Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope

doi:10.1083/jcb.134.2.315

This Article

Full Text (PDF, 2582K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Ma, Y.

Articles by Schnell, D. J.

Search for Related Content

PubMed

PubMed Citation

Articles by Ma, Y.

Articles by Schnell, D. J.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope

Y Ma, A Kouranov, SE LaSala and DJ Schnell
Department of Biological Sciences, Rutgers, State University of New Jersey, Newark 07102, USA.

The interactions of precursor proteins with components of the chloroplast envelope were investigated during the early stages of protein import using a chemical cross-linking strategy. In the absence of energy, two components of the outer envelope import machinery, IAP86 and IAP75, cross-linked to the transit sequence of the precursor to the small subunit of ribulose-1, 5-bisphosphate carboxylase (pS) in a precursor binding assay. In the presence of concentrations of ATP or GTP that support maximal precursor binding to the envelope, cross- linking to the transit sequence occurred predominantly with IAP75 and a previously unidentified 21-kD polypeptide of the inner membrane, indicating that the transit sequence had inserted across the outer membrane. Cross-linking of envelope components to sequences in the mature portion of a second precursor, preferredoxin, was detected in the presence of ATP or GTP, suggesting that sequences distant from the transit sequence were brought into the vicinity of the outer membrane under these conditions. IAP75 and a third import component, IAP34, were coimmunoprecipitated with IAP86 antibodies from solubilized envelope membranes, indicating that these three proteins form a stable complex in the outer membrane. On the basis of these observations, we propose that IAP86 and IAP75 act as components of a multisubunit complex to mediate energy-independent recognition of the transit sequence and subsequent nucleoside triphosphate-induced insertion of the transit sequence across the outer membrane.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Lee, J., Wang, F., Schnell, D. J. (2009). Toc Receptor Dimerization Participates in the Initiation of Membrane Translocation during Protein Import into Chloroplasts. J. Biol. Chem. 284: 31130-31141 [Abstract] [Full Text]
Jouhet, J., Gray, J. C. (2009). Interaction of Actin and the Chloroplast Protein Import Apparatus. J. Biol. Chem. 284: 19132-19141 [Abstract] [Full Text]
Kikuchi, S., Oishi, M., Hirabayashi, Y., Lee, D. W., Hwang, I., Nakai, M. (2009). A 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope Membrane. Plant Cell 21: 1781-1797 [Abstract] [Full Text]
Wang, F., Agne, B., Kessler, F., Schnell, D. J. (2008). The role of GTP binding and hydrolysis at the atToc159 preprotein receptor during protein import into chloroplasts. JCB 183: 87-99 [Abstract] [Full Text]
Reinbothe, C., Pollmann, S., Phetsarath-Faure, P., Quigley, F., Weisbeek, P., Reinbothe, S. (2008). A Pentapeptide Motif Related to a Pigment Binding Site in the Major Light-Harvesting Protein of Photosystem II, LHCII, Governs Substrate-Dependent Plastid Import of NADPH:Protochlorophyllide Oxidoreductase A. Plant Physiol. 148: 694-703 [Abstract] [Full Text]
Inoue, H., Akita, M. (2008). Three Sets of Translocation Intermediates Are Formed during the Early Stage of Protein Import into Chloroplasts. J. Biol. Chem. 283: 7491-7502 [Abstract] [Full Text]
Schemenewitz, A., Pollmann, S., Reinbothe, C., Reinbothe, S. (2007). A substrate-independent, 14:3:3 protein-mediated plastid import pathway of NADPH:protochlorophyllide oxidoreductase A. Proc. Natl. Acad. Sci. USA 104: 8538-8543 [Abstract] [Full Text]
