Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells

doi:10.1083/jcb.131.6.1649

This Article

	Full Text (PDF, 3719K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Jacobson, M. R.
	Articles by Pederson, T.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Jacobson, M. R.
	Articles by Pederson, T.


Social Bookmarking

	What's this?

ARTICLES

Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells

MR Jacobson, LG Cao, YL Wang and T Pederson
Cell Biology Group, Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545, USA.

The dynamic intra-nuclear localization of MRP RNA, the RNA component of the ribonucleoprotein enzyme RNase MRP, was examined in living cells by the method of fluorescent RNA cytochemistry (Wang, J., L.-G. Cao, Y.-L. Wang, and T. Pederson. 1991. Proc. Natl. Acad. Sci. USA. 88:7391-7395). MRP RNA very rapidly accumulated in nucleoli after nuclear microinjection of normal rat kidney (NRK) epithelial cells. Localization was specifically in the dense fibrillar component of the nucleolus, as revealed by immunocytochemistry with a monoclonal antibody against fibrillarin, a known dense fibrillar component protein, as well as by digital optical sectioning microscopy and 3-D stereo reconstruction. When MRP RNA was injected into the cytoplasm it was not imported into the nucleus. Nuclear microinjection of mutant MRP RNAs revealed that nucleolar localization requires a sequence element (nucleotides 23-62) previously implicated as a binding site for a nucleolar protein, the To antigen. These results demonstrate the dynamic localization of MRP RNA in the nucleus and provide important insights into the nucleolar targeting of MRP RNA.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Novoselov, S. V., Kryukov, G. V., Xu, X.-M., Carlson, B. A., Hatfield, D. L., Gladyshev, V. N. (2007). Selenoprotein H Is a Nucleolar Thioredoxin-like Protein with a Unique Expression Pattern. J. Biol. Chem. 282: 11960-11968 [Abstract] [Full Text]
Welting, T. J.M., Peters, F. M.A., Hensen, S. M.M., van Doorn, N. L., Kikkert, B. J., Raats, J. M.H., van Venrooij, W. J., Pruijn, G. J.M. (2007). Heterodimerization regulates RNase MRP/RNase P association, localization, and expression of Rpp20 and Rpp25. RNA 13: 65-75 [Abstract] [Full Text]
Christensen, M. O., Krokowski, R. M., Barthelmes, H. U., Hock, R., Boege, F., Mielke, C. (2004). Distinct Effects of Topoisomerase I and RNA Polymerase I Inhibitors Suggest a Dual Mechanism of Nucleolar/Nucleoplasmic Partitioning of Topoisomerase I. J. Biol. Chem. 279: 21873-21882 [Abstract] [Full Text]
Qi, Y., Ding, B. (2003). Differential Subnuclear Localization of RNA Strands of Opposite Polarity Derived from an Autonomously Replicating Viroid. Plant Cell 15: 2566-2577 [Abstract] [Full Text]
Kuijpers, T W, Ridanpaa, M, Peters, M, de Boer, I, Vossen, J M J J, Pals, S T, Kaitila, I, Hennekam, R C M (2003). Short-limbed dwarfism with bowing, combined immune deficiency, and late onset aplastic anaemia caused by novel mutations in the RMPR gene. J. Med. Genet. 40: 761-766 [Full Text]
Politz, J. C., Lewandowski, L. B., Pederson, T. (2002). Signal recognition particle RNA localization within the nucleolus differs from the classical sites of ribosome synthesis. JCB 159: 411-418 [Abstract] [Full Text]
Li, Y., Altman, S. (2002). Partial reconstitution of human RNase P in HeLa cells between its RNA subunit with an affinity tag and the intact protein components. Nucleic Acids Res 30: 3706-3711 [Abstract] [Full Text]
Houser-Scott, F., Xiao, S., Millikin, C. E., Zengel, J. M., Lindahl, L., Engelke, D. R. (2002). Interactions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase P. Proc. Natl. Acad. Sci. USA 99: 2684-2689 [Abstract] [Full Text]
