Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway

doi:10.1083/jcb.131.1.57

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Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway

C Itin, R Schindler and HP Hauri
Department of Pharmacology, University of Basel, Switzerland.

ERGIC-53 is a lectin-type membrane protein that continuously recycles between the ER, ER-Golgi intermediate compartment (ERGIC) and the cis- Golgi. To identify the targeting signals that mediate this recycling, N- glycosylated and myc-tagged variants of ERGIC-53 were constructed. By monitoring endoglycosidase H resistance, we measured the loss from the ER-ERGIC-cis-Golgi cycle of ERGIC-53. A domain exchange approach with the plasma membrane reporter protein CD4 showed that the transmembrane and the lumenal domains are not sufficient, while the cytoplasmic domain of ERGIC-53 is required and sufficient for pre-medial-Golgi localization. However, the ERGIC-53 cytoplasmic domain on CD4 lead to increased ER-staining by immunofluorescence microscopy indicating that this domain alone cannot provide for unbiased recycling through the ER- ERGIC-cis-Golgi compartments. Complete progress through the ER-ERGIC- cis-Golgi recycling pathway requires the cytoplasmic domain acting together with the lumenal domain of ERGIC-53. Dissection of the cytoplasmic domain revealed a COOH-terminal di-lysine ER-retrieval signal, KKFF, and an RSQQE targeting determinant adjacent to the transmembrane domain. Surprisingly, the two COOH-terminal phenylalanines influence the targeting. They reduce the ER-retrieval capacity of the di-lysine signal and modulate the RSQQE determinant.
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Nelson, D. S., Alvarez, C., Gao, Y.-s., Garcia-Mata, R., Fialkowski, E., Sztul, E. (1998). The Membrane Transport Factor TAP/p115 Cycles between the Golgi and Earlier Secretory Compartments and Contains Distinct Domains Required for Its Localization and Function. JCB 143: 319-331 [Abstract] [Full Text]
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Schroder-Kohne, S, Letourneur, F, Riezman, H (1998). Alpha-COP can discriminate between distinct, functional di-lysine signals in vitro and regulates access into retrograde transport. J. Cell Sci. 111: 3459-3470 [Abstract]
Klumperman, J, Schweizer, A, Clausen, H, Tang, B., Hong, W, Oorschot, V, Hauri, H. (1998). The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment. J. Cell Sci. 111: 3411-3425 [Abstract]
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