Reinbothe, C., Bartsch, S., Eggink, L. L., Hoober, J. K., Brusslan, J., Andrade-Paz, R., Monnet, J., Reinbothe, S. (2006). A role for chlorophyllide a oxygenase in the regulated import and stabilization of light-harvesting chlorophyll a/b proteins. Proc. Natl. Acad. Sci. USA 103: 4777-4782 [Abstract] [Full Text]
Kikuchi, S., Hirohashi, T., Nakai, M. (2006). Characterization of the Preprotein Translocon at the Outer Envelope Membrane of Chloroplasts by Blue Native PAGE. Plant Cell Physiol 47: 363-371 [Abstract] [Full Text]
Bedard, J., Jarvis, P. (2005). Recognition and envelope translocation of chloroplast preproteins. J Exp Bot 56: 2287-2320 [Abstract] [Full Text]
Surana, N. K., Grass, S., Hardy, G. G., Li, H., Thanassi, D. G., Geme, J. W. St. III (2004). Evidence for conservation of architecture and physical properties of Omp85-like proteins throughout evolution. Proc. Natl. Acad. Sci. USA 101: 14497-14502 [Abstract] [Full Text]
Nada, A., Soll, J. (2004). Inner envelope protein 32 is imported into chloroplasts by a novel pathway. J. Cell Sci. 117: 3975-3982 [Abstract] [Full Text]
Tu, S.-L., Chen, L.-J., Smith, M. D., Su, Y.-s., Schnell, D. J., Li, H.-m. (2004). Import Pathways of Chloroplast Interior Proteins and the Outer-Membrane Protein OEP14 Converge at Toc75. Plant Cell 16: 2078-2088 [Abstract] [Full Text]
Kubis, S., Patel, R., Combe, J., Bedard, J., Kovacheva, S., Lilley, K., Biehl, A., Leister, D., Rios, G., Koncz, C., Jarvis, P. (2004). Functional Specialization amongst the Arabidopsis Toc159 Family of Chloroplast Protein Import Receptors. Plant Cell 16: 2059-2077 [Abstract] [Full Text]
Reinbothe, S., Quigley, F., Gray, J., Schemenewitz, A., Reinbothe, C. (2004). Identification of plastid envelope proteins required for import of protochlorophyllide oxidoreductase A into the chloroplast of barley. Proc. Natl. Acad. Sci. USA 101: 2197-2202 [Abstract] [Full Text]
Reinbothe, S., Quigley, F., Springer, A., Schemenewitz, A., Reinbothe, C. (2004). The outer plastid envelope protein Oep16: Role as precursor translocase in import of protochlorophyllide oxidoreductase A. Proc. Natl. Acad. Sci. USA 101: 2203-2208 [Abstract] [Full Text]
Inaba, T., Li, M., Alvarez-Huerta, M., Kessler, F., Schnell, D. J. (2003). atTic110 Functions as a Scaffold for Coordinating the Stromal Events of Protein Import into Chloroplasts. J. Biol. Chem. 278: 38617-38627 [Abstract] [Full Text]
Kubis, S., Baldwin, A., Patel, R., Razzaq, A., Dupree, P., Lilley, K., Kurth, J., Leister, D., Jarvis, P. (2003). The Arabidopsis ppi1 Mutant Is Specifically Defective in the Expression, Chloroplast Import, and Accumulation of Photosynthetic Proteins. Plant Cell 15: 1859-1871 [Abstract] [Full Text]
Schleiff, E., Soll, J., Kuchler, M., Kuhlbrandt, W., Harrer, R. (2003). Characterization of the translocon of the outer envelope of chloroplasts. JCB 160: 541-551 [Abstract] [Full Text]
Bauer, J., Hiltbrunner, A., Weibel, P., Vidi, P.-A., Alvarez-Huerta, M., Smith, M. D., Schnell, D. J., Kessler, F. (2002). Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane. JCB 159: 845-854 [Abstract] [Full Text]
Miras, S., Salvi, D., Ferro, M., Grunwald, D., Garin, J., Joyard, J., Rolland, N. (2002). Non-canonical Transit Peptide for Import into the Chloroplast. J. Biol. Chem. 277: 47770-47778 [Abstract] [Full Text]
Sun, C.-W., Chen, L.-J., Lin, L.-C., Li, H.-m. (2001). Leaf-Specific Upregulation of Chloroplast Translocon Genes by a CCT Motif-Containing Protein, CIA 2. Plant Cell 13: 2053-2061 [Abstract] [Full Text]
