Merosin and laminin in myogenesis; specific requirement for merosin in myotube stability and survival

doi:10.1083/jcb.134.6.1483

This Article

	Full Text (PDF, 5027K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Vachon, P. H.
	Articles by Engvall, E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Vachon, P. H.
	Articles by Engvall, E.


Social Bookmarking

	What's this?

ARTICLES

Merosin and laminin in myogenesis; specific requirement for merosin in myotube stability and survival

PH Vachon, F Loechel, H Xu, UM Wewer and E Engvall
Burnham Institute (La Jolla Cancer Research Center), California 92037, USA.

Laminin (laminin-1; alpha 1-beta 1-gamma 1) is known to promote myoblast proliferation, fusion, and myotube formation. Merosin (laminin- 2 and -4; alpha 2-beta 1/beta 2-gamma 1) is the predominant laminin variant in skeletal muscle basement membranes; genetic defects affecting its structure or expression are the causes of some types of congenital muscular dystrophy. However, the precise nature of the functions of merosin in muscle remain unknown. We have developed an in vitro system that exploits human RD and mouse C2C12 myoblastic cell lines and their clonal variants to study the roles of merosin and laminin in myogenesis. In the parental cells, which fuse efficiently to multinucleated myotubes, merosin expression is upregulated as a function of differentiation while laminin expression is downregulated. Cells from fusion-deficient clones do not express either protein, but laminin or merosin added to the culture medium induced their fusion. Clonal variants which fuse, but form unstable myotubes, express laminin but not merosin. Exogenous merosin converted these myotubes to a stable phenotype, while laminin had no effect. Myotube instability was corrected most efficiently by transfection of the merosin-deficient cells with the merosin alpha 2 chain cDNA. Finally, merosin appears to promote myotube stability by preventing apoptosis. Hence, these studies identify novel biological functions for merosin in myoblast fusion and muscle cell survival; furthermore, these explain some of the pathogenic events observed in congenital muscular dystrophy caused by merosin deficiency and provide in vitro models to further investigate the molecular mechanisms of this disease.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Barop, J., Sauer, H., Steger, K., Wimmer, M. (2009). Differentiation-dependent PTPIP51 Expression in Human Skeletal Muscle Cell Culture. J. Histochem. Cytochem. 57: 425-435 [Abstract] [Full Text]
Liu, J., Burkin, D. J., Kaufman, S. J. (2008). Increasing {alpha}7{beta}1-integrin promotes muscle cell proliferation, adhesion, and resistance to apoptosis without changing gene expression. Am. J. Physiol. Cell Physiol. 294: C627-C640 [Abstract] [Full Text]
Badri, K. R., Zhou, Y., Schuger, L. (2008). Embryological Origin of Airway Smooth Muscle. Proc Am Thorac Soc 5: 4-10 [Abstract] [Full Text]
Meinen, S., Barzaghi, P., Lin, S., Lochmuller, H., Ruegg, M. A. (2007). Linker molecules between laminins and dystroglycan ameliorate laminin-{alpha}2-deficient muscular dystrophy at all disease stages. JCB 176: 979-993 [Abstract] [Full Text]
Sonnet, C., Lafuste, P., Arnold, L., Brigitte, M., Poron, F., Authier, F., Chretien, F., Gherardi, R. K., Chazaud, B. (2006). Human macrophages rescue myoblasts and myotubes from apoptosis through a set of adhesion molecular systems. J. Cell Sci. 119: 2497-2507 [Abstract] [Full Text]
Vitolo, D., Ciocci, L., Deriu, G., Spinelli, S., Cortese, S., Masuelli, L., Morrone, S., Filice, M. J., Coloni, G. F., Natali, P. G., Baroni, C. D. (2006). Laminin {alpha}2 Chain-Positive Vessels and Epidermal Growth Factor in Lung Neuroendocrine Carcinoma: A Model of a Novel Cooperative Role of Laminin-2 and Epidermal Growth Factor in Vessel Neoplastic Invasion and Metastasis. Am. J. Pathol. 168: 991-1003 [Abstract] [Full Text]
Hager, M., Gawlik, K., Nystrom, A., Sasaki, T., Durbeej, M. (2005). Laminin {alpha}1 Chain Corrects Male Infertility Caused by Absence of Laminin {alpha}2 Chain. Am. J. Pathol. 167: 823-833 [Abstract] [Full Text]
Qiao, C., Li, J., Zhu, T., Draviam, R., Watkins, S., Ye, X., Chen, C., Li, J., Xiao, X. (2005). Amelioration of laminin-{alpha}2-deficient congenital muscular dystrophy by somatic gene transfer of miniagrin. Proc. Natl. Acad. Sci. USA 102: 11999-12004 [Abstract] [Full Text]
Dominov, J. A., Kravetz, A. J., Ardelt, M., Kostek, C. A., Beermann, M. L., Miller, J. B. (2005). Muscle-specific BCL2 expression ameliorates muscle disease in laminin {alpha}2-deficient, but not in dystrophin-deficient, mice. Hum Mol Genet 14: 1029-1040 [Abstract] [Full Text]
Li, Z.-f., Shelton, G. D., Engvall, E. (2005). Elimination of Myostatin Does Not Combat Muscular Dystrophy in dy Mice but Increases Postnatal Lethality. Am. J. Pathol. 166: 491-497 [Abstract] [Full Text]
Dufour, G., Demers, M.-J., Gagne, D., Dydensborg, A. B., Teller, I. C., Bouchard, V., Degongre, I., Beaulieu, J.-F., Cheng, J. Q., Fujita, N., Tsuruo, T., Vallee, K., Vachon, P. H. (2004). Human Intestinal Epithelial Cell Survival and Anoikis: DIFFERENTIATION STATE-DISTINCT REGULATION AND ROLES OF PROTEIN KINASE B/Akt ISOFORMS. J. Biol. Chem. 279: 44113-44122 [Abstract] [Full Text]
Gawlik, K., Miyagoe-Suzuki, Y., Ekblom, P., Takeda, S., Durbeej, M. (2004). Laminin {alpha}1 chain reduces muscular dystrophy in laminin {alpha}2 chain deficient mice. Hum Mol Genet 13: 1775-1784 [Abstract] [Full Text]
Yuasa, K., Fukumoto, S., Kamasaki, Y., Yamada, A., Fukumoto, E., Kanaoka, K., Saito, K., Harada, H., Arikawa-Hirasawa, E., Miyagoe-Suzuki, Y., Takeda, S., Okamoto, K., Kato, Y., Fujiwara, T. (2004). Laminin {alpha}2 Is Essential for Odontoblast Differentiation Regulating Dentin Sialoprotein Expression. J. Biol. Chem. 279: 10286-10292 [Abstract] [Full Text]
Cachaco, A. S., Chuva de Sousa Lopes, S. M., Kuikman, I., Bajanca, F., Abe, K., Baudoin, C., Sonnenberg, A., Mummery, C. L., Thorsteinsdottir, S. (2003). Knock-in of integrin {beta}1D affects primary but not secondary myogenesis in mice. Development 130: 1659-1671 [Abstract] [Full Text]
Sanes, J. R. (2003). The Basement Membrane/Basal Lamina of Skeletal Muscle. J. Biol. Chem. 278: 12601-12604 [Full Text]
Disatnik, M.-H., Boutet, S. C., Lee, C. H., Mochly-Rosen, D., Rando, T. A. (2002). Sequential activation of individual PKC isozymes in integrin-mediated muscle cell spreading: a role for MARCKS in an integrin signaling pathway. J. Cell Sci. 115: 2151-2163 [Abstract] [Full Text]
Chamoux, E., Narcy, A., Lehoux, J.-G., Gallo