Rho-stimulated contractility drives the formation of stress fibers and focal adhesions

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Rho-stimulated contractility drives the formation of stress fibers and focal adhesions

M Chrzanowska-Wodnicka and K Burridge
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA.

Activated rhoA, a ras-related GTP-binding protein, stimulates the appearance of stress fibers, focal adhesions, and tyrosine phosphorylation in quiescent cells (Ridley, A.J., and A. Hall, 1992. Cell. 70:389-399). The pathway by which rho triggers these events has not been elucidated. Many of the agents that activate rho (e.g., vasopressin, endothelin, lysophosphatidic acid) stimulate the contractility of smooth muscle and other cells. We have investigated whether rho's induction of stress fibers, focal adhesions, and tyrosine phosphorylation is the result of its stimulation of contractility. We demonstrate that stimulation of fibroblasts with lysophosphatidic acid, which activates rho, induces myosin light chain phosphorylation. This precedes the formation of stress fibers and focal adhesions and is accompanied by increased contractility. Inhibition of contractility by several different mechanisms leads to inhibition of rho-induced stress fibers, focal adhesions, and tyrosine phosphorylation. In addition, when contractility is inhibited, integrins disperse from focal adhesions as stress fibers and focal adhesions disassemble. Conversely, upon stimulation of contractility, diffusely distributed integrins are aggregated into focal adhesions. These results suggest that activated rho stimulates contractility, driving the formation of stress fibers and focal adhesions and elevating tyrosine phosphorylation. A model is proposed to account for how contractility could promote these events.
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Hirata, H., Tatsumi, H., Sokabe, M. (2008). Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner. J. Cell Sci. 121: 2795-2804 [Abstract] [Full Text]
Wylie, S. R., Chantler, P. D. (2008). Myosin IIC: A Third Molecular Motor Driving Neuronal Dynamics. Mol. Biol. Cell 19: 3956-3968 [Abstract] [Full Text]
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Leon, C., Eckly, A., Hechler, B., Aleil, B., Freund, M., Ravanat, C., Jourdain, M., Nonne, C., Weber, J., Tiedt, R., Gratacap, M.-P., Severin, S., Cazenave, J.-P., Lanza, F., Skoda, R., Gachet, C. (2007). Megakaryocyte-restricted MYH9 inactivation dramatically affects hemostasis while preserving platelet aggregation and secretion. Blood 110: 3183-3191 [Abstract] [Full Text]
Pellegrin, S., Mellor, H. (2007). Actin stress fibres. J. Cell Sci. 120: 3491-3499 [Abstract] [Full Text]
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Gupton, S. L., Eisenmann, K., Alberts, A. S., Waterman-Storer, C. M. (2007). mDia2 regulates actin and focal adhesion dynamics and organization in the lamella for efficient epithelial cell migration. J. Cell Sci. 120: 3475-3487 [Abstract] [Full Text]
Peacock, J. G., Miller, A. L., Bradley, W. D., Rodriguez, O. C., Webb, D. J., Koleske, A. J. (2007). The Abl-related Gene Tyrosine Kinase Acts through p190RhoGAP to Inhibit Actomyosin Contractility and Regulate Focal Adhesion Dynamics upon Adhesion to Fibronectin. Mol. Biol. Cell 18: 3860-3872 [Abstract] [Full Text]
Gopinath, S. D., Narumiya, S., Dhawan, J. (2007). The RhoA effector mDiaphanous regulates MyoD expression and cell cycle progression via SRF-dependent and SRF-independent pathways. J. Cell Sci. 120: 3086-3098 [Abstract] [Full Text]
Feral, C. C., Zijlstra, A., Tkachenko, E., Prager, G., Gardel, M. L., Slepak, M., Ginsberg, M. H. (2007). CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling. JCB 178: 701-711 [Abstract] [Full Text]
