Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide- specific monoclonal antibodies

doi:10.1083/jcb.130.5.1219

This Article

	Full Text (PDF, 7403K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ninomiya, Y.
	Articles by Sado, Y.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ninomiya, Y.
	Articles by Sado, Y.


Social Bookmarking

	What's this?

ARTICLES

Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide- specific monoclonal antibodies

Y Ninomiya, M Kagawa, K Iyama, I Naito, Y Kishiro, JM Seyer, M Sugimoto, T Oohashi and Y Sado
Department of Molecular Biology, Okayama University Medical School, Japan.

Genes for the human alpha 5(IV) and alpha 6(IV) collagen chains have a unique arrangement in that they are colocalized on chromosome Xq22 in a head-to-head fashion and appear to share a common bidirectional promoter. In addition we reported a novel observation that the COL4A6 gene is transcribed from two alternative promoters in a tissue-specific manner (Sugimoto, M., T. Oohashi, and Y. Ninomiya. 1994. Proc. Natl. Acad. Sci. USA. 91:11679-11683). To know whether the translation products of both genes are colocalized in various tissues, we raised alpha 5(IV) and alpha 6(IV) chain-specific rat monoclonal antibodies against synthetic peptides reflecting sequences near the carboxy terminus of each noncollagenous (NC)1 domain. By Western blotting alpha 6(IV) chain-specific antibody recognized 27-kD monomers and associated dimers of the human type IV collagen NC1 domain, which is the first demonstration of the presence in tissues of the alpha 6(IV) polypeptide as predicted from its cDNA sequence. Immunofluorescence studies using anti-alpha 6(IV) antibody demonstrated that in human adult kidney the alpha 6(IV) chain was never detected in the glomerular basement membrane, whereas the basement membranes of the Bowman's capsules and distal tubules were positive. The staining pattern of the glomerular basement membrane was quite different from that obtained with the anti- alpha 5(IV) peptide antibody. The alpha 5(IV) and alpha 6(IV) chains were colocalized in the basement membrane in the skin, smooth muscle cells, and adipocytes; however, little if any reaction was seen in basement membranes of cardiac muscles and hepatic sinusoidal endothelial cells. Thus, both genes are expressed in a tissue-specific manner, perhaps due to the unique function of the bidirectional promoter for both genes, which is presumably different from that for COL4A1 and COL4A2.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Abrahamson, D. R., Hudson, B. G., Stroganova, L., Borza, D.-B., St. John, P. L. (2009). Cellular Origins of Type IV Collagen Networks in Developing Glomeruli. J. Am. Soc. Nephrol. 20: 1471-1479 [Abstract] [Full Text]
Tveita, A., Ninomiya, Y, Sado, Y, Rekvig, O., Zykova, S. (2009). Development of lupus nephritis is associated with qualitative changes in the glomerular collagen IV matrix composition. Lupus 18: 355-360 [Abstract]
Shim, J., Moulson, C. L., Newberry, E. P., Lin, M.-H., Xie, Y., Kennedy, S. M., Miner, J. H., Davidson, N. O. (2009). Fatty acid transport protein 4 is dispensable for intestinal lipid absorption in mice. J. Lipid Res. 50: 491-500 [Abstract] [Full Text]
Vanacore, R. M., Ham, A.-J. L., Cartailler, J.-P., Sundaramoorthy, M., Todd, P., Pedchenko, V., Sado, Y., Borza, D.-B., Hudson, B. G. (2008). A Role for Collagen IV Cross-links in Conferring Immune Privilege to the Goodpasture Autoantigen: STRUCTURAL BASIS FOR THE CRYPTICITY OF B CELL EPITOPES. J. Biol. Chem. 283: 22737-22748 [Abstract] [Full Text]
