Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by alpha 1 beta 1 and alpha 2 beta 1 integrins

doi:10.1083/jcb.131.6.1903

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 2098K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Langholz, O.
	Articles by Eckes, B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Langholz, O.
	Articles by Eckes, B.


Social Bookmarking

	What's this?

ARTICLES

Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by alpha 1 beta 1 and alpha 2 beta 1 integrins

O Langholz, D Rockel, C Mauch, E Kozlowska, I Bank, T Krieg and B Eckes
Department of Dermatology, University of Cologne, Germany.

The reorganization of extracellular matrix (ECM) is an important function in many biological and pathophysiological processes. Culture of fibroblasts in a three-dimensional collagenous environment represents a suitable system to study the underlying mechanisms resulting from cell-ECM interaction, which leads to reprogramming of fibroblast biosynthetic capacity. The aim of this study was to identify receptors that transduce ECM signals into cellular events, resulting in reprogramming of connective tissue metabolism. Our data demonstrate that in human skin fibroblasts alpha 1 beta 1 and alpha 2 beta 1 integrins are the major receptors responsible for regulating ECM remodeling: alpha 1 beta 1 mediates the signals inducing downregulation of collagen gene expression, whereas the alpha 2 beta 1 integrin mediates induction of collagenase (MMP-1). Applying mAb directed against different integrin subunits resulted in triggering the heterodimeric receptors and enhancing the normal biochemical response to receptor ligation. Different signal transduction inhibitors were tested for their influence on gel contraction, expression of alpha 1(I) collagen and MMP-1 in fibroblasts within collagen gels. Ortho-vanadate and herbimycin A displayed no significant effect on any of these three processes. In contrast, genistein reduced lattice contraction, and completely inhibited induction of MMP-1, whereas type I collagen down- regulation was unaltered. Calphostin C inhibited only lattice contraction. Taken together, these data indicate a role of tyrosine- specific protein kinases in mediating gel contraction and induction of MMP-1, as well as an involvement of protein kinase C in the contraction process. The data presented here indicate that different signaling pathways exist leading to the three events discussed here, and that these pathways do not per se depend upon each other.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Morales, S. A., Mareninov, S., Wadehra, M., Zhang, L., Goodglick, L., Braun, J., Gordon, L. K. (2009). FAK Activation and the Role of Epithelial Membrane Protein 2 (EMP2) in Collagen Gel Contraction. IOVS 50: 462-469 [Abstract] [Full Text]
Peng, Y.-W., Zallocchi, M., Meehan, D. T., Delimont, D., Chang, B., Hawes, N., Wang, W., Cosgrove, D. (2008). Progressive Morphological and Functional Defects in Retinas from {alpha}1 Integrin-Null Mice. IOVS 49: 4647-4654 [Abstract] [Full Text]
Henneman, S., Von den Hoff, J. W., Maltha, J. C. (2008). Mechanobiology of tooth movement. Eur J Orthod 30: 299-306 [Abstract] [Full Text]
Huet, E., Vallee, B., Szul, D., Verrecchia, F., Mourah, S., Jester, J. V., Hoang-Xuan, T., Menashi, S., Gabison, E. E. (2008). Extracellular matrix metalloproteinase inducer/CD147 promotes myofibroblast differentiation by inducing {alpha}-smooth muscle actin expression and collagen gel contraction: implications in tissue remodeling. FASEB J. 22: 1144-1154 [Abstract] [Full Text]
