p120, a p120-related protein (p100), and the cadherin/catenin complex

doi:10.1083/jcb.130.2.369

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 4156K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Staddon, J. M.
	Articles by Rubin, L. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Staddon, J. M.
	Articles by Rubin, L. L.


Social Bookmarking

	What's this?

ARTICLES

p120, a p120-related protein (p100), and the cadherin/catenin complex

JM Staddon, C Smales, C Schulze, FS Esch and LL Rubin
Eisai London Research Laboratories Ltd., University College London, United Kingdom.

Cadherins and catenins play an important role in cell-cell adhesion. Two of the catenins, beta and gamma, are members of a group of proteins that contains a repeating amino acid motif originally described for the Drosophila segment polarity gene armadillo. Another member of this group is a 120-kD protein termed p120, originally identified as a substrate of the tyrosine kinase pp60src. In this paper, we show that endothelial and epithelial cells express p120 and p100, a 100-kD, p120- related protein. Peptide sequencing of p100 establishes it as highly related to p120. p120 and p100 both appear associated with the cadherin/catenin complex, but independent p120/catenin and p100/catenin complexes can be isolated. This association is shown by coimmunoprecipitation of cadherins and catenins with an anti-p120/p100 antibody, and of p120/p100 with cadherin or catenin antibodies. Immunocytochemical analysis with a p120-specific antibody reveals junctional colocalization of p120 and beta-catenin in epithelial cells. Catenins and p120/p100 also colocalize in endothelial and epithelial cells in culture and in tissue sections. The cellular content of p120/p100 and beta-catenin is similar in MDCK cells, but only approximately 20% of the p120/p100 pool associates with the cadherin/catenin complex. Our data provide further evidence for interactions among the different arm proteins and suggest that p120/p100 may participate in regulating the function of cadherins and, thereby, other processes influenced by cell-cell adhesion.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Taulet, N., Comunale, F., Favard, C., Charrasse, S., Bodin, S., Gauthier-Rouviere, C. (2009). N-cadherin/p120 Catenin Association at Cell-Cell Contacts Occurs in Cholesterol-rich Membrane Domains and Is Required for RhoA Activation and Myogenesis. J. Biol. Chem. 284: 23137-23145 [Abstract] [Full Text]
Ogden, S. R., Wroblewski, L. E., Weydig, C., Romero-Gallo, J., O'Brien, D. P., Israel, D. A., Krishna, U. S., Fingleton, B., Reynolds, A. B., Wessler, S., Peek, R. M. Jr (2008). p120 and Kaiso Regulate Helicobacter pylori-induced Expression of Matrix Metalloproteinase-7. Mol. Biol. Cell 19: 4110-4121 [Abstract] [Full Text]
Taniuchi, K., Nakagawa, H., Hosokawa, M., Nakamura, T., Eguchi, H., Ohigashi, H., Ishikawa, O., Katagiri, T., Nakamura, Y. (2005). Overexpressed P-Cadherin/CDH3 Promotes Motility of Pancreatic Cancer Cells by Interacting with p120ctn and Activating Rho-Family GTPases. Cancer Res. 65: 3092-3099 [Abstract] [Full Text]