Thorner, P. S., Ho, M., Eremina, V., Sado, Y., Quaggin, S. (2008). Podocytes Contribute to the Formation of Glomerular Crescents. J. Am. Soc. Nephrol. 19: 495-502 [Abstract] [Full Text]
Kabosova, A., Azar, D. T., Bannikov, G. A., Campbell, K. P., Durbeej, M., Ghohestani, R. F., Jones, J. C. R., Kenney, M. C., Koch, M., Ninomiya, Y., Patton, B. L., Paulsson, M., Sado, Y., Sage, E. H., Sasaki, T., Sorokin, L. M., Steiner-Champliaud, M.-F., Sun, T.-T., SundarRaj, N., Timpl, R., Virtanen, I., Ljubimov, A. V. (2007). Compositional Differences between Infant and Adult Human Corneal Basement Membranes. IOVS 48: 4989-4999 [Abstract] [Full Text]
Guerriero, E., Chen, J., Sado, Y., Mohan, R. R., Wilson, S. E., Funderburgh, J. L., SundarRaj, N. (2007). Loss of Alpha3(IV) Collagen Expression Associated with Corneal Keratocyte Activation. IOVS 48: 627-635 [Abstract] [Full Text]
Chen, L., Hellmark, T., Pedchenko, V., Hudson, B. G., Pusey, C. D., Fox, J. W., Bolton, W. K. (2006). A Nephritogenic Peptide Induces Intermolecular Epitope Spreading on Collagen IV in Experimental Autoimmune Glomerulonephritis. J. Am. Soc. Nephrol. 17: 3076-3081 [Abstract] [Full Text]
Harvey, S. J., Perry, J., Zheng, K., Chen, D., Sado, Y., Jefferson, B., Ninomiya, Y., Jacobs, R., Hudson, B. G., Thorner, P. S. (2006). Sequential Expression of Type IV Collagen Networks: Testis as a Model and Relevance to Spermatogenesis. Am. J. Pathol. 168: 1587-1597 [Abstract] [Full Text]
El-Aouni, C., Herbach, N., Blattner, S. M., Henger, A., Rastaldi, M. P., Jarad, G., Miner, J. H., Moeller, M. J., St-Arnaud, R., Dedhar, S., Holzman, L. B., Wanke, R., Kretzler, M. (2006). Podocyte-Specific Deletion of Integrin-Linked Kinase Results in Severe Glomerular Basement Membrane Alterations and Progressive Glomerulosclerosis. J. Am. Soc. Nephrol. 17: 1334-1344 [Abstract] [Full Text]
Ikeda, K., Iyama, K.-i., Ishikawa, N., Egami, H., Nakao, M., Sado, Y., Ninomiya, Y., Baba, H. (2006). Loss of Expression of Type IV Collagen {alpha}5 and {alpha}6 Chains in Colorectal Cancer Associated with the Hypermethylation of Their Promoter Region. Am. J. Pathol. 168: 856-865 [Abstract] [Full Text]
MacDonald, B. A., Sund, M., Grant, M. A., Pfaff, K. L., Holthaus, K., Zon, L. I., Kalluri, R. (2006). Zebrafish to humans: evolution of the {alpha}3-chain of type IV collagen and emergence of the autoimmune epitopes associated with Goodpasture syndrome. Blood 107: 1908-1915 [Abstract] [Full Text]
Perry, J., Tam, S., Zheng, K., Sado, Y., Dobson, H., Jefferson, B., Jacobs, R., Thorner, P. S. (2006). Type IV Collagen Induces Podocytic Features in Bone Marrow Stromal Stem Cells In Vitro. J. Am. Soc. Nephrol. 17: 66-76 [Abstract] [Full Text]
Wu, J., Arends, J., Borillo, J., Zhou, C., Merszei, J., McMahon, J., Lou, Y.-H. (2004). A Self T Cell Epitope Induces Autoantibody Response: Mechanism for Production of Antibodies to Diverse Glomerular Basement Membrane Antigens. J. Immunol. 172: 4567-4574 [Abstract] [Full Text]
Poschl, E., Schlotzer-Schrehardt, U., Brachvogel, B., Saito, K., Ninomiya, Y., Mayer, U. (2004). Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development. Development 131: 1619-1628 [Abstract] [Full Text]
Nomoto, H., Yonezawa, T., Itoh, K., Ono, K., Yamamoto, K., Oohashi, T., Shiraga, F., Ohtsuki, H., Ninomiya, Y. (2003). Molecular Cloning of a Novel Transmembrane Protein MOLT Expressed by Mature Oligodendrocytes. J Biochem 134: 231-238 [Abstract] [Full Text]
Hudson, B. G., Tryggvason, K., Sundaramoorthy, M., Neilson, E. G. (2003). Alport's Syndrome, Goodpasture's Syndrome, and Type IV Collagen. NEJM 348: 2543-2556 [Full Text]