Dulyaninova, N. G., House, R. P., Betapudi, V., Bresnick, A. R. (2007). Myosin-IIA Heavy-Chain Phosphorylation Regulates the Motility of MDA-MB-231 Carcinoma Cells. Mol. Biol. Cell 18: 3144-3155 [Abstract] [Full Text]
Zhang, S. J., Truskey, G. A., Kraus, W. E. (2007). Effect of cyclic stretch on beta1D-integrin expression and activation of FAK and RhoA. Am. J. Physiol. Cell Physiol. 292: C2057-C2069 [Abstract] [Full Text]
Zhao, X.-H., Laschinger, C., Arora, P., Szaszi, K., Kapus, A., McCulloch, C. A. (2007). Force activates smooth muscle {alpha}-actin promoter activity through the Rho signaling pathway. J. Cell Sci. 120: 1801-1809 [Abstract] [Full Text]
Guo, Y., Satpathy, M., Wilson, G., Srinivas, S. P. (2007). Benzalkonium Chloride Induces Dephosphorylation of Myosin Light Chain in Cultured Corneal Epithelial Cells. IOVS 48: 2001-2008 [Abstract] [Full Text]
Sanka, K., Maddala, R., Epstein, D. L., Rao, P. V. (2007). Influence of Actin Cytoskeletal Integrity on Matrix Metalloproteinase-2 Activation in Cultured Human Trabecular Meshwork Cells. IOVS 48: 2105-2114 [Abstract] [Full Text]
Malek, A. M., Xu, C., Kim, E. S., Alper, S. L. (2007). Hypertonicity triggers RhoA-dependent assembly of myosin-containing striated polygonal actin networks in endothelial cells. Am. J. Physiol. Cell Physiol. 292: C1645-C1659 [Abstract] [Full Text]
Taubenberger, A., Cisneros, D. A., Friedrichs, J., Puech, P.-H., Muller, D. J., Franz, C. M. (2007). Revealing Early Steps of {alpha}2beta1 Integrin-mediated Adhesion to Collagen Type I by Using Single-Cell Force Spectroscopy. Mol. Biol. Cell 18: 1634-1644 [Abstract] [Full Text]
Chien, S. (2007). Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell. Am. J. Physiol. Heart Circ. Physiol. 292: H1209-H1224 [Abstract] [Full Text]
Vicente-Manzanares, M., Zareno, J., Whitmore, L., Choi, C. K., Horwitz, A. F. (2007). Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells. JCB 176: 573-580 [Abstract] [Full Text]
Schober, M., Raghavan, S., Nikolova, M., Polak, L., Pasolli, H. A., Beggs, H. E., Reichardt, L. F., Fuchs, E. (2007). Focal adhesion kinase modulates tension signaling to control actin and focal adhesion dynamics. JCB 176: 667-680 [Abstract] [Full Text]
Williams, M. J., Habayeb, M. S., Hultmark, D. (2007). Reciprocal regulation of Rac1 and Rho1 in Drosophila circulating immune surveillance cells. J. Cell Sci. 120: 502-511 [Abstract] [Full Text]
Watanabe, T., Hosoya, H., Yonemura, S. (2007). Regulation of Myosin II Dynamics by Phosphorylation and Dephosphorylation of Its Light Chain in Epithelial Cells. Mol. Biol. Cell 18: 605-616 [Abstract] [Full Text]
Hu, K., Ji, L., Applegate, K. T., Danuser, G., Waterman-Storer, C. M. (2007). Differential Transmission of Actin Motion Within Focal Adhesions. Science 315: 111-115 [Abstract] [Full Text]
Sandquist, J. C., Swenson, K. I., DeMali, K. A., Burridge, K., Means, A. R. (2006). Rho Kinase Differentially Regulates Phosphorylation of Nonmuscle Myosin II Isoforms A and B during Cell Rounding and Migration. J. Biol. Chem. 281: 35873-35883 [Abstract] [Full Text]
Scott, J. A., Yap, A. S. (2006). Cinderella no longer: {alpha}-catenin steps out of cadherin's shadow. J. Cell Sci. 119: 4599-4605 [Abstract] [Full Text]
Meyer-ter-Vehn, T., Sieprath, S., Katzenberger, B., Gebhardt, S., Grehn, F., Schlunck, G. (2006). Contractility as a Prerequisite for TGF-{beta}-Induced Myofibroblast Transdifferentiation in Human Tenon Fibroblasts. IOVS 47: 4895-4904 [Abstract] [Full Text]
Risinger, G. M. Jr., Hunt, T. S., Updike, D. L., Bullen, E. C., Howard, E. W. (2006). Matrix Metalloproteinase-2 Expression by Vascular Smooth Muscle Cells Is Mediated by Both Stimulatory and Inhibitory Signals in Response to Growth Factors. J. Biol. Chem. 281: 25915-25925 [Abstract] [Full Text]
