Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells

doi:10.1083/jcb.129.3.805

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Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells

C Elfgang, R Eckert, H Lichtenberg-Frate, A Butterweck, O Traub, RA Klein, DF Hulser and K Willecke
Institut fur Genetik, Abt. Molekulargenetik, Universitat Bonn, Germany.

DNAs coding for seven murine connexins (Cx) (Cx26, Cx31, Cx32, Cx37, Cx40, Cx43, and Cx45) are functionally expressed in human HeLa cells that were deficient in gap junctional communication. We compare the permeabilities of gap junctions comprised of different connexins to iontophoretically injected tracer molecules. Our results show that Lucifer yellow can pass through all connexin channels analyzed. On the other hand, propidium iodide and ethidium bromide penetrate very poorly or not at all through Cx31 and Cx32 channels, respectively, but pass through channels of other connexins. 4,6 Diamidino-2-phenylindole (DAPI) dihydrochloride shows less transfer among Cx31 or Cx43 transfectants. Neurobiotin is weakly transferred among Cx31 transfectants. Total junctional conductance in Cx31 or Cx45 transfected cells is only about half as high as in other connexin transfectants analyzed and does not correlate exactly with any of the tracer permeabilities. Permeability through different connexin channels appears to be dependent on the molecular structure of each tracer, i.e. size, charge and possibly rigidity. This supports the hypothesis that different connexin channels show different permeabilities to second messenger molecules as well as metabolites and may fulfill in this way their specific role in growth control and differentiation of cell types. In addition, we have investigated the function of heterotypic gap junctions after co-cultivation of two different connexin transfectants, one of which had been prelabeled with fluorescent dextran beads. Analysis of Lucifer yellow transfer reveals that HeLa cells expressing Cx31 (beta-type connexin) do not communicate with any other connexin transfectant tested but only with themselves. Two other beta-type connexin transfectants, HeLa-Cx26 and -Cx32, do not transmit Lucifer yellow to any of the alpha-type connexins analyzed. Among alpha- type connexins, Cx40 does not communicate with Cx43. Thus, connexins differ in their ability to form functional heterotypic gap junctions among mammalian cells.
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Lurtz, M. M., Louis, C. F. (2007). Intracellular calcium regulation of connexin43. Am. J. Physiol. Cell Physiol. 293: C1806-C1813 [Abstract] [Full Text]
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Tao, L., Harris, A. L. (2007). 2-Aminoethoxydiphenyl Borate Directly Inhibits Channels Composed of Connexin26 and/or Connexin32. Mol. Pharmacol. 71: 570-579 [Abstract] [Full Text]
Dong, L., Liu, X., Li, H., Vertel, B. M., Ebihara, L. (2006). Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels. J. Physiol. 576: 787-799 [Abstract] [Full Text]
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Larre, I., Ponce, A., Fiorentino, R., Shoshani, L., Contreras, R. G., Cereijido, M. (2006). Contacts and cooperation between cells depend on the hormone ouabain. Proc. Natl. Acad. Sci. USA 103: 10911-10916 [Abstract] [Full Text]
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Barbe, M. T., Monyer, H., Bruzzone, R. (2006). Cell-Cell Communication Beyond Connexins: The Pannexin Channels. Physiology 21: 103-114 [Abstract] [Full Text]
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Haass, N. K., Wladykowski, E., Kief, S., Moll, I., Brandner, J. M. (2006). Differential Induction of Connexins 26 and 30 in Skin Tumors and Their Adjacent Epidermis. J. Histochem. Cytochem. 54: 171-182 [Abstract] [Full Text]
