The G protein beta subunit is essential for multiple responses to chemoattractants in Dictyostelium

doi:10.1083/jcb.129.6.1667

This Article

	Full Text (PDF, 1225K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wu, L.
	Articles by Devreotes, P. N.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wu, L.
	Articles by Devreotes, P. N.


Social Bookmarking

	What's this?

ARTICLES

The G protein beta subunit is essential for multiple responses to chemoattractants in Dictyostelium

L Wu, R Valkema, PJ Van Haastert and PN Devreotes
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA.

Increasing evidence suggests that the beta gamma-subunit dimers of heterotrimeric G proteins play a pivotal role in transducing extracellular signals. The recent construction of G beta null mutants (g beta-) in Dictyostelium provides a unique opportunity to study the role of beta gamma dimers in signaling processes mediated by chemoattractant receptors. We have shown previously that g beta- cells fail to aggregate; in this study, we report the detailed characterization of these cells. The g beta- cells display normal motility but do not move towards chemattractants. The typical GTP- regulated high affinity chemoattractant-binding sites are lost in g beta- cells and membranes. The g beta- cells do not display chemoattractant-stimulated adenylyl cyclase or guanylyl cyclase activity. These results show that in vivo G beta links chemoattractant receptors to effectors and is therefore essential in many chemoattractant-mediated processes. In addition, we find that G beta is required for GTP gamma S stimulation of adenylyl cyclase activity, suggesting that the beta gamma-dimer activates the enzyme directly. Interestingly, the g beta- cells grow at the same rate as wild-type cells in axenic medium but grow more slowly on bacterial lawns and, therefore, may be defective in phagocytosis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Wang, F. (2009). The Signaling Mechanisms Underlying Cell Polarity and Chemotaxis. Cold Spring Harb. Perspect. Biol. 1: a002980-a002980 [Abstract] [Full Text]
Bolourani, P., Spiegelman, G. B., Weeks, G. (2008). Rap1 Activation in Response to cAMP Occurs Downstream of Ras Activation during Dictyostelium Aggregation. J. Biol. Chem. 283: 10232-10240 [Abstract] [Full Text]
Hsueh, Y.-P., Xue, C., Heitman, J. (2007). G protein signaling governing cell fate decisions involves opposing G{alpha} subunits in Cryptococcus neoformans. Mol. Biol. Cell 18: 3237-3249 [Abstract] [Full Text]
Sasaki, A. T., Janetopoulos, C., Lee, S., Charest, P. G., Takeda, K., Sundheimer, L. W., Meili, R., Devreotes, P. N., Firtel, R. A. (2007). G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility. JCB 178: 185-191 [Abstract] [Full Text]
Mahadeo, D. C., Janka-Junttila, M., Smoot, R. L., Roselova, P., Parent, C. A. (2007). A Chemoattractant-mediated Gi-coupled Pathway Activates Adenylyl Cyclase in Human Neutrophils. Mol. Biol. Cell 18: 512-522 [Abstract] [Full Text]
Gotthardt, D., Blancheteau, V., Bosserhoff, A., Ruppert, T., Delorenzi, M., Soldati, T. (2006). Proteomics Fingerprinting of Phagosome Maturation and Evidence for the Role of a G{alpha} during Uptake. Mol. Cell. Proteomics 5: 2228-2243 [Abstract] [Full Text]
Levine, H., Kessler, D. A., Rappel, W.-J. (2006). Directional sensing in eukaryotic chemotaxis: A balanced inactivation model. Proc. Natl. Acad. Sci. USA 103: 9761-9766 [Abstract] [Full Text]
Knol, J. C., Engel, R., Blaauw, M., Visser, A. J. W. G., van Haastert, P. J. M. (2005). The Phosducin-Like Protein PhLP1 Is Essential for G{beta}{gamma} Dimer Formation in Dictyostelium discoideum. Mol. Cell. Biol. 25: 8393-8400 [Abstract] [Full Text]
Fache, S., Dalous, J., Engelund, M., Hansen, C., Chamaraux, F., Fourcade, B., Satre, M., Devreotes, P., Bruckert, F. (2005). Calcium mobilization stimulates Dictyostelium discoideum shear-flow-induced cell motility. J. Cell Sci. 118: 3445-3458 [Abstract] [Full Text]