Liu, Z., Kostenko, E. V., Mahon, G. M., Olabisi, O. O., Whitehead, I. P. (2006). Transformation by the Rho-specific Guanine Nucleotide Exchange Factor Dbs Requires ROCK I-mediated Phosphorylation of Myosin Light Chain. J. Biol. Chem. 281: 16043-16051 [Abstract] [Full Text]
Connell, L. E., Helfman, D. M. (2006). Myosin light chain kinase plays a role in the regulation of epithelial cell survival. J. Cell Sci. 119: 2269-2281 [Abstract] [Full Text]
Nayal, A., Webb, D. J., Brown, C. M., Schaefer, E. M., Vicente-Manzanares, M., Horwitz, A. R. (2006). Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics. JCB 173: 587-589 [Abstract] [Full Text]
Williams, M. J., Wiklund, M.-L., Wikman, S., Hultmark, D. (2006). Rac1 signalling in the Drosophila larval cellular immune response. J. Cell Sci. 119: 2015-2024 [Abstract] [Full Text]
Zhang, Z.-G., Bothe, I., Hirche, F., Zweers, M., Gullberg, D., Pfitzer, G., Krieg, T., Eckes, B., Aumailley, M. (2006). Interactions of primary fibroblasts and keratinocytes with extracellular matrix proteins: contribution of {alpha}2{beta}1 integrin.. J. Cell Sci. 119: 1886-1895 [Abstract] [Full Text]
Eves, R., Webb, B. A., Zhou, S., Mak, A. S. (2006). Caldesmon is an integral component of podosomes in smooth muscle cells. J. Cell Sci. 119: 1691-1702 [Abstract] [Full Text]
Leslie, M. (2006). Integrin signal transduction. JCB 173: 150a-150a [Full Text]
Matthews, B. D., Overby, D. R., Mannix, R., Ingber, D. E. (2006). Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels. J. Cell Sci. 119: 508-518 [Abstract] [Full Text]
Woo, S., Gomez, T. M. (2006). Rac1 and RhoA Promote Neurite Outgrowth through Formation and Stabilization of Growth Cone Point Contacts. J. Neurosci. 26: 1418-1428 [Abstract] [Full Text]
Minambres, R., Guasch, R. M., Perez-Arago, A., Guerri, C. (2006). The RhoA/ROCK-I/MLC pathway is involved in the ethanol-induced apoptosis by anoikis in astrocytes. J. Cell Sci. 119: 271-282 [Abstract] [Full Text]
Mehta, D., Malik, A. B. (2006). Signaling Mechanisms Regulating Endothelial Permeability. Physiol. Rev. 86: 279-367 [Abstract] [Full Text]
Surks, H. K., Riddick, N., Ohtani, K.-i. (2005). M-RIP Targets Myosin Phosphatase to Stress Fibers to Regulate Myosin Light Chain Phosphorylation in Vascular Smooth Muscle Cells. J. Biol. Chem. 280: 42543-42551 [Abstract] [Full Text]
Discher, D. E., Janmey, P., Wang, Y.-l. (2005). Tissue Cells Feel and Respond to the Stiffness of Their Substrate. Science 310: 1139-1143 [Abstract] [Full Text]
Hildebrand, J. D. (2005). Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network. J. Cell Sci. 118: 5191-5203 [Abstract] [Full Text]
Zhang, M., Rao, P. V. (2005). Blebbistatin, a Novel Inhibitor of Myosin II ATPase Activity, Increases Aqueous Humor Outflow Facility in Perfused Enucleated Porcine Eyes. IOVS 46: 4130-4138 [Abstract] [Full Text]
Kaunas, R., Nguyen, P., Usami, S., Chien, S. (2005). From The Cover: Cooperative effects of Rho and mechanical stretch on stress fiber organization. Proc. Natl. Acad. Sci. USA 102: 15895-15900 [Abstract] [Full Text]
Fox, D. T., Homem, C. C. F., Myster, S. H., Wang, F., Bain, E. E., Peifer, M. (2005). Rho1 regulates Drosophila adherens junctions independently of p120ctn. Development 132: 4819-4831 [Abstract] [Full Text]
de Rooij, J., Kerstens, A., Danuser, G., Schwartz, M. A., Waterman-Storer, C. M. (2005). Integrin-dependent actomyosin contraction regulates epithelial cell scattering. JCB 171: 153-164 [Abstract] [Full Text]
Shewan, A. M., Maddugoda, M., Kraemer, A., Stehbens, S. J., Verma, S., Kovacs, E. M., Yap, A. S. (2005). Myosin 2 Is a Key Rho Kinase Target Necessary for the Local Concentration of E-Cadherin at Cell-Cell Contacts. Mol. Biol. Cell 16: 4531-4542 [Abstract] [Full Text]