Betsuyaku, T., Nnebe, N. S., Sundset, R., Patibandla, S., Krueger, C. M., Yamada, K. A. (2006). Overexpression of cardiac connexin45 increases susceptibility to ventricular tachyarrhythmias in vivo. Am. J. Physiol. Heart Circ. Physiol. 290: H163-H171 [Abstract] [Full Text]
Ek-Vitorin, J. F., Burt, J. M. (2005). Quantification of gap junction selectivity. Am. J. Physiol. Cell Physiol. 289: C1535-C1546 [Abstract] [Full Text]
Gittens, J. E. I., Kidder, G. M. (2005). Differential contributions of connexin37 and connexin43 to oogenesis revealed in chimeric reaggregated mouse ovaries. J. Cell Sci. 118: 5071-5078 [Abstract] [Full Text]
Valiunas, V, Polosina, Y. Y, Miller, H, Potapova, I. A, Valiuniene, L, Doronin, S, Mathias, R. T, Robinson, R. B, Rosen, M. R, Cohen, I. S, Brink, P. R (2005). Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions. J. Physiol. 568: 459-468 [Abstract] [Full Text]
Lahlou, H., Fanjul, M., Pradayrol, L., Susini, C., Pyronnet, S. (2005). Restoration of Functional Gap Junctions through Internal Ribosome Entry Site-Dependent Synthesis of Endogenous Connexins in Density-Inhibited Cancer Cells. Mol. Cell. Biol. 25: 4034-4045 [Abstract] [Full Text]
Thimm, J., Mechler, A., Lin, H., Rhee, S., Lal, R. (2005). Calcium-dependent Open/Closed Conformations and Interfacial Energy Maps of Reconstituted Hemichannels. J. Biol. Chem. 280: 10646-10654 [Abstract] [Full Text]
Houghton, F. D (2005). Role of gap junctions during early embryo development. Reproduction 129: 129-135 [Abstract] [Full Text]
Araya, R., Eckardt, D., Maxeiner, S., Kruger, O., Theis, M., Willecke, K., Saez, J. C. (2005). Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43. J. Cell Sci. 118: 27-37 [Abstract] [Full Text]
von Maltzahn, J., Euwens, C., Willecke, K., Sohl, G. (2004). The novel mouse connexin39 gene is expressed in developing striated muscle fibers. J. Cell Sci. 117: 5381-5392 [Abstract] [Full Text]
Gellhaus, A., Dong, X., Propson, S., Maass, K., Klein-Hitpass, L., Kibschull, M., Traub, O., Willecke, K., Perbal, B., Lye, S. J., Winterhager, E. (2004). Connexin43 Interacts with NOV: A POSSIBLE MECHANISM FOR NEGATIVE REGULATION OF CELL GROWTH IN CHORIOCARCINOMA CELLS. J. Biol. Chem. 279: 36931-36942 [Abstract] [Full Text]
Essenfelder, G. M., Bruzzone, R., Lamartine, J., Charollais, A., Blanchet-Bardon, C., Barbe, M. T., Meda, P., Waksman, G. (2004). Connexin30 mutations responsible for hidrotic ectodermal dysplasia cause abnormal hemichannel activity. Hum Mol Genet 13: 1703-1714 [Abstract] [Full Text]
Veitch, G. I., Gittens, J. E. I., Shao, Q., Laird, D. W., Kidder, G. M. (2004). Selective assembly of connexin37 into heterocellular gap junctions at the oocyte/granulosa cell interface. J. Cell Sci. 117: 2699-2707 [Abstract] [Full Text]
Locke, D., Koreen, I. V., Liu, J. Y., Harris, A. L. (2004). Reversible Pore Block of Connexin Channels by Cyclodextrins. J. Biol. Chem. 279: 22883-22892 [Abstract] [Full Text]
Gemel, J., Valiunas, V., Brink, P. R., Beyer, E. C. (2004). Connexin43 and connexin26 form gap junctions, but not heteromeric channels in co-expressing cells. J. Cell Sci. 117: 2469-2480 [Abstract] [Full Text]
Saitongdee, P., Becker, D. L., Milner, P., Knight, G. E., Burnstock, G. (2004). Levels of Gap Junction Proteins in Coronary Arterioles and Aorta of Hamsters Exposed to the Cold and During Hibernation and Arousal. J. Histochem. Cytochem. 52: 603-616 [Abstract] [Full Text]
Sohl, G., Willecke, K. (2004). Gap junctions and the connexin protein family. Cardiovasc Res 62: 228-232 [Abstract] [Full Text]