Ferri, N., Colombo, G., Ferrandi, C., Raines, E. W., Levkau, B., Corsini, A. (2007). Simvastatin Reduces MMP1 Expression in Human Smooth Muscle Cells Cultured on Polymerized Collagen by Inhibiting Rac1 Activation. Arterioscler. Thromb. Vasc. Bio. 27: 1043-1049 [Abstract] [Full Text]
Kong, H. J., Boontheekul, T., Mooney, D. J. (2006). Quantifying the relation between adhesion ligand-receptor bond formation and cell phenotype. Proc. Natl. Acad. Sci. USA 103: 18534-18539 [Abstract] [Full Text]
McBrien, N. A., Metlapally, R., Jobling, A. I., Gentle, A. (2006). Expression of Collagen-Binding Integrin Receptors in the Mammalian Sclera and Their Regulation during the Development of Myopia. IOVS 47: 4674-4682 [Abstract] [Full Text]
Zhang, Z.-G., Bothe, I., Hirche, F., Zweers, M., Gullberg, D., Pfitzer, G., Krieg, T., Eckes, B., Aumailley, M. (2006). Interactions of primary fibroblasts and keratinocytes with extracellular matrix proteins: contribution of {alpha}2{beta}1 integrin.. J. Cell Sci. 119: 1886-1895 [Abstract] [Full Text]
Franco, C., Ho, B., Mulholland, D., Hou, G., Islam, M., Donaldson, K., Bendeck, M. P. (2006). Doxycycline Alters Vascular Smooth Muscle Cell Adhesion, Migration, and Reorganization of Fibrillar Collagen Matrices. Am. J. Pathol. 168: 1697-1709 [Abstract] [Full Text]
Ng, C. P., Hinz, B., Swartz, M. A. (2005). Interstitial fluid flow induces myofibroblast differentiation and collagen alignment in vitro. J. Cell Sci. 118: 4731-4739 [Abstract] [Full Text]
de Giorgio-Miller, A., Bottoms, S., Laurent, G., Carmeliet, P., Herrick, S. (2005). Fibrin-Induced Skin Fibrosis in Mice Deficient in Tissue Plasminogen Activator. Am. J. Pathol. 167: 721-732 [Abstract] [Full Text]
Schapira, K., Lutgens, E., de Fougerolles, A., Sprague, A., Roemen, A., Gardner, H., Koteliansky, V., Daemen, M., Heeneman, S. (2005). Genetic Deletion or Antibody Blockade of {alpha}1{beta}1 Integrin Induces a Stable Plaque Phenotype in ApoE-/- Mice. Arterioscler. Thromb. Vasc. Bio. 25: 1917-1924 [Abstract] [Full Text]
Gratton, M. A., Rao, V. H., Meehan, D. T., Askew, C., Cosgrove, D. (2005). Matrix Metalloproteinase Dysregulation in the Stria Vascularis of Mice with Alport Syndrome: Implications for Capillary Basement Membrane Pathology. Am. J. Pathol. 166: 1465-1474 [Abstract] [Full Text]
Deroanne, C. F., Hamelryckx, D., Ho, T. T. G., Lambert, C. A., Catroux, P., Lapiere, C. M., Nusgens, B. V. (2005). Cdc42 downregulates MMP-1 expression by inhibiting the ERK1/2 pathway. J. Cell Sci. 118: 1173-1183 [Abstract] [Full Text]
Kapyla, J., Jaalinoja, J., Tulla, M., Ylostalo, J., Nissinen, L., Viitasalo, T., Vehvilainen, P., Marjomaki, V., Nykvist, P., Saamanen, A.-M., Farndale, R. W., Birk, D. E., Ala-Kokko, L., Heino, J. (2004). The Fibril-associated Collagen IX Provides a Novel Mechanism for Cell Adhesion to Cartilaginous Matrix. J. Biol. Chem. 279: 51677-51687 [Abstract] [Full Text]
Jokinen, J., Dadu, E., Nykvist, P., Kapyla, J., White, D. J., Ivaska, J., Vehvilainen, P., Reunanen, H., Larjava, H., Hakkinen, L., Heino, J. (2004). Integrin-mediated Cell Adhesion to Type I Collagen Fibrils. J. Biol. Chem. 279: 31956-31963 [Abstract] [Full Text]
Shegogue, D., Trojanowska, M. (2004). Mammalian Target of Rapamycin Positively Regulates Collagen Type I Production via a Phosphatidylinositol 3-Kinase-independent Pathway. J. Biol. Chem. 279: 23166-23175 [Abstract] [Full Text]
