Early events in DNA replication require cyclin E and are blocked by p21CIP1

doi:10.1083/jcb.130.4.755

This Article

	Full Text (PDF, 2115K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Jackson, P. K.
	Articles by Kirschner, M. W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Jackson, P. K.
	Articles by Kirschner, M. W.


Social Bookmarking

	What's this?

ARTICLES

Early events in DNA replication require cyclin E and are blocked by p21CIP1

PK Jackson, S Chevalier, M Philippe and MW Kirschner
Harvard Medical School, Department of Cell Biology, Boston, Massachusetts 02115, USA.

Using immunodepletion of cyclin E and the inhibitor protein p21WAF/CIP1, we demonstrate that the cyclin E protein, in association with Cdk2, is required for the elongation phase of replication on single-stranded substrates. Although cyclin E/Cdk2 is likely to be the major target by which p21 inhibits the initiation of sperm DNA replication, p21 can inhibit single-stranded replication through a mechanism dependent on PCNA. While the cyclin E/Cdk2 complex appears to have a role in the initiation of DNA replication, another Cdk kinase, possibly cyclin A/Cdk, may be involved in a later step controlling the switch from initiation to elongation. The provision of a large maternal pool of cyclin E protein shows that regulators of replication are constitutively present, which explains the lack of a protein synthesis requirement for replication in the early embryonic cell cycle.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Lee, H. O., Davidson, J. M., Duronio, R. J. (2009). Endoreplication: polyploidy with purpose. Genes Dev. 23: 2461-2477 [Abstract] [Full Text]
Plesca, D., Mazumder, S., Gama, V., Matsuyama, S., Almasan, A. (2008). A C-terminal Fragment of Cyclin E, Generated by Caspase-mediated Cleavage, Is Degraded in the Absence of a Recognizable Phosphodegron. J. Biol. Chem. 283: 30796-30803 [Abstract] [Full Text]
Labit, H., Perewoska, I., Germe, T., Hyrien, O., Marheineke, K. (2008). DNA replication timing is deterministic at the level of chromosomal domains but stochastic at the level of replicons in Xenopus egg extracts. Nucleic Acids Res 36: 5623-5634 [Abstract] [Full Text]
Mazumder, S., Plesca, D., Kinter, M., Almasan, A. (2007). Interaction of a Cyclin E Fragment with Ku70 Regulates Bax-Mediated Apoptosis. Mol. Cell. Biol. 27: 3511-3520 [Abstract] [Full Text]
Chuang, L.-C., Yew, P. R. (2005). Proliferating Cell Nuclear Antigen Recruits Cyclin-dependent Kinase Inhibitor Xic1 to DNA and Couples Its Proteolysis to DNA Polymerase Switching. J. Biol. Chem. 280: 35299-35309 [Abstract] [Full Text]
Chang, W. Y., Bryce, D. M., D'Souza, S. J. A., Dagnino, L. (2004). The DP-1 Transcription Factor Is Required for Keratinocyte Growth and Epidermal Stratification. J. Biol. Chem. 279: 51343-51353 [Abstract] [Full Text]
Hansen, D. V., Loktev, A. V., Ban, K. H., Jackson, P. K. (2004). Plk1 Regulates Activation of the Anaphase Promoting Complex by Phosphorylating and Triggering SCF{beta}TrCP-dependent Destruction of the APC Inhibitor Emi1. Mol. Biol. Cell 15: 5623-5634 [Abstract] [Full Text]