Kikkawa, Y., Virtanen, I., Miner, J. H. (2003). Mesangial cells organize the glomerular capillaries by adhering to the G domain of laminin {alpha}5 in the glomerular basement membrane. JCB 161: 187-196 [Abstract] [Full Text]
Chen, D., Jefferson, B., Harvey, S. J., Zheng, K., Gartley, C. J., Jacobs, R. M., Thorner, P. S. (2003). Cyclosporine A Slows the Progressive Renal Disease of Alport Syndrome (X-Linked Hereditary Nephritis): Results from a Canine Model. J. Am. Soc. Nephrol. 14: 690-698 [Abstract] [Full Text]
Harvey, S. J., Zheng, K., Jefferson, B., Moak, P., Sado, Y., Naito, I., Ninomiya, Y., Jacobs, R., Thorner, P. S. (2003). Transfer of the {alpha}5(IV) Collagen Chain Gene to Smooth Muscle Restores in Vivo Expression of the {alpha}6(IV) Collagen Chain in a Canine Model of Alport Syndrome. Am. J. Pathol. 162: 873-885 [Abstract] [Full Text]
Chen, L, Miyamura, N, Ninomiya, Y, Handa, J T (2003). Distribution of the collagen IV isoforms in human Bruch's membrane. Br J Ophthalmol 87: 212-215 [Abstract] [Full Text]
Borza, D.-B., Bondar, O., Todd, P., Sundaramoorthy, M., Sado, Y., Ninomiya, Y., Hudson, B. G. (2002). Quaternary Organization of the Goodpasture Autoantigen, the alpha 3(IV) Collagen Chain. SEQUESTRATION OF TWO CRYPTIC AUTOEPITOPES BY INTRAPROTOMER INTERACTIONS WITH THE alpha 4 AND alpha 5 NC1 DOMAINS. J. Biol. Chem. 277: 40075-40083 [Abstract] [Full Text]
Mothes, H., Heidet, L., Arrondel, C., Richter, K. K., Thiele, M., Patzer, L., Sado, Y., Gubler, M.-C., Antignac, C., Scheele, J. (2002). Alport syndrome associated with diffuse leiomyomatosis: COL4A5-COL4A6 deletion associated with a mild form of Alport nephropathy. Nephrol Dial Transplant 17: 70-74 [Abstract] [Full Text]
Harvey, S. J., Mount, R., Sado, Y., Naito, I., Ninomiya, Y., Harrison, R., Jefferson, B., Jacobs, R., Thorner, P. S. (2001). The Inner Ear of Dogs with X-Linked Nephritis Provides Clues to the Pathogenesis of Hearing Loss in X-Linked Alport Syndrome. Am. J. Pathol. 159: 1097-1104 [Abstract] [Full Text]
Wu, J., Hicks, J., Ou, C.-n., Singleton, D., Borillo, J., Lou, Y.-H. (2001). Glomerulonephritis Induced by Recombinant Collagen IV{alpha}3 Chain Noncollagen Domain 1 Is Not Associated with Glomerular Basement Membrane Antibody: A Potential T Cell-Mediated Mechanism. J. Immunol. 167: 2388-2395 [Abstract] [Full Text]
HEIDET, L., ARRONDEL, C., FORESTIER, L., COHEN-SOLAL, L., MOLLET, G., GUTIERREZ, B., STAVROU, C., GUBLER, M. C., ANTIGNAC, C. (2001). Structure of the Human Type IV Collagen Gene COL4A3 and Mutations in Autosomal Alport Syndrome. J. Am. Soc. Nephrol. 12: 97-106 [Abstract] [Full Text]
Fleischmajer, R, Utani, A, MacDonald, E., Perlish, J., Pan, T., Chu, M., Nomizu, M, Ninomiya, Y, Yamada, Y (2000). Initiation of skin basement membrane formation at the epidermo-dermal interface involves assembly of laminins through binding to cell membrane receptors. J. Cell Sci. 111: 1929-1940 [Abstract]
Heidet, L., Cai, Y., Guicharnaud, L., Antignac, C., Gubler, M.-C. (2000). Glomerular Expression of Type IV Collagen Chains in Normal and X-Linked Alport Syndrome Kidneys. Am. J. Pathol. 156: 1901-1910 [Abstract] [Full Text]
Catusse, C., Polette, M., Coraux, C., Burlet, H., Birembaut, P. (2000). Modified Basement Membrane Composition During Bronchopulmonary Tumor Progression. J. Histochem. Cytochem. 48: 663-670 [Abstract] [Full Text]
Kalluri, R., Cosgrove, D. (2000). Assembly of Type IV Collagen. INSIGHTS FROM alpha 3(IV) COLLAGEN-DEFICIENT MICE. J. Biol. Chem. 275: 12719-12724 [Abstract] [Full Text]
Petitclerc, E., Boutaud, A., Prestayko, A., Xu, J., Sado, Y., Ninomiya, Y., Sarras, M. P. Jr., Hudson, B. G., Brooks, P. C. (2000). New Functions for Non-collagenous Domains of Human Collagen Type IV. NOVEL INTEGRIN LIGANDS INHIBITING ANGIOGENESIS AND TUMOR GROWTH IN VIVO. J. Biol. Chem. 275: 8051-8061 [Abstract] [Full Text]
Miner, J. H. (1999). Alport Syndrome with Diffuse Leiomyomatosis : When and When Not?. Am. J. Pathol. 154: 1633-1635 [Full Text]
Zheng, K., Harvey, S., Sado, Y., Naito, I., Ninomiya, Y., Jacobs, R., Thorner, P. S. (1999). Absence of the {alpha}6(IV) Chain of Collagen Type IV in Alport Syndrome Is Related to a Failure at the Protein Assembly Level and Does Not Result in Diffuse Leiomyomatosis. Am. J. Pathol. 154: 1883-1891 [Abstract] [Full Text]
Tiger, C.-F., Champliaud, M.-F., Pedrosa-Domellof, F., Thornell, L.-E., Ekblom, P., Gullberg, D. (1997). Presence of Laminin alpha 5 Chain and Lack of Laminin alpha 1 Chain during Human Muscle Development and in Muscular Dystrophies. J. Biol. Chem. 272: 28590-28595 [Abstract] [Full Text]
Kahsai, T. Z., Enders, G. C., Gunwar, S., Brunmark, C., Wieslander, J., Kalluri, R., Zhou, J., Noelken, M. E., Hudson, B. G. (1997). Seminiferous Tubule Basement Membrane. COMPOSITION AND ORGANIZATION OF TYPE IV COLLAGEN CHAINS, AND THE LINKAGE OF alpha 3(IV) AND alpha 5(IV) CHAINS. J. Biol. Chem. 272: 17023-17032 [Abstract] [Full Text]
Graham, P. L., Johnson, J. J., Wang, S., Sibley, M. H., Gupta, M. C., Kramer, J. M. (1997). Type IV Collagen Is Detectable in Most, but Not All, Basement Membranes of Caenorhabditis elegans and Assembles on Tissues That Do Not Express It. JCB 137: 1171-1183 [Abstract] [Full Text]
Gupta, M. C., Graham, P. L., Kramer, J. M. (1997). Characterization of {alpha}1(IV) Collagen Mutations in Caenorhabditis elegans and the Effects of {alpha}1 and {alpha}2(IV) Mutations on Type IV Collagen Distribution. JCB 137: 1185-1196 [Abstract] [Full Text]
Cosgrove, D, Meehan, D T, Grunkemeyer, J A, Kornak, J M, Sayers, R, Hunter, W J, Samuelson, G C (1996). Collagen COL4A3 knockout: a mouse model for autosomal Alport syndrome.. Genes Dev. 10: 2981-2992 [Abstract]
Thorner, P. S., Zheng, K., Kalluri, R., Jacobs, R., Hudson, B. G. (1996). Coordinate Gene Expression of the alpha 3, alpha 4, and alpha 5 Chains of Collagen Type IV. EVIDENCE FROM A CANINE MODEL OF X-LINKED NEPHRITIS WITH A COL4A5 GENE MUTATION. J. Biol. Chem. 271: 13821-13828 [Abstract] [Full Text]
Oohashi, T., Ueki, Y., Sugimoto, M., Ninomiya, Y. (1995). Isolation and Structure of the COL4A6 Gene Encoding the Human alpha6(IV) Collagen Chain and Comparison with Other Type IV Collagen Genes. J. Biol. Chem. 270: 26863-26867 [Abstract] [Full Text]
Boutaud, A., Borza, D.-B., Bondar, O., Gunwar, S., Netzer, K.-O., Singh, N., Ninomiya, Y., Sado, Y., Noelken, M. E., Hudson, B. G. (2000). Type IV Collagen of the Glomerular Basement Membrane. EVIDENCE THAT THE CHAIN SPECIFICITY OF NETWORK ASSEMBLY IS ENCODED BY THE NONCOLLAGENOUS NC1 DOMAINS. J. Biol. Chem. 275: 30716-30724 [Abstract] [Full Text]
Segal, Y., Zhuang, L., Rondeau, E., Sraer, J.-D., Zhou, J. (2001). Regulation of the Paired Type IV Collagen Genes COL4A5 and COL4A6. ROLE OF THE PROXIMAL PROMOTER REGION. J. Biol. Chem. 276: 11791-11797 [Abstract] [Full Text]
Borza, D.-B., Bondar, O., Ninomiya, Y., Sado, Y., Naito, I., Todd, P., Hudson, B. G. (2001). The NC1 Domain of Collagen IV Encodes a Novel Network Composed of the alpha 1, alpha 2, alpha 5, and alpha 6 Chains in Smooth Muscle Basement Membranes. J. Biol. Chem. 276: 28532-28540 [Abstract] [Full Text]