Vink, M. J, Suadicani, S. O, Vieira, D. M, Urban-Maldonado, M., Gao, Y., Fishman, G. I, Spray, D. C (2004). Alterations of intercellular communication in neonatal cardiac myocytes from connexin43 null mice. Cardiovasc Res 62: 397-406 [Abstract] [Full Text]
Thomas, T., Telford, D., Laird, D. W. (2004). Functional Domain Mapping and Selective Trans-dominant Effects Exhibited by Cx26 Disease-causing Mutations. J. Biol. Chem. 279: 19157-19168 [Abstract] [Full Text]
Zhang, Z.-Q., Hu, Y., Wang, B.-J., Lin, Z.-X., Naus, C. C.G., Nicholson, B. J. (2004). Effective asymmetry in gap junctional intercellular communication between populations of human normal lung fibroblasts and lung carcinoma cells. Carcinogenesis 25: 473-482 [Abstract] [Full Text]
Dagli, M. L. Z., Yamasaki, H., Krutovskikh, V., Omori, Y. (2004). Delayed liver regeneration and increased susceptibility to chemical hepatocarcinogenesis in transgenic mice expressing a dominant-negative mutant of connexin32 only in the liver. Carcinogenesis 25: 483-492 [Abstract] [Full Text]
Valiunas, V., Doronin, S., Valiuniene, L., Potapova, I., Zuckerman, J., Walcott, B., Robinson, R. B., Rosen, M. R., Brink, P. R., Cohen, I. S. (2004). Human mesenchymal stem cells make cardiac connexins and form functional gap junctions. J. Physiol. 555: 617-626 [Abstract] [Full Text]
Lin, G. C., Rurangirwa, J. K., Koval, M., Steinberg, T. H. (2004). Gap junctional communication modulates agonist-induced calcium oscillations in transfected HeLa cells. J. Cell Sci. 117: 881-887 [Abstract] [Full Text]
Hoffmann, A., Gloe, T., Pohl, U., Zahler, S. (2003). Nitric oxide enhances de novo formation of endothelial gap junctions. Cardiovasc Res 60: 421-430 [Abstract] [Full Text]
SAEZ, J. C., BERTHOUD, V. M., BRANES, M. C., MARTINEZ, A. D., BEYER, E. C. (2003). Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions. Physiol. Rev. 83: 1359-1400 [Abstract] [Full Text]
Le Gurun, S., Martin, D., Formenton, A., Maechler, P., Caille, D., Waeber, G., Meda, P., Haefliger, J.-A. (2003). Connexin-36 Contributes to Control Function of Insulin-producing Cells. J. Biol. Chem. 278: 37690-37697 [Abstract] [Full Text]
Kretz, M., Euwens, C., Hombach, S., Eckardt, D., Teubner, B., Traub, O., Willecke, K., Ott, T. (2003). Altered connexin expression and wound healing in the epidermis of connexin-deficient mice. J. Cell Sci. 116: 3443-3452 [Abstract] [Full Text]
Yeh, H.-I, Lu, C.-S., Wu, Y.-J., Chen, C.-C., Hong, R.-C., Ko, Y.-S., Shiao, M.-S., Severs, N. J., Tsai, C.-H. (2003). Reduced Expression of Endothelial Connexin37 and Connexin40 in Hyperlipidemic Mice: Recovery of Connexin37 After 7-Day Simvastatin Treatment. Arterioscler. Thromb. Vasc. Bio. 23: 1391-1397 [Abstract] [Full Text]
Martinez-Wittinghan, F. J., Sellitto, C., Li, L., Gong, X., Brink, P. R., Mathias, R. T., White, T. W. (2003). Dominant cataracts result from incongruous mixing of wild-type lens connexins. JCB 161: 969-978 [Abstract] [Full Text]
Marziano, N. K., Casalotti, S. O., Portelli, A. E., Becker, D. L., Forge, A. (2003). Mutations in the gene for connexin 26 (GJB2) that cause hearing loss have a dominant negative effect on connexin 30. Hum Mol Genet 12: 805-812 [Abstract] [Full Text]
Yeh, H.-I., Lai, Y.-J., Lee, Y.-N., Chen, Y.-J., Chen, Y.-C., Chen, C.-C., Chen, S.-A., Lin, C.-I., Tsai, C.-H. (2003). Differential Expression of Connexin43 Gap Junctions in Cardiomyocytes Isolated from Canine Thoracic Veins. J. Histochem. Cytochem. 51: 259-266 [Abstract] [Full Text]
Lin, J. H.-C., Yang, J., Liu, S., Takano, T., Wang, X., Gao, Q., Willecke, K., Nedergaard, M. (2003). Connexin Mediates Gap Junction-Independent Resistance to Cellular Injury. J. Neurosci. 23: 430-441 [Abstract] [Full Text]
Zahler, S., Hoffmann, A., Gloe, T., Pohl, U. (2003). Gap-junctional coupling between neutrophils and endothelial cells: a novel modulator of transendothelial migration. J. Leukoc. Biol. 73: 118-126 [Abstract] [Full Text]
