Text]
Iwamoto, R., Yamazaki, S., Asakura, M., Takashima, S., Hasuwa, H., Miyado, K., Adachi, S., Kitakaze, M., Hashimoto, K., Raab, G., Nanba, D., Higashiyama, S., Hori, M., Klagsbrun, M., Mekada, E. (2003). Heparin-binding EGF-like growth factor and ErbB signaling is essential for heart function. Proc. Natl. Acad. Sci. USA 100: 3221-3226 [Abstract] [Full Text]
Nolan, T. M., DiGirolamo, N., Sachdev, N. H., Hampartzoumian, T., Coroneo, M. T., Wakefield, D. (2003). The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium. Am. J. Pathol. 162: 567-574 [Abstract] [Full Text]
Pan, B., Sengoku, K., Goishi, K., Takuma, N., Yamashita, T., Wada, K., Ishikawa, M. (2002). The soluble and membrane-anchored forms of heparin-binding epidermal growth factor-like growth factor appear to play opposing roles in the survival and apoptosis of human luteinized granulosa cells. Mol Hum Reprod 8: 734-741 [Abstract] [Full Text]
Stavreus-Evers, A., Aghajanova, L., Brismar, H., Eriksson, H., Landgren, B.-M., Hovatta, O. (2002). Co-existence of heparin-binding epidermal growth factor-like growth factor and pinopodes in human endometrium at the time of implantation. Mol Hum Reprod 8: 765-769 [Abstract] [Full Text]
Dempsey, P. J., Garton, K., Raines, E. W. (2002). Emerging Roles of TACE as a Key Protease in ErbB Ligand Shedding. Mol. Interv. 2: 136-141 [Abstract] [Full Text]
Anzai, N., Lee, Y., Youn, B.-S., Fukuda, S., Kim, Y.-J., Mantel, C., Akashi, M., Broxmeyer, H. E. (2002). c-kit associated with the transmembrane 4 superfamily proteins constitutes a functionally distinct subunit in human hematopoietic progenitors. Blood 99: 4413-4421 [Abstract] [Full Text]
Cha, J.-H., Brooke, J. S., Chang, M. Y., Eidels, L. (2002). Receptor-Based Antidote for Diphtheria. Infect. Immun. 70: 2344-2350 [Abstract] [Full Text]
Suo, Z., Risberg, B., Karlsson, M. G., Villman, K., Skovlund, E., Nesland, J. M. (2002). The Expression of EGFR Family Ligands in Breast Carcinomas. INT J SURG PATHOL 10: 91-99 [Abstract]
Thery, C., Boussac, M., Veron, P., Ricciardi-Castagnoli, P., Raposo, G., Garin, J., Amigorena, S. (2001). Proteomic Analysis of Dendritic Cell-Derived Exosomes: A Secreted Subcellular Compartment Distinct from Apoptotic Vesicles. J. Immunol. 166: 7309-7318 [Abstract] [Full Text]
TAKEMURA, T., HINO, S., KUWAJIMA, H., YANAGIDA, H., OKADA, M., NAGATA, M., SASAKI, S., BARASCH, J., HARRIS, R. C., YOSHIOKA, K. (2001). Induction of Collecting Duct Morphogenesis In Vitro by Heparin-Binding Epidermal Growth Factor-Like Growth Factor. J. Am. Soc. Nephrol. 12: 964-972 [Abstract] [Full Text]
Nakamura, Y., Handa, K., Iwamoto, R., Tsukamoto, T., Takahasi, M., Mekada, E. (2001). Immunohistochemical Distribution of CD9, Heparin Binding Epidermal Growth Factor-like Growth Factor, and Integrin {{alpha}}3{beta}1 in Normal Human Tissues. J. Histochem. Cytochem. 49: 439-444 [Abstract] [Full Text]
Tokumaru, S., Higashiyama, S., Endo, T., Nakagawa, T., Miyagawa, J.-i., Yamamori, K., Hanakawa, Y., Ohmoto, H., Yoshino, K., Shirakata, Y., Matsuzawa, Y., Hashimoto, K., Taniguchi, N. (2000). Ectodomain Shedding of Epidermal Growth Factor Receptor Ligands Is Required for Keratinocyte Migration in Cutaneous Wound Healing. JCB 151: 209-220 [Abstract] [Full Text]
Sheikh-Hamad, D., Youker, K., Truong, L. D., Nielsen, S., Entman, M. L. (2000). Osmotically relevant membrane signaling complex: association between HB-EGF, beta 1-integrin, and CD9 in mTAL. Am. J. Physiol. Cell Physiol. 279: C136-C146 [Abstract] [Full Text]
