Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex

doi:10.1083/jcb.132.3.371

This Article

	Full Text (PDF, 1612K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wedaman, K. P.
	Articles by Scholey, J. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wedaman, K. P.
	Articles by Scholey, J. M.


Social Bookmarking

	What's this?

ARTICLES

Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex

KP Wedaman, DW Meyer, DJ Rashid, DG Cole and JM Scholey
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.

The heterotrimeric kinesin-II holoenzyme purified from sea urchin (Strongylocentrotus purpuratus) eggs is assembled from two heterodimerized kinesin-related motor subunits of known sequence, together with a third, previously uncharacterized 115-kD subunit, SpKAP115. Using monospecific anti-SpKAP115 antibodies we have accomplished the molecular cloning and sequencing of the SpKAP115 subunit. The deduced sequence predicts a globular 95-kD non-motor "accessory" polypeptide rich in alpha-helical segments that are generally not predicted to form coiled coils. Electron microscopy of individual rotary shadowed kinesin-II holoenzymes also suggests that SpKAP115 is globular, with a somewhat asymmetric morphology. Moreover, the SpKAP115 subunit lies at one end of the 51-nm-long kinesin-II complex, being separated from the two presumptive motor domains by a approximately 26-nm-long rod, in a manner similar to the light chains (KLCs) of kinesin itself. This indicates that SpKAP115 and the KLCs may have analogous functions, yet SpKAP115 does not display significant sequence similarity with the KLCs. The results show that kinesin and kinesin-II are assembled from highly divergent accessory polypeptides together with kinesin related motor subunits (KRPs) containing conserved motor domains linked to divergent tails. Despite the lack of sequence conservation outside the motor domains, there is striking conservation of the ultrastructure of the kinesin and kinesin-II holoenzymes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Carpenter, M. L., Cande, W. Z. (2009). Using Morpholinos for Gene Knockdown in Giardia intestinalis. Eukaryot Cell 8: 916-919 [Abstract] [Full Text]
Heinrich, B., Deshler, J. O. (2009). RNA localization to the Balbiani body in Xenopus oocytes is regulated by the energy state of the cell and is facilitated by kinesin II. RNA 15: 524-536 [Abstract] [Full Text]
Hirokawa, N., Noda, Y. (2008). Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics. Physiol. Rev. 88: 1089-1118 [Abstract] [Full Text]
Hoeng, J. C., Dawson, S. C., House, S. A., Sagolla, M. S., Pham, J. K., Mancuso, J. J., Lowe, J., Cande, W. Z. (2008). High-Resolution Crystal Structure and In Vivo Function of a Kinesin-2 Homologue in Giardia intestinalis. Mol. Biol. Cell 19: 3124-3137 [Abstract] [Full Text]
Scholey, J. M. (2008). Intraflagellar transport motors in cilia: moving along the cell's antenna. JCB 180: 23-29 [Abstract] [Full Text]
Imanishi, M., Endres, N. F., Gennerich, A., Vale, R. D. (2006). Autoinhibition regulates the motility of the C. elegans intraflagellar transport motor OSM-3. JCB 174: 931-937 [Abstract] [Full Text]
Pan, X., Ou, G., Civelekoglu-Scholey, G., Blacque, O. E., Endres, N. F., Tao, L., Mogilner, A., Leroux, M. R., Vale, R. D., Scholey, J. M. (2006). Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. JCB 174: 1035-1045 [Abstract] [Full Text]
Haraguchi, K., Hayashi, T., Jimbo, T., Yamamoto, T., Akiyama, T. (2006). Role of the Kinesin-2 Family Protein, KIF3, during Mitosis. J. Biol. Chem. 281: 4094-4099 [Abstract] [Full Text]
Miller, M. S., Esparza, J. M., Lippa, A. M., Lux, F. G. III, Cole, D. G., Dutcher, S. K. (2005). Mutant Kinesin-2 Motor Subunits Increase Chromosome Loss. Mol. Biol. Cell 16: 3810-3820 [Abstract] [Full Text]
Mueller, J., Perrone, C. A., Bower, R., Cole, D. G., Porter, M. E. (2005). The FLA3 KAP Subunit Is Required for Localization of Kinesin-2 to the Site of Flagellar Assembly and Processive Anterograde Intraflagellar Transport. Mol. Biol. Cell 16: 1341-1354 [Abstract] [Full Text]
