Identification of the chromosome localization domain of the Drosophila nod kinesin-like protein

doi:10.1083/jcb.131.4.833

This Article

	Full Text (PDF, 7870K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Afshar, K.
	Articles by Hawley, R. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Afshar, K.
	Articles by Hawley, R. S.


Social Bookmarking

	What's this?

ARTICLES

Identification of the chromosome localization domain of the Drosophila nod kinesin-like protein

K Afshar, J Scholey and RS Hawley
Section of Molecular and Cellular Biology, University of California at Davis 95616, USA.

The nod kinesin-like protein is localized along the arms of meiotic chromosomes and is required to maintain the position of achiasmate chromosomes on the developing meiotic spindle. Here we show that the localization of ectopically expressed nod protein on mitotic chromosomes precisely parallels that observed for wild-type nod protein on meiotic chromosomes. Moreover, the carboxyl-terminal half of the nod protein also binds to chromosomes when overexpressed in mitotic cells, whereas the overexpressed amino-terminal motor domain binds only to microtubules. Chromosome localization of the carboxyl-terminal domain of nod depends upon an 82-amino acid region comprised of three copies of a sequence homologous to the DNA-binding domain of HMG 14/17 proteins. These data map the two primary functional domains of the nod protein in vivo and provide a molecular explanation for the directing of the nod protein to a specific subcellular component, the chromosome.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Anderson, J. A., Gilliland, W. D., Langley, C. H. (2009). Molecular Population Genetics and Evolution of Drosophila Meiosis Genes. Genetics 181: 177-185 [Abstract] [Full Text]
Cui, W., Hawley, R. S. (2005). The HhH(2)/NDD Domain of the Drosophila Nod Chromokinesin-like Protein Is Required for Binding to Chromosomes in the Oocyte Nucleus. Genetics 171: 1823-1835 [Abstract] [Full Text]
Cui, W., Sproul, L. R., Gustafson, S. M., Matthies, H. J.G., Gilbert, S. P., Hawley, R. S. (2005). Drosophila Nod Protein Binds Preferentially to the Plus Ends of Microtubules and Promotes Microtubule Polymerization In Vitro. Mol. Biol. Cell 16: 5400-5409 [Abstract] [Full Text]
Powers, J., Rose, D. J., Saunders, A., Dunkelbarger, S., Strome, S., Saxton, W. M. (2004). Loss of KLP-19 polar ejection force causes misorientation and missegregation of holocentric chromosomes. JCB 166: 991-1001 [Abstract] [Full Text]
Koehler, K. E., Millie, E. A., Cherry, J. P., Schrump, S. E., Hassold, T. J. (2004). Meiotic Exchange and Segregation in Female Mice Heterozygous for Paracentric Inversions. Genetics 166: 1199-1214 [Abstract] [Full Text]
Lu, C., Srayko, M., Mains, P. E. (2004). The Caenorhabditis elegans Microtubule-severing Complex MEI-1/MEI-2 Katanin Interacts Differently with Two Superficially Redundant {beta}-Tubulin Isotypes. Mol. Biol. Cell 15: 142-150 [Abstract] [Full Text]
Harris, D., Orme, C., Kramer, J., Namba, L., Champion, M., Palladino, M. J., Natzle, J., Hawley, R. S. (2003). A Deficiency Screen of the Major Autosomes Identifies a Gene (matrimony) That Is Haplo-insufficient for Achiasmate Segregation in Drosophila Oocytes. Genetics 165: 637-652 [Abstract] [Full Text]
Matthies, H. J.G., Baskin, R. J., Hawley, R. S. (2001). Orphan Kinesin NOD Lacks Motile Properties But Does Possess a Microtubule-stimulated ATPase Activity. Mol. Biol. Cell 12: 4000-4012 [Abstract] [Full Text]
Chan, H.Y. E., Brogna, S., O'Kane, C. J. (2001). Dribble, the Drosophila KRR1p Homologue, Is Involved in rRNA Processing. Mol. Biol. Cell 12: 1409-1419 [Abstract] [Full Text]
Afshar, K., Gönczy, P., DiNardo, S., Wasserman, S. A. (2001). fumble Encodes a Pantothenate Kinase Homolog Required for Proper Mitosis and Meiosis in Drosophila melanogaster. Genetics 157: 1267-1276 [Abstract] [Full Text]
Matthies, H. J.G., Messina, L. G., Namba, R., Greer, K. J., Walker, M.Y., Hawley, R. S. (1999). Mutations in the {alpha}-Tubulin 67C Gene Specifically Impair Achiasmate Segregation in Drosophila melanogaster. JCB 147: 1137-1144 [Abstract] [Full Text]
Zwick, M. E., Salstrom, J. L., Langley, C. H. (1999). Genetic Variation in Rates of Nondisjunction: Association of Two Naturally Occurring Polymorphisms in the Chromokinesin nod With Increased Rates of Nondisjunction in Drosophila melanogaster. Genetics 152: 1605-1614 [Abstract] [Full Text]
Zwick, M. E., Cutler, D. J., Langley, C. H. (1999). Classic Weinstein: Tetrad Analysis, Genetic Variation and Achiasmate Segregation in Drosophila and Humans. Genetics 152: 1615-1629 [Abstract] [Full Text]
Manning, B. D., Barrett, J. G., Wallace, J. A., Granok, H., Snyder, M. (1999). Differential Regulation of the Kar3p Kinesin-related Protein by Two Associated Proteins, Cik1p and Vik1p. JCB 144: 1219-1233 [Abstract] [Full Text]
Wittmann, T., Boleti, H., Antony, C., Karsenti, E., Vernos, I. (1998). Localization of the Kinesin-like Protein Xklp2 to Spindle Poles Requires a Leucine Zipper, a Microtubule-associated Protein, and Dynein. JCB 143: 673-685 [Abstract] [Full Text]
Molina, I., Baars, S., Brill, J. A., Hales, K. G., Fuller, M. T., Ripoll, P. (1997). A Chromatin-associated Kinesin-related Protein Required for Normal Mitotic Chromosome Segregation in Drosophila. JCB 139: 1361-1371 [Abstract] [Full Text]
Clark, I., Jan, L., Jan, Y. (1997). Reciprocal localization of Nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle. Development 124: 461-470 [Abstract]