Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis

doi:10.1083/jcb.130.3.651

This Article

	Full Text (PDF, 2315K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by McCollum, D.
	Articles by Gould, K. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by McCollum, D.
	Articles by Gould, K. L.


Social Bookmarking

	What's this?

ARTICLES

Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis

D McCollum, MK Balasubramanian, LE Pelcher, SM Hemmingsen and KL Gould
Howard Hughes Medical Institute, Vanderbilt University, Nashville, Tennessee 37232, USA.

Schizosaccharomyces pombe cells divide by medial fission. One class of cell division mutants (cdc), the late septation mutants, defines four genes: cdc3, cdc4, cdc8, and cdc12 (Nurse, P., P. Thuriaux, and K. Nasmyth. 1976. Mol. & Gen. Genet. 146:167-178). We have cloned and characterized the cdc4 gene and show that the predicted gene product. Cdc4p, is a 141-amino acid polypeptide that is similar in sequence to EF-hand proteins including myosin light chains, calmodulin, and troponin C. Two temperature-sensitive lethal alleles, cdc4-8 and cdc4- 31, accumulate multiple nuclei and multiple improper F-actin rings and septa but fail to complete cytokinesis. Deletion of cdc4 also results in a lethal terminal phenotype characterized by multinucleate, elongated cells that fail to complete cytokinesis. Sequence comparisons suggest that Cdc4p may be a member of a new class of EF-hand proteins. Cdc4p localizes to a ringlike structure in the medial region of cells undergoing cytokinesis. Thus, Cdc4p appears to be an essential component of the F-actin contractile ring. We find that Cdc4 protein forms a complex with a 200-kD protein which can be cross-linked to UTP, a property common to myosin heavy chains. Together these results suggest that Cdc4p may be a novel myosin light chain.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Sladewski, T. E., Previs, M. J., Lord, M. (2009). Regulation of Fission Yeast Myosin-II Function and Contractile Ring Dynamics by Regulatory Light-Chain and Heavy-Chain Phosphorylation. Mol. Biol. Cell 20: 3941-3952 [Abstract] [Full Text]
Satoh, R., Morita, T., Takada, H., Kita, A., Ishiwata, S., Doi, A., Hagihara, K., Taga, A., Matsumura, Y., Tohda, H., Sugiura, R. (2009). Role of the RNA-binding Protein Nrd1 and Pmk1 Mitogen-activated Protein Kinase in the Regulation of Myosin mRNA Stability in Fission Yeast. Mol. Biol. Cell 20: 2473-2485 [Abstract] [Full Text]
Huang, Y., Yan, H., Balasubramanian, M. K. (2008). Assembly of normal actomyosin rings in the absence of Mid1p and cortical nodes in fission yeast. JCB 183: 979-988 [Abstract] [Full Text]
Hachet, O., Simanis, V. (2008). Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis. Genes Dev. 22: 3205-3216 [Abstract] [Full Text]
Yan, H., Ge, W., Chew, T. G., Chow, J. Y., McCollum, D., Neiman, A. M., Balasubramanian, M. K. (2008). The Meiosis-Specific Sid2p-related Protein Slk1p Regulates Forespore Membrane Assembly in Fission Yeast. Mol. Biol. Cell 19: 3676-3690 [Abstract] [Full Text]
Pinar, M., Coll, P. M., Rincon, S. A., Perez, P. (2008). Schizosaccharomyces pombe Pxl1 Is a Paxillin Homologue That Modulates Rho1 Activity and Participates in Cytokinesis. Mol. Biol. Cell 19: 1727-1738 [Abstract] [Full Text]
Ge, W., Balasubramanian, M. K. (2008). Pxl1p, a Paxillin-related Protein, Stabilizes the Actomyosin Ring during Cytokinesis in Fission Yeast. Mol. Biol. Cell 19: 1680-1692 [Abstract] [Full Text]
