Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast

doi:10.1083/jcb.133.1.75

This Article

	Full Text (PDF, 1301K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wells, W. A.
	Articles by Murray, A. W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wells, W. A.
	Articles by Murray, A. W.


Social Bookmarking

	What's this?

ARTICLES

Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast

WA Wells and AW Murray
Department of Physiology, University of California, San Francisco, 94143-0444, USA.

The spindle assembly checkpoint is the mechanism or set of mechanisms that prevents cells with defects in chromosome alignment or spindle assembly from passing through mitosis. We have investigated the effects of mini-chromosomes on this checkpoint in budding yeast by performing pedigree analysis. This method allowed us to observe the frequency and duration of cell cycle delays in individual cells. Short, centromeric linear mini-chromosomes, which have a low fidelity of segregation, cause frequent delays in mitosis. Their circular counterparts and longer linear mini-chromosomes, which segregate more efficiently, show a much lower frequency of mitotic delays, but these delays occur much more frequently in divisions where the mini-chromosome segregates to only one of the two daughter cells. Using a conditional centromere to increase the copy number of a circular mini-chromosome greatly increases the frequency of delayed divisions. In all cases the division delays are completely abolished by the mad mutants that inactivate the spindle assembly checkpoint, demonstrating that the Mad gene products are required to detect the subtle defects in chromosome behavior that have been observed to arrest higher eukaryotic cells in mitosis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Niikura, Y., Dixit, A., Scott, R., Perkins, G., Kitagawa, K. (2007). BUB1 mediation of caspase-independent mitotic death determines cell fate. JCB 178: 283-296 [Abstract] [Full Text]
Stoler, S., Rogers, K., Weitze, S., Morey, L., Fitzgerald-Hayes, M., Baker, R. E. (2007). Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc. Natl. Acad. Sci. USA 104: 10571-10576 [Abstract] [Full Text]
Palframan, W. J., Meehl, J. B., Jaspersen, S. L., Winey, M., Murray, A. W. (2006). Anaphase Inactivation of the Spindle Checkpoint. Science 313: 680-684 [Abstract] [Full Text]
Indjeian, V. B., Stern, B. M., Murray, A. W. (2005). The Centromeric Protein Sgo1 Is Required to Sense Lack of Tension on Mitotic Chromosomes. Science 307: 130-133 [Abstract] [Full Text]
Ubeda, M., Habener, J. F. (2003). CHOP Transcription Factor Phosphorylation by Casein Kinase 2 Inhibits Transcriptional Activation. J. Biol. Chem. 278: 40514-40520 [Abstract] [Full Text]
Biggins, S., Murray, A. W. (2001). The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the spindle checkpoint. Genes Dev. 15: 3118-3129 [Abstract] [Full Text]
Yang, M., Ma, H. (2001). Male Meiotic Spindle Lengths in Normal and Mutant Arabidopsis Cells. Plant Physiol. 126: 622-630 [Abstract] [Full Text]
Galgoczy, D. J., Toczyski, D. P. (2001). Checkpoint Adaptation Precedes Spontaneous and Damage-Induced Genomic Instability in Yeast. Mol. Cell. Biol. 21: 1710-1718 [Abstract] [Full Text]
Holloway, S. L. (2000). CHL1 is a nuclear protein with an essential ATP binding site that exhibits a size-dependent effect on chromosome segregation. Nucleic Acids Res 28: 3056-3064 [Abstract] [Full Text]
Rudner, A. D., Hardwick, K. G., Murray, A. W. (2000). Cdc28 Activates Exit from Mitosis in Budding Yeast. JCB 149: 1361-1376 [Abstract] [Full Text]
Lin, H., Choi, J. H., Hasek, J., DeLillo, N., Lou, W., Vancura, A. (2000). Phospholipase C Is Involved in Kinetochore Function in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 3597-3607 [Abstract] [Full Text]
DOBLES, M., SORGER, P.K. (2000). Mitotic Checkpoints, Genetic Instability, and Cancer. Cold Spring Harb Symp Quant Biol 65: 361-368 [Abstract]
Jones, M. H., Bachant, J. B., Castillo, A. R., Giddings, T. H. Jr., Winey, M. (1999). Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase. Mol. Biol. Cell 10: 2377-2391 [Abstract] [Full Text]
Hardwick, K. G., Li, R., Mistrot, C., Chen, R.-H., Dann, P., Rudner, A., Murray, A. W. (1999). Lesions in Many Different Spindle Components Activate the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae. Genetics 152: 509-518 [Abstract] [Full Text]
Hyland, K. M., Kingsbury, J., Koshland, D., Hieter, P. (1999). Ctf19p: A Novel Kinetochore Protein in Saccharomyces cerevisiae and a Potential Link between the Kinetochore and Mitotic Spindle. JCB 145: 15-28 [Abstract] [Full Text]
Clarke, A. S., Lowell, J. E., Jacobson, S. J., Pillus, L. (1999). Esa1p Is an Essential Histone Acetyltransferase Required for Cell Cycle Progression. Mol. Cell. Biol. 19: 2515-2526 [Abstract] [Full Text]
Sassoon, I., Severin, F. F., Andrews, P. D., Taba, M.-R., Kaplan, K. B., Ashford, A. J., Stark, M. J.R., Sorger, P. K., Hyman, A. A. (1999). Regulation of Saccharomyces cerevisiae kinetochores by the type 1�phosphatase Glc7p. Genes Dev. 13: 545-555 [Abstract] [Full Text]
Skibbens, R. V., Corson, L. B., Koshland, D., Hieter, P. (1999). Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery. Genes Dev. 13: 307-319 [Abstract] [Full Text]
Chan, G.K.T., Schaar, B.T., Yen, T.J. (1998). Characterization of the Kinetochore Binding Domain of CENP-E Reveals Interactions with the Kinetochore Proteins CENP-F and hBUBR1. JCB 143: 49-63 [Abstract] [Full Text]
Taylor, S. S., Ha, E., McKeon, F. (1998). The Human Homologue of Bub3 Is Required for Kinetochore Localization of Bub1 and a Mad3/Bub1-related Protein Kinase. JCB 142: 1-11 [Abstract] [Full Text]
Waters, J. C., Chen, R.-H., Murray, A. W., Salmon, E.D. (1998). Localization of Mad2 to Kinetochores Depends on Microtubule Attachment, Not Tension. JCB 141: 1181-1191 [Abstract] [Full Text]
Gorbsky, G. J., Chen, R.-H., Murray, A. W. (1998). Microinjection of Antibody to Mad2 Protein into Mammalian Cells in Mitosis Induces Premature Anaphase. JCB 141: 1193-1205 [Abstract] [Full Text]
Baker, R. E., Harris, K., Zhang, K. (1998). Mutations Synthetically Lethal with cep1 Target S. cerevisiae Kinetochore Components. Genetics 149: 73-85 [Abstract] [Full Text]
Tavormina, P. A., Burke, D. J. (1998). Cell Cycle Arrest in cdc20 Mutants of Saccharomyces cerevisiae Is Independent of Ndc10p and Kinetochore Function but Requires a Subset of Spindle Checkpoint Genes. Genetics 148: 1701-1713 [Abstract] [Full Text]
LeMaire-Adkins, R., Radke, K., Hunt, P. A. (1997). Lack of Checkpoint Control at the Metaphase/Anaphase Transition: A Mechanism of Meiotic Nondisjunction in Mammalian Females. JCB 139: 1611-1619 [Abstract] [Full Text]
Yao, X., Anderson, K. L., Cleveland, D. W. (1997). The Microtubule-dependent Motor Centromere-associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules. JCB 139: 435-447 [Abstract] [Full Text]
Straight, A. F., Marshall, W. F., Sedat, J. W., Murray, A. W. (1997). Mitosis in Living Budding Yeast: Anaphase A But No Metaphase Plate. Science 277: 574-578 [Abstract] [Full Text]
Saunders, W., Hornack, D., Lengyel, V., Deng, C. (1997). The Saccharomyces cerevisiae Kinesin-related Motor Kar3p Acts at Preanaphase Spindle Poles to Limit the Number and Length of Cytoplasmic Microtubules. JCB 137: 417-431 [Abstract] [Full Text]
Nicklas, R. B. (1997). How Cells Get the Right Chromosomes. Science 275: 632-637 [Abstract] [Full Text]
Li, X, Nicklas, R. (1997). Tension-sensitive kinetochore phosphorylation and the chromosome distribution checkpoint in praying mantid spermatocytes. J. Cell Sci. 110: 537-545 [Abstract]
Castano, I B, Brzoska, P M, Sadoff, B U, Chen, H, Christman, M F (1996). Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae.. Genes Dev. 10: 2564-2576 [Abstract]
Chen, R.-H., Waters, J. C., Salmon, E. D., Murray, A. W. (1996). Association of Spindle Assembly Checkpoint Component XMAD2 with Unattached Kinetochores. Science 274: 242-246 [Abstract] [Full Text]
Waters, J., Skibbens, R., Salmon, E. (1996). Oscillating mitotic newt lung cell kinetochores are, on average, under tension and rarely push. J. Cell Sci. 109: 2823-2831 [Abstract]