A postprophase topoisomerase II-dependent chromatid core separation step in the formation of metaphase chromosomes

doi:10.1083/jcb.131.1.7

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A postprophase topoisomerase II-dependent chromatid core separation step in the formation of metaphase chromosomes

JF Gimenez-Abian, DJ Clarke, AM Mullinger, CS Downes and RT Johnson
Department of Zoology, University of Cambridge, United Kingdom.

Metaphase chromatids are believed to consist of loops of chromatin anchored to a central scaffold, of which a major component is the decatenatory enzyme DNA topoisomerase II. Silver impregnation selectively stains an axial element of metaphase and anaphase chromatids; but we find that in earlier stages of mitosis, silver staining reveals an initially single, folded midline structure, which separates at prometaphase to form two chromatid axes. Inhibition of topoisomerase II prevents this separation, and also prevents the contraction of chromatids that occurs when metaphase is arrested. Immunolocalization of topoisomerase II alpha reveals chromatid cores analogous to those seen with silver staining. We conclude that the chromatid cores in early mitosis form a single structure, constrained by DNA catenations, which must separate before metaphase chromatids can be resolved.
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