Vjestica, A., Tang, X.-Z., Oliferenko, S. (2008). The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast. Mol. Biol. Cell 19: 1125-1138 [Abstract] [Full Text]
Kamasaki, T., Osumi, M., Mabuchi, I. (2007). Three-dimensional arrangement of F-actin in the contractile ring of fission yeast. JCB 178: 765-771 [Abstract] [Full Text]
Wachtler, V., Huang, Y., Karagiannis, J., Balasubramanian, M. K. (2006). Cell Cycle-dependent Roles for the FCH-Domain Protein Cdc15p in Formation of the Actomyosin Ring in Schizosaccharomyces pombe. Mol. Biol. Cell 17: 3254-3266 [Abstract] [Full Text]
Nakano, K., Mabuchi, I. (2006). Actin-depolymerizing Protein Adf1 Is Required for Formation and Maintenance of the Contractile Ring during Cytokinesis in Fission Yeast. Mol. Biol. Cell 17: 1933-1945 [Abstract] [Full Text]
Lord, M., Laves, E., Pollard, T. D. (2005). Cytokinesis Depends on the Motor Domains of Myosin-II in Fission Yeast but Not in Budding Yeast. Mol. Biol. Cell 16: 5346-5355 [Abstract] [Full Text]
Bimbo, A., Liu, J., Balasubramanian, M. K. (2005). Roles of Pdk1p, a Fission Yeast Protein Related to Phosphoinositide-dependent Protein Kinase, in the Regulation of Mitosis and Cytokinesis. Mol. Biol. Cell 16: 3162-3175 [Abstract] [Full Text]
Mishra, M., D'souza, V. M., Chang, K. C., Huang, Y., Balasubramanian, M. K. (2005). Hsp90 Protein in Fission Yeast Swo1p and UCS Protein Rng3p Facilitate Myosin II Assembly and Function. Eukaryot Cell 4: 567-576 [Abstract] [Full Text]
Yamashita, A., Sato, M., Fujita, A., Yamamoto, M., Toda, T. (2005). The Roles of Fission Yeast Ase1 in Mitotic Cell Division, Meiotic Nuclear Oscillation, and Cytokinesis Checkpoint Signaling. Mol. Biol. Cell 16: 1378-1395 [Abstract] [Full Text]
Celton-Morizur, S., Bordes, N., Fraisier, V., Tran, P. T., Paoletti, A. (2004). C-Terminal Anchoring of mid1p to Membranes Stabilizes Cytokinetic Ring Position in Early Mitosis in Fission Yeast. Mol. Cell. Biol. 24: 10621-10635 [Abstract] [Full Text]
Lord, M., Pollard, T. D. (2004). UCS protein Rng3p activates actin filament gliding by fission yeast myosin-II. JCB 167: 315-325 [Abstract] [Full Text]
Mishra, M., Karagiannis, J., Trautmann, S., Wang, H., McCollum, D., Balasubramanian, M. K. (2004). The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe. J. Cell Sci. 117: 3897-3910 [Abstract] [Full Text]
Luo, J., Vallen, E. A., Dravis, C., Tcheperegine, S. E., Drees, B., Bi, E. (2004). Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. JCB 165: 843-855 [Abstract] [Full Text]
Motegi, F., Mishra, M., Balasubramanian, M. K., Mabuchi, I. (2004). Myosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast. JCB 165: 685-695 [Abstract] [Full Text]
Hirota, K., Tanaka, K., Ohta, K., Yamamoto, M. (2003). Gef1p and Scd1p, the Two GDP-GTP Exchange Factors for Cdc42p, Form a Ring Structure that Shrinks during Cytokinesis in Schizosaccharomyces pombe. Mol. Biol. Cell 14: 3617-3627 [Abstract] [Full Text]
Berlin, A., Paoletti, A., Chang, F. (2003). Mid2p stabilizes septin rings during cytokinesis in fission yeast. JCB 160: 1083-1092 [Abstract] [Full Text]
Salus, S. S., Demeter, J., Sazer, S. (2002). The Ran GTPase System in Fission Yeast Affects Microtubules and Cytokinesis in Cells That Are Competent for Nucleocytoplasmic Protein Transport. Mol. Cell. Biol. 22: 8491-8505 [Abstract] [Full Text]
Karagiannis, J., Oulton, R., Young, P. G. (2002). The Scw1 RNA-Binding Domain Protein Regulates Septation and Cell-Wall Structure in Fission Yeast. Genetics 162: 45-58 [Abstract] [Full Text]