Zhang, M., Goswami, M., Hereld, D. (2005). Constitutively Active G Protein-coupled Receptor Mutants Block Dictyostelium Development. Mol. Biol. Cell 16: 562-572 [Abstract] [Full Text]
Chen, S., Dell, E. J., Lin, F., Sai, J., Hamm, H. E. (2004). RACK1 Regulates Specific Functions of G{beta}{gamma}. J. Biol. Chem. 279: 17861-17868 [Abstract] [Full Text]
Brzostowski, J. A., Parent, C. A., Kimmel, A. R. (2004). A G{alpha}-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement. Genes Dev. 18: 805-815 [Abstract] [Full Text]
Zhao, M., Jin, T., McCaig, C. D., Forrester, J. V., Devreotes, P. N. (2002). Genetic analysis of the role of G protein-coupled receptor signaling in electrotaxis. JCB 157: 921-928 [Abstract] [Full Text]
Yang, Q., Poole, S. I., Borkovich, K. A. (2002). A G-Protein {beta} Subunit Required for Sexual and Vegetative Development and Maintenance of Normal G{alpha} Protein Levels in Neurospora crassa. Eukaryot Cell 1: 378-390 [Abstract] [Full Text]
(2002). Genetically Modified Animals in Endocrinology. Endocr. Rev. 23: 276-278 [Full Text]
Roelofs, J., Van Haastert, P. J. M. (2002). Characterization of Two Unusual Guanylyl Cyclases from Dictyostelium. J. Biol. Chem. 277: 9167-9174 [Abstract] [Full Text]
Ueda, M., Sako, Y., Tanaka, T., Devreotes, P., Yanagida, T. (2001). Single-Molecule Analysis of Chemotactic Signaling in Dictyostelium Cells. Science 294: 864-867 [Abstract] [Full Text]
Zhang, N., Long, Y., Devreotes, P. N. (2001). Ggamma in Dictyostelium: Its Role in Localization of Gbeta gamma to the Membrane Is Required for Chemotaxis in Shallow Gradients. Mol. Biol. Cell 12: 3204-3213 [Abstract] [Full Text]
van Es, S., Wessels, D., Soll, D. R., Borleis, J., Devreotes, P. N. (2001). Tortoise, a Novel Mitochondrial Protein, Is Required for Directional Responses of Dictyostelium in Chemotactic Gradients. JCB 152: 621-632 [Abstract] [Full Text]
KOVALA, A. T., HARVEY, K. A., McGLYNN, P., BOGUSLAWSKI, G., GARCIA, J. G. N., ENGLISH, D. (2000). High-efficiency transient transfection of endothelial cells for functional analysis. FASEB J. 14: 2486-2494 [Abstract] [Full Text]
Solomon, J. M., Rupper, A., Cardelli, J. A., Isberg, R. R. (2000). Intracellular Growth of Legionella pneumophila in Dictyostelium discoideum, a System for Genetic Analysis of Host-Pathogen Interactions. Infect. Immun. 68: 2939-2947 [Abstract] [Full Text]
Chien, S., Chung, C. Y., Sukumaran, S., Osborne, N., Lee, S., Ellsworth, C., McNally, J. G., Firtel, R. A. (2000). The Dictyostelium LIM Domain-containing Protein LIM2 Is Essential for Proper Chemotaxis and Morphogenesis. Mol. Biol. Cell 11: 1275-1291 [Abstract] [Full Text]
Jin, T., Zhang, N., Long, Y., Parent, C. A., Devreotes, P. N. (2000). Localization of the G Protein Complex in Living Cells During Chemotaxis. Science 287: 1034-1036 [Abstract] [Full Text]
Wang, P., Perfect, J. R., Heitman, J. (2000). The G-Protein beta Subunit GPB1 Is Required for Mating and Haploid Fruiting in Cryptococcus neoformans. Mol. Cell. Biol. 20: 352-362 [Abstract] [Full Text]
Lee, S., Parent, C. A., Insall, R., Firtel, R. A. (1999). A Novel Ras-interacting Protein Required for Chemotaxis and Cyclic Adenosine Monophosphate Signal Relay in Dictyostelium. Mol. Biol. Cell 10: 2829-2845 [Abstract] [Full Text]
Nakamura, T., Takeuchi, K., Muraoka, S., Takezoe, H., Takahashi, N., Mori, N. (1999). A Neurally Enriched Coronin-like Protein, ClipinC, Is a Novel Candidate for an Actin Cytoskeleton-Cortical Membrane-linking Protein. J. Biol. Chem. 274: 13322-13327 [Abstract] [Full Text]
Parent, C. A., Devreotes, P. N. (1999). A Cell's Sense of Direction. Science 284: 765-770 [Abstract] [Full Text]
