The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae

doi:10.1083/jcb.134.6.1349

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The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae

M Gotta, T Laroche, A Formenton, L Maillet, H Scherthan and SM Gasser
Swiss Institute for Experimental Cancer Research, Epalinges/Lausanne, Switzerland.

We have developed a novel technique for combined immunofluorescence/in situ hybridization on fixed budding yeast cells that maintains the three-dimensional structure of the nucleus as monitored by focal sections of cells labeled with fluorescent probes and by staining with a nuclear pore antibody. Within the resolution of these immunodetection techniques, we show that proteins encoded by the SIR3, SIR4, and RAP1 genes colocalize in a statistically significant manner with Y' telomere- associated DNA sequences. In wild-type cells the Y' in situ hybridization signals can be resolved by light microscopy into fewer than ten foci per diploid nucleus. This suggests that telomeres are clustered in vegetatively growing cells, and that proteins essential for telomeric silencing are concentrated at their sites of action, i.e., at telomeres and/or subtelomeric regions. As observed for Rap1, the Sir4p staining is diffuse in a sir3- strain, and similarly, Sir3p staining is no longer punctate in a sir4- strain, although the derivatized Y' probe continues to label discrete sites in these strains. Nonetheless, the Y' FISH is altered in a qualitative manner in sir3 and sir4 mutant strains, consistent with the previously reported phenotypes of shortened telomeric repeats and loss of telomeric silencing.
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Oakes, M. L., Johzuka, K., Vu, L., Eliason, K., Nomura, M. (2006). Expression of rRNA Genes and Nucleolus Formation at Ectopic Chromosomal Sites in the Yeast Saccharomyces cerevisiae.. Mol. Cell. Biol. 26: 6223-6238 [Abstract] [Full Text]
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Casolari, J. M., Brown, C. R., Drubin, D. A., Rando, O. J., Silver, P. A. (2005). Developmentally induced changes in transcriptional program alter spatial organization across chromosomes. Genes Dev. 19: 1188-1198 [Abstract] [Full Text]
Menon, B. B., Sarma, N. J., Pasula, S., Deminoff, S. J., Willis, K. A., Barbara, K. E., Andrews, B., Santangelo, G. M. (2005). Reverse recruitment: The Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc. Natl. Acad. Sci. USA 102: 5749-5754 [Abstract] [Full Text]
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Van Dyke, M. W., Nelson, L. D., Weilbaecher, R. G., Mehta, D. V. (2004). Stm1p, a G4 Quadruplex and Purine Motif Triplex Nucleic Acid-binding Protein, Interacts with Ribosomes and Subtelomeric Y' DNA in Saccharomyces cerevisiae. J. Biol. Chem. 279: 24323-24333 [Abstract] [Full Text]
Orlandi, I., Bettiga, M., Alberghina, L., Vai, M. (2004). Transcriptional Profiling of ubp10 Null Mutant Reveals Altered Subtelomeric Gene Expression and Insurgence of Oxidative Stress Response. J. Biol. Chem. 279: 6414-6425 [Abstract] [Full Text]
GASSER, S.M., HEDIGER, F., TADDEI, A., NEUMANN, F.R., GARTENBERG, M.R. (2004). The Function of Telomere Clustering in Yeast: The Circe Effect. Cold Spring Harb Symp Quant Biol 69: 327-338 [Abstract]
Papacs, L. A., Sun, Y., Anderson, E. L., Sun, J., Holmes, S. G. (2004). REP3-Mediated Silencing in Saccharomyces cerevisiae. Genetics 166: 79-87 [Abstract] [Full Text]