Nishida, M., Miyagawa, J.-i., Yamashita, S., Higashiyama, S., Nakata, A., Ouchi, N., Tamura, R., Yamamori, K., Kihara, S., Taniguchi, N., Matsuzawa, Y. (2000). Localization of CD9, an Enhancer Protein for Proheparin-Binding Epidermal Growth Factor-Like Growth Factor, in Human Atherosclerotic Plaques : Possible Involvement of Juxtacrine Growth Mechanism on Smooth Muscle Cell Proliferation. Arterioscler. Thromb. Vasc. Bio. 20: 1236-1243 [Abstract] [Full Text]
Cha, J.-H., Brooke, J. S., Ivey, K. N., Eidels, L. (2000). Cell Surface Monkey CD9 Antigen Is a Coreceptor That Increases Diphtheria Toxin Sensitivity and Diphtheria Toxin Receptor Affinity. J. Biol. Chem. 275: 6901-6907 [Abstract] [Full Text]
Shi, W., Fan, H., Shum, L., Derynck, R. (2000). The Tetraspanin Cd9 Associates with Transmembrane TGF-{alpha} and Regulates TGF-{alpha}-Induced Egf Receptor Activation and Cell Proliferation. JCB 148: 591-602 [Abstract] [Full Text]
Miyado, K., Yamada, G., Yamada, S., Hasuwa, H., Nakamura, Y., Ryu, F., Suzuki, K., Kosai, K., Inoue, K., Ogura, A., Okabe, M., Mekada, E. (2000). Requirement of CD9 on the Egg Plasma Membrane for Fertilization. Science 287: 321-324 [Abstract] [Full Text]
Dong, J., Wiley, H. S. (2000). Trafficking and Proteolytic Release of Epidermal Growth Factor Receptor Ligands Are Modulated by Their Membrane-anchoring Domains. J. Biol. Chem. 275: 557-564 [Abstract] [Full Text]
Yu, X, Miyamoto, S, Mekada, E (2000). Integrin alpha 2 beta 1-dependent EGF receptor activation at cell-cell contact sites. J. Cell Sci. 113: 2139-2147 [Abstract]
Thery, C., Regnault, A., Garin, J., Wolfers, J., Zitvogel, L., Ricciardi-Castagnoli, P., Raposo, G., Amigorena, S. (1999). Molecular Characterization of Dendritic Cell-Derived Exosomes: Selective Accumulation of the Heat Shock Protein Hsc73. JCB 147: 599-610 [Abstract] [Full Text]
Gechtman, Z.'e., Alonso, J. L., Raab, G., Ingber, D. E., Klagsbrun, M. (1999). The Shedding of Membrane-anchored Heparin-binding Epidermal-like Growth Factor Is Regulated by the Raf/Mitogen-activated Protein Kinase Cascade and by Cell Adhesion and Spreading. J. Biol. Chem. 274: 28828-28835 [Abstract] [Full Text]
Iwamoto, R., Handa, K., Mekada, E. (1999). Contact-dependent Growth Inhibition and Apoptosis of Epidermal Growth Factor (EGF) Receptor-expressing Cells by the Membrane-anchored Form of Heparin-binding EGF-like Growth Factor. J. Biol. Chem. 274: 25906-25912 [Abstract] [Full Text]
Leach, R. E., Khalifa, R., Ramirez, N. D., Das, S. K., Wang, J., Dey, S. K., Romero, R., Armant, D. R. (1999). Multiple Roles for Heparin-Binding Epidermal Growth Factor-Like Growth Factor Are Suggested by Its Cell-Specific Expression during the Human Endometrial Cycle and Early Placentation. J. Clin. Endocrinol. Metab. 84: 3355-3363 [Abstract] [Full Text]
Berditchevski, F., Odintsova, E. (1999). Characterization of Integrin-Tetraspanin Adhesion Complexes: Role of Tetraspanins in Integrin Signaling. JCB 146: 477-492 [Abstract] [Full Text]
Dong, J., Opresko, L. K., Dempsey, P. J., Lauffenburger, D. A., Coffey, R. J., Wiley, H. S. (1999). Metalloprotease-mediated ligand release regulates autocrine signaling through the epidermal growth factor receptor. Proc. Natl. Acad. Sci. USA 96: 6235-6240 [Abstract] [Full Text]
Aoyama, K., Oritani, K., Yokota, T., Ishikawa, J., Nishiura, T., Miyake, K., Kanakura, Y., Tomiyama, Y., Kincade, P. W., Matsuzawa, Y. (1999). Stromal Cell CD9 Regulates Differentiation of Hematopoietic Stem/Progenitor Cells. Blood 93: 2586-2594 [Abstract] [Full Text]