Richard-Parpaillon, L., Cosgrove, R. A., Devine, C., Vernon, A. E., Philpott, A. (2004). G1/S phase cyclin-dependent kinase overexpression perturbs early development and delays tissue-specific differentiation in Xenopus. Development 131: 2577-2586 [Abstract] [Full Text]
Lindahl, T., Landberg, G., Ahlgren, J., Nordgren, H., Norberg, T., Klaar, S., Holmberg, L., Bergh, J. (2004). Overexpression of cyclin E protein is associated with specific mutation types in the p53 gene and poor survival in human breast cancer. Carcinogenesis 25: 375-380 [Abstract] [Full Text]
Kudoh, A., Daikoku, T., Sugaya, Y., Isomura, H., Fujita, M., Kiyono, T., Nishiyama, Y., Tsurumi, T. (2004). Inhibition of S-Phase Cyclin-Dependent Kinase Activity Blocks Expression of Epstein-Barr Virus Immediate-Early and Early Genes, Preventing Viral Lytic Replication. J. Virol. 78: 104-115 [Abstract] [Full Text]
Prokhorova, T. A., Mowrer, K., Gilbert, C. H., Walter, J. C. (2003). DNA replication of mitotic chromatin in Xenopus egg extracts. Proc. Natl. Acad. Sci. USA 100: 13241-13246 [Abstract] [Full Text]
Moore, J. D., Kirk, J. A., Hunt, T. (2003). Unmasking the S-Phase-Promoting Potential of Cyclin B1. Science 300: 987-990 [Abstract] [Full Text]
Moore, J. D., Kornbluth, S., Hunt, T. (2002). Identification of the Nuclear Localization Signal in Xenopus Cyclin E and Analysis of Its Role in Replication and Mitosis. Mol. Biol. Cell 13: 4388-4400 [Abstract] [Full Text]
Chou, D. M., Petersen, P., Walter, J. C., Walter, G. (2002). Protein Phosphatase 2A Regulates Binding of Cdc45 to the Prereplication Complex. J. Biol. Chem. 277: 40520-40527 [Abstract] [Full Text]
Malcles, M.-H., Cueille, N., Mechali, F., Coux, O., Bonne-Andrea, C. (2002). Regulation of Bovine Papillomavirus Replicative Helicase E1 by the Ubiquitin-Proteasome Pathway. J. Virol. 76: 11350-11358 [Abstract] [Full Text]
Geisen, C., Moroy, T. (2002). The Oncogenic Activity of Cyclin E Is Not Confined to Cdk2 Activation Alone but Relies on Several Other, Distinct Functions of the Protein. J. Biol. Chem. 277: 39909-39918 [Abstract] [Full Text]
Lupardus, P. J., Byun, T., Yee, M.-c., Hekmat-Nejad, M., Cimprich, K. A. (2002). A requirement for replication in activation of the ATR-dependent DNA damage checkpoint. Genes Dev. 16: 2327-2332 [Abstract] [Full Text]
Mazumder, S., Gong, B., Chen, Q., Drazba, J. A., Buchsbaum, J. C., Almasan, A. (2002). Proteolytic Cleavage of Cyclin E Leads to Inactivation of Associated Kinase Activity and Amplification of Apoptosis in Hematopoietic Cells. Mol. Cell. Biol. 22: 2398-2409 [Abstract] [Full Text]
Jackman, M., Kubota, Y., den Elzen, N., Hagting, A., Pines, J. (2002). Cyclin A- and Cyclin E-Cdk Complexes Shuttle between the Nucleus and the Cytoplasm. Mol. Biol. Cell 13: 1030-1045 [Abstract] [Full Text]
Chien, W.-M., Noya, F., Benedict-Hamilton, H. M., Broker, T. R., Chow, L. T. (2002). Alternative Fates of Keratinocytes Transduced by Human Papillomavirus Type 18 E7 during Squamous Differentiation. J. Virol. 76: 2964-2972 [Abstract] [Full Text]
Schnackenberg, B. J., Marzluff, W. F. (2002). Novel localization and possible functions of cyclin E in early sea urchin development. J. Cell Sci. 115: 113-121 [Abstract] [Full Text]