Kausalya, P. J., Phua, D. C.Y., Hunziker, W. (2004). Association of ARVCF with Zonula Occludens (ZO)-1 and ZO-2: Binding to PDZ-Domain Proteins and Cell-Cell Adhesion Regulate Plasma Membrane and Nuclear Localization of ARVCF. Mol. Biol. Cell 15: 5503-5515 [Abstract] [Full Text]
Franz, C. M., Ridley, A. J. (2004). p120 Catenin Associates with Microtubules: INVERSE RELATIONSHIP BETWEEN MICROTUBULE BINDING AND RHO GTPase REGULATION. J. Biol. Chem. 279: 6588-6594 [Abstract] [Full Text]
Roczniak-Ferguson, A., Reynolds, A. B. (2003). Regulation of p120-catenin nucleocytoplasmic shuttling activity. J. Cell Sci. 116: 4201-4212 [Abstract] [Full Text]
Hatzfeld, M., Green, K. J., Sauter, H. (2003). Targeting of p0071 to desmosomes and adherens junctions is mediated by different protein domains. J. Cell Sci. 116: 1219-1233 [Abstract] [Full Text]
Ireton, R. C., Davis, M. A., van Hengel, J., Mariner, D. J., Barnes, K., Thoreson, M. A., Anastasiadis, P. Z., Matrisian, L., Bundy, L. M., Sealy, L., Gilbert, B., van Roy, F., Reynolds, A. B. (2002). A novel role for p120 catenin in E-cadherin function. JCB 159: 465-476 [Abstract] [Full Text]
van Buul, J. D., Voermans, C., van den Berg, V., Anthony, E. C., Mul, F. P. J., van Wetering, S., van der Schoot, C. E., Hordijk, P. L. (2002). Migration of Human Hematopoietic Progenitor Cells Across Bone Marrow Endothelium Is Regulated by Vascular Endothelial Cadherin. J. Immunol. 168: 588-596 [Abstract] [Full Text]
Aho, S., Levansuo, L., Montonen, O., Kari, C., Rodeck, U., Uitto, J. (2002). Specific sequences in p120ctn determine subcellular distribution of its multiple isoforms involved in cellular adhesion of normal and malignant epithelial cells. J. Cell Sci. 115: 1391-1402 [Abstract] [Full Text]
Montonen, O., Aho, M., Uitto, J., Aho, S. (2001). Tissue Distribution and Cell Type-specific Expression of p120ctn Isoforms. J. Histochem. Cytochem. 49: 1487-1496 [Abstract] [Full Text]
Xu, X., Li, W.E.I., Huang, G.Y., Meyer, R., Chen, T., Luo, Y., Thomas, M.P., Radice, G.L., Lo, C.W. (2001). Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions. JCB 154: 217-230 [Abstract] [Full Text]
Ozawa, M, Ohkubo, T (2001). Tyrosine phosphorylation of p120(ctn) in v-Src transfected L cells depends on its association with E-cadherin and reduces adhesion activity. J. Cell Sci. 114: 503-512 [Abstract]
Grosheva, I, Shtutman, M, Elbaum, M, Bershadsky, A. (2001). p120 catenin affects cell motility via modulation of activity of Rho-family GTPases: a link between cell-cell contact formation and regulation of cell locomotion. J. Cell Sci. 114: 695-707 [Abstract]
Sasaki, C. Y., Lin, H., Morin, P. J., Longo, D. L. (2000). Truncation of the Extracellular Region Abrogrates Cell Contact but Retains the Growth-suppressive Activity of E-cadherin. Cancer Res. 60: 7057-7065 [Abstract] [Full Text]
Noren, N. K., Liu, B. P., Burridge, K., Kreft, B. (2000). P120 Catenin Regulates the Actin Cytoskeleton via Rho Family Gtpases. JCB 150: 567-580 [Abstract] [Full Text]
Ohene-Abuakwa, Y., Pignatelli, M. (2000). Adhesion Molecules as Diagnostic Tools in Tumor Pathology. INT J SURG PATHOL 8: 191-200 [Abstract]
Zondag, G. C. M., Reynolds, A. B., Moolenaar, W. H. (2000). Receptor Protein-tyrosine Phosphatase RPTP{micro} Binds to and Dephosphorylates the Catenin p120ctn. J. Biol. Chem. 275: 11264-11269 [Abstract] [Full Text]