Miki, H., Setou, M., Kaneshiro, K., Hirokawa, N. (2001). All kinesin superfamily protein, KIF, genes in mouse and human. Proc. Natl. Acad. Sci. USA 98: 7004-7011 [Abstract] [Full Text]
Ginkel, L. M., Wordeman, L. (2000). Expression and Partial Characterization of Kinesin-related Proteins in Differentiating and Adult Skeletal Muscle. Mol. Biol. Cell 11: 4143-4158 [Abstract] [Full Text]
Barrett, J. G., Manning, B. D., Snyder, M. (2000). The Kar3p Kinesin-related Protein Forms a Novel Heterodimeric Structure with Its Associated Protein Cik1p. Mol. Biol. Cell 11: 2373-2385 [Abstract] [Full Text]
Signor, D., Wedaman, K. P., Orozco, J. T., Dwyer, N. D., Bargmann, C. I., Rose, L. S., Scholey, J. M. (1999). Role of a Class Dhc1b Dynein in Retrograde Transport of Ift Motors and Ift Raft Particles along Cilia, but Not Dendrites, in Chemosensory Neurons of Living Caenorhabditis elegans. JCB 147: 519-530 [Abstract] [Full Text]
Gordon, D. M., Roof, D. M. (1999). The Kinesin-related Protein Kip1p of Saccharomyces cerevisiae Is Bipolar. J. Biol. Chem. 274: 28779-28786 [Abstract] [Full Text]
Marszalek, J. R., Ruiz-Lozano, P., Roberts, E., Chien, K. R., Goldstein, L. S.�B. (1999). Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II. Proc. Natl. Acad. Sci. USA 96: 5043-5048 [Abstract] [Full Text]
Pötter, E., Bergwitz, C., Brabant, G. (1999). The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues. Endocr. Rev. 20: 207-239 [Abstract] [Full Text]
Manning, B. D., Barrett, J. G., Wallace, J. A., Granok, H., Snyder, M. (1999). Differential Regulation of the Kar3p Kinesin-related Protein by Two Associated Proteins, Cik1p and Vik1p. JCB 144: 1219-1233 [Abstract] [Full Text]
Muresan, V., Lyass, A., Schnapp, B. J. (1999). The Kinesin Motor KIF3A Is a Component of the Presynaptic Ribbon in Vertebrate Photoreceptors. J. Neurosci. 19: 1027-1037 [Abstract] [Full Text]
Signor, D., Wedaman, K. P., Rose, L. S., Scholey, J. M. (1999). Two Heteromeric Kinesin Complexes in Chemosensory Neurons and Sensory Cilia of Caenorhabditis elegans. Mol. Biol. Cell 10: 345-360 [Abstract] [Full Text]
Le Bot, N., Antony, C., White, J., Karsenti, E., Vernos, I. (1998). Role of Xklp3, a Subunit of the Xenopus Kinesin II Heterotrimeric Complex, in Membrane Transport between the Endoplasmic Reticulum and the Golgi Apparatus. JCB 143: 1559-1573 [Abstract] [Full Text]
Tuma, M. C., Zill, A., Le Bot, N., Vernos, I., Gelfand, V. (1998). Heterotrimeric Kinesin II Is the Microtubule Motor Protein Responsible for Pigment Dispersion in Xenopus Melanophores. JCB 143: 1547-1558 [Abstract] [Full Text]
Smith, H. M. S., Raikhel, N. V. (1998). Nuclear Localization Signal Receptor Importin {alpha} Associates with the Cytoskeleton. Plant Cell 10: 1791-1800 [Abstract] [Full Text]
Cole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C., Rosenbaum, J. L. (1998). Chlamydomonas Kinesin-II-dependent Intraflagellar Transport (IFT): IFT Particles Contain Proteins Required for Ciliary Assembly in Caenorhabditis elegans Sensory Neurons. JCB 141: 993-1008 [Abstract] [Full Text]
Muresan, V., Abramson, T., Lyass, A., Winter, D., Porro, E., Hong, F., Chamberlin, N. L., Schnapp, B. J. (1998). KIF3C and KIF3A Form a Novel Neuronal Heteromeric Kinesin That Associates with Membrane Vesicles. Mol. Biol. Cell 9: 637-652 [Abstract] [Full Text]
Yang, Z., Goldstein, L. S.�B. (1998). Characterization of the KIF3C Neural Kinesin-like Motor from Mouse. Mol. Biol. Cell 9: 249-261 [Abstract] [Full Text]
Hirokawa, N. (1998). Kinesin and Dynein Superfamily Proteins and the Mechanism of Organelle Transport. Science 279: 519-526 [Abstract] [Full Text]
Morris, R. L., Scholey, J. M. (1997). Heterotrimeric Kinesin-II Is Required for the Assembly of Motile 9+2 Ciliary Axonemes on Sea Urchin Embryos. JCB 138: 1009-1022 [Abstract] [Full Text]
Shimizu, K., Kawabe, H., Minami, S., Honda, T., Takaishi, K., Shirataki, H., Takai, Y. (1996). SMAP, an Smg GDS-associating Protein Having Arm Repeats and Phosphorylated by Src Tyrosine Kinase. J. Biol. Chem. 271: 27013-27017 [Abstract] [Full Text]