Ferri, N., Carragher, N. O., Raines, E. W. (2004). Role of Discoidin Domain Receptors 1 and 2 in Human Smooth Muscle Cell-Mediated Collagen Remodeling: Potential Implications in Atherosclerosis and Lymphangioleiomyomatosis. Am. J. Pathol. 164: 1575-1585 [Abstract] [Full Text]
Asano, Y., Ihn, H., Yamane, K., Kubo, M., Tamaki, K. (2004). Increased Expression Levels of Integrin {alpha}v{beta}5 on Scleroderma Fibroblasts. Am. J. Pathol. 164: 1275-1292 [Abstract] [Full Text]
Wolf, K., Muller, R., Borgmann, S., Brocker, Eva.-B., Friedl, P. (2003). Amoeboid shape change and contact guidance: T-lymphocyte crawling through fibrillar collagen is independent of matrix remodeling by MMPs and other proteases. Blood 102: 3262-3269 [Abstract] [Full Text]
Li, S., Van Den Diepstraten, C., D'Souza, S. J., Chan, B. M. C., Pickering, J. G. (2003). Vascular Smooth Muscle Cells Orchestrate the Assembly of Type I Collagen via {alpha}2{beta}1 Integrin, RhoA, and Fibronectin Polymerization. Am. J. Pathol. 163: 1045-1056 [Abstract] [Full Text]
Sweeney, S. M., DiLullo, G., Slater, S. J., Martinez, J., Iozzo, R. V., Lauer-Fields, J. L., Fields, G. B., Antonio, J. D. S. (2003). Angiogenesis in Collagen I Requires {alpha}2{beta}1 Ligation of a GFP*GER Sequence and Possibly p38 MAPK Activation and Focal Adhesion Disassembly. J. Biol. Chem. 278: 30516-30524 [Abstract] [Full Text]
Ferri, N., Garton, K. J., Raines, E. W. (2003). An NF-{kappa}B-dependent Transcriptional Program Is Required for Collagen Remodeling by Human Smooth Muscle Cells. J. Biol. Chem. 278: 19757-19764 [Abstract] [Full Text]
Kitsiou, P. V., Tzinia, A. K., Stetler-Stevenson, W. G., Michael, A. F., Fan, W.-W., Zhou, B., Tsilibary, E. C. (2003). Glucose-induced changes in integrins and matrix-related functions in cultured human glomerular epithelial cells. Am. J. Physiol. Renal Physiol. 284: F671-F679 [Abstract] [Full Text]
Wolf, K., Mazo, I., Leung, H., Engelke, K., von Andrian, U. H., Deryugina, E. I., Strongin, A. Y., Brocker, E.-B., Friedl, P. (2003). Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis. JCB 160: 267-277 [Abstract] [Full Text]
Whelan, M. C., Senger, D. R. (2003). Collagen I Initiates Endothelial Cell Morphogenesis by Inducing Actin Polymerization through Suppression of Cyclic AMP and Protein Kinase A. J. Biol. Chem. 278: 327-334 [Abstract] [Full Text]
Zigrino, P., Kamiguti, A. S., Eble, J., Drescher, C., Nischt, R., Fox, J. W., Mauch, C. (2002). The Reprolysin Jararhagin, a Snake Venom Metalloproteinase, Functions as a Fibrillar Collagen Agonist Involved in Fibroblast Cell Adhesion and Signaling. J. Biol. Chem. 277: 40528-40535 [Abstract] [Full Text]
Velling, T., Risteli, J., Wennerberg, K., Mosher, D. F., Johansson, S. (2002). Polymerization of Type I and III Collagens Is Dependent On Fibronectin and Enhanced By Integrins alpha 11beta 1 and alpha 2beta 1. J. Biol. Chem. 277: 37377-37381 [Abstract] [Full Text]
Ekholm, E., Hankenson, K. D., Uusitalo, H., Hiltunen, A., Gardner, H., Heino, J., Penttinen, R. (2002). Diminished Callus Size and Cartilage Synthesis in {alpha}1{beta}1 Integrin-Deficient Mice during Bone Fracture Healing. Am. J. Pathol. 160: 1779-1785 [Abstract] [Full Text]
Holtkotter, O., Nieswandt, B., Smyth, N., Muller, W., Hafner, M., Schulte, V., Krieg, T., Eckes, B. (2002). Integrin alpha 2-Deficient Mice Develop Normally, Are Fertile, but Display Partially Defective Platelet Interaction with Collagen. J. Biol. Chem. 277: 10789-10794 [Abstract] [Full Text]