Kaufmann, U, Zuppinger, C, Waibler, Z, Rudiger, M, Urbich, C, Martin, B, Jockusch, B., Eppenberger, H, Starzinski-Powitz, A (2000). The armadillo repeat region targets ARVCF to cadherin-based cellular junctions. J. Cell Sci. 113: 4121-4135 [Abstract]
Allport, J. R., Muller, W. A., Luscinskas, F. W. (2000). Monocytes Induce Reversible Focal Changes in Vascular Endothelial Cadherin Complex during Transendothelial Migration under Flow. JCB 148: 203-216 [Abstract] [Full Text]
Thoreson, M. A., Anastasiadis, P. Z., Daniel, J. M., Ireton, R. C., Wheelock, M. J., Johnson, K. R., Hummingbird, D. K., Reynolds, A. B. (2000). Selective Uncoupling of P120ctn from E-Cadherin Disrupts Strong Adhesion. JCB 148: 189-202 [Abstract] [Full Text]
Lambert, M, Padilla, F, Mege, R. (2000). Immobilized dimers of N-cadherin-Fc chimera mimic cadherin-mediated cell contact formation: contribution of both outside-in and inside-out signals. J. Cell Sci. 113: 2207-2219 [Abstract]
Anastasiadis, P., Reynolds, A. (2000). The p120 catenin family: complex roles in adhesion, signaling and cancer. J. Cell Sci. 113: 1319-1334 [Abstract]
Ohkubo, T., Ozawa, M. (1999). p120ctn Binds to the Membrane-proximal Region of the E-cadherin Cytoplasmic Domain and Is Involved in Modulation of Adhesion Activity. J. Biol. Chem. 274: 21409-21415 [Abstract] [Full Text]
Aono, S., Nakagawa, S., Reynolds, A. B., Takeichi, M. (1999). p120ctn Acts as an Inhibitory Regulator of Cadherin Function in Colon Carcinoma Cells. JCB 145: 551-562 [Abstract] [Full Text]
Daniel, J. M., Reynolds, A. B. (1999). The Catenin p120ctn Interacts with Kaiso, a Novel BTB/POZ Domain Zinc Finger Transcription Factor. Mol. Cell. Biol. 19: 3614-3623 [Abstract] [Full Text]
Pötter, E., Bergwitz, C., Brabant, G. (1999). The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues. Endocr. Rev. 20: 207-239 [Abstract] [Full Text]
Lu, Q., Paredes, M., Medina, M., Zhou, J., Cavallo, R., Peifer, M., Orecchio, L., Kosik, K. S. (1999). {delta}-catenin, an Adhesive Junction-associated Protein Which Promotes Cell Scattering. JCB 144: 519-532 [Abstract] [Full Text]
YEAMAN, C., GRINDSTAFF, K. K., NELSON, W. J. (1999). New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity. Physiol. Rev. 79: 73-98 [Abstract] [Full Text]
Rosato, R., Veltmaat, J. M., Groffen, J., Heisterkamp, N. (1998). Involvement of the Tyrosine Kinase Fer in Cell Adhesion. Mol. Cell. Biol. 18: 5762-5770 [Abstract] [Full Text]
Ozawa, M., Kemler, R. (1998). The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion Activity. JCB 142: 1605-1613 [Abstract] [Full Text]
Adamson, R. H., Liu, B., Fry, G. N., Rubin, L. L., Curry, F. E. (1998). Microvascular permeability and number of tight junctions are modulated by cAMP. Am. J. Physiol. Heart Circ. Physiol. 274: H1885-H1894 [Abstract] [Full Text]
Yap, A. S., Niessen, C. M., Gumbiner, B. M. (1998). The Juxtamembrane Region of the Cadherin Cytoplasmic Tail Supports Lateral Clustering, Adhesive Strengthening, and Interaction with p120ctn. JCB 141: 779-789 [Abstract] [Full Text]
