Inhibition of I kappa B-alpha phosphorylation and degradation and subsequent NF-kappa B activation by glutathione peroxidase overexpression

doi:10.1083/jcb.133.5.1083

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Inhibition of I kappa B-alpha phosphorylation and degradation and subsequent NF-kappa B activation by glutathione peroxidase overexpression

C Kretz-Remy, P Mehlen, ME Mirault and AP Arrigo
Centre National de la Recherche Scientifique Unite Mixte de Recherche Universite Claude Bernard Lyon-I, France.

We report here that both kappa B-dependent transactivation of a reporter gene and NF-kappa B activation in response to tumor necrosis factor (TNF alpha) or H2O2 treatments are deficient in human T47D cell transfectants that overexpress seleno-glutathione peroxidase (GSHPx). These cells feature low reactive oxygen species (ROS) levels and decreased intracellular ROS burst in response to TNF alpha treatment. Decreased ROS levels and NF-kappa B activation were likely to result from GSHPx increment since these phenomena were no longer observed when GSHPx activity was reduced by selenium depletion. The cellular contents of the two NF-kappa B subunits (p65 and p50) and of the inhibitory subunit I kappa B-alpha were unaffected by GSHPx overexpression, suggesting that increased GSHPx activity interfered with the activation, but not the synthesis or stability, of Nf-kappa B. Nuclear translocation of NF-kappa B as well as I kappa B-alpha degradation were inhabited in GSHPx-overexpressing cells exposed to oxidative stress. Moreover, in control T47D cells exposed to TNF alpha, a time correlation was observed between elevated ROS levels and I kappa B- alpha degradation. We also show that, in growing T47D cells, GSHPx overexpression altered the isoform composition of I kappa B-alpha, leading to the accumulation of the more basic isoform of this protein. GSHPx overexpression also abolished the TNF alpha-mediated transient accumulation of the acidic and highly phosphorylated I kappa B-alpha isoform. These results suggest that intracellular ROS are key elements that regulate the phosphorylation of I kappa B-alpha, a phenomenon that precedes and controls the degradation of this protein, and then NF- kappa B activation.
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Au-Yeung, K. K.W., Woo, C. W.H., Sung, F. L., Yip, J. C.W., Siow, Y. L., O, K. (2004). Hyperhomocysteinemia Activates Nuclear Factor-{kappa}B in Endothelial Cells via Oxidative Stress. Circ. Res. 94: 28-36 [Abstract] [Full Text]
Maiorino, M., Scapin, M., Ursini, F., Biasolo, M., Bosello, V., Flohe, L. (2003). Distinct Promoters Determine Alternative Transcription of gpx-4 into Phospholipid-Hydroperoxide Glutathione Peroxidase Variants. J. Biol. Chem. 278: 34286-34290 [Abstract] [Full Text]
Parcellier, A., Schmitt, E., Gurbuxani, S., Seigneurin-Berny, D., Pance, A., Chantome, A., Plenchette, S., Khochbin, S., Solary, E., Garrido, C. (2003). HSP27 Is a Ubiquitin-Binding Protein Involved in I-{kappa}B{alpha} Proteasomal Degradation. Mol. Cell. Biol. 23: 5790-5802 [Abstract] [Full Text]
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Ouadrhiri, Y., Pilette, C., Monteiro, R. C., Vaerman, J.-P., Sibille, Y. (2002). Effect of IgA on Respiratory Burst and Cytokine Release by Human Alveolar Macrophages . Role of ERK1/2 Mitogen-Activated Protein Kinases and NF-kappa B. Am. J. Respir. Cell Mol. Bio. 26: 315-332 [Abstract] [Full Text]
Droge, W. (2002). Free Radicals in the Physiological Control of Cell Function. Physiol. Rev. 82: 47-95 [Abstract] [Full Text]
