Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway

doi:10.1083/jcb.131.3.591

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Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway

TM Harding, KA Morano, SV Scott and DJ Klionsky
Section of Microbiology, University of California, Davis 95616, USA.

In Saccharomyces cerevisiae the vacuolar protein aminopeptidase I (API) is localized to the vacuole independent of the secretory pathway. The alternate targeting mechanism used by this protein has not been characterized. API is synthesized as a 61-kD soluble cytosolic precursor. Upon delivery to the vacuole, the amino-terminal propeptide is removed by proteinase B (PrB) to yield the mature 50-kD hydrolase. We exploited this delivery-dependent maturation event in a mutant screen to identify genes whose products are involved in API targeting. Using antiserum to the API propeptide, we isolated mutants that accumulate precursor API. These mutants, designated cvt, fall into eight complementation groups, five of which define novel genes. These five complementation groups exhibit a specific defect in maturation of API, but do not have a significant effect on vacuolar protein targeting through the secretory pathway. Localization studies show that precursor API accumulates outside of the vacuole in all five groups, indicating that they are blocked in API targeting and/or translocation. Future analysis of these gene products will provide information about the subcellular components involved in this alternate mechanism of vacuolar protein localization.
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Kirisako, T., Ichimura, Y., Okada, H., Kabeya, Y., Mizushima, N., Yoshimori, T., Ohsumi, M., Takao, T., Noda, T., Ohsumi, Y. (2000). The Reversible Modification Regulates the Membrane-Binding State of Apg8/Aut7 Essential for Autophagy and the Cytoplasm to Vacuole Targeting Pathway. JCB 151: 263-276 [Abstract] [Full Text]
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Lang, T., Reiche, S., Straub, M., Bredschneider, M., Thumm, M. (2000). Autophagy and the cvt Pathway Both Depend on AUT9. J. Bacteriol. 182: 2125-2133 [Abstract] [Full Text]
Price, A., Wickner, W., Ungermann, C. (2000). Proteins Needed for Vesicle Budding from the Golgi Complex Are Also Required for the Docking Step of Homotypic Vacuole Fusion. JCB 148: 1223-1230 [Abstract] [Full Text]
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Noda, T., Kim, J., Huang, W.-P., Baba, M., Tokunaga, C., Ohsumi, Y., Klionsky, D. J. (2000). Apg9p/Cvt7p Is an Integral Membrane Protein Required for Transport Vesicle Formation in the Cvt and Autophagy Pathways. JCB 148: 465-480 [Abstract] [Full Text]
Suriapranata, I, Epple, U., Bernreuther, D, Bredschneider, M, Sovarasteanu, K, Thumm, M (2000). The breakdown of autophagic vesicles inside the vacuole depends on Aut4p. J. Cell Sci. 113: 4025-4033 [Abstract]
Kirisako, T., Baba, M., Ishihara, N., Miyazawa, K., Ohsumi, M., Yoshimori, T., Noda, T., Ohsumi, Y. (1999). Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast. JCB 147: 435-446 [Abstract] [Full Text]
von Mollard, G. F., Stevens, T. H. (1999). The Saccharomyces cerevisiae v-SNARE Vti1p Is Required for Multiple Membrane Transport Pathways to the Vacuole. Mol. Biol. Cell 10: 1719-1732 [Abstract] [Full Text]
