Chemoattractant and GTP gamma S-mediated stimulation of adenylyl cyclase in Dictyostelium requires translocation of CRAC to membranes

doi:10.1083/jcb.129.6.1659

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 812K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lilly, P. J.
	Articles by Devreotes, P. N.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lilly, P. J.
	Articles by Devreotes, P. N.


Social Bookmarking

	What's this?

ARTICLES

Chemoattractant and GTP gamma S-mediated stimulation of adenylyl cyclase in Dictyostelium requires translocation of CRAC to membranes

PJ Lilly and PN Devreotes
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

We have previously reported that activation of adenylyl cyclase by chemoattractant receptors in Dictyostelium requires, in addition to a heterotrimeric G-protein, a cytosolic protein, designated CRAC (Lilly, P., and P. N. Devreotes. 1994. J. Biol. Chem. 269:14123-14129; Insall, R. H., A. Kuspa, P. J. Lilly, G. Schaulsky, L. R. Levin, W. F. Loomis, and P. N. Devreotes. 1994. J. Cell Biol. 126:1537-1545). In this report, we show that in intact cells, chemoattractants promote translocation of CRAC from the cytosolic to the membrane fraction. However, CRAC is not required at the time of receptor stimulation; it can be added to lysates of activated cells. Treatment of membranes with guanine nucleotides creates binding sites for CRAC. These binding sites can be generated in mutants lacking each of the components of the pathway except the beta-subunit, suggesting that free or "activated" beta gamma-subunits may be a part of the binding site. This hypothesis is consistent with previous observations that CRAC contains a pleckstrin homology domain and that the beta gamma-subunits likely mediate activation of adenylyl cyclase in this system. Thus, CRAC may serve as an adapter, linking the G-protein beta gamma-subunits to activation of the enzyme. GTP gamma S cannot generate CRAC-binding sites when the adenylyl cyclase pathway has been adapted by prior chemoattractant stimulation, suggesting that this is a point of downstream adaptation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Kawabe, Y., Morio, T., James, J. L., Prescott, A. R., Tanaka, Y., Schaap, P. (2009). Activated cAMP receptors switch encystation into sporulation. Proc. Natl. Acad. Sci. USA 106: 7089-7094 [Abstract] [Full Text]
Tang, Y., Gomer, R. H. (2008). A Protein with Similarity to PTEN Regulates Aggregation Territory Size by Decreasing Cyclic AMP Pulse Size during Dictyostelium discoideum Development. Eukaryot Cell 7: 1758-1770 [Abstract] [Full Text]
Ghosh, P., Garcia-Marcos, M., Bornheimer, S. J., Farquhar, M. G. (2008). Activation of G{alpha}i3 triggers cell migration via regulation of GIV. JCB 182: 381-393 [Abstract] [Full Text]
Mahadeo, D. C., Janka-Junttila, M., Smoot, R. L., Roselova, P., Parent, C. A. (2007). A Chemoattractant-mediated Gi-coupled Pathway Activates Adenylyl Cyclase in Human Neutrophils. Mol. Biol. Cell 18: 512-522 [Abstract] [Full Text]
Samadani, A., Mettetal, J., van Oudenaarden, A. (2006). Cellular asymmetry and individuality in directional sensing. Proc. Natl. Acad. Sci. USA 103: 11549-11554 [Abstract] [Full Text]
Matsuoka, S., Iijima, M., Watanabe, T. M., Kuwayama, H., Yanagida, T., Devreotes, P. N., Ueda, M. (2006). Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein. J. Cell Sci. 119: 1071-1079 [Abstract] [Full Text]
Comer, F. I., Parent, C. A. (2006). Phosphoinositide 3-Kinase Activity Controls the Chemoattractant-mediated Activation and Adaptation of Adenylyl Cyclase. Mol. Biol. Cell 17: 357-366 [Abstract] [Full Text]
