Reduced contact-inhibition and substratum adhesion in epithelial cells expressing GlcNAc-transferase V

doi:10.1083/jcb.130.2.383

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Reduced contact-inhibition and substratum adhesion in epithelial cells expressing GlcNAc-transferase V

M Demetriou, IR Nabi, M Coppolino, S Dedhar and JW Dennis
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario.

Malignant transformation of fibroblast and epithelial cells is accompanied by increased beta 1-6 N-acetylglucosaminyltransferase V (GlcNAc-TV) activity, a Golgi N-linked oligosaccharide processing enzyme. Herein, we report that expression of GlcNAc-TV in Mv1Lu cells, an immortalized lung epithelial cell line results in loss of contact- inhibition of cell growth, an effect that was blocked by swainsonine, an inhibitor of Golgi processing enzyme alpha-mannosidase II. In serum- deprived and high density monolayer cultures, the GlcNAc-TV transfectants formed foci, maintained microfilaments characteristic of proliferating cells, and also experienced accelerated cell death by apoptosis. Injection of the GlcNAc-TV transfectants into nude mice produced a 50% incidence of benign tumors, and progressively growing tumors in 2:12 mice with a latency of 6 mo, while no growth was observed in mice injected with control cells. In short term adhesion assays, the GlcNAc-TV expressing cells were less adhesive on surfaces coated with fibronectin and collagen type IV, but no changes were observed in levels of cell surface alpha 5 beta 1 or alpha v beta 3 integrins. The larger apparent molecular weights of the LAMP-2 glycoprotein and integrin glycoproteins alpha 5, alpha v and beta 1 in the transfected cells indicates that their oligosaccharide chains are substrates for GlcNAc-TV. The results suggest that beta 1-6GlcNAc branching of N-linked oligosaccharides contributes directly to relaxed growth controls and reduce substratum adhesion in premalignant epithelial cells.
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Sasai, K., Ikeda, Y., Fujii, T., Tsuda, T., Taniguchi, N. (2002). UDP-GlcNAc concentration is an important factor in the biosynthesis of {beta}1,6-branched oligosaccharides: regulation based on the kinetic properties of N-acetylglucosaminyltransferase V. Glycobiology 12: 119-127 [Abstract] [Full Text]
Skinner, M. A., Wildeman, A. G. (2001). Suppression of Tumor-related Glycosylation of Cell Surface Receptors by the 16-kDa Membrane Subunit of Vacuolar H+-ATPase. J. Biol. Chem. 276: 48451-48457 [Abstract] [Full Text]
Chakraborty, A. K., Pawelek, J., Ikeda, Y., Miyoshi, E., Kolesnikova, N., Funasaka, Y., Ichihashi, M., Taniguchi, N. (2001). Fusion Hybrids with Macrophage and Melanoma Cells Up-Regulate N-Acetylglucosaminyltransferase V, {beta}1-6 Branching, and Metastasis. Cell Growth Differ. 12: 623-630 [Abstract] [Full Text]
Yamamoto, H., Oviedo, A., Sweeley, C., Saito, T., Moskal, J. R. (2001). {alpha}2,6-Sialylation of Cell-Surface N-Glycans Inhibits Glioma Formation in Vivo. Cancer Res. 61: 6822-6829 [Abstract] [Full Text]
Korczak, B., Le, T., Elowe, S., Datti, A., Dennis, J. W. (2000). Minimal catalytic domain of N-acetylglucosaminyltransferase V. Glycobiology 10: 595-599 [Abstract] [Full Text]
Murata, K., Miyoshi, E., Kameyama, M., Ishikawa, O., Kabuto, T., Sasaki, Y., Hiratsuka, M., Ohigashi, H., Ishiguro, S., Ito, S., Honda, H., Takemura, F., Taniguchi, N., Imaoka, S. (2000). Expression of N-Acetylglucosaminyltransferase V in Colorectal Cancer Correlates with Metastasis and Poor Prognosis. Clin. Cancer Res. 6: 1772-1777 [Abstract] [Full Text]
