Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD1)

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Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD1)

MS Diamond, R Alon, CA Parkos, MT Quinn and TA Springer
Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA.

Previous studies have demonstrated that the leukocyte integrin Mac-1 adheres to several cell surface and soluble ligands including intercellular adhesion molecule-1, fibrinogen, iC3b, and factor X. However, experiments with Mac-1-expressing transfectants, purified Mac- 1, and mAbs to Mac-1 indicate the existence of additional ligands. In this paper, we demonstrate a direct interaction between Mac-1 and heparan sulfate glycans. Heparin affinity resins immunoprecipitate Mac- 1, and neutrophils and transfectant cells that express Mac-1 bind to heparin and heparan sulfate, but not to other sulfated glycosaminoglycans. Inhibition studies with mAbs and chemically modified forms of heparin suggest the I domain as a recognition site on Mac-1 for heparin, and suggest that either N- or O-sulfation is sufficient for heparin to bind efficiently to Mac-1. Under conditions of continuous flow in which heparins and E-selectin are cosubstrates, neutrophils tether to E-selectin and form firm adhesions through a Mac- 1-heparin interaction.
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Hidalgo, A., Peired, A. J., Weiss, L. A., Katayama, Y., Frenette, P. S. (2004). The integrin {alpha}M{beta}2 anchors hematopoietic progenitors in the bone marrow during enforced mobilization. Blood 104: 993-1001 [Abstract] [Full Text]
Yuan, K., Hong, T.-M., Chen, J. J. W., Tsai, W. H., Lin, M. T. (2004). Syndecan-1 up-regulated by ephrinB2/EphB4 plays dual roles in inflammatory angiogenesis. Blood 104: 1025-1033 [Abstract] [Full Text]
Castelli, R., Porro, F., Tarsia, P. (2004). The heparins and cancer: review of clinical trials and biological properties. Vasc Med 9: 205-213 [Abstract]
Zen, K., Babbin, B. A., Liu, Y., Whelan, J. B., Nusrat, A., Parkos, C. A. (2004). JAM-C Is a Component of Desmosomes and a Ligand for CD11b/CD18-mediated Neutrophil Transepithelial Migration. Mol. Biol. Cell 15: 3926-3937 [Abstract] [Full Text]
Wetzel, A., Chavakis, T., Preissner, K. T., Sticherling, M., Haustein, U.-F., Anderegg, U., Saalbach, A. (2004). Human Thy-1 (CD90) on Activated Endothelial Cells Is a Counterreceptor for the Leukocyte Integrin Mac-1 (CD11b/CD18). J. Immunol. 172: 3850-3859 [Abstract] [Full Text]
Netelenbos, T., van den Born, J., Kessler, F. L., Zweegman, S., Huijgens, P. C., Drager, A. M. (2003). In vitro model for hematopoietic progenitor cell homing reveals endothelial heparan sulfate proteoglycans as direct adhesive ligands. J. Leukoc. Biol. 74: 1035-1044 [Abstract] [Full Text]
Chavakis, T., Santoso, S., Clemetson, K. J., Sachs, U. J. H., Isordia-Salas, I., Pixley, R. A., Nawroth, P. P., Colman, R. W., Preissner, K. T. (2003). High Molecular Weight Kininogen Regulates Platelet-Leukocyte Interactions by Bridging Mac-1 and Glycoprotein Ib. J. Biol. Chem. 278: 45375-45381 [Abstract] [Full Text]
Ehlers, R., Ustinov, V., Chen, Z., Zhang, X., Rao, R., Luscinskas, F. W., Lopez, J., Plow, E., Simon, D. I. (2003). Targeting Platelet-Leukocyte Interactions: Identification of the Integrin Mac-1 Binding Site for the Platelet Counter Receptor Glycoprotein Ib{alpha}. JEM 198: 1077-1088 [Abstract] [Full Text]
GOTTE, M. (2003). Syndecans in inflammation. FASEB J. 17: 575-591 [Abstract] [Full Text]
Yakubenko, V. P., Lishko, V. K., Lam, S. C.-T., Ugarova, T. P. (2002). A Molecular Basis for Integrin alpha Mbeta 2 Ligand Binding Promiscuity. J. Biol. Chem. 277: 48635-48642 [Abstract] [Full Text]