Tulla, M., Pentikainen, O. T., Viitasalo, T., Kapyla, J., Impola, U., Nykvist, P., Nissinen, L., Johnson, M. S., Heino, J. (2001). Selective Binding of Collagen Subtypes by Integrin alpha 1I, alpha 2I, and alpha 10I Domains. J. Biol. Chem. 276: 48206-48212 [Abstract] [Full Text]
Chen, C.-C., Mo, F.-E, Lau, L. F. (2001). The Angiogenic Factor Cyr61 Activates a Genetic Program for Wound Healing in Human Skin Fibroblasts. J. Biol. Chem. 276: 47329-47337 [Abstract] [Full Text]
Klekotka, P. A., Santoro, S. A., Ho, A., Dowdy, S. F., Zutter, M. M. (2001). Mammary Epithelial Cell-Cycle Progression via the {alpha}2{beta}1 Integrin : Unique and Synergistic Roles of the {alpha}2 Cytoplasmic Domain. Am. J. Pathol. 159: 983-992 [Abstract] [Full Text]
Varani, J., Spearman, D., Perone, P., Fligiel, S. E. G., Datta, S. C., Wang, Z. Q., Shao, Y., Kang, S., Fisher, G. J., Voorhees, J. J. (2001). Inhibition of Type I Procollagen Synthesis by Damaged Collagen in Photoaged Skin and by Collagenase-Degraded Collagen in Vitro. Am. J. Pathol. 158: 931-942 [Abstract] [Full Text]
Steffensen, B., Hakkinen, L., Larjava, H. (2001). Proteolytic Events of Wound-Healing -- Coordinated Interactions Among Matrix Metalloproteinases (MMPs), Integrins, and Extracellular Matrix Molecules. CROBM 12: 373-398 [Abstract] [Full Text]
Garnotel, R., Rittie, L., Poitevin, S., Monboisse, J.-C., Nguyen, P., Potron, G., Maquart, F.-X., Randoux, A., Gillery, P. (2000). Human Blood Monocytes Interact with Type I Collagen Through {alpha}x{beta}2 Integrin (CD11c-CD18, gp150-95). J. Immunol. 164: 5928-5934 [Abstract] [Full Text]
Nykvist, P., Tu, H., Ivaska, J., Kapyla, J., Pihlajaniemi, T., Heino, J. (2000). Distinct Recognition of Collagen Subtypes by alpha 1beta 1 and alpha 2beta 1 Integrins. alpha 1beta 1 MEDIATES CELL ADHESION TO TYPE XIII COLLAGEN. J. Biol. Chem. 275: 8255-8261 [Abstract] [Full Text]
Nakamoto, T., Yamagata, T., Sakai, R., Ogawa, S., Honda, H., Ueno, H., Hirano, N., Yazaki, Y., Hirai, H. (2000). CIZ, a Zinc Finger Protein That Interacts with p130cas and Activates the Expression of Matrix Metalloproteinases. Mol. Cell. Biol. 20: 1649-1658 [Abstract] [Full Text]
Vogel, W., Brakebusch, C., Fassler, R., Alves, F., Ruggiero, F., Pawson, T. (2000). Discoidin Domain Receptor 1 Is Activated Independently of beta 1 Integrin. J. Biol. Chem. 275: 5779-5784 [Abstract] [Full Text]
Hou, G., Mulholland, D., Gronska, M. A., Bendeck, M. P. (2000). Type VIII Collagen Stimulates Smooth Muscle Cell Migration and Matrix Metalloproteinase Synthesis after Arterial Injury. Am. J. Pathol. 156: 467-476 [Abstract] [Full Text]
Fluck, M, Tunc-Civelek, V, Chiquet, M (2000). Rapid and reciprocal regulation of tenascin-C and tenascin-Y expression by loading of skeletal muscle. J. Cell Sci. 113: 3583-3591 [Abstract]
Cooke, M., Sakai, T, Mosher, D. (2000). Contraction of collagen matrices mediated by alpha2beta1A and alpha(v)beta3 integrins. J. Cell Sci. 113: 2375-2383 [Abstract]
Aumailley, M, Pesch, M, Tunggal, L, Gaill, F, Fassler, R (2000). Altered synthesis of laminin 1 and absence of basement membrane component deposition in (beta)1 integrin-deficient embryoid bodies. J. Cell Sci. 113: 259-268 [Abstract]
Ivaska, J., Reunanen, H., Westermarck, J., Koivisto, L., Kahari, V.-M., Heino, J. (1999). Integrin {alpha}2{beta}1 Mediates Isoform-Specific Activation of p38 and Upregulation of Collagen Gene Transcription by a Mechanism Involving the {alpha}2 Cytoplasmic Tail. JCB 147: 401-416 [Abstract] [Full Text]
