In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive

doi:10.1083/jcb.133.2.225

This Article

	Full Text (PDF, 6020K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Jordan, P.
	Articles by Carmo-Fonseca, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Jordan, P.
	Articles by Carmo-Fonseca, M.


Social Bookmarking

	What's this?

ARTICLES

In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive

P Jordan, M Mannervik, L Tora and M Carmo-Fonseca
Institute of Histology and Embryology, Faculty of Medicine, University of Lisbon, Portugal.

Here we show that the TATA-binding protein (TBP) is localized in the nucleoplasm and in the nucleolus of mammalian cells, consistent with its known involvement in transcription by RNA polymerase I, II, and III. In the nucleolus of actively growing cells, TBP colocalizes with upstream binding factor (UBF) and RNA polymerase I at the sites of rRNA transcription. During mitosis, when rRNA synthesis is down-regulated, TBP colocalizes with TBP-associated factors for RNA polymerase I (TAF(I)s), UBF, and RNA polymerase I on the chromosomal regions containing the rRNA genes. Treatment of cells with a low concentration of actinomycin D inhibits rRNA synthesis and causes a redistribution of the rRNA genes that become concentrated in clusters at the periphery of the nucleolus. A similar redistribution was observed for the major components of the rRNA transcription machinery (i.e., TBP, TAF(I)s, UBF, and RNA polymerase I), which still colocalized with each other. Furthermore, anti-TBP antibodies are shown to coimmunoprecipitate TBP and TAF(I)63 in extracts prepared from untreated and actinomycin D- treated cells. Collectively, the data indicate that in vivo TBP/promoter selectivity factor, UBF, and RNA polymerase I remain associated with both active and inactive rRNA genes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Hoppe, S., Bierhoff, H., Cado, I., Weber, A., Tiebe, M., Grummt, I., Voit, R. (2009). AMP-activated protein kinase adapts rRNA synthesis to cellular energy supply. Proc. Natl. Acad. Sci. USA 106: 17781-17786 [Abstract] [Full Text]
Goncalves, V., Matos, P., Jordan, P. (2009). Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet 18: 3696-3707 [Abstract] [Full Text]
Grob, A., Roussel, P., Wright, J. E., McStay, B., Hernandez-Verdun, D., Sirri, V. (2009). Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis. J. Cell Sci. 122: 489-498 [Abstract] [Full Text]
Messaoudi, L., Yang, Y.-G., Kinomura, A., Stavreva, D. A., Yan, G., Bortolin-Cavaille, M.-L., Arakawa, H., Buerstedde, J.-M., Hainaut, P., Cavaille, J., Takata, M., Van Dyck, E. (2007). Subcellular distribution of human RDM1 protein isoforms and their nucleolar accumulation in response to heat shock and proteotoxic stress. Nucleic Acids Res 35: 6571-6587 [Abstract] [Full Text]
Prieto, J.-L., McStay, B. (2007). Recruitment of factors linking transcription and processing of pre-rRNA to NOR chromatin is UBF-dependent and occurs independent of transcription in human cells. Genes Dev. 21: 2041-2054 [Abstract] [Full Text]
Wong, J. M.Y., Collins, K. (2006). Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita. Genes Dev. 20: 2848-2858 [Abstract] [Full Text]