Seastone, D. J., Zhang, L., Buczynski, G., Rebstein, P., Weeks, G., Spiegelman, G., Cardelli, J. (1999). The Small Mr Ras-like GTPase Rap1 and the Phospholipase C Pathway Act to Regulate Phagocytosis in Dictyostelium discoideum. Mol. Biol. Cell 10: 393-406 [Abstract] [Full Text]
Xiao, Z., Yao, Y., Long, Y., Devreotes, P. (1999). Desensitization of G-protein-coupled Receptors. AGONIST-INDUCED PHOSPHORYLATION OF THE CHEMOATTRACTANT RECEPTOR cAR1 LOWERS ITS INTRINSIC AFFINITY FOR cAMP. J. Biol. Chem. 274: 1440-1448 [Abstract] [Full Text]
Jin, T., Amzel, M., Devreotes, P. N., Wu, L. (1998). Selection of Gbeta Subunits with Point Mutations That Fail to Activate Specific Signaling Pathways In Vivo: Dissecting Cellular Responses Mediated by a Heterotrimeric G Protein in Dictyostelium discoideum. Mol. Biol. Cell 9: 2949-2961 [Abstract] [Full Text]
Peracino, B., Borleis, J., Jin, T., Westphal, M., Schwartz, J.-M., Wu, L., Bracco, E., Gerisch, G., Devreotes, P., Bozzaro, S. (1998). G Protein {beta} Subunit-null Mutants Are Impaired in Phagocytosis and Chemotaxis Due to Inappropriate Regulation of the Actin Cytoskeleton. JCB 141: 1529-1537 [Abstract] [Full Text]
Chen, M.-Y., Long, Y., Devreotes, P. N. (1997). A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12�transmembrane domain adenylyl cyclase in�Dictyostelium. Genes Dev. 11: 3218-3231 [Abstract] [Full Text]
Maeda, M., Firtel, R. A. (1997). Activation of the Mitogen-activated Protein Kinase ERK2 by the Chemoattractant Folic Acid in Dictyostelium. J. Biol. Chem. 272: 23690-23695 [Abstract] [Full Text]
Zigmond, S. H., Joyce, M., Borleis, J., Bokoch, G. M., Devreotes, P. N. (1997). Regulation of Actin Polymerization in Cell-free Systems by GTP{gamma}S and Cdc42. JCB 138: 363-374 [Abstract] [Full Text]
Buczynski, G., Grove, B., Nomura, A., Kleve, M., Bush, J., Firtel, R. A., Cardelli, J. (1997). Inactivation of Two Dictyostelium discoideum Genes, DdPIK1 and DdPIK2, Encoding Proteins Related to Mammalian Phosphatidylinositide 3-kinases, Results in Defects in Endocytosis, Lysosome to Postlysosome Transport, and Actin Cytoskeleton Organization. JCB 136: 1271-1286 [Abstract] [Full Text]
Kim, J.-Y., Caterina, M. J., Milne, J. L.S., Lin, K. C., Borleis, J. A., Devreotes, P. N. (1997). Random Mutagenesis of the cAMP Chemoattractant Receptor, cAR1, of Dictyostelium. MUTANT CLASSES THAT CAUSE DISCRETE SHIFTS IN AGONIST AFFINITY AND LOCK THE RECEPTOR IN A NOVEL ACTIVATIONAL INTERMEDIATE. J. Biol. Chem. 272: 2060-2068 [Abstract] [Full Text]
Milne, J. L.S., Caterina, M. J., Devreotes, P. N. (1997). Random Mutagenesis of the cAMP Chemoattractant Receptor, cAR1, of Dictyostelium. EVIDENCE FOR MULTIPLE STATES OF ACTIVATION. J. Biol. Chem. 272: 2069-2076 [Abstract] [Full Text]
Sager, B M (1996). Propagation of traveling waves in excitable media.. Genes Dev. 10: 2237-2250
Parent, C. A., Devreotes, P. N. (1996). Constitutively Active Adenylyl Cyclase Mutant Requires Neither G Proteins nor Cytosolic Regulators. J. Biol. Chem. 271: 18333-18336 [Abstract] [Full Text]
Maeda, M., Aubry, L., Insall, R., Gaskins, C., Devreotes, P. N., Firtel, R. A. (1996). Seven Helix Chemoattractant Receptors Transiently Stimulate Mitogen-activated Protein Kinase in Dictyostelium. J. Biol. Chem. 271: 3351-3354 [Abstract] [Full Text]
Parent, C. A., Devreotes, P. N. (1995). Isolation of Inactive and G Protein-resistant Adenylyl Cyclase Mutants Using Random Mutagenesis. J. Biol. Chem. 270: 22693-22696 [Abstract] [Full Text]
van Es, S., Weening, K. E., Devreotes, P. N. (2001). The Protein Kinase YakA Regulates G-protein-linked Signaling Responses during Growth and Development of Dictyostelium. J. Biol. Chem. 276: 30761-30765 [Abstract] [Full Text]
Roelofs, J., Loovers, H. M., Van Haastert, P. J. M. (2001). GTPgamma S Regulation of a 12-Transmembrane Guanylyl Cyclase Is Retained after Mutation to an Adenylyl Cyclase. J. Biol. Chem. 276: 40740-40745 [Abstract] [Full Text]