Garach-Jehoshua, O., Ravid, A., Liberman, U. A., Koren, R. (1999). 1,25-Dihydroxyvitamin D3 Increases the Growth-Promoting Activity of Autocrine Epidermal Growth Factor Receptor Ligands in Keratinocytes. Endocrinology 140: 713-721 [Abstract] [Full Text]
Lauffenburger, D. A., Oehrtman, G. T., Walker, L., Wiley, H. S. (1998). Real-time quantitative measurement of autocrine ligand binding indicates that autocrine loops are spatially localized. Proc. Natl. Acad. Sci. USA 95: 15368-15373 [Abstract] [Full Text]
Wiley, H. S., Woolf, M. F., Opresko, L. K., Burke, P. M., Will, B., Morgan, J. R., Lauffenburger, D. A. (1998). Removal of the Membrane-anchoring Domain of Epidermal Growth Factor Leads to Intracrine Signaling and Disruption of Mammary Epithelial Cell Organization. JCB 143: 1317-1328 [Abstract] [Full Text]
Hammond, C., Denzin, L. K., Pan, M., Griffith, J. M., Geuze, H. J., Cresswell, P. (1998). The Tetraspan Protein CD82 Is a Resident of MHC Class II Compartments Where It Associates with HLA-DR, -DM, and -DO Molecules. J. Immunol. 161: 3282-3291 [Abstract] [Full Text]
Nishi, N., Matsushita, O., Yuube, K., Miyanaka, H., Okabe, A., Wada, F. (1998). Collagen-binding growth factors: Production and characterization of functional fusion proteins having a collagen-binding domain. Proc. Natl. Acad. Sci. USA 95: 7018-7023 [Abstract] [Full Text]
Fujino, T., Hasebe, N., Fujita, M., Takeuchi, K., Kawabe, J.-i., Tobise, K., Higashiyama, S., Taniguchi, N., Kikuchi, K. (1998). Enhanced expression of heparin-binding EGF-like growth factor and its receptor in hypertrophied left ventricle of spontaneously hypertensive rats. Cardiovasc Res 38: 365-374 [Abstract] [Full Text]
Suzuki, M., Raab, G., Moses, M. A., Fernandez, C. A., Klagsbrun, M. (1997). Matrix Metalloproteinase-3 Releases Active Heparin-binding EGF-like Growth Factor by Cleavage at a Specific Juxtamembrane Site. J. Biol. Chem. 272: 31730-31737 [Abstract] [Full Text]
Takemura, T., Kondo, S., Homma, T., Sakai, M., Harris, R. C. (1997). The Membrane-bound Form of Heparin-binding Epidermal Growth Factor-like Growth Factor Promotes Survival of Cultured Renal Epithelial Cells. J. Biol. Chem. 272: 31036-31042 [Abstract] [Full Text]
Mitamura, T., Umata, T., Nakano, F., Shishido, Y., Toyoda, T., Itai, A., Kimura, H., Mekada, E. (1997). Structure-Function Analysis of the Diphtheria Toxin Receptor Toxin Binding Site by Site-directed Mutagenesis. J. Biol. Chem. 272: 27084-27090 [Abstract] [Full Text]
Miyoshi, E., Higashiyama, S., Nakagawa, T., Hayashi, N., Taniguchi, N. (1997). Membrane-anchored Heparin-binding Epidermal Growth Factor-like Growth Factor Acts as a Tumor Survival Factor in a Hepatoma Cell Line. J. Biol. Chem. 272: 14349-14355 [Abstract] [Full Text]
Nishi, E., Kume, N., Ochi, H., Moriwaki, H., Wakatsuki, Y., Higashiyama, S., Taniguchi, N., Kita, T. (1997). Lysophosphatidylcholine Increases Expression of Heparin-Binding Epidermal Growth Factor�Like Growth Factor in Human T Lymphocytes. Circ. Res. 80: 638-644 [Abstract] [Full Text]
Nakagawa, T., Higashiyama, S., Mitamura, T., Mekada, E., Taniguchi, N. (1996). Amino-terminal Processing of Cell Surface Heparin-binding Epidermal Growth Factor-like Growth Factor Up-regulates Its Juxtacrine but Not Its Paracrine Growth Factor Activity. J. Biol. Chem. 271: 30858-30863 [Abstract] [Full Text]
Shum, L., Turck, C. W., Derynck, R. (1996). Cysteines 153 and 154 of Transmembrane Transforming Growth Factor-alpha Are Palmitoylated and Mediate Cytoplasmic Protein Association. J. Biol. Chem. 271: 28502-28508 [Abstract] [Full Text]