Li, Y.-J., Macnaughton, T., Gao, L., Lai, M. M. C. (2006). RNA-Templated Replication of Hepatitis Delta Virus: Genomic and Antigenomic RNAs Associate with Different Nuclear Bodies.. J. Virol. 80: 6478-6486 [Abstract] [Full Text]
Sheng, Z., Liang, Y., Lin, C.-Y., Comai, L., Chirico, W. J. (2005). Direct Regulation of rRNA Transcription by Fibroblast Growth Factor 2. Mol. Cell. Biol. 25: 9419-9426 [Abstract] [Full Text]
Friedrich, J. K., Panov, K. I., Cabart, P., Russell, J., Zomerdijk, J. C. B. M. (2005). TBP-TAF Complex SL1 Directs RNA Polymerase I Pre-initiation Complex Formation and Stabilizes Upstream Binding Factor at the rDNA Promoter. J. Biol. Chem. 280: 29551-29558 [Abstract] [Full Text]
Shav-Tal, Y., Blechman, J., Darzacq, X., Montagna, C., Dye, B. T., Patton, J. G., Singer, R. H., Zipori, D. (2005). Dynamic Sorting of Nuclear Components into Distinct Nucleolar Caps during Transcriptional Inhibition. Mol. Biol. Cell 16: 2395-2413 [Abstract] [Full Text]
Chong, J. A., Moran, M. M., Teichmann, M., Kaczmarek, J. S., Roeder, R., Clapham, D. E. (2005). TATA-Binding Protein (TBP)-Like Factor (TLF) Is a Functional Regulator of Transcription: Reciprocal Regulation of the Neurofibromatosis Type 1 and c-fos Genes by TLF/TRF2 and TBP. Mol. Cell. Biol. 25: 2632-2643 [Abstract] [Full Text]
Chen, D., Dundr, M., Wang, C., Leung, A., Lamond, A., Misteli, T., Huang, S. (2005). Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins. JCB 168: 41-54 [Abstract] [Full Text]
Bubulya, P. A., Prasanth, K. V., Deerinck, T. J., Gerlich, D., Beaudouin, J., Ellisman, M. H., Ellenberg, J., Spector, D. L. (2004). Hypophosphorylated SR splicing factors transiently localize around active nucleolar organizing regions in telophase daughter nuclei. JCB 167: 51-63 [Abstract] [Full Text]
Kieffer-Kwon, P., Martianov, I., Davidson, I. (2004). Cell-specific Nucleolar Localization of TBP-related Factor 2. Mol. Biol. Cell 15: 4356-4368 [Abstract] [Full Text]
Leung, A. K. L., Gerlich, D., Miller, G., Lyon, C., Lam, Y. W., Lleres, D., Daigle, N., Zomerdijk, J., Ellenberg, J., Lamond, A. I. (2004). Quantitative kinetic analysis of nucleolar breakdown and reassembly during mitosis in live human cells. JCB 166: 787-800 [Abstract] [Full Text]
Baran, V., Pavlok, A., Bjerregaard, B., Wrenzycki, C., Hermann, D., Philimonenko, V. V., Lapathitis, G., Hozak, P., Niemann, H., Motlik, J. (2004). Immunolocalization of Upstream Binding Factor and Pocket Protein p130 During Final Stages of Bovine Oocyte Growth. Biol. Reprod. 70: 877-886 [Abstract] [Full Text]
Halkidou, K., Logan, I. R., Cook, S., Neal, D. E., Robson, C. N. (2004). Putative involvement of the histone acetyltransferase Tip60 in ribosomal gene transcription. Nucleic Acids Res 32: 1654-1665 [Abstract] [Full Text]
James, M. J., Zomerdijk, J. C. B. M. (2004). Phosphatidylinositol 3-Kinase and mTOR Signaling Pathways Regulate RNA Polymerase I Transcription in Response to IGF-1 and Nutrients. J. Biol. Chem. 279: 8911-8918 [Abstract] [Full Text]