Koval, M. (2002). Sharing signals: connecting lung epithelial cells with gap junction channels. Am. J. Physiol. Lung Cell. Mol. Physiol. 283: L875-L893 [Abstract] [Full Text]
Skerrett, I.M., Aronowitz, J., Shin, J.H., Cymes, G., Kasperek, E., Cao, F.L., Nicholson, B.J. (2002). Identification of amino acid residues lining the pore of a gap junction channel. JCB 159: 349-360 [Abstract] [Full Text]
Nielsen, P. A., Beahm, D. L., Giepmans, B. N. G., Baruch, A., Hall, J. E., Kumar, N. M. (2002). Molecular Cloning, Functional Expression, and Tissue Distribution of a Novel Human Gap Junction-forming Protein, Connexin-31.9. INTERACTION WITH ZONA OCCLUDENS PROTEIN-1. J. Biol. Chem. 277: 38272-38283 [Abstract] [Full Text]
Goldberg, G. S., Moreno, A. P., Lampe, P. D. (2002). Gap Junctions between Cells Expressing Connexin 43 or 32 Show Inverse Permselectivity to Adenosine and ATP. J. Biol. Chem. 277: 36725-36730 [Abstract] [Full Text]
Risley, M. S., Tan, I. P., Farrell, J. (2002). Gap Junctions with Varied Permeability Properties Establish Cell-Type Specific Communication Pathways in the Rat Seminiferous Epithelium. Biol. Reprod. 67: 945-952 [Abstract] [Full Text]
Kwak, B. R, Shah, D. C, Mach, F. (2002). A starting point for structure function relationships in the canine pulmonary veins. Cardiovasc Res 55: 703-705 [Full Text]
Lin, J. H.-C., Takano, T., Cotrina, M. L., Arcuino, G., Kang, J., Liu, S., Gao, Q., Jiang, L., Li, F., Lichtenberg-Frate, H., Haubrich, S., Willecke, K., Goldman, S. A., Nedergaard, M. (2002). Connexin 43 Enhances the Adhesivity and Mediates the Invasion of Malignant Glioma Cells. J. Neurosci. 22: 4302-4311 [Abstract] [Full Text]
Martinez, A. D., Hayrapetyan, V., Moreno, A. P., Beyer, E. C. (2002). Connexin43 and Connexin45 Form Heteromeric Gap Junction Channels in Which Individual Components Determine Permeability and Regulation. Circ. Res. 90: 1100-1107 [Abstract] [Full Text]
Bukauskas, F. F., Bukauskiene, A., Verselis, V. K., Bennett, M. V. L. (2002). Coupling asymmetry of heterotypic connexin 45/ connexin 43-EGFP gap junctions: Properties of fast and slow gating mechanisms. Proc. Natl. Acad. Sci. USA 99: 7113-7118 [Abstract] [Full Text]
Leite, M. F., Hirata, K., Pusl, T., Burgstahler, A. D., Okazaki, K., Ortega, J. M., Goes, A. M., Prado, M. A. M., Spray, D. C., Nathanson, M. H. (2002). Molecular Basis for Pacemaker Cells in Epithelia. J. Biol. Chem. 277: 16313-16323 [Abstract] [Full Text]
Houghton, F.D., Barr, K.J., Walter, G., Gabriel, H.-D., Grummer, R., Traub, O., Leese, H.J., Winterhager, E., Kidder, G.M. (2002). Functional Significance of Gap Junctional Coupling in Preimplantation Development. Biol. Reprod. 66: 1403-1412 [Abstract] [Full Text]
Plotkin, L. I., Manolagas, S. C., Bellido, T. (2002). Transduction of Cell Survival Signals by Connexin-43 Hemichannels. J. Biol. Chem. 277: 8648-8657 [Abstract] [Full Text]
Johnson, C. M., Kanter, E. M., Green, K. G., Laing, J. G., Betsuyaku, T., Beyer, E. C., Steinberg, T. H., Saffitz, J. E., Yamada, K. A. (2002). Redistribution of connexin45 in gap junctions of connexin43-deficient hearts. Cardiovasc Res 53: 921-935 [Abstract] [Full Text]
Muramatsu, A., Iwai, M., Morikawa, T., Tanaka, S., Mori, T., Harada, Y., Okanoue, T. (2002). Influence of transfection with connexin 26 gene on malignant potential of human hepatoma cells. Carcinogenesis 23: 351-358 [Abstract] [Full Text]
Kwak, B. R., Mulhaupt, F., Veillard, N., Gros, D. B., Mach, F. (2002). Altered Pattern of Vascular Connexin Expression in Atherosclerotic Plaques. Arterioscler. Thromb. Vasc. Bio. 22: 225-230 [Abstract] [Full Text]