Kim, J., Dalton, V. M., Eggerton, K. P., Scott, S. V., Klionsky, D. J. (1999). Apg7p/Cvt2p Is Required for the Cytoplasm-to-Vacuole Targeting, Macroautophagy, and Peroxisome Degradation Pathways. Mol. Biol. Cell 10: 1337-1351 [Abstract] [Full Text]
Yuan, W., Strømhaug, P. E., Dunn, W. A. Jr. (1999). Glucose-induced Autophagy of Peroxisomes in Pichia pastoris Requires a Unique E1-like Protein. Mol. Biol. Cell 10: 1353-1366 [Abstract] [Full Text]
Hutchins, M., Veenhuis, M, Klionsky, D. (1999). Peroxisome degradation in Saccharomyces cerevisiae is dependent on machinery of macroautophagy and the Cvt pathway. J. Cell Sci. 112: 4079-4087 [Abstract]
Morano, K. A., Santoro, N., Koch, K. A., Thiele, D. J. (1999). A trans-Activation Domain in Yeast Heat Shock Transcription Factor Is Essential for Cell Cycle Progression during Stress. Mol. Cell. Biol. 19: 402-411 [Abstract] [Full Text]
Zheng, B., Wu, J. N., Schober, W., Lewis, D. E., Vida, T. (1998). Isolation of yeast mutants defective for localization of vacuolar vital dyes. Proc. Natl. Acad. Sci. USA 95: 11721-11726 [Abstract] [Full Text]
Kametaka, S., Okano, T., Ohsumi, M., Ohsumi, Y. (1998). Apg14p and Apg6/Vps30p Form a Protein Complex Essential for Autophagy in the Yeast, Saccharomyces cerevisiae. J. Biol. Chem. 273: 22284-22291 [Abstract] [Full Text]
Klionsky, D. J. (1998). Nonclassical Protein Sorting to the Yeast Vacuole. J. Biol. Chem. 273: 10807-10810 [Full Text]
Chiang, M.-C., Chiang, H.-L. (1998). Vid24p, a Novel Protein Localized to the Fructose-1,6-bisphosphatase-containing Vesicles, Regulates Targeting of Fructose-1,6-bisphosphatase from the Vesicles to the Vacuole for Degradation. JCB 140: 1347-1356 [Abstract] [Full Text]
Wang, Y.-X., Catlett, N. L., Weisman, L. S. (1998). Vac8p, a Vacuolar Protein with Armadillo Repeats, Functions in both Vacuole Inheritance and Protein Targeting from the Cytoplasm to Vacuole. JCB 140: 1063-1074 [Abstract] [Full Text]
Bryant, N. J., Stevens, T. H. (1998). Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole. Microbiol. Mol. Biol. Rev. 62: 230-247 [Abstract] [Full Text]
Shieh, H.-L., Chiang, H.-L. (1998). In Vitro Reconstitution of Glucose-induced Targeting of Fructose-1,6-bisphosphatase into the Vacuole in Semi-intact Yeast Cells. J. Biol. Chem. 273: 3381-3387 [Abstract] [Full Text]
Fleckenstein, D, Rohde, M, Klionsky, D., Rudiger, M (1998). Yel013p (Vac8p), an armadillo repeat protein related to plakoglobin and importin alpha is associated with the yeast vacuole membrane. J. Cell Sci. 111: 3109-3118 [Abstract]
(1998). Genetic Interaction With vps8-200 Allows Partial Suppression of the Vestigial Vacuole Phenotype Caused by a pep5 Mutation in Saccharomyces cerevisiae. Genetics 148: 71-84
(1998). Pth1/Vam3p Is the Syntaxin Homolog at the Vacuolar Membrane of Saccharomyces cerevisae Required for the Delivery of Vacuolar Hydrolases. Genetics 148: 85-100
Baba, M., Osumi, M., Scott, S. V., Klionsky, D. J., Ohsumi, Y. (1997). Two Distinct Pathways for Targeting Proteins from the Cytoplasm to the Vacuole/Lysosome. JCB 139: 1687-1695 [Abstract] [Full Text]