Fujita, M., Sugiura, R., Lu, Y., Xu, L., Xia, Y., Shuntoh, H., Kuno, T. (2002). Genetic Interaction Between Calcineurin and Type 2 Myosin and Their Involvement in the Regulation of Cytokinesis and Chloride Ion Homeostasis in Fission Yeast. Genetics 161: 971-981 [Abstract] [Full Text]
Guertin, D. A., Trautmann, S., McCollum, D. (2002). Cytokinesis in Eukaryotes. Microbiol. Mol. Biol. Rev. 66: 155-178 [Abstract] [Full Text]
Rajagopalan, S., Balasubramanian, M. K. (2002). Schizosaccharomyces pombe Bir1p, a Nuclear Protein That Localizes to Kinetochores and the Spindle Midzone, Is Essential for Chromosome Condensation and Spindle Elongation During Mitosis. Genetics 160: 445-456 [Abstract] [Full Text]
Arai, R., Mabuchi, I. (2002). F-actin ring formation and the role of F-actin cables in the fission yeast Schizosaccharomyces pombe. J. Cell Sci. 115: 887-898 [Abstract] [Full Text]
Mulvihill, D. P., Barretto, C., Hyams, J. S. (2001). Localization of Fission Yeast Type II Myosin, Myo2, to the Cytokinetic Actin Ring Is Regulated by Phosphorylation of a C-Terminal Coiled-Coil Domain and Requires a Functional Septation Initiation Network. Mol. Biol. Cell 12: 4044-4053 [Abstract] [Full Text]
Nakano, K., Satoh, K., Morimatsu, A., Ohnuma, M., Mabuchi, I. (2001). Interactions among a Fimbrin, a Capping Protein, and an Actin-depolymerizing Factor in Organization of the Fission Yeast Actin Cytoskeleton. Mol. Biol. Cell 12: 3515-3526 [Abstract] [Full Text]
Motegi, F., Arai, R., Mabuchi, I. (2001). Identification of Two Type V Myosins in Fission Yeast, One of Which Functions in Polarized Cell Growth and Moves Rapidly in the Cell. Mol. Biol. Cell 12: 1367-1380 [Abstract] [Full Text]
Wu, J.-Q., Bähler, J., Pringle, J. R. (2001). Roles of a Fimbrin and an {alpha}-Actinin-like Protein in Fission Yeast Cell Polarization and Cytokinesis. Mol. Biol. Cell 12: 1061-1077 [Abstract] [Full Text]
Robinson, D. N., Spudich, J. A. (2000). Dynacortin, a Genetic Link between Equatorial Contractility and Global Shape Control Discovered by Library Complementation of a Dictyostelium discoideum Cytokinesis Mutant. JCB 150: 823-838 [Abstract] [Full Text]
Cullen, C. F., May, K. M., Hagan, I. M., Glover, D. M., Ohkura, H. (2000). A New Genetic Method for Isolating Functionally Interacting Genes: High plo1+-Dependent Mutants and Their Suppressors Define Genes in Mitotic and Septation Pathways in Fission Yeast. Genetics 155: 1521-1534 [Abstract] [Full Text]
Paoletti, A., Chang, F. (2000). Analysis of mid1p, a Protein Required for Placement of the Cell Division Site, Reveals a Link between the Nucleus and the Cell Surface in Fission Yeast. Mol. Biol. Cell 11: 2757-2773 [Abstract] [Full Text]
Chang, L., Gould, K. L. (2000). Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast. Proc. Natl. Acad. Sci. USA 97: 5249-5254 [Abstract] [Full Text]
Vallen, E. A., Caviston, J., Bi, E. (2000). Roles of Hof1p, Bni1p, Bnr1p, and Myo1p in Cytokinesis in Saccharomyces cerevisiae. Mol. Biol. Cell 11: 593-611 [Abstract] [Full Text]
Le Goff, X, Motegi, F, Salimova, E, Mabuchi, I, Simanis, V (2000). The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p. J. Cell Sci. 113: 4157-4163 [Abstract]
Wong, K., Naqvi, N., Iino, Y, Yamamoto, M, Balasubramanian, M. (2000). Fission yeast Rng3p: an UCS-domain protein that mediates myosin II assembly during cytokinesis. J. Cell Sci. 113: 2421-2432 [Abstract]
Motegi, F, Nakano, K, Mabuchi, I (2000). Molecular mechanism of myosin-II assembly at the division site in Schizosaccharomyces pombe. J. Cell Sci. 113: 1813-1825 [Abstract]