Yu, T.-S., Li, H.-m. (2001). Chloroplast Protein Translocon Components atToc159 and atToc33 Are Not Essential for Chloroplast Biogenesis in Guard Cells and Root Cells. Plant Physiol. 127: 90-96 [Abstract] [Full Text]
Hiltbrunner, A., Bauer, J., Vidi, P.-A., Infanger, S., Weibel, P., Hohwy, M., Kessler, F. (2001). Targeting of an abundant cytosolic form of the protein import receptor at Toc159 to the outer chloroplast membrane. JCB 154: 309-316 [Abstract] [Full Text]
Jackson-Constan, D., Keegstra, K. (2001). Arabidopsis Genes Encoding Components of the Chloroplastic Protein Import Apparatus. Plant Physiol. 125: 1567-1576 [Abstract] [Full Text]
Hirohashi, T., Hase, T., Nakai, M. (2001). Maize Non-Photosynthetic Ferredoxin Precursor Is Mis-Sorted to the Intermembrane Space of Chloroplasts in the Presence of Light. Plant Physiol. 125: 2154-2163 [Abstract] [Full Text]
Row, P. E., Gray, J. C. (2001). Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts. J Exp Bot 52: 47-56 [Abstract] [Full Text]
Row, P. E., Gray, J. C. (2001). The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates. J Exp Bot 52: 57-66 [Abstract] [Full Text]
McFadden, G. I. (2001). Chloroplast Origin and Integration. Plant Physiol. 125: 50-53 [Full Text]
Rensink, W. A., Schnell, D. J., Weisbeek, P. J. (2000). The Transit Sequence of Ferredoxin Contains Different Domains for Translocation across the Outer and Inner Membrane of the Chloroplast Envelope. J. Biol. Chem. 275: 10265-10271 [Abstract] [Full Text]
May, T., Soll, J. (2000). 14-3-3 Proteins Form a Guidance Complex with Chloroplast Precursor Proteins in Plants. Plant Cell 12: 53-64 [Abstract] [Full Text]
Keegstra, K., Cline, K. (1999). Protein Import and Routing Systems of Chloroplasts. Plant Cell 11: 557-570 [Full Text]
Bolter, B., Soll, J., Schulz, A., Hinnah, S., Wagner, R. (1998). Origin of a chloroplast protein importer. Proc. Natl. Acad. Sci. USA 95: 15831-15836 [Abstract] [Full Text]
Kouranov, A., Schnell, D. J. (1997). Analysis of the Interactions of Preproteins with the Import Machinery over the Course of Protein Import into Chloroplasts. JCB 139: 1677-1685 [Abstract] [Full Text]
Lawrence, S. D., Kindle, K. L. (1997). Alterations in the Chlamydomonas Plastocyanin Transit Peptide Have Distinct Effects on in Vitro Import and in Vivo Protein Accumulation. J. Biol. Chem. 272: 20357-20363 [Abstract] [Full Text]
Rapaport, D., Neupert, W., Lill, R. (1997). Mitochondrial Protein Import. Tom40 PLAYS A MAJOR ROLE IN TARGETING AND TRANSLOCATION OF PREPROTEINS BY FORMING A SPECIFIC BINDING SITE FOR THE PRESEQUENCE. J. Biol. Chem. 272: 18725-18731 [Abstract] [Full Text]
Li, H.-m., Chen, L.-J. (1997). A Novel Chloroplastic Outer Membrane-targeting Signal That Functions at Both Termini of Passenger Polypeptides. J. Biol. Chem. 272: 10968-10974 [Abstract] [Full Text]
Akita, M., Nielsen, E., Keegstra, K. (1997). Identification of Protein Transport Complexes in the Chloroplastic Envelope Membranes via Chemical Cross-Linking. JCB 136: 983-994 [Abstract] [Full Text]
Kouranov, A., Schnell, D. J. (1996). Protein Translocation at the Envelope and Thylakoid Membranes of Chloroplasts. J. Biol. Chem. 271: 31009-31012 [Full Text]
Reinbothe, S., Mache, R., Reinbothe, C. (2000). A second, substrate-dependent site of protein import into chloroplasts. Proc. Natl. Acad. Sci. USA 97: 9795-9800 [Abstract] [Full Text]
Chen, X., Smith, M. D., Fitzpatrick, L., Schnell, D. J. (2002). In Vivo Analysis of the Role of atTic20 in Protein Import into Chloroplasts. Plant Cell 14: 641-654 [Abstract] [Full Text]