Esparza, J., Vilardell, C., Calvo, J., Juan, M., Vives, J., Urbano-Marquez, A., Yague, J., Cid, M. C. (1999). Fibronectin Upregulates Gelatinase B (MMP-9) and Induces Coordinated Expression of Gelatinase A (MMP-2) and Its Activator MT1-MMP (MMP-14) by Human T Lymphocyte Cell Lines. A Process Repressed Through RAS/MAP Kinase Signaling Pathways. Blood 94: 2754-2766 [Abstract] [Full Text]
Zutter, M. M., Santoro, S. A., Wu, J. E., Wakatsuki, T., Dickeson, S. K., Elson, E. L. (1999). Collagen Receptor Control of Epithelial Morphogenesis and Cell Cycle Progression. Am. J. Pathol. 155: 927-940 [Abstract] [Full Text]
Li, S., Sims, S., Jiao, Y., Chow, L. H., Pickering, J. G. (1999). Evidence From a Novel Human Cell Clone That Adult Vascular Smooth Muscle Cells Can Convert Reversibly Between Noncontractile and Contractile Phenotypes. Circ. Res. 85: 338-348 [Abstract] [Full Text]
Ravanti, L., Heino, J., Lopez-Otin, C., Kahari, V.-M. (1999). Induction of Collagenase-3 (MMP-13) Expression in Human Skin Fibroblasts by Three-dimensional Collagen Is Mediated by p38 Mitogen-activated Protein Kinase. J. Biol. Chem. 274: 2446-2455 [Abstract] [Full Text]
Grinnell, F., Ho, C.-H., Lin, Y.-C., Skuta, G. (1999). Differences in the Regulation of Fibroblast Contraction of Floating Versus Stressed Collagen Matrices. J. Biol. Chem. 274: 918-923 [Abstract] [Full Text]
Gardner, H, Broberg, A, Pozzi, A, Laato, M, Heino, J (1999). Absence of integrin alpha1beta1 in the mouse causes loss of feedback regulation of collagen synthesis in normal and wounded dermis. J. Cell Sci. 112: 263-272 [Abstract]
Lambert, C. A., Lapiere, C. M., Nusgens, B. V. (1998). An Interleukin-1 Loop Is Induced in Human Skin Fibroblasts upon Stress Relaxation in a Three-dimensional Collagen Gel but Is Not Involved in the Up-regulation of Matrix Metalloproteinase 1. J. Biol. Chem. 273: 23143-23149 [Abstract] [Full Text]
Lorimier, S., Hornebeck, W., Godeau, G., Pellat, B., Gillery, P., Maquart, E., Laurent-Maquin, D. (1998). Morphometric Studies of Collagen and Fibrin Lattices Contracted by Human Gingival Fibroblasts; Comparison with Dermal Fibroblasts. JDR 77: 1717-1729 [Abstract]
Pozzi, A., Wary, K. K., Giancotti, F. G., Gardner, H. A. (1998). Integrin {alpha}1{beta}1 Mediates a Unique Collagen-dependent Proliferation Pathway In Vivo. JCB 142: 587-594 [Abstract] [Full Text]
Rezzonico, R., Burger, D., Dayer, J.-M. (1998). Direct Contact between T Lymphocytes and Human Dermal Fibroblasts or Synoviocytes Down-regulates Types I and III Collagen Production via Cell-associated Cytokines. J. Biol. Chem. 273: 18720-18728 [Abstract] [Full Text]
Hormuzdi, S. G., Penttinen, R., Jaenisch, R., Bornstein, P. (1998). A Gene-Targeting Approach Identifies a Function for the First Intron in Expression of the alpha 1(I) Collagen Gene. Mol. Cell. Biol. 18: 3368-3375 [Abstract] [Full Text]
Yanez-Mo, M., Alfranca, A., Cabanas, C., Marazuela, M., Tejedor, R., Angeles Ursa, M., Ashman, L. K., de Landazuri, M. O., Sanchez-Madrid, F. (1998). Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with {alpha}3{beta}1 Integrin Localized at Endothelial Lateral Junctions. JCB 141: 791-804 [Abstract] [Full Text]
Xu, J., Zutter, M. M., Santoro, S. A., Clark, R. A.F. (1998). A Three-dimensional Collagen Lattice Activates NF-{kappa}B in Human Fibroblasts: Role in Integrin {alpha}2 Gene Expression and Tissue Remodeling. JCB 140: 709-719 [Abstract] [Full Text]