Andrulis, E. D., Zappulla, D. C., Alexieva-Botcheva, K., Evangelista, C., Sternglanz, R. (2004). One-Hybrid Screens at the Saccharomyces cerevisiae HMR Locus Identify Novel Transcriptional Silencing Factors. Genetics 166: 631-635 [Abstract] [Full Text]
Smolikov, S., Krauskopf, A. (2003). The Rap1p-Telomere Complex Does Not Determine the Replicative Capacity of Telomerase-Deficient Yeast. Mol. Cell. Biol. 23: 8729-8739 [Abstract] [Full Text]
Lorenz, A., Fuchs, J., Burger, R., Loidl, J. (2003). Chromosome Pairing Does Not Contribute to Nuclear Architecture in Vegetative Yeast Cells. Eukaryot Cell 2: 856-866 [Abstract] [Full Text]
Livingstone-Zatchej, M., Marcionelli, R., Moller, K., de Pril, R., Thoma, F. (2003). Repair of UV Lesions in Silenced Chromatin Provides in Vivo Evidence for a Compact Chromatin Structure. J. Biol. Chem. 278: 37471-37479 [Abstract] [Full Text]
Trelles-Sticken, E., Loidl, J., Scherthan, H. (2003). Increased ploidy and KAR3 and SIR3 disruption alter the dynamics of meiotic chromosomes and telomeres. J. Cell Sci. 116: 2431-2442 [Abstract] [Full Text]
Lebrun, E., Fourel, G., Defossez, P.-A., Gilson, E. (2003). A Methyltransferase Targeting Assay Reveals Silencer-Telomere Interactions in Budding Yeast. Mol. Cell. Biol. 23: 1498-1508 [Abstract] [Full Text]
Smith, C.D., Smith, D.L., DeRisi, J.L., Blackburn, E.H. (2003). Telomeric Protein Distributions and Remodeling Through the Cell Cycle in Saccharomyces cerevisiae. Mol. Biol. Cell 14: 556-570 [Abstract] [Full Text]
Miyoshi, T., Sadaie, M., Kanoh, J., Ishikawa, F. (2003). Telomeric DNA Ends Are Essential for the Localization of Ku at Telomeres in Fission Yeast. J. Biol. Chem. 278: 1924-1931 [Abstract] [Full Text]
Andrulis, E. D., Zappulla, D. C., Ansari, A., Perrod, S., Laiosa, C. V., Gartenberg, M. R., Sternglanz, R. (2002). Esc1, a Nuclear Periphery Protein Required for Sir4-Based Plasmid Anchoring and Partitioning. Mol. Cell. Biol. 22: 8292-8301 [Abstract] [Full Text]
Choe, W., Budd, M., Imamura, O., Hoopes, L., Campbell, J. L. (2002). Dynamic Localization of an Okazaki Fragment Processing Protein Suggests a Novel Role in Telomere Replication. Mol. Cell. Biol. 22: 4202-4217 [Abstract] [Full Text]
Ostashevsky, J. (2002). A Polymer Model for Large-scale Chromatin Organization in Lower Eukaryotes. Mol. Biol. Cell 13: 2157-2169 [Abstract] [Full Text]
Huang, Y. (2002). Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Nucleic Acids Res 30: 1465-1482 [Abstract] [Full Text]
Fuchs, J., Lorenz, A., Loidl, J. (2002). Chromosome associations in budding yeast caused by integrated tandemly repeated transgenes. J. Cell Sci. 115: 1213-1220 [Abstract] [Full Text]
Dekker, J., Rippe, K., Dekker, M., Kleckner, N. (2002). Capturing Chromosome Conformation. Science 295: 1306-1311 [Abstract] [Full Text]
Lorenz, A., Fuchs, J., Trelles-Sticken, E., Scherthan, H., Loidl, J. (2002). Spatial organisation and behaviour of the parental chromosome sets in the nuclei of Saccharomyces cerevisiae x S. paradoxus hybrids. J. Cell Sci. 115: 3829-3835 [Abstract] [Full Text]
Sharp, J. A., Franco, A. A., Osley, M. A., Kaufman, P. D. (2002). Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev. 16: 85-100 [Abstract] [Full Text]
Lewis, L. K., Karthikeyan, G., Westmoreland, J. W., Resnick, M. A. (2002). Differential Suppression of DNA Repair Deficiencies of Yeast rad50, mre11 and xrs2 Mutants by EXO1 and TLC1 (the RNA Component of Telomerase). Genetics 160: 49-62 [Abstract] [Full Text]