Zaidel-Bar, R., Kam, Z., Geiger, B. (2005). Polarized downregulation of the paxillin-p130CAS-Rac1 pathway induced by shear flow. J. Cell Sci. 118: 3997-4007 [Abstract] [Full Text]
Gallant, N. D., Michael, K. E., Garcia, A. J. (2005). Cell Adhesion Strengthening: Contributions of Adhesive Area, Integrin Binding, and Focal Adhesion Assembly. Mol. Biol. Cell 16: 4329-4340 [Abstract] [Full Text]
Shemesh, T., Geiger, B., Bershadsky, A. D., Kozlov, M. M. (2005). Focal adhesions as mechanosensors: A physical mechanism. Proc. Natl. Acad. Sci. USA 102: 12383-12388 [Abstract] [Full Text]
Wang, R., Clark, R. A. F., Mosher, D. F., Ren, X.-D. (2005). Fibronectin's Central Cell-binding Domain Supports Focal Adhesion Formation and Rho Signal Transduction. J. Biol. Chem. 280: 28803-28810 [Abstract] [Full Text]
Simoes, R. L., Fierro, I. M. (2005). Involvement of the Rho-Kinase/Myosin Light Chain Kinase Pathway on Human Monocyte Chemotaxis Induced by ATL-1, an Aspirin-Triggered Lipoxin A4 Synthetic Analog. J. Immunol. 175: 1843-1850 [Abstract] [Full Text]
Fazal, F., Gu, L., Ihnatovych, I., Han, Y., Hu, W., Antic, N., Carreira, F., Blomquist, J. F., Hope, T. J., Ucker, D. S., de Lanerolle, P. (2005). Inhibiting Myosin Light Chain Kinase Induces Apoptosis In Vitro and In Vivo. Mol. Cell. Biol. 25: 6259-6266 [Abstract] [Full Text]
Barfod, E. T., Moore, A. L., Melnick, R. F., Lidofsky, S. D. (2005). Src Regulates Distinct Pathways for Cell Volume Control through Vav and Phospholipase C{gamma}. J. Biol. Chem. 280: 25548-25557 [Abstract] [Full Text]
Song, J., Deng, P.-F., Stinnett, S. S., Epstein, D. L., Rao, P. V. (2005). Effects of Cholesterol-Lowering Statins on the Aqueous Humor Outflow Pathway. IOVS 46: 2424-2432 [Abstract] [Full Text]
Ciano-Oliveira, C. D., Lodyga, M., Fan, L., Szaszi, K., Hosoya, H., Rotstein, O. D., Kapus, A. (2005). Is myosin light-chain phosphorylation a regulatory signal for the osmotic activation of the Na+-K+-2Cl- cotransporter?. Am. J. Physiol. Cell Physiol. 289: C68-C81 [Abstract] [Full Text]
Ohanian, V., Gatfield, K., Ohanian, J. (2005). Role of the Actin Cytoskeleton in G-Protein-Coupled Receptor Activation of PYK2 and Paxillin in Vascular Smooth Muscle. Hypertension 46: 93-99 [Abstract] [Full Text]
Laroche, G., Rochdi, M. D., Laporte, S. A., Parent, J.-L. (2005). Involvement of Actin in Agonist-induced Endocytosis of the G Protein-coupled Receptor for Thromboxane A2: OVERCOMING OF ACTIN DISRUPTION BY ARRESTIN-3 BUT NOT ARRESTIN-2. J. Biol. Chem. 280: 23215-23224 [Abstract] [Full Text]
Pullikuth, A., McKinnon, E., Schaeffer, H.-J., Catling, A. D. (2005). The MEK1 Scaffolding Protein MP1 Regulates Cell Spreading by Integrating PAK1 and Rho Signals. Mol. Cell. Biol. 25: 5119-5133 [Abstract] [Full Text]
Geho, D. H., Bandle, R. W., Clair, T., Liotta, L. A. (2005). Physiological Mechanisms of Tumor-Cell Invasion and Migration. Physiology 20: 194-200 [Abstract] [Full Text]
Formigli, L., Meacci, E., Sassoli, C., Chellini, F., Giannini, R., Quercioli, F., Tiribilli, B., Squecco, R., Bruni, P., Francini, F., Zecchi-Orlandini, S. (2005). Sphingosine 1-phosphate induces cytoskeletal reorganization in C2C12 myoblasts: physiological relevance for stress fibres in the modulation of ion current through stretch-activated channels. J. Cell Sci. 118: 1161-1171 [Abstract] [Full Text]
Gupton, S. L., Anderson, K. L., Kole, T. P., Fischer, R. S., Ponti, A., Hitchcock-DeGregori, S. E., Danuser, G., Fowler, V. M., Wirtz, D., Hanein, D., Waterman-Storer, C. M. (2005). Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin. JCB 168: 619-631 [Abstract] [Full Text]
Sun, B., Nishihira, J., Yoshiki, T., Kondo, M., Sato, Y., Sasaki, F., Todo, S. (2005). Macrophage Migration Inhibitory Factor Promotes Tumor Invasion and Metastasis via the Rho-Dependent Pathway. Clin. Cancer Res. 11: 1050-1058 [Abstract] [Full Text]