Lee, S., Comer, F. I., Sasaki, A., McLeod, I. X., Duong, Y., Okumura, K., Yates, J. R. III, Parent, C. A., Firtel, R. A. (2005). TOR Complex 2 Integrates Cell Movement during Chemotaxis and Signal Relay in Dictyostelium. Mol. Biol. Cell 16: 4572-4583 [Abstract] [Full Text]
Gao, T., Knecht, D., Tang, L., Hatton, R. D., Gomer, R. H. (2004). A Cell Number Counting Factor Regulates Akt/Protein Kinase B To Regulate Dictyostelium discoideum Group Size. Eukaryot Cell 3: 1176-1184 [Abstract] [Full Text]
Merlot, S., Firtel, R. A. (2003). Leading the way: directional sensing through phosphatidylinositol 3-kinase and other signaling pathways. J. Cell Sci. 116: 3471-3478 [Abstract] [Full Text]
Devreotes, P., Janetopoulos, C. (2003). Eukaryotic Chemotaxis: Distinctions between Directional Sensing and Polarization. J. Biol. Chem. 278: 20445-20448 [Abstract] [Full Text]
Huang, Y. E., Iijima, M., Parent, C. A., Funamoto, S., Firtel, R. A., Devreotes, P. (2003). Receptor-mediated Regulation of PI3Ks Confines PI(3,4,5)P3 to the Leading Edge of Chemotaxing Cells. Mol. Biol. Cell 14: 1913-1922 [Abstract] [Full Text]
Funamoto, S., Milan, K., Meili, R., Firtel, R. A. (2001). Role of Phosphatidylinositol 3' Kinase and a Downstream Pleckstrin Homology Domain-Containing Protein in Controlling Chemotaxis inDictyostelium. JCB 153: 795-810 [Abstract] [Full Text]
Janetopoulos, C., Jin, T., Devreotes, P. (2001). Receptor-Mediated Activation of Heterotrimeric G-Proteins in Living Cells. Science 291: 2408-2411 [Abstract] [Full Text]
Noegel, A., Schleicher, M (2000). The actin cytoskeleton of Dictyostelium: a story told by mutants. J. Cell Sci. 113: 759-766 [Abstract]
Lee, S., Parent, C. A., Insall, R., Firtel, R. A. (1999). A Novel Ras-interacting Protein Required for Chemotaxis and Cyclic Adenosine Monophosphate Signal Relay in Dictyostelium. Mol. Biol. Cell 10: 2829-2845 [Abstract] [Full Text]
Parent, C. A., Devreotes, P. N. (1999). A Cell's Sense of Direction. Science 284: 765-770 [Abstract] [Full Text]
Pryciak, P. M., Huntress, F. A. (1998). Membrane recruitment of the kinase cascade scaffold protein Ste5 by the Gbeta gamma complex underlies activation of the yeast pheromone response pathway. Genes Dev. 12: 2684-2697 [Abstract] [Full Text]
Chen, M.-Y., Long, Y., Devreotes, P. N. (1997). A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12�transmembrane domain adenylyl cyclase in�Dictyostelium. Genes Dev. 11: 3218-3231 [Abstract] [Full Text]
Kim, J.-Y., Soede, R. D.�M., Schaap, P., Valkema, R., Borleis, J. A., Van Haastert, P. J.�M., Devreotes, P. N., Hereld, D. (1997). Phosphorylation of Chemoattractant Receptors Is Not Essential for Chemotaxis or Termination of G-protein-mediated Responses. J. Biol. Chem. 272: 27313-27318 [Abstract] [Full Text]
Parent, C. A., Devreotes, P. N. (1996). Constitutively Active Adenylyl Cyclase Mutant Requires Neither G Proteins nor Cytosolic Regulators. J. Biol. Chem. 271: 18333-18336 [Abstract] [Full Text]
Parent, C. A., Devreotes, P. N. (1995). Isolation of Inactive and G Protein-resistant Adenylyl Cyclase Mutants Using Random Mutagenesis. J. Biol. Chem. 270: 22693-22696 [Abstract] [Full Text]
van Es, S., Weening, K. E., Devreotes, P. N. (2001). The Protein Kinase YakA Regulates G-protein-linked Signaling Responses during Growth and Development of Dictyostelium. J. Biol. Chem. 276: 30761-30765 [Abstract] [Full Text]
Parent, C. A., Borleis, J., Devreotes, P. N. (2002). Regulation of Adenylyl Cyclases by a Region Outside the Minimally Functional Cytoplasmic Domains. J. Biol. Chem. 277: 1354-1360 [Abstract] [Full Text]