Kim, T.-Y., Kaelin, W. G. Jr. (2001). Differential Control of Transcription by DNA-bound Cyclins. Mol. Biol. Cell 12: 2207-2217 [Abstract] [Full Text]
Hinchcliffe, E. H., Sluder, G. (2001). "It Takes Two to Tango": understanding how centrosome duplication is regulated throughout the cell cycle. Genes Dev. 15: 1167-1181 [Full Text]
Bashir, T., Rommelaere, J., Cziepluch, C. (2001). In Vivo Accumulation of Cyclin A and Cellular Replication Factors in Autonomous Parvovirus Minute Virus of Mice-Associated Replication Bodies. J. Virol. 75: 4394-4398 [Abstract] [Full Text]
Ekholm, S. V., Zickert, P., Reed, S. I., Zetterberg, A. (2001). Accumulation of Cyclin E Is Not a Prerequisite for Passage through the Restriction Point. Mol. Cell. Biol. 21: 3256-3265 [Abstract] [Full Text]
Furstenthal, L., Kaiser, B. K., Swanson, C., Jackson, P. K. (2001). Cyclin E Uses Cdc6 as a Chromatin-Associated Receptor Required for DNA Replication. JCB 152: 1267-1278 [Abstract] [Full Text]
Sørensen, C. S., Lukas, C., Kramer, E. R., Peters, J.-M., Bartek, J., Lukas, J. (2000). Nonperiodic Activity of the Human Anaphase-Promoting Complex-Cdh1 Ubiquitin Ligase Results in Continuous DNA Synthesis Uncoupled from Mitosis. Mol. Cell. Biol. 20: 7613-7623 [Abstract] [Full Text]
Liao, S., Graham, J., Yan, H. (2000). The function of Xenopus Bloom's syndrome protein homolog (xBLM) in DNA replication. Genes Dev. 14: 2570-2575 [Abstract] [Full Text]
Ewen, M. E. (2000). Where the cell cycle and histones meet. Genes Dev. 14: 2265-2270 [Full Text]
Zhao, J., Kennedy, B. K., Lawrence, B. D., Barbie, D. A., Matera, A. G., Fletcher, J. A., Harlow, E. (2000). NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription. Genes Dev. 14: 2283-2297 [Abstract] [Full Text]
Kappas, N. C., Savage, P., Chen, K. C., Walls, A. T., Sible, J. C. (2000). Dissection of the XChk1 Signaling Pathway in Xenopus laevis Embryos. Mol. Biol. Cell 11: 3101-3108 [Abstract] [Full Text]
Swanson, C., Ross, J., Jackson, P. K. (2000). Nuclear accumulation of cyclin E/Cdk2 triggers a concentration-dependent switch for the destruction of p27Xic1. Proc. Natl. Acad. Sci. USA 97: 7796-7801 [Abstract] [Full Text]
Santoni-Rugiu, E., Falck, J., Mailand, N., Bartek, J., Lukas, J. (2000). Involvement of Myc Activity in a G1/S-Promoting Mechanism Parallel to the pRb/E2F Pathway. Mol. Cell. Biol. 20: 3497-3509 [Abstract] [Full Text]
Lane, M. E., Elend, M., Heidmann, D., Herr, A., Marzodko, S., Herzig, A., Lehner, C. F. (2000). A Screen for Modifiers of Cyclin E Function in Drosophila melanogaster Identifies Cdk2 Mutations, Revealing the Insignificance of Putative Phosphorylation Sites in Cdk2. Genetics 155: 233-244 [Abstract] [Full Text]
Lin, B. Y., Ma, T., Liu, J.-S., Kuo, S.-R., Jin, G., Broker, T. R., Harper, J. W., Chow, L. T. (2000). HeLa Cells Are Phenotypically Limiting in Cyclin E/CDK2 for Efficient Human Papillomavirus DNA Replication. J. Biol. Chem. 275: 6167-6174 [Abstract] [Full Text]
Romanowski, P., Marr, J., Madine, M. A., Rowles, A., Blow, J. J., Gautier, J., Laskey, R. A. (2000). Interaction of Xenopus Cdc2{middle dot}Cyclin A1 with the Origin Recognition Complex. J. Biol. Chem. 275: 4239-4243 [Abstract] [Full Text]