Peifley, K. A., Alberts, G. F., Hsu, D. K.W., Feng, S.-L. Y., Winkles, J. A. (1996). Heparin-Binding Epidermal Growth Factor-Like Growth Factor Regulates Fibroblast Growth Factor-2 Expression in Aortic Smooth Muscle Cells. Circ. Res. 79: 263-270 [Abstract] [Full Text]
Raab, G, Kover, K, Paria, B., Dey, S., Ezzell, R., Klagsbrun, M (1996). Mouse preimplantation blastocysts adhere to cells expressing the transmembrane form of heparin-binding EGF-like growth factor. Development 122: 637-645 [Abstract]
Wu, X.-R., Medina, J. J., Sun, T.-T. (1995). Selective Interactions of UPIa and UPIb, Two Members of the Transmembrane 4 Superfamily, with Distinct Single Transmembrane-domained Proteins in Differentiated Urothelial Cells. J. Biol. Chem. 270: 29752-29759 [Abstract] [Full Text]
Shishido, Y., Sharma, K. D., Higashiyama, S., Klagsbrun, M., Mekada, E. (1995). Heparin-like Molecules on the Cell Surface Potentiate Binding of Diphtheria Toxin to the Diphtheria Toxin Receptor/Membrane-anchored Heparin-binding Epidermal Growth Factor-like Growth Factor. J. Biol. Chem. 270: 29578-29585 [Abstract] [Full Text]
Wice, B. M., Gordon, J. I. (1995). A Tetraspan Membrane Glycoprotein Produced in the Human Intestinal Epithelium and Liver That Can Regulate Cell Density-dependent Proliferation. J. Biol. Chem. 270: 21907-21918 [Abstract] [Full Text]
Chen, X., Raab, G., Deutsch, U., Zhang, J., Ezzell, R. M., Klagsbrun, M. (1995). Induction of Heparin-binding EGF-like Growth Factor Expression during Myogenesis. J. Biol. Chem. 270: 18285-18294 [Abstract] [Full Text]
Stipp, C. S., Orlicky, D., Hemler, M. E. (2001). FPRP, a Major, Highly Stoichiometric, Highly Specific CD81- and CD9-associated Protein. J. Biol. Chem. 276: 4853-4862 [Abstract] [Full Text]
Lin, J., Hutchinson, L., Gaston, S. M., Raab, G., Freeman, M. R. (2001). BAG-1 Is a Novel Cytoplasmic Binding Partner of the Membrane Form of Heparin-binding EGF-like Growth Factor. A UNIQUE ROLE FOR proHB-EGF IN CELL SURVIVAL REGULATION. J. Biol. Chem. 276: 30127-30132 [Abstract] [Full Text]
Brown, C. L., Coffey, R. J., Dempsey, P. J. (2001). The Proamphiregulin Cytoplasmic Domain Is Required for Basolateral Sorting, but Is Not Essential for Constitutive or Stimulus-induced Processing in Polarized Madin-Darby Canine Kidney Cells. J. Biol. Chem. 276: 29538-29549 [Abstract] [Full Text]
Umata, T., Hirata, M., Takahashi, T., Ryu, F., Shida, S., Takahashi, Y., Tsuneoka, M., Miura, Y., Masuda, M., Horiguchi, Y., Mekada, E. (2001). A Dual Signaling Cascade That Regulates the Ectodomain Shedding of Heparin-binding Epidermal Growth Factor-like Growth Factor. J. Biol. Chem. 276: 30475-30482 [Abstract] [Full Text]
Stipp, C. S., Kolesnikova, T. V., Hemler, M. E. (2001). EWI-2 Is a Major CD9 and CD81 Partner and Member of a Novel Ig Protein Subfamily. J. Biol. Chem. 276: 40545-40554 [Abstract] [Full Text]
Nakamura, K., Mitamura, T., Takahashi, T., Kobayashi, T., Mekada, E. (2000). Importance of the Major Extracellular Domain of CD9 and the Epidermal Growth Factor (EGF)-like Domain of Heparin-binding EGF-like Growth Factor for Up-regulation of Binding and Activity. J. Biol. Chem. 275: 18284-18290 [Abstract] [Full Text]
Oka, M., Tagoku, K., Russell, T. L., Nakano, Y., Hamazaki, T., Meyer, E. M., Yokota, T., Terada, N. (2002). CD9 Is Associated with Leukemia Inhibitory Factor-mediated Maintenance of Embryonic Stem Cells. Mol. Biol. Cell 13: 1274-1281 [Abstract] [Full Text]