Haas, T. L., Davis, S. J., Madri, J. A. (1998). Three-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells. J. Biol. Chem. 273: 3604-3610 [Abstract] [Full Text]
Stanton, H, Gavrilovic, J, Atkinson, S., d'Ortho, M., Yamada, K., Zardi, L, Murphy, G (1998). The activation of ProMMP-2 (gelatinase A) by HT1080 fibrosarcoma cells is promoted by culture on a fibronectin substrate and is concomitant with an increase in processing of MT1-MMP (MMP-14) to a 45 kDa form. J. Cell Sci. 111: 2789-2798 [Abstract]
Eckes, B, Dogic, D, Colucci-Guyon, E, Wang, N, Maniotis, A, Ingber, D, Merckling, A, Langa, F, Aumailley, M, Delouvee, A, Koteliansky, V, Babinet, C, Krieg, T (1998). Impaired mechanical stability, migration and contractile capacity in vimentin-deficient fibroblasts. J. Cell Sci. 111: 1897-1907 [Abstract]
Lee, K.-M., Tsai, K. Y., Wang, N., Ingber, D. E. (1998). Extracellular matrix and pulmonary hypertension: control of vascular smooth muscle cell contractility. Am. J. Physiol. Heart Circ. Physiol. 274: H76-H82 [Abstract] [Full Text]
Sudbeck, B. D., Pilcher, B. K., Welgus, H. G., Parks, W. C. (1997). Induction and Repression of Collagenase-1 by Keratinocytes Is Controlled by Distinct Components of Different Extracellular Matrix Compartments. J. Biol. Chem. 272: 22103-22110 [Abstract] [Full Text]
Pickering, J. G., Uniyal, S., Ford, C. M., Chau, T., Laurin, M. A., Chow, L. H., Ellis, C. G., Fish, J., Chan, B. M. C. (1997). Fibroblast Growth Factor-2 Potentiates Vascular Smooth Muscle Cell Migration to Platelet-Derived Growth Factor : Upregulation of {alpha}2�1 Integrin and Disassembly of Actin Filaments. Circ. Res. 80: 627-637 [Abstract] [Full Text]
Xu, J., Clark, R. A.F. (1997). A Three-dimensional Collagen Lattice Induces Protein Kinase C-{zeta} Activity: Role in {alpha}2 Integrin and Collagenase mRNA Expression. JCB 136: 473-483 [Abstract] [Full Text]
Palmieri, D., Camardella, L., Ulivi, V., Guasco, G., Manduca, P. (2000). Trimer Carboxyl Propeptide of Collagen I Produced by Mature Osteoblasts Is Chemotactic for Endothelial Cells. J. Biol. Chem. 275: 32658-32663 [Abstract] [Full Text]
Ellerbroek, S. M., Wu, Y. I., Overall, C. M., Stack, M. S. (2001). Functional Interplay between Type I Collagen and Cell Surface Matrix Metalloproteinase Activity. J. Biol. Chem. 276: 24833-24842 [Abstract] [Full Text]
Klekotka, P. A., Santoro, S. A., Zutter, M. M. (2001). alpha 2 Integrin Subunit Cytoplasmic Domain-dependent Cellular Migration Requires p38 MAPK. J. Biol. Chem. 276: 9503-9511 [Abstract] [Full Text]
Eble, J. A., Beermann, B., Hinz, H.-J., Schmidt-Hederich, A. (2001). alpha 2beta 1 Integrin Is Not Recognized by Rhodocytin but Is the Specific, High Affinity Target of Rhodocetin, an RGD-independent Disintegrin and Potent Inhibitor of Cell Adhesion to Collagen. J. Biol. Chem. 276: 12274-12284 [Abstract] [Full Text]
Kessler, D., Dethlefsen, S., Haase, I., Plomann, M., Hirche, F., Krieg, T., Eckes, B. (2001). Fibroblasts in Mechanically Stressed Collagen Lattices Assume a "Synthetic" Phenotype. J. Biol. Chem. 276: 36575-36585 [Abstract] [Full Text]
Klekotka, P. A., Santoro, S. A., Wang, H., Zutter, M. M. (2001). Specific Residues within the alpha 2 Integrin Subunit Cytoplasmic Domain Regulate Migration and Cell Cycle Progression via Distinct MAPK Pathways. J. Biol. Chem. 276: 32353-32361 [Abstract] [Full Text]