Sun, W, Hola, M, Pedley, K, Tada, S, Blow, J., Todorov, I., Kearsey, S., Brooks, R. (2000). The replication capacity of intact mammalian nuclei in Xenopus egg extracts declines with quiescence, but the residual DNA synthesis is independent of Xenopus MCM proteins. J. Cell Sci. 113: 683-695 [Abstract]
Lu, Z. H., Xu, H., Leno, G. H. (1999). DNA Replication in Quiescent Cell Nuclei: Regulation by the Nuclear Envelope and Chromatin Structure. Mol. Biol. Cell 10: 4091-4106 [Abstract] [Full Text]
Singer, J. D., Gurian-West, M., Clurman, B., Roberts, J. M. (1999). Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev. 13: 2375-2387 [Abstract] [Full Text]
Freed, E., Lacey, K. R., Huie, P., Lyapina, S. A., Deshaies, R. J., Stearns, T., Jackson, P. K. (1999). Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle. Genes Dev. 13: 2242-2257 [Abstract] [Full Text]
Sherr, C. J., Roberts, J. M. (1999). CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Dev. 13: 1501-1512 [Full Text]
Lacey, K. R., Jackson, P. K., Stearns, T. (1999). Cyclin-dependent kinase control of centrosome duplication. Proc. Natl. Acad. Sci. USA 96: 2817-2822 [Abstract] [Full Text]
Jian, Y., Van Tine, B. A., Chien, W.-M., Shaw, G. M., Broker, T. R., Chow, L. T. (1999). Concordant Induction of Cyclin E and p21cip1 in Differentiated Keratinocytes by the Human Papillomavirus E7 Protein Inhibits Cellular and Viral DNA Synthesis. Cell Growth Differ. 10: 101-111 [Abstract] [Full Text]
Rowles, A, Tada, S, Blow, J. (1999). Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins. J. Cell Sci. 112: 2011-2018 [Abstract]
Carpenter, P. B., Dunphy, W. G. (1998). Identification of a Novel 81-kDa Component of the Xenopus Origin Recognition Complex. J. Biol. Chem. 273: 24891-24897 [Abstract] [Full Text]
Cueille, N., Nougarede, R., Mechali, F., Philippe, M., Bonne-Andrea, C. (1998). Functional Interaction between the Bovine Papillomavirus Virus Type 1�Replicative Helicase E1 and Cyclin E-Cdk2. J. Virol. 72: 7255-7262 [Abstract] [Full Text]
Guo, K., Andres, V., Walsh, K. (1998). Nitric Oxide�Induced Downregulation of Cdk2 Activity and Cyclin A Gene Transcription in Vascular Smooth Muscle Cells. Circulation 97: 2066-2072 [Abstract] [Full Text]
Sterner, J. M., Dew-Knight, S., Musahl, C., Kornbluth, S., Horowitz, J. M. (1998). Negative Regulation of DNA Replication by the Retinoblastoma Protein Is Mediated by Its Association with MCM7. Mol. Cell. Biol. 18: 2748-2757 [Abstract] [Full Text]
Lu, Z. H., Sittman, D. B., Romanowski, P., Leno, G. H. (1998). Histone H1 Reduces the Frequency of Initiation in Xenopus Egg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin. Mol. Biol. Cell 9: 1163-1176 [Abstract] [Full Text]
Su, T. T., O'Farrell, P. H. (1998). Chromosome Association of Minichromosome Maintenance Proteins in Drosophila Endoreplication Cycles. JCB 140: 451-460 [Abstract] [Full Text]
Harvat, B., Wang, A, Seth, P, Jetten, A. (1998). Up-regulation of p27Kip1, p21WAF1/Cip1 and p16Ink4a is associated with, but not sufficient for, induction of squamous differentiation. J. Cell Sci. 111: 1185-1196 [Abstract]