Stepp, J. D., Huang, K., Lemmon, S. K. (1997). The Yeast Adaptor Protein Complex, AP-3, Is Essential for the Efficient Delivery of Alkaline Phosphatase by the Alternate Pathway to the Vacuole. JCB 139: 1761-1774 [Abstract] [Full Text]
Scott, S. V., Baba, M., Ohsumi, Y., Klionsky, D. J. (1997). Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism. JCB 138: 37-44 [Abstract] [Full Text]
Kim, J., Scott, S. V., Oda, M. N., Klionsky, D. J. (1997). Transport of a Large Oligomeric Protein by the Cytoplasm to Vacuole Protein Targeting Pathway. JCB 137: 609-618 [Abstract] [Full Text]
Nakamura, N., Hirata, A., Ohsumi, Y., Wada, Y. (1997). Vam2/Vps41p and Vam6/Vps39p Are Components of a Protein Complex on the Vacuolar Membranes and Involved in the Vacuolar Assembly in the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 272: 11344-11349 [Abstract] [Full Text]
Huang, P.-H., Chiang, H.-L. (1997). Identification of Novel Vesicles in the Cytosol to Vacuole Protein Degradation Pathway. JCB 136: 803-810 [Abstract] [Full Text]
Harding, T. M., Hefner-Gravink, A., Thumm, M., Klionsky, D. J. (1996). Genetic and Phenotypic Overlap between Autophagy and the Cytoplasm to Vacuole Protein Targeting Pathway. J. Biol. Chem. 271: 17621-17624 [Abstract] [Full Text]
Tomashek, J. J., Sonnenburg, J. L., Artimovich, J. M., Klionsky, D. J. (1996). Resolution of Subunit Interactions and Cytoplasmic Subcomplexes of the Yeast Vacuolar Proton-translocating ATPase. J. Biol. Chem. 271: 10397-10404 [Abstract] [Full Text]
Paz, Y., Elazar, Z., Fass, D. (2000). Structure of GATE-16, Membrane Transport Modulator and Mammalian Ortholog of Autophagocytosis Factor Aut7p. J. Biol. Chem. 275: 25445-25450 [Abstract] [Full Text]
Teter, S. A., Eggerton, K. P., Scott, S. V., Kim, J., Fischer, A. M., Klionsky, D. J. (2001). Degradation of Lipid Vesicles in the Yeast Vacuole Requires Function of Cvt17, a Putative Lipase. J. Biol. Chem. 276: 2083-2087 [Abstract] [Full Text]
Shieh, H.-L., Chen, Y., Brown, C. R., Chiang, H.-L. (2001). Biochemical Analysis of Fructose-1,6-bisphosphatase Import into Vacuole Import and Degradation Vesicles Reveals a Role for UBC1 in Vesicle Biogenesis. J. Biol. Chem. 276: 10398-10406 [Abstract] [Full Text]
Scott, S. V., Nice, D. C. III, Nau, J. J., Weisman, L. S., Kamada, Y., Keizer-Gunnink, I., Funakoshi, T., Veenhuis, M., Ohsumi, Y., Klionsky, D. J. (2000). Apg13p and Vac8p Are Part of a Complex of Phosphoproteins That Are Required for Cytoplasm to Vacuole Targeting. J. Biol. Chem. 275: 25840-25849 [Abstract] [Full Text]
Andrei-Selmer, C., Knuppel, A., Satyanarayana, C., Heese, C., Schu, P. V. (2001). A New Class of Mutants Deficient in Dodecamerization of Aminopeptidase 1 and Vacuolar Transport. J. Biol. Chem. 276: 11606-11614 [Abstract] [Full Text]
Hutchins, M. U., Klionsky, D. J. (2001). Vacuolar Localization of Oligomeric alpha -Mannosidase Requires the Cytoplasm to Vacuole Targeting and Autophagy Pathway Components in Saccharomyces cerevisiae. J. Biol. Chem. 276: 20491-20498 [Abstract] [Full Text]
Wang, C.-W., Kim, J., Huang, W.-P., Abeliovich, H., Stromhaug, P. E., Dunn, W. A. Jr., Klionsky, D. J. (2001). Apg2 Is a Novel Protein Required for the Cytoplasm to Vacuole Targeting, Autophagy, and Pexophagy Pathways. J. Biol. Chem. 276: 30442-30451 [Abstract] [Full Text]