Liu, J., Wang, H., McCollum, D., Balasubramanian, M. K. (1999). Drc1p/Cps1p, a 1,3-{beta}-Glucan Synthase Subunit, Is Essential for Division Septum Assembly in Schizosaccharomyces pombe. Genetics 153: 1193-1203 [Abstract] [Full Text]
McCollum, D., Feoktistova, A., Gould, K. L. (1999). Phosphorylation of the Myosin-II Light Chain Does Not Regulate the Timing of Cytokinesis in Fission Yeast. J. Biol. Chem. 274: 17691-17695 [Abstract] [Full Text]
Bahler, J., Steever, A. B., Wheatley, S., Wang, Y.-l., Pringle, J. R., Gould, K. L., McCollum, D. (1998). Role of Polo Kinase and Mid1p in Determining the Site of Cell Division in Fission Yeast. JCB 143: 1603-1616 [Abstract] [Full Text]
Demeter, J., Sazer, S. (1998). imp2, a New Component of the Actin Ring in the Fission Yeast Schizosaccharomyces pombe. JCB 143: 415-427 [Abstract] [Full Text]
Balasubramanian, M. K., McCollum, D., Chang, L., Wong, K. C. Y., Naqvi, N. I., He, X., Sazer, S., Gould, K. L. (1998). Isolation and Characterization of New Fission Yeast Cytokinesis Mutants. Genetics 149: 1265-1275 [Abstract] [Full Text]
Bähler, J., Pringle, J. R. (1998). Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and�cytokinesis. Genes Dev. 12: 1356-1370 [Abstract] [Full Text]
Grallert, A., Grallert, B., Ribar, B., Sipiczki, M. (1998). Coordination of Initiation of Nuclear Division and Initiation of Cell Division in Schizosaccharomyces pombe: Genetic Interactions of Mutations. J. Bacteriol. 180: 892-900 [Abstract] [Full Text]
Bezanilla, M., Forsburg, S. L., Pollard, T. D. (1997). Identification of a Second Myosin-II in Schizosaccharomyces pombe:. Myp2p Is Conditionally Required for Cytokinesis. Mol. Biol. Cell 8: 2693-2705 [Abstract] [Full Text]
Gould, K. L., Simanis, V. (1997). The control of septum formation in fission�yeast. Genes Dev. 11: 2939-2951 [Full Text]
Spencer, S., Dowbenko, D., Cheng, J., Li, W., Brush, J., Utzig, S., Simanis, V., Lasky, L. A. (1997). PSTPIP: A Tyrosine Phosphorylated Cleavage Furrow-associated Protein that Is a Substrate for a PEST Tyrosine Phosphatase. JCB 138: 845-860 [Abstract] [Full Text]
Kitayama, C., Sugimoto, A., Yamamoto, M. (1997). Type II Myosin Heavy Chain Encoded by the myo2 Gene Composes the Contractile Ring during Cytokinesis in Schizosaccharomyces pombe. JCB 137: 1309-1319 [Abstract] [Full Text]
Schmidt, S, Sohrmann, M, Hofmann, K, Woollard, A, Simanis, V (1997). The Spg1p GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe.. Genes Dev. 11: 1519-1534 [Abstract]
Chang, F., Drubin, D., Nurse, P. (1997). cdc12p, a Protein Required for Cytokinesis in Fission Yeast, Is a Component of the Cell Division Ring and Interacts with Profilin. JCB 137: 169-182 [Abstract] [Full Text]
Sohrmann, M, Fankhauser, C, Brodbeck, C, Simanis, V (1996). The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast.. Genes Dev. 10: 2707-2719 [Abstract]
Chang, F, Woollard, A, Nurse, P (1996). Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring. J. Cell Sci. 109: 131-142 [Abstract]
Desautels, M., Den Haese, J. P., Slupsky, C. M., McIntosh, L. P., Hemmingsen, S. M. (2001). Cdc4p, a Contractile Ring Protein Essential for Cytokinesis in Schizosaccharomyces pombe, Interacts with a Phosphatidylinositol 4-Kinase. J. Biol. Chem. 276: 5932-5942 [Abstract] [Full Text]
Slupsky, C. M., Desautels, M., Huebert, T., Zhao, R., Hemmingsen, S. M., McIntosh, L. P. (2001). Structure of Cdc4p, a Contractile Ring Protein Essential for Cytokinesis in Schizosaccharomyces pombe. J. Biol. Chem. 276: 5943-5951 [Abstract] [Full Text]