Duronio, R. J., Bonnette, P. C., O'Farrell, P. H. (1998). Mutations of the Drosophila dDP, dE2F, and cyclin E Genes Reveal Distinct Roles for the E2F-DP Transcription Factor and Cyclin E during the G1-S Transition. Mol. Cell. Biol. 18: 141-151 [Abstract] [Full Text]
Murakami, M., Vande Woude, G. (1998). Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos. Development 125: 237-248 [Abstract]
Lopez-Girona, A., Mondesert, O., Leatherwood, J., Russell, P. (1998). Negative Regulation of Cdc18 DNA Replication Protein by Cdc2. Mol. Biol. Cell 9: 63-73 [Abstract] [Full Text]
Su, T. T., O'Farrell, P. H. (1997). Chromosome Association of Minichromosome Maintenance Proteins in Drosophila Mitotic Cycles. JCB 139: 13-21 [Abstract] [Full Text]
Yew, P. R., Kirschner, M. W. (1997). Proteolysis and DNA Replication: The CDC34 Requirement in the Xenopus Egg Cell Cycle. Science 277: 1672-1676 [Abstract] [Full Text]
Hua, X. H., Yan, H., Newport, J. (1997). A Role for Cdk2 Kinase in Negatively Regulating DNA Replication during S Phase of the Cell Cycle. JCB 137: 183-192 [Abstract] [Full Text]
Mahbubani, H. M., Chong, J. P.J., Chevalier, S., Thommes, P., Blow, J. J. (1997). Cell Cycle Regulation of the Replication Licensing System: Involvement of a Cdk-dependent Inhibitor. JCB 136: 125-135 [Abstract] [Full Text]
Wolf, D. A., Wu, D., McKeon, F. (1996). Disruption of Re-replication Control by Overexpression of Human ORC1 in Fission Yeast. J. Biol. Chem. 271: 32503-32506 [Abstract] [Full Text]
Fotedar, A., Cannella, D., Fitzgerald, P., Rousselle, T., Gupta, S., Doree, M., Fotedar, R. (1996). Role for Cyclin A-dependent Kinase in DNA Replication in Human S Phase Cell Extracts. J. Biol. Chem. 271: 31627-31637 [Abstract] [Full Text]
Duronio, R J, Brook, A, Dyson, N, O'Farrell, P H (1996). E2F-induced S phase requires cyclin E.. Genes Dev. 10: 2505-2513 [Abstract]
Chevalier, S, Couturier, A, Chartrain, I, Le Guellec, R, Beckhelling, C, Le Guellec, K, Philippe, M, Ford, C. (1996). Xenopus cyclin E, a nuclear phosphoprotein, accumulates when oocytes gain the ability to initiate DNA replication. J. Cell Sci. 109: 1173-1184 [Abstract]
Strausfeld, U., Howell, M, Descombes, P, Chevalier, S, Rempel, R., Adamczewski, J, Maller, J., Hunt, T, Blow, J. (1996). Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts. J. Cell Sci. 109: 1555-1563 [Abstract]
Jones, C, Smythe, C (1996). Activation of the Xenopus cyclin degradation machinery by full-length cyclin A. J. Cell Sci. 109: 1071-1079 [Abstract]
Moos, J, Kopf, G., Schultz, R. (1996). Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities. J. Cell Sci. 109: 739-748 [Abstract]
Walter, J. C. (2000). Evidence for Sequential Action of cdc7 and cdk2 Protein Kinases during Initiation of DNA Replication in Xenopus Egg Extracts. J. Biol. Chem. 275: 39773-39778 [Abstract] [Full Text]
Bashir, T., Horlein, R., Rommelaere, J., Willwand, K. (2000). Cyclin A activates the DNA polymerase delta -dependent elongation machinery in vitro: A parvovirus DNA replication model. Proc. Natl. Acad. Sci. USA 97: 5522-5527 [Abstract] [Full Text]