van Eenennaam, H., van der Heijden, A., Janssen, R. J. R. J., van Venrooij, W. J., Pruijn, G. J. M. (2001). Basic Domains Target Protein Subunits of the RNase MRP Complex to the Nucleolus Independently of Complex Association. Mol. Biol. Cell 12: 3680-3689 [Abstract] [Full Text]
Maraia, R. J. (2001). La Protein and the Trafficking of Nascent RNA Polymerase III Transcripts. JCB 153: f13-f18 [Full Text]
Pederson, T. (2001). Fluorescent RNA cytochemistry: tracking gene transcripts in living cells. Nucleic Acids Res 29: 1013-1016 [Abstract] [Full Text]
(2001). Prespliceosomal Assembly on Microinjected Precursor mRNA Takes Place in Nuclear Speckles. Mol. Biol. Cell 12: 393-406 [Abstract] [Full Text]
GERBI, S.A., BOROVJAGIN, A.V., EZROKHI, M., LANGE, T.S. (2001). Ribosome Biogenesis: Role of Small Nucleolar RNA in Maturation of Eukaryotic rRNA. Cold Spring Harb Symp Quant Biol 66: 575-590 [Abstract]
Lange, T. S., Gerbi, S. A. (2000). Transient Nucleolar Localization Of U6 Small Nuclear RNA In Xenopus Laevis Oocytes. Mol. Biol. Cell 11: 2419-2428 [Abstract] [Full Text]
Pederson, T., Politz, J. C. (2000). The Nucleolus and the Four Ribonucleoproteins of Translation. JCB 148: 1091-1096 [Full Text]
Jady, B. E., Kiss, T. (2000). Characterisation of the U83 and U84 small nucleolar RNAs: two novel 2'-O-ribose methylation guide RNAs that lack complementarities to ribosomal RNAs. Nucleic Acids Res 28: 1348-1354 [Abstract] [Full Text]
Politz, J. C., Yarovoi, S., Kilroy, S. M., Gowda, K., Zwieb, C., Pederson, T. (2000). Signal recognition particle components in the nucleolus. Proc. Natl. Acad. Sci. USA 97: 55-60 [Abstract] [Full Text]
PEDERSON, T. (1999). Movement and localization of RNA in the cell nucleus. FASEB J. 13: 238S-242S [Abstract] [Full Text]
Cai, T., Reilly, T. R., Cerio, M., Schmitt, M. E. (1999). Mutagenesis of SNM1, Which Encodes a Protein Component of the Yeast RNase MRP, Reveals a Role for This Ribonucleoprotein Endoribonuclease in Plasmid Segregation. Mol. Cell. Biol. 19: 7857-7869 [Abstract] [Full Text]
Lange, T. S., Ezrokhi, M., Amaldi, F., Gerbi, S. A. (1999). Box H and Box ACA Are Nucleolar Localization Elements of U17 Small Nucleolar RNA. Mol. Biol. Cell 10: 3877-3890 [Abstract] [Full Text]
Ganot, P., Jady, B. E., Bortolin, M.-L., Darzacq, X., Kiss, T. (1999). Nucleolar Factors Direct the 2'-O-Ribose Methylation and Pseudouridylation of U6 Spliceosomal RNA. Mol. Cell. Biol. 19: 6906-6917 [Abstract] [Full Text]
Jarrous, N., Wolenski, J. S., Wesolowski, D., Lee, C., Altman, S. (1999). Localization in the Nucleolus and Coiled Bodies of Protein Subunits of the Ribonucleoprotein Ribonuclease P. JCB 146: 559-572 [Abstract] [Full Text]
Huang, S., Deerinck, T. J., Ellisman, M. H., Spector, D. L. (1998). The Perinucleolar Compartment and Transcription. JCB 143: 35-47 [Abstract] [Full Text]
Lange, T. S., Ezrokhi, M., Borovjagin, A. V., Rivera-León, R., North, M. T., Gerbi, S. A. (1998). Nucleolar Localization Elements of Xenopus laevis U3 Small Nucleolar RNA. Mol. Biol. Cell 9: 2973-2985 [Abstract] [Full Text]
Jacobson, M. R., Pederson, T. (1998). Localization of signal recognition particle RNA in the nucleolus of mammalian cells. Proc. Natl. Acad. Sci. USA 95: 7981-7986 [Abstract] [Full Text]
Chamberlain, J. R., Lee, Y., Lane, W. S., Engelke, D. R. (1998). Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase�MRP. Genes Dev. 12: 1678-1690 [Abstract] [Full Text]
Eder, P.�S., Kekuda, R., Stolc, V., Altman, S. (1997). Characterization of two scleroderma autoimmune antigens that copurify with human ribonuclease�P. Proc. Natl. Acad. Sci. USA 94: 1101-1106 [Abstract] [Full Text]
Jacobson, M., Cao, L., Taneja, K, Singer, R., Wang, Y., Pederson, T (1997). Nuclear domains of the RNA subunit of RNase P. J. Cell Sci. 110: 829-837 [Abstract]