Heun, P., Laroche, T., Shimada, K., Furrer, P., Gasser, S. M. (2001). Chromosome Dynamics in the Yeast Interphase Nucleus. Science 294: 2181-2186 [Abstract] [Full Text]
Moretti, P., Shore, D. (2001). Multiple Interactions in Sir Protein Recruitment by Rap1p at Silencers and Telomeres in Yeast. Mol. Cell. Biol. 21: 8082-8094 [Abstract] [Full Text]
Mangahas, J. L., Alexander, M. K., Sandell, L. L., Zakian, V. A. (2001). Repair of Chromosome Ends after Telomere Loss in Saccharomyces. Mol. Biol. Cell 12: 4078-4089 [Abstract] [Full Text]
Melo, J. A., Cohen, J., Toczyski, D. P. (2001). Two checkpoint complexes are independently recruited to sites of DNA damage in vivo. Genes Dev. 15: 2809-2821 [Abstract] [Full Text]
Craven, R. J., Petes, T. D. (2001). The Saccharomyces cerevisiae Suppressor of Choline Sensitivity (SCS2) Gene Is a Multicopy Suppressor of mec1 Telomeric Silencing Defects. Genetics 158: 145-154 [Abstract] [Full Text]
Heun, P., Laroche, T., Raghuraman, M.K., Gasser, S. M. (2001). The Positioning and Dynamics of Origins of Replication in the Budding Yeast Nucleus. JCB 152: 385-400 [Abstract] [Full Text]
Scherthan, H., Jerratsch, M., Li, B., Smith, S., Hultén, M., Lock, T., de Lange, T. (2000). Mammalian Meiotic Telomeres: Protein Composition and Redistribution in Relation to Nuclear Pores. Mol. Biol. Cell 11: 4189-4203 [Abstract] [Full Text]
de Bruin, D., Kantrow, S. M., Liberatore, R. A., Zakian, V. A. (2000). Telomere Folding Is Required for the Stable Maintenance of Telomere Position Effects in Yeast. Mol. Cell. Biol. 20: 7991-8000 [Abstract] [Full Text]
Migliaccio, M., Amacker, M., Just, T., Reichenbach, P., Valmori, D., Cerottini, J.-C., Romero, P., Nabholz, M. (2000). Ectopic Human Telomerase Catalytic Subunit Expression Maintains Telomere Length But Is Not Sufficient for CD8+ T Lymphocyte Immortalization. J. Immunol. 165: 4978-4984 [Abstract] [Full Text]
Trelles-Sticken, E., Dresser, M. E., Scherthan, H. (2000). Meiotic Telomere Protein Ndj1p Is Required for Meiosis-Specific Telomere Distribution, Bouquet Formation and Efficient Homologue Pairing. JCB 151: 95-106 [Abstract] [Full Text]
San-Segundo, P. A., Roeder, G. S. (2000). Role for the Silencing Protein Dot1 in Meiotic Checkpoint Control. Mol. Biol. Cell 11: 3601-3615 [Abstract] [Full Text]
Shinohara, M., Gasior, S. L., Bishop, D. K., Shinohara, A. (2000). Tid1/Rdh54 promotes colocalization of Rad51 and Dmc1 during meiotic recombination. Proc. Natl. Acad. Sci. USA 97: 10814-10819 [Abstract] [Full Text]
Enomoto, S., Johnston, S. D., Berman, J. (2000). Identification of a Novel Allele of SIR3 Defective in the Maintenance, but Not the Establishment, of Silencing in Saccharomyces cerevisiae. Genetics 155: 523-538 [Abstract] [Full Text]
Cockell, M. M., Perrod, S., Gasser, S. M. (2000). Analysis of Sir2p Domains Required for rDNA and Telomeric Silencing in Saccharomyces cerevisiae. Genetics 154: 1069-1083 [Abstract] [Full Text]
Park, Y., Lustig, A. J. (2000). Telomere Structure Regulates the Heritability of Repressed Subtelomeric Chromatin in Saccharomyces cerevisiae. Genetics 154: 587-598 [Abstract] [Full Text]
Jin, Q., Fuchs, J, Loidl, J (2000). Centromere clustering is a major determinant of yeast interphase nuclear organization. J. Cell Sci. 113: 1903-1912 [Abstract]
Frei, C., Gasser, S. M. (2000). The yeast Sgs1p helicase acts upstream of Rad53p in the DNA replication checkpoint and colocalizes with Rad53p in S-phase-specific foci. Genes Dev. 14: 81-96 [Abstract] [Full Text]