Dynes, J. L., Xu, S., Bothner, S., Lahti, J. M., Hori, R. T. (2004). The Carboxyl-Terminus Directs TAFI48 to the Nucleus and Nucleolus and Associates with Multiple Nuclear Import Receptors. J Biochem 135: 429-438 [Abstract] [Full Text]
Bachand, F., Boisvert, F.-M., Cote, J., Richard, S., Autexier, C. (2002). The Product of the Survival of Motor Neuron (SMN) Gene is a Human Telomerase-associated Protein. Mol. Biol. Cell 13: 3192-3202 [Abstract] [Full Text]
O'Sullivan, A. C., Sullivan, G. J., McStay, B. (2002). UBF Binding In Vivo Is Not Restricted to Regulatory Sequences within the Vertebrate Ribosomal DNA Repeat. Mol. Cell. Biol. 22: 657-658 [Abstract] [Full Text]
Hernandez-Verdun, D., Roussel, P., Gebrane-Younes, J. (2002). Emerging concepts of nucleolar assembly. J. Cell Sci. 115: 2265-2270 [Abstract] [Full Text]
Chen, D., Hinkley, C. S., Henry, R. W., Huang, S. (2002). TBP Dynamics in Living Human Cells: Constitutive Association of TBP with Mitotic Chromosomes. Mol. Biol. Cell 13: 276-284 [Abstract] [Full Text]
Jones, K. W., Gorzynski, K., Hales, C. M., Fischer, U., Badbanchi, F., Terns, R. M., Terns, M. P. (2001). Direct Interaction of the Spinal Muscular Atrophy Disease Protein SMN with the Small Nucleolar RNA-associated Protein Fibrillarin. J. Biol. Chem. 276: 38645-38651 [Abstract] [Full Text]
Savino, T. M., Gebrane-Younes, J., De Mey, J., Sibarita, J.-B., Hernandez-Verdun, D. (2001). Nucleolar Assembly of the Rrna Processing Machinery in Living Cells. JCB 153: 1097-1110 [Abstract] [Full Text]
Fair, T., Hyttel, P., Lonergan, P., Boland, M. P. (2001). Immunolocalization of Nucleolar Proteins During Bovine Oocyte Growth, Meiotic Maturation, and Fertilization. Biol. Reprod. 64: 1516-1525 [Abstract] [Full Text]
Panov, K. I., Friedrich, J. K., Zomerdijk, J. C. B. M. (2001). A Step Subsequent to Preinitiation Complex Assembly at the Ribosomal RNA Gene Promoter Is Rate Limiting for Human RNA Polymerase I-Dependent Transcription. Mol. Cell. Biol. 21: 2641-2649 [Abstract] [Full Text]
Hyttel, P., Laurincik, J., Rosenkranz, Ch., Rath, D., Niemann, H., Ochs, R.L., Schellander, K. (2000). Nucleolar Proteins and Ultrastructure in Preimplantation Porcine Embryos Developed In Vivo. Biol. Reprod. 63: 1848-1856 [Abstract] [Full Text]
Szekely, A. M., Chen, Y.-H., Zhang, C., Oshima, J., Weissman, S. M. (2000). Werner protein recruits DNA polymerase delta to the nucleolus. Proc. Natl. Acad. Sci. USA 97: 11365-11370 [Abstract] [Full Text]
Dundr, M., Misteli, T., Olson, M. O.J. (2000). The Dynamics of Postmitotic Reassembly of the Nucleolus. JCB 150: 433-446 [Abstract] [Full Text]
Strezoska, Z., Pestov, D. G., Lau, L. F. (2000). Bop1 Is a Mouse WD40 Repeat Nucleolar Protein Involved in 28S and 5.8S rRNA Processing and 60S Ribosome Biogenesis. Mol. Cell. Biol. 20: 5516-5528 [Abstract] [Full Text]
Spencer, C. A., Kruhlak, M. J., Jenkins, H. L., Sun, X., Bazett-Jones, D. P. (2000). Mitotic Transcription Repression in Vivo in the Absence of Nucleosomal Chromatin Condensation. JCB 150: 13-26 [Abstract] [Full Text]
Szentirmay, M. N., Sawadogo, M. (2000). SURVEY AND SUMMARY: Spatial organization of RNA polymerase II transcription in the nucleus. Nucleic Acids Res 28: 2019-2025 [Abstract] [Full Text]
