Rigidity of microtubules is increased by stabilizing agents

doi:10.1083/jcb.130.4.909

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Rigidity of microtubules is increased by stabilizing agents

B Mickey and J Howard
Department of Physiology and Biophysics, University of Washington, Seattle 98195-7290, USA.

Microtubules are rigid polymers that contribute to the static mechanical properties of cells. Because microtubules are dynamic structures whose polymerization is regulated during changes in cell shape, we have asked whether the mechanical properties of microtubules might also be modulated. We measured the flexural rigidity, or bending stiffness, of individual microtubules under a number of different conditions that affect the stability of microtubules against depolymerization. The flexural rigidity of microtubules polymerized with the slowly hydrolyzable nucleotide analogue guanylyl-(alpha, beta)- methylene-diphosphonate was 62 +/- 9 x 10(-24) Nm2 (weighted mean +/- SEM); that of microtubules stabilized with tau protein was 34 +/- 3 x 10(-24) Nm2; and that of microtubules stabilized with the antimitotic drug taxol was 32 +/- 2 x 10(-24) Nm2. For comparison, microtubules that were capped to prevent depolymerization, but were not otherwise stabilized, had a flexural rigidity of 26 +/- 2 x 10(-24) Nm2. Decreasing the temperature from 37 degrees C to approximately 25 degrees C, a condition that makes microtubules less stable, decreased the stiffness of taxol-stabilized microtubules by one-third. We thus find that the more stable a microtubule, the higher its flexural rigidity. This raises the possibility that microtubule rigidity may be regulated in vivo. In addition, the high rigidity of an unstabilized, GDP-containing microtubule suggests that a large amount of energy could be stored as mechanical strain energy in the protein lattice for subsequent force generation during microtubule depolymerization.
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Cassimeris, L., Gard, D., Tran, P. T., Erickson, H. P. (2002). XMAP215 is a long thin molecule that does not increase microtubule stiffness. J. Cell Sci. 114: 3025-3033 [Abstract] [Full Text]
Tran, P.T., Marsh, L., Doye, V., Inoue, S., Chang, F. (2001). A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing. JCB 153: 397-412 [Abstract] [Full Text]
Hunter, A., Wordeman, L (2000). How motor proteins influence microtubule polymerization dynamics. J. Cell Sci. 113: 4379-4389 [Abstract]
Theodoropoulos, P. A., Polioudaki, H., Kostaki, O., Derdas, S. P., Georgoulias, V., Dargemont, C., Georgatos, S. D. (1999). Taxol Affects Nuclear Lamina and Pore Complex Organization and Inhibits Import of Karyophilic Proteins into the Cell Nucleus. Cancer Res. 59: 4625-4633 [Abstract] [Full Text]
Hegner, M., Smith, S. B., Bustamante, C. (1999). Polymerization and mechanical properties of single RecA-DNA filaments. Proc. Natl. Acad. Sci. USA 96: 10109-10114 [Abstract] [Full Text]
Coy, D. L., Wagenbach, M., Howard, J. (1999). Kinesin Takes One 8-nm Step for Each ATP That It Hydrolyzes. J. Biol. Chem. 274: 3667-3671 [Abstract] [Full Text]
Diaz, J. F., Valpuesta, J. M., Chacon, P., Diakun, G., Andreu, J. M. (1998). Changes in Microtubule Protofilament Number Induced by Taxol Binding to an Easily Accessible Site. INTERNAL MICROTUBULE DYNAMICS. J. Biol. Chem. 273: 33803-33810 [Abstract] [Full Text]
Hunt, A. J., McIntosh, J. R. (1998). The Dynamic Behavior of Individual Microtubules Associated with Chromosomes In Vitro. Mol. Biol. Cell 9: 2857-2871 [Abstract] [Full Text]
Muller-Reichert, T., Chretien, D., Severin, F., Hyman, A. A. (1998). Structural changes at microtubule ends accompanying GTP hydrolysis: Information from a slowly hydrolyzable analogue of GTP, guanylyl (alpha ,beta )methylenediphosphonate. Proc. Natl. Acad. Sci. USA 95: 3661-3666 [Abstract] [Full Text]
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Dogterom, M., Yurke, B. (1997). Measurement of the Force-Velocity Relation for Growing Microtubules. Science 278: 856-860 [Abstract] [Full Text]
Felgner, H., Frank, R., Biernat, J., Mandelkow, E.-M., Mandelkow, E., Ludin, B., Matus, A., Schliwa, M. (1997). Domains of Neuronal Microtubule-associated Proteins and Flexural Rigidity of Microtubules. JCB 138: 1067-1075 [Abstract] [Full Text]
Tran, P.T., Walker, R.A., Salmon, E.D. (1997). A Metastable Intermediate State of Microtubule Dynamic Instability That Differs Significantly between Plus and Minus Ends. JCB 138: 105-117 [Abstract] [Full Text]
Holy, T. E., Dogterom, M., Yurke, B., Leibler, S. (1997). Assembly and positioning of microtubule asters in microfabricated�chambers. Proc. Natl. Acad. Sci. USA 94: 6228-6231 [Abstract] [Full Text]
Challacombe, J. F., Snow, D. M., Letourneau, P. C. (1997). Dynamic Microtubule Ends Are Required for Growth Cone Turning to Avoid an Inhibitory Guidance Cue. J. Neurosci. 17: 3085-3095 [Abstract] [Full Text]
Panda, D., Jordan, M. A., Chu, K. C., Wilson, L. (1996). Differential Effects of Vinblastine on Polymerization and Dynamics at Opposite Microtubule Ends. J. Biol. Chem. 271: 29807-29812 [Abstract] [Full Text]
Fishkind, D., Silverman, J., Wang, Y. (1996). Function of spindle microtubules in directing cortical movement and actin filament organization in dividing cultured cells. J. Cell Sci. 109: 2041-2051 [Abstract]
Felgner, H, Frank, R, Schliwa, M (1996). Flexural rigidity of microtubules measured with the use of optical tweezers. J. Cell Sci. 109: 509-516 [Abstract]