Petretti, T, Kemmner, W, Schulze, B, Schlag, P M (2000). Altered mRNA expression of glycosyltransferases in human colorectal carcinomas and liver metastases. Gut 46: 359-366 [Abstract] [Full Text]
Yamamoto, H., Swoger, J., Greene, S., Saito, T., Hurh, J., Sweeley, C., Leestma, J., Mkrdichian, E., Cerullo, L., Nishikawa, A., Ihara, Y., Taniguchi, N., Moskal, J. R. (2000). {beta}1,6-N-Acetylglucosamine-bearing N-Glycans in Human Gliomas: Implications for a Role in Regulating Invasivity. Cancer Res. 60: 134-142 [Abstract] [Full Text]
Hariri, M., Millane, G., Guimond, M.-P., Guay, G., Dennis, J. W., Nabi, I. R. (2000). Biogenesis of Multilamellar Bodies via Autophagy. Mol. Biol. Cell 11: 255-268 [Abstract] [Full Text]
Ko, J. H., Miyoshi, E., Noda, K., Ekuni, A., Kang, R., Ikeda, Y., Taniguchi, N. (1999). Regulation of the GnT-V Promoter by Transcription Factor Ets-1 in Various Cancer Cell Lines. J. Biol. Chem. 274: 22941-22948 [Abstract] [Full Text]
Ujita, M., McAuliffe, J., Hindsgaul, O., Sasaki, K., Fukuda, M. N., Fukuda, M. (1999). Poly-N-acetyllactosamine Synthesis in Branched N-Glycans Is Controlled by Complemental Branch Specificity of i-Extension Enzyme and beta 1,4-Galactosyltransferase I. J. Biol. Chem. 274: 16717-16726 [Abstract] [Full Text]
Miyoshi, E., Noda, K., Ko, J. H., Ekuni, A., Kitada, T., Uozumi, N., Ikeda, Y., Matsuura, N., Sasaki, Y., Hayashi, N., Hori, M., Taniguchi, N. (1999). Overexpression of {{alpha}}1-6 Fucosyltransferase in Hepatoma Cells Suppresses Intrahepatic Metastasis after Splenic Injection in Athymic Mice. Cancer Res. 59: 2237-2243 [Abstract] [Full Text]
Nemansky, M., Thotakura, N. R., Lyons, C. D., Ye, S., Reinhold, B. B., Reinhold, V. N., Blithe, D. L. (1998). Developmental Changes in the Glycosylation of Glycoprotein Hormone Free alpha �Subunit during Pregnancy. J. Biol. Chem. 273: 12068-12076 [Abstract] [Full Text]
Sasaki, K., Kurata-Miura, K., Ujita, M., Angata, K., Nakagawa, S., Sekine, S., Nishi, T., Fukuda, M. (1997). Expression cloning of cDNA encoding a human beta -1,3-N-acetylglucosaminyltransferase that is essential for�poly-N-acetyllactosamine�synthesis. Proc. Natl. Acad. Sci. USA 94: 14294-14299 [Abstract] [Full Text]
Yan, Z., Chen, M.-x., Perucho, M., Friedman, E. (1997). Oncogenic Ki-ras but Not Oncogenic Ha-ras Blocks Integrin beta 1-Chain Maturation in Colon Epithelial Cells. J. Biol. Chem. 272: 30928-30936 [Abstract] [Full Text]
Buckhaults, P., Chen, L., Fregien, N., Pierce, M. (1997). Transcriptional Regulation of N-Acetylglucosaminyltransferase V by the src Oncogene. J. Biol. Chem. 272: 19575-19581 [Abstract] [Full Text]
Weinstein, J., Sundaram, S., Wang, X., Delgado, D., Basu, R., Stanley, P. (1996). A Point Mutation Causes Mistargeting of Golgi GlcNAc-TV in the Lec4A Chinese Hamster Ovary Glycosylation Mutant. J. Biol. Chem. 271: 27462-27469 [Abstract] [Full Text]
Kang, R., Saito, H., Ihara, Y., Miyoshi, E., Koyama, N., Sheng, Y., Taniguchi, N. (1996). Transcriptional Regulation of the N-Acetylglucosaminyltransferase V Gene in Human Bile Duct Carcinoma Cells (HuCC-T1) Is Mediated by Ets-1. J. Biol. Chem. 271: 26706-26712 [Abstract] [Full Text]
Yoshimura, M., Ihara, Y., Matsuzawa, Y., Taniguchi, N. (1996). Aberrant Glycosylation of E-cadherin Enhances Cell-Cell Binding to Suppress Metastasis. J. Biol. Chem. 271: 13811-13815 [Abstract] [Full Text]
Sasai, K., Ikeda, Y., Tsuda, T., Ihara, H., Korekane, H., Shiota, K., Taniguchi, N. (2001). The Critical Role of the Stem Region as a Functional Domain Responsible for the Oligomerization and Golgi Localization of N-Acetylglucosaminyltransferase V. THE INVOLVEMENT OF A DOMAIN HOMOPHILIC INTERACTION. J. Biol. Chem. 276: 759-765 [Abstract] [Full Text]