ARIA/HRG regulates AChR epsilon subunit gene expression at the neuromuscular synapse via activation of phosphatidylinositol 3-kinase and Ras/MAPK pathway

doi:10.1083/jcb.134.2.465

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ARIA/HRG regulates AChR epsilon subunit gene expression at the neuromuscular synapse via activation of phosphatidylinositol 3-kinase and Ras/MAPK pathway

MG Tansey, GC Chu and JP Merlie
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St.Louis, Missouri 63110-8103, USA.

AChR-inducing activity (ARIA)/heregulin, a ligand for erbB receptor tyrosine kinases (RTKs), is likely to be one nerve-supplied signal that induces expression of acetylcholine receptor (AChR) genes at the developing neuromuscular junction. Since some RTKs act through Ras and phosphatidylinositol 3-kinase (PI3K), we investigated the role of these pathways in ARIA signaling. Expression of activated Ras or Raf mimicked ARIA-induction of AChR epsilon subunit genes in muscle cells; whereas dominant negative Ras or Raf blocked the effect of ARIA. ARIA rapidly activated erk1 and erk2 and inhibition of both erks also abolished the effect of ARIA. ARIA stimulated association of PI3K with erbB3, expression of an activated PI3K led to ARIA-independent AChR epsilon subunit expression, and inhibition of PI3K abolished the action of ARIA. Thus, synaptic induction of AChR genes requires activation of both Ras/MAPK and PI3K signal transduction pathways.
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Lacazette, E., Le Calvez, S., Gajendran, N., Brenner, H. R. (2003). A novel pathway for MuSK to induce key genes in neuromuscular synapse formation. JCB 161: 727-736 [Abstract] [Full Text]
Lebrasseur, N. K., Cote, G. M., Miller, T. A., Fielding, R. A., Sawyer, D. B. (2003). Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle. Am. J. Physiol. Cell Physiol. 284: C1149-C1155 [Abstract] [Full Text]
Ma, L., Huang, Y. Z., Pitcher, G. M., Valtschanoff, J. G., Ma, Y. H., Feng, L. Y., Lu, B., Xiong, W. C., Salter, M. W., Weinberg, R. J., Mei, L. (2003). Ligand-Dependent Recruitment of the ErbB4 Signaling Complex into Neuronal Lipid Rafts. J. Neurosci. 23: 3164-3175 [Abstract] [Full Text]
Huang, Y. Z., Zang, M., Xiong, W. C., Luo, Z., Mei, L. (2003). Erbin Suppresses the MAP Kinase Pathway. J. Biol. Chem. 278: 1108-1114 [Abstract] [Full Text]
Andrechek, E. R., Hardy, W. R., Girgis-Gabardo, A. A., Perry, R. L. S., Butler, R., Graham, F. L., Kahn, R. C., Rudnicki, M. A., Muller, W. J. (2002). ErbB2 Is Required for Muscle Spindle and Myoblast Cell Survival. Mol. Cell. Biol. 22: 4714-4722 [Abstract] [Full Text]
Smith, C. L., Mittaud, P., Prescott, E. D., Fuhrer, C., Burden, S. J. (2001). Src, Fyn, and Yes Are Not Required for Neuromuscular Synapse Formation But Are Necessary for Stabilization of Agrin-Induced Clusters of Acetylcholine Receptors. J. Neurosci. 21: 3151-3160 [Abstract] [Full Text]
Ozaki, M. (2001). Neuregulins and the Shaping of Synapses. Neuroscientist 7: 146-154 [Abstract]
Hertig, C. M., Kubalak, S. W., Wang, Y., Chien, K. R. (1999). Synergistic Roles of Neuregulin-1 and Insulin-like Growth Factor-I in Activation of the Phosphatidylinositol 3-Kinase Pathway and Cardiac Chamber Morphogenesis. J. Biol. Chem. 274: 37362-37369 [Abstract] [Full Text]
Tanowitz, M., Si, J., Yu, D.-H., Feng, G.-S., Mei, L. (1999). Regulation of Neuregulin-Mediated Acetylcholine Receptor Synthesis by Protein Tyrosine Phosphatase SHP2. J. Neurosci. 19: 9426-9435 [Abstract] [Full Text]
Si, J., Wang, Q., Mei, L. (1999). Essential Roles of c-JUN and c-JUN N-Terminal Kinase (JNK) in Neuregulin-Increased Expression of the Acetylcholine Receptor epsilon -Subunit. J. Neurosci. 19: 8498-8508 [Abstract] [Full Text]