Contreras, J. E., Sanchez, H. A., Eugenin, E. A., Speidel, D., Theis, M., Willecke, K., Bukauskas, F. F., Bennett, M. V. L., Saez, J. C. (2001). Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture. Proc. Natl. Acad. Sci. USA 10.1073/pnas.012589799v1 [Abstract] [Full Text]
Bondurand, N., Girard, M., Pingault, V., Lemort, N., Dubourg, O., Goossens, M. (2001). Human Connexin 32, a gap junction protein altered in the X-linked form of Charcot-Marie-Tooth disease, is directly regulated by the transcription factor SOX10. Hum Mol Genet 10: 2783-2795 [Abstract] [Full Text]
Cottrell, G. T., Burt, J. M. (2001). Heterotypic gap junction channel formation between heteromeric and homomeric Cx40 and Cx43 connexons. Am. J. Physiol. Cell Physiol. 281: C1559-C1567 [Abstract] [Full Text]
Chen-Izu, Y., Moreno, A. P., Spangler, R. A. (2001). Opposing gates model for voltage gating of gap junction channels. Am. J. Physiol. Cell Physiol. 281: C1604-C1613 [Abstract] [Full Text]
Li, X., Simard, J. M. (2001). Connexin45 gap junction channels in rat cerebral vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 281: H1890-H1898 [Abstract] [Full Text]
Isakson, B. E., Lubman, R. L., Seedorf, G. J., Boitano, S. (2001). Modulation of pulmonary alveolar type II cell phenotype and communication by extracellular matrix and KGF. Am. J. Physiol. Cell Physiol. 281: C1291-C1299 [Abstract] [Full Text]
Valiunas, V., Gemel, J., Brink, P. R., Beyer, E. C. (2001). Gap junction channels formed by coexpressed connexin40 and connexin43. Am. J. Physiol. Heart Circ. Physiol. 281: H1675-H1689 [Abstract] [Full Text]
Churchill, G. C., Lurtz, M. M., Louis, C. F. (2001). Ca2+ regulation of gap junctional coupling in lens epithelial cells. Am. J. Physiol. Cell Physiol. 281: C972-C981 [Abstract] [Full Text]
van Veen, T. A.B., van Rijen, H. V.M., Opthof, T. (2001). Cardiac gap junction channels: modulation of expression and channel properties. Cardiovasc Res 51: 217-229 [Abstract] [Full Text]
Abraham, V., Chou, M. L., George, P., Pooler, P., Zaman, A., Savani, R. C., Koval, M. (2001). Heterocellular gap junctional communication between alveolar epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 280: L1085-L1093 [Abstract] [Full Text]
Chaytor, A. T., Martin, P. E. M., Edwards, D. H., Griffith, T. M. (2001). Gap junctional communication underpins EDHF-type relaxations evoked by ACh in the rat hepatic artery. Am. J. Physiol. Heart Circ. Physiol. 280: H2441-H2450 [Abstract] [Full Text]
Kwak, B. R., Pepper, M. S., Gros, D. B., Meda, P. (2001). Inhibition of Endothelial Wound Repair by Dominant Negative Connexin Inhibitors. Mol. Biol. Cell 12: 831-845 [Abstract] [Full Text]
Rash, J. E., Yasumura, T., Dudek, F. E., Nagy, J. I. (2001). Cell-Specific Expression of Connexins and Evidence of Restricted Gap Junctional Coupling between Glial Cells and between Neurons. J. Neurosci. 21: 1983-2000 [Abstract] [Full Text]
Princen, F., Robe, P., Gros, D., Jarry-Guichard, T., Gielen, J., Merville, M.-P., Bours, V. (2001). Rat gap junction connexin-30 inhibits proliferation of glioma cell lines. Carcinogenesis 22: 507-513 [Abstract] [Full Text]
Burt, J. M., Fletcher, A. M., Steele, T. D., Wu, Y., Cottrell, G. T., Kurjiaka, D. T. (2001). Alteration of Cx43:Cx40 expression ratio in A7r5 cells. Am. J. Physiol. Cell Physiol. 280: C500-C508 [Abstract] [Full Text]
Martin, P. E. M., Blundell, G., Ahmad, S., Errington, R. J., Evans, W. H. (2001). Multiple pathways in the trafficking and assembly of connexin 26, 32 and 43 into gap junction intercellular communication channels. J. Cell Sci. 114: 3845-3855 [Abstract] [Full Text]
Jordan, K, Chodock, R, Hand, A., Laird, D. (2001). The origin of annular junctions: a mechanism of gap junction internalization. J. Cell Sci. 114: 763-773 [Abstract]