Ozawa, M., Kemler, R. (1998). Altered Cell Adhesion Activity by Pervanadate Due to the Dissociation of alpha -Catenin from the E-Cadherin�Catenin Complex. J. Biol. Chem. 273: 6166-6170 [Abstract] [Full Text]
Ratcliffe, M. J., Rubin, L. L., Staddon, J. M. (1997). Dephosphorylation of the Cadherin-associated p100/p120 Proteins in Response to Activation of Protein Kinase C in Epithelial Cells. J. Biol. Chem. 272: 31894-31901 [Abstract] [Full Text]
Allport, J. R., Ding, H., Collins, T., Gerritsen, M. E., Luscinskas, F. W. (1997). Endothelial-dependent Mechanisms Regulate Leukocyte Transmigration: A Process Involving the Proteasome and Disruption of the Vascular Endothelial-Cadherin Complex at Endothelial Cell-to-Cell Junctions. JEM 186: 517-527 [Abstract] [Full Text]
Papkoff, J., Papkoff, J. (1997). Regulation of Complexed and Free Catenin Pools by Distinct Mechanisms. DIFFERENTIAL EFFECTS OF Wnt-1 AND v-Src. J. Biol. Chem. 272: 4536-4543 [Abstract] [Full Text]
Lampugnani, M., Corada, M, Andriopoulou, P, Esser, S, Risau, W, Dejana, E (1997). Cell confluence regulates tyrosine phosphorylation of adherens junction components in endothelial cells. J. Cell Sci. 110: 2065-2077 [Abstract]
Hirase, T, Staddon, J., Saitou, M, Ando-Akatsuka, Y, Itoh, M, Furuse, M, Fujimoto, K, Tsukita, S, Rubin, L. (1997). Occludin as a possible determinant of tight junction permeability in endothelial cells. J. Cell Sci. 110: 1603-1613 [Abstract]
Miller, J R, Moon, R T (1996). Signal transduction through beta-catenin and specification of cell fate during embryogenesis.. Genes Dev. 10: 2527-2539
Rabiet, M.-J., Plantier, J.-L., Rival, Y., Genoux, Y., Lampugnani, M.-G., Dejana, E. (1996). Thrombin-Induced Increase in Endothelial Permeability Is Associated With Changes in Cell-to-Cell Junction Organization. Arterioscler. Thromb. Vasc. Bio. 16: 488-496 [Abstract] [Full Text]
Troyanovsky, R., Chitaev, N., Troyanovsky, S. (1996). Cadherin binding sites of plakoglobin: localization, specificity and role in targeting to adhering junctions. J. Cell Sci. 109: 3069-3078 [Abstract]
Hatzfeld, M, Nachtsheim, C (1996). Cloning and characterization of a new armadillo family member, p0071, associated with the junctional plaque: evidence for a subfamily of closely related proteins. J. Cell Sci. 109: 2767-2778 [Abstract]
Corvera, S., DiBonaventura, C., Shpetner, H. S. (2000). Cell Confluence-dependent Remodeling of Endothelial Membranes Mediated by Cholesterol. J. Biol. Chem. 275: 31414-31421 [Abstract] [Full Text]
Deguchi, M., Iizuka, T., Hata, Y., Nishimura, W., Hirao, K., Yao, I., Kawabe, H., Takai, Y. (2000). PAPIN. A NOVEL MULTIPLE PSD-95/Dlg-A/ZO-1 PROTEIN INTERACTING WITH NEURAL PLAKOPHILIN-RELATED Armadillo REPEAT PROTEIN/delta -CATENIN AND p0071. J. Biol. Chem. 275: 29875-29880 [Abstract] [Full Text]
Huber, A. H., Stewart, D. B., Laurents, D. V., Nelson, W. J., Weis, W. I. (2001). The Cadherin Cytoplasmic Domain Is Unstructured in the Absence of beta -Catenin. A POSSIBLE MECHANISM FOR REGULATING CADHERIN TURNOVER. J. Biol. Chem. 276: 12301-12309 [Abstract] [Full Text]
Horikawa, K., Takeichi, M. (2001). Requirement of the juxtamembrane domain of the cadherin cytoplasmic tail for morphogenetic cell rearrangement during myotome development. JCB 155: 1297-1306 [Abstract] [Full Text]