Khurana, T. S., Rosmarin, A. G., Shang, J., Krag, T. O.�B., Das, S., Gammeltoft, S. (1999). Activation of Utrophin Promoter by Heregulin via the ets-related Transcription Factor Complex GA-binding Protein alpha /beta. Mol. Biol. Cell 10: 2075-2086 [Abstract] [Full Text]
Wang, D., Huang, H-J. S., Kazlauskas, A., Cavenee, W. K. (1999). Induction of Vascular Endothelial Growth Factor Expression in Endothelial Cells by Platelet-derived Growth Factor through the Activation of Phosphatidylinositol 3-Kinase. Cancer Res. 59: 1464-1472 [Abstract] [Full Text]
Gramolini, A. O., Angus, L. M., Schaeffer, L., Burton, E. A., Tinsley, J. M., Davies, K. E., Changeux, J.-P., Jasmin, B. J. (1999). Induction of utrophin gene expression by heregulin in skeletal muscle cells: Role of the N-box motif and GA binding protein. Proc. Natl. Acad. Sci. USA 96: 3223-3227 [Abstract] [Full Text]
Chausovsky, A., Tsarfaty, I., Kam, Z., Yarden, Y., Geiger, B., Bershadsky, A. D. (1998). Morphogenetic Effects of Neuregulin (Neu Differentiation Factor) in Cultured Epithelial Cells. Mol. Biol. Cell 9: 3195-3209 [Abstract] [Full Text]
Fromm, L., Burden, S. J. (1998). Synapse-specific and neuregulin-induced transcription require an Ets site that binds GABPalpha /GABPbeta. Genes Dev. 12: 3074-3083 [Abstract] [Full Text]
Meier, T., Masciulli, F., Moore, C., Schoumacher, F., Eppenberger, U., Denzer, A. J., Jones, G., Brenner, H. R. (1998). Agrin Can Mediate Acetylcholine Receptor Gene Expression in Muscle by Aggregation of Muscle-derived Neuregulins. JCB 141: 715-726 [Abstract] [Full Text]
Engel, A. G., Ohno, K., Wang, H.-L., Milone, M., Sine, S. M. (1998). REVIEW {blacksquare} : Molecular Basis of Congenital Myasthenic Syndromes: Mutations in the Acetylcholine Receptor. Neuroscientist 4: 185-194 [Abstract]
Sapru, M. K., Florance, S. K., Kirk, C., Goldman, D. (1998). Identification of a neuregulin and protein-tyrosine phosphatase response element in the nicotinic acetylcholine receptor varepsilon �subunit gene: Regulatory role of an Ets�transcription�factor. Proc. Natl. Acad. Sci. USA 95: 1289-1294 [Abstract] [Full Text]
Burden, S. J. (1998). The formation of neuromuscular�synapses. Genes Dev. 12: 133-148 [Full Text]
Colledge, M., Froehner, S. C. (1997). Tyrosine Phosphorylation of Nicotinic Acetylcholine Receptor Mediates Grb2 Binding. J. Neurosci. 17: 5038-5045 [Abstract] [Full Text]
Smith, M. A., Fanger, G. R., O'Connor, L. T., Bridle, P., Maue, R. A. (1997). Selective Regulation of Agrin mRNA Induction and Alternative Splicing in PC12 Cells by Ras-dependent Actions of Nerve Growth Factor. J. Biol. Chem. 272: 15675-15681 [Abstract] [Full Text]
Si, J., Miller, D. S., Mei, L. (1997). Identification of an Element Required for Acetylcholine Receptor-inducing Activity (ARIA)-induced Expression of the Acetylcholine Receptor epsilon Subunit Gene. J. Biol. Chem. 272: 10367-10371 [Abstract] [Full Text]
Huang, Y. Z., Wang, Q., Xiong, W. C., Mei, L. (2001). Erbin Is a Protein Concentrated at Postsynaptic Membranes That Interacts with PSD-95. J. Biol. Chem. 276: 19318-19326 [Abstract] [Full Text]
Tang, H., Sun, Z., Goldman, D. (2001). CaM Kinase II-dependent Suppression of Nicotinic Acetylcholine Receptor delta -Subunit Promoter Activity. J. Biol. Chem. 276: 26057-26065 [Abstract] [Full Text]
Li, Q., Loeb, J. A. (2001). Neuregulin-Heparan-sulfate Proteoglycan Interactions Produce Sustained erbB Receptor Activation Required for the Induction of Acetylcholine Receptors in Muscle. J. Biol. Chem. 276: 38068-38075 [Abstract] [Full Text]