Weipoltshammer, K., Schofer, C., Almeder, M., Philimonenko, V. V., Frei, K., Wachtler, F., Hozak, P. (1999). Intranuclear Anchoring of Repetitive DNA Sequences: Centromeres, Telomeres, and Ribosomal DNA. JCB 147: 1409-1418 [Abstract] [Full Text]
Kahana, A., Gottschling, D. E. (1999). DOT4 Links Silencing and Cell Growth in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 6608-6620 [Abstract] [Full Text]
Xu, E. Y., Kim, S., Rivier, D. H. (1999). SAS4 and SAS5 Are Locus-Specific Regulators of Silencing in Saccharomyces cerevisiae. Genetics 153: 25-33 [Abstract] [Full Text]
Pasero, P., Duncker, B. P., Schwob, E., Gasser, S. M. (1999). A role for the Cdc7 kinase regulatory subunit Dbf4p in the formation of initiation-competent origins of replication. Genes Dev. 13: 2159-2176 [Abstract] [Full Text]
Matsuura, A., Naito, T., Ishikawa, F. (1999). Genetic Control of Telomere Integrity in Schizosaccharomyces pombe: rad3+ and tel1+ Are Parts of Two Regulatory Networks Independent of the Downstream Protein Kinases chk1+ and cds1+. Genetics 152: 1501-1512 [Abstract] [Full Text]
Rine, J. (1999). On the mechanism of silencing in Escherichia coli. Proc. Natl. Acad. Sci. USA 96: 8309-8311 [Full Text]
Kim, S.-K., Wang, J. C. (1999). Gene silencing via protein-mediated subcellular localization of DNA. Proc. Natl. Acad. Sci. USA 96: 8557-8561 [Abstract] [Full Text]
Burgess, S. M., Kleckner, N. (1999). Collisions between yeast chromosomal loci in vivo are governed by three layers of organization. Genes Dev. 13: 1871-1883 [Abstract] [Full Text]
Croft, J. A., Bridger, J. M., Boyle, S., Perry, P., Teague, P., Bickmore, W. A. (1999). Differences in the Localization and Morphology of Chromosomes in the Human Nucleus. JCB 145: 1119-1131 [Abstract] [Full Text]
Trelles-Sticken, E, Loidl, J, Scherthan, H (1999). Bouquet formation in budding yeast: initiation of recombination is not required for meiotic telomere clustering. J. Cell Sci. 112: 651-658 [Abstract]
Park, Y., Hanish, J., Lustig, A. J. (1998). Sir3p Domains Involved in the Initiation of Telomeric Silencing in Saccharomyces cerevisiae. Genetics 150: 977-986 [Abstract] [Full Text]
Gotta, M., Palladino, F., Gasser, S. M. (1998). Functional Characterization of the N Terminus of Sir3p. Mol. Cell. Biol. 18: 6110-6120 [Abstract] [Full Text]
Lew, J. E., Enomoto, S., Berman, J. (1998). Telomere Length Regulation and Telomeric Chromatin Require the Nonsense-Mediated mRNA Decay Pathway. Mol. Cell. Biol. 18: 6121-6130 [Abstract] [Full Text]
Stavenhagen, J. B., Zakian, V. A. (1998). Yeast telomeres exert a position effect on recombination between internal tracts of yeast telomeric DNA. Genes Dev. 12: 3044-3058 [Abstract] [Full Text]
Bourns, B. D., Alexander, M. K., Smith, A. M., Zakian, V. A. (1998). Sir Proteins, Rif Proteins, and Cdc13p Bind Saccharomyces Telomeres In Vivo. Mol. Cell. Biol. 18: 5600-5608 [Abstract] [Full Text]
Huang, D. W., Fanti, L., Pak, D. T.S., Botchan, M. R., Pimpinelli, S., Kellum, R. (1998). Distinct Cytoplasmic and Nuclear Fractions of Drosophila Heterochromatin Protein 1: Their Phosphorylation Levels and Associations with Origin Recognition Complex Proteins. JCB 142: 307-318 [Abstract] [Full Text]
Gasior, S. L., Wong, A. K., Kora, Y., Shinohara, A., Bishop, D. K. (1998). Rad52 associates with RPA and functions with Rad55 and Rad57 to assemble meiotic recombination�complexes. Genes Dev. 12: 2208-2221 [Abstract] [Full Text]