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Fan, J., Ye, R. D., Malik, A. B. (2001). Transcriptional mechanisms of acute lung injury. Am. J. Physiol. Lung Cell. Mol. Physiol. 281: L1037-L1050 [Abstract] [Full Text]
Li, S., Yan, T., Yang, J.-Q., Oberley, T. D., Oberley, L. W. (2000). The Role of Cellular Glutathione Peroxidase Redox Regulation in the Suppression of Tumor Cell Growth by Manganese Superoxide Dismutase. Cancer Res. 60: 3927-3939 [Abstract] [Full Text]
Jeon, K.-I., Jeong, J.-Y., Jue, D.-M. (2000). Thiol-Reactive Metal Compounds Inhibit NF-{kappa}B Activation by Blocking I{kappa}B Kinase. J. Immunol. 164: 5981-5989 [Abstract] [Full Text]
Kunsch, C., Medford, R. M. (1999). Oxidative Stress as a Regulator of Gene Expression in the Vasculature. Circ. Res. 85: 753-766 [Abstract] [Full Text]
Chu, F.-F., Esworthy, R. S., Lee, L., Wilczynski, S. (1999). Retinoic Acid Induces Gpx2 Gene Expression in MCF-7 Human Breast Cancer Cells. J. Nutr. 129: 1846-1854 [Abstract] [Full Text]
LI, N., KARIN, M. (1999). Is NF-{kappa}B the sensor of oxidative stress?. FASEB J. 13: 1137-1143 [Abstract] [Full Text]
Salvemini, F., Franze, A., Iervolino, A., Filosa, S., Salzano, S., Ursini, M. V. (1999). Enhanced Glutathione Levels and Oxidoresistance Mediated by Increased Glucose-6-phosphate Dehydrogenase Expression. J. Biol. Chem. 274: 2750-2757 [Abstract] [Full Text]
Li, J.-J., Oberley, L. W., Fan, M., Colburn, N. H. (1998). Inhibition of AP-1 and NF-{kappa}B by manganese-containing superoxide dismutase in human breast cancer cells. FASEB J. 12: 1713-1723 [Abstract] [Full Text]
Wen, W., Weiss, S. L., Sunde, R. A. (1998). UGA Codon Position Affects the Efficiency of Selenocysteine Incorporation into Glutathione Peroxidase-1. J. Biol. Chem. 273: 28533-28541 [Abstract] [Full Text]
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Lezoualc'h, F., Sagara, Y., Holsboer, F., Behl, C. (1998). High Constitutive NF-kappa B Activity Mediates Resistance to Oxidative Stress in Neuronal Cells. J. Neurosci. 18: 3224-3232 [Abstract] [Full Text]
Kang, S. W., Chae, H. Z., Seo, M. S., Kim, K., Baines, I. C., Rhee, S. G. (1998). Mammalian Peroxiredoxin Isoforms Can Reduce Hydrogen Peroxide Generated in Response to Growth Factors and Tumor Necrosis Factor-alpha. J. Biol. Chem. 273: 6297-6302 [Abstract] [Full Text]
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Lahdenpohja, N., Savinainen, K., Hurme, M. (1998). Pre-Exposure to Oxidative Stress Decreases the Nuclear Factor-{kappa}B-Dependent Transcription in T Lymphocytes. J. Immunol. 160: 1354-1358 [Abstract] [Full Text]
Jin, D.-Y., Chae, H. Z., Rhee, S. G., Jeang, K.-T. (1997). Regulatory Role for a Novel Human Thioredoxin Peroxidase in NF-kappa B Activation. J. Biol. Chem. 272: 30952-30961 [Abstract] [Full Text]
Benoliel, A., Kahn-Peries, B, Imbert, J, Verrando, P (1997). Insulin stimulates haptotactic migration of human epidermal keratinocytes through activation of NF-kappa B transcription factor. J. Cell Sci. 110: 2089-2097 [Abstract]
Daily, D., Vlamis-Gardikas, A., Offen, D., Mittelman, L., Melamed, E., Holmgren, A., Barzilai, A. (2001). Glutaredoxin Protects Cerebellar Granule Neurons from Dopamine-induced Apoptosis by Activating NF-kappa B via Ref-1. J. Biol. Chem. 276: 1335-1344 [Abstract] [Full Text]
Furling, D., Ghribi, O., Lahsaini, A., Mirault, M.-E., Massicotte, G. (2000). Impairment of synaptic transmission by transient hypoxia in hippocampal slices: Improved recovery in glutathione peroxidase transgenic mice. Proc. Natl. Acad. Sci. USA 97: 4351-4356 [Abstract] [Full Text]