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Gotte, M., Joussen, A. M., Klein, C., Andre, P., Wagner, D. D., Hinkes, M. T., Kirchhof, B., Adamis, A. P., Bernfield, M. (2002). Role of Syndecan-1 in Leukocyte-Endothelial Interactions in the Ocular Vasculature. IOVS 43: 1135-1141 [Abstract] [Full Text]
Forsyth, C. B., Mathews, H. L. (2002). Lymphocyte Adhesion to Candida albicans. Infect. Immun. 70: 517-527 [Abstract] [Full Text]
Liu, Y., Merlin, D., Burst, S. L., Pochet, M., Madara, J. L., Parkos, C. A. (2001). The Role of CD47 in Neutrophil Transmigration. INCREASED RATE OF MIGRATION CORRELATES WITH INCREASED CELL SURFACE EXPRESSION OF CD47. J. Biol. Chem. 276: 40156-40166 [Abstract] [Full Text]
Rezzonico, R., Imbert, V., Chicheportiche, R., Dayer, J.-M. (2001). Ligation of CD11b and CD11c {beta}2 integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1{alpha} (MIP-1{alpha}) and MIP-1{beta} production in primary human monocytes through a pathway dependent on nuclear factor-{kappa}B. Blood 97: 2932-2940 [Abstract] [Full Text]
Smorenburg, S. M., Van Noorden, C. J. F. (2001). The Complex Effects of Heparins on Cancer Progression and Metastasis in Experimental Studies. Pharmacol. Rev. 53: 93-106 [Abstract] [Full Text]
May, R., Machesky, L. (2001). Phagocytosis and the actin cytoskeleton. J. Cell Sci. 114: 1061-1077 [Abstract]
Welt, F. G. P., Edelman, E. R., Simon, D. I., Rogers, C. (2000). Neutrophil, Not Macrophage, Infiltration Precedes Neointimal Thickening in Balloon-Injured Arteries. Arterioscler. Thromb. Vasc. Bio. 20: 2553-2558 [Abstract] [Full Text]
Yanaka, K., Kato, N., Nose, T., Peter, K., Schwarz, M., Nordt, T., Bode, C., Conradt, C., Moser, M., Kubler, W. (2000). Heparin Also Interacts With Selectins and Modulates the Cell Adhesion Process Response. Circulation 102 : e169-e169 [Full Text]
Furuta, G. T., Dzus, A. L., Taylor, C. T., Colgan, S. P. (2000). Parallel induction of epithelial surface-associated chemokine and proteoglycan by cellular hypoxia: implications for neutrophil activation. J. Leukoc. Biol. 68: 251-259 [Abstract] [Full Text]
Simon, D. I., Chen, Z., Xu, H., Li, C. Q., Dong, J.-f., McIntire, L. V., Ballantyne, C. M., Zhang, L., Furman, M. I., Berndt, M. C., Lopez, J. A. (2000). Platelet Glycoprotein Ib{alpha} Is a Counterreceptor for the Leukocyte Integrin Mac-1 (Cd11b/Cd18). JEM 192: 193-204 [Abstract] [Full Text]
Gopalan, P. K., Burns, A. R., Simon, S. I., Sparks, S., McIntire, L. V., Smith, C. W. (2000). Preferential sites for stationary adhesion of neutrophils to cytokine-stimulated HUVEC under flow conditions. J. Leukoc. Biol. 68: 47-57 [Abstract] [Full Text]
Salas, A, Sans, M, Soriano, A, Reverter, J C, Anderson, D C, Pique, J M, Panes, J (2000). Heparin attenuates TNF-alpha induced inflammatory response through a CD11b dependent mechanism. Gut 47: 88-96 [Abstract] [Full Text]
SATO, N., SUZUKI, Y., NISHIO, K., SUZUKI, K., NAOKI, K., TAKESHITA, K., KUDO, H., MIYAO, N., TSUMURA, H., SERIZAWA, H., SUEMATSU, M., YAMAGUCHI, K. (2000). Roles of ICAM-1 for Abnormal Leukocyte Recruitment in the Microcirculation of Bleomycin-induced Fibrotic Lung Injury. Am. J. Respir. Crit. Care Med. 161: 1681-1688 [Abstract] [Full Text]
Peter, K., Schwarz, M., Conradt, C., Nordt, T., Moser, M., Kubler, W., Bode, C. (1999). Heparin Inhibits Ligand Binding to the Leukocyte Integrin Mac-1 (CD11b/CD18). Circulation 100: 1533-1539 [Abstract] [Full Text]
Chamorro, A., Vila, N., Ascaso, C., Blanc, R. (1999). Heparin in Acute Stroke With Atrial Fibrillation: Clinical Relevance of Very Early Treatment. Arch Neurol 56: 1098-1102 [Abstract] [Full Text]
Harler, M. B., Wakshull, E., Filardo, E. J., Albina, J. E., Reichner, J. S. (1999). Promotion of Neutrophil Chemotaxis Through Differential Regulation of {beta}1 and {beta}2 Integrins. J. Immunol. 162: 6792-6799 [Abstract] [Full Text]