Fath, S., Milkereit, P., Podtelejnikov, A. V., Bischler, N., Schultz, P., Bier, M., Mann, M., Tschochner, H. (2000). Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in Rrna Synthesis and Processing. JCB 149: 575-590 [Abstract] [Full Text]
Laurincik, J., Thomsen, P.D., Hay-Schmidt, A., Avery, B., Greve, T., Ochs, R.L., Hyttel, P. (2000). Nucleolar Proteins and Nuclear Ultrastructure in Preimplantation Bovine Embryos Produced In Vitro. Biol. Reprod. 62: 1024-1032 [Abstract] [Full Text]
Sirri, V., Roussel, P., Hernandez-Verdun, D. (2000). In Vivo Release of Mitotic Silencing of Ribosomal Gene Transcription Does Not Give Rise to Precursor Ribosomal RNA Processing. JCB 148: 259-270 [Abstract] [Full Text]
Gall, J. G., Bellini, M., Wu, Z.'a., Murphy, C. (1999). Assembly of the Nuclear Transcription and Processing Machinery: Cajal Bodies (Coiled Bodies) and Transcriptosomes. Mol. Biol. Cell 10: 4385-4402 [Abstract] [Full Text]
Sirri, V, Roussel, P, Hernandez-Verdun, D (1999). The mitotically phosphorylated form of the transcription termination factor TTF-1 is associated with the repressed rDNA transcription machinery. J. Cell Sci. 112: 3259-3268 [Abstract]
Tseng, H, Biegel, J., Brown, R. (1999). Basonuclin is associated with the ribosomal RNA genes on human keratinocyte mitotic chromosomes. J. Cell Sci. 112: 3039-3047 [Abstract]
Piñol-Roma, S. (1999). Association of Nonribosomal Nucleolar Proteins in Ribonucleoprotein Complexes during Interphase and Mitosis. Mol. Biol. Cell 10: 77-90 [Abstract] [Full Text]
Klein, C., Cheutin, T., O'Donohue, M.-F., Rothblum, L., Kaplan, H., Beorchia, A., Lucas, L., Héliot, L., Ploton, D. (1998). The Three-dimensional Study of Chromosomes and Upstream Binding Factor-immunolabeled Nucleolar Organizer Regions Demonstrates Their Nonrandom Spatial Arrangement during Mitosis. Mol. Biol. Cell 9: 3147-3159 [Abstract] [Full Text]
Verheggen, C., Le Panse, S., Almouzni, G., Hernandez-Verdun, D. (1998). Presence of Pre-rRNAs before Activation of Polymerase I Transcription in the Building Process of Nucleoli during Early Development of Xenopus laevis. JCB 142: 1167-1180 [Abstract] [Full Text]
Dundr, M., Olson, M. O.J. (1998). Partially Processed pre-rRNA Is Preserved in Association with Processing Components in Nucleolus-derived Foci during Mitosis. Mol. Biol. Cell 9: 2407-2422 [Abstract] [Full Text]
Almeida, F., Saffrich, R., Ansorge, W., Carmo-Fonseca, M. (1998). Microinjection of Anti-coilin Antibodies Affects the Structure of Coiled Bodies. JCB 142: 899-912 [Abstract] [Full Text]
Hannan, K. M., Rothblum, L. I., Jefferson, L. S. (1998). Regulation of ribosomal DNA transcription by insulin. Am. J. Physiol. Cell Physiol. 275: C130-C138 [Abstract] [Full Text]
Gebrane-Younes, J, Fomproix, N, Hernandez-Verdun, D (1997). When rDNA transcription is arrested during mitosis, UBF is still associated with non-condensed rDNA. J. Cell Sci. 110: 2429-2440 [Abstract]
Bell, P, Mais, C, McStay, B, Scheer, U (1997). Association of the nucleolar transcription factor UBF with the transcriptionally inactive rRNA genes of pronuclei and early Xenopus embryos. J. Cell Sci. 110: 2053-2063 [Abstract]