Schneider, T., Issekutz, T. B., Issekutz, A. C. (1999). The Role of alpha 4 (CD49d) and beta 2 (CD18) Integrins in Eosinophil and Neutrophil Migration to Allergic Lung Inflammation in the Brown Norway Rat. Am. J. Respir. Cell Mol. Bio. 20: 448-457 [Abstract] [Full Text]
Ianelli, C. J., DeLellis, R., Thorley-Lawson, D. A. (1998). CD48 Binds to Heparan Sulfate on the Surface of Epithelial Cells. J. Biol. Chem. 273: 23367-23375 [Abstract] [Full Text]
Neelamegham, S., Taylor, A. D., Burns, A. R., Smith, C. W., Simon, S. I. (1998). Hydrodynamic Shear Shows Distinct Roles for LFA-1 and Mac-1 in Neutrophil Adhesion to Intercellular Adhesion Molecule-1. Blood 92: 1626-1638 [Abstract] [Full Text]
Taguchi, M., Sampath, D., Koga, T., Castro, M., Look, D. C., Nakajima, S., Holtzman, M. J. (1998). Patterns for RANTES Secretion and Intercellular Adhesion Molecule 1 Expression Mediate Transepithelial T Cell Traffic Based on Analyses In Vitro and In Vivo. JEM 187: 1927-1940 [Abstract] [Full Text]
Balsam, L. B., Liang, T. W., Parkos, C. A. (1998). Functional Mapping of CD11b/CD18 Epitopes Important in Neutrophil-Epithelial Interactions: A Central Role of the I Domain. J. Immunol. 160: 5058-5065 [Abstract] [Full Text]
DiScipio, R. G., Daffern, P. J., Schraufstatter, I. U., Sriramarao, P. (1998). Human Polymorphonuclear Leukocytes Adhere to Complement Factor H Through an Interaction That Involves {alpha}M{beta}2 (CD11b/CD18). J. Immunol. 160: 4057-4066 [Abstract] [Full Text]
Iida, J., Meijne, A. M.�L., Oegema, T. R. Jr., Yednock, T. A., Kovach, N. L., Furcht, L. T., McCarthy, J. B. (1998). A Role of Chondroitin Sulfate Glycosaminoglycan Binding Site in alpha 4beta 1 Integrin-mediated Melanoma Cell Adhesion. J. Biol. Chem. 273: 5955-5962 [Abstract] [Full Text]
Lopez-Rodriguez, C., Botella, L., Corbi, A. L. (1997). CCAAT-Enhancer-binding Proteins (C/EBP) Regulate the Tissue Specific Activity of the CD11c Integrin Gene Promoter Through Functional Interactions with Sp1 Proteins. J. Biol. Chem. 272: 29120-29126 [Abstract] [Full Text]
Parkos, C. A. (1997). I. Neutrophil adhesive interactions with intestinal epithelium. Am. J. Physiol. Gastrointest. Liver Physiol. 273: G763-G768 [Abstract] [Full Text]
Giuffre, L., Cordey, A.-S., Monai, N., Tardy, Y., Schapira, M., Spertini, O. (1997). Monocyte Adhesion to Activated Aortic Endothelium: Role of L-Selectin and Heparan Sulfate Proteoglycans. JCB 136: 945-956 [Abstract] [Full Text]
Patel, H. V., Vyas, A. A., Vyas, K. A., Liu, Y.-S., Chiang, C.-M., Chi, L.-M., Wu, W.-g. (1997). Heparin and Heparan Sulfate Bind to Snake Cardiotoxin. SULFATED OLIGOSACCHARIDES AS A POTENTIAL TARGET FOR CARDIOTOXIN ACTION. J. Biol. Chem. 272: 1484-1492 [Abstract] [Full Text]
Johansson, M., Patarroyo, M, Oberg, F, Siegbahn, A, Nilsson, K (1997). Myeloperoxidase mediates cell adhesion via the alpha M beta 2 integrin (Mac-1, CD11b/CD18). J. Cell Sci. 110: 1133-1139 [Abstract]
Penberthy, T. W., Yanling Jiang, , Graves, D. T. (1997). Leukocyte Adhesion Molecules. CROBM 8: 380-388 [Abstract] [Full Text]
Kainulainen, V., Nelimarkka, L., Jarvelainen, H., Laato, M., Jalkanen, M., Elenius, K. (1996). Suppression of Syndecan-1 Expression in Endothelial Cells by Tumor Necrosis Factor-alpha. J. Biol. Chem. 271: 18759-18766 [Abstract] [Full Text]
Xie, X., Rivier, A.-S., Zakrzewicz, A., Bernimoulin, M., Zeng, X.-L., Wessel, H. P., Schapira, M., Spertini, O. (2000). Inhibition of Selectin-mediated Cell Adhesion and Prevention of Acute Inflammation by Nonanticoagulant Sulfated Saccharides. STUDIES WITH CARBOXYL-REDUCED AND SULFATED HEPARIN AND WITH TRESTATIN A SULFATE. J. Biol. Chem. 275: 34818-34825 [Abstract] [Full Text]
Pucci, M., Fibbi, G., Magnelli, L., Del Rosso, M. (2001). Regulation of Urokinase/Urokinase Receptor Interaction by Heparin-like Glycosaminoglycans. J. Biol. Chem. 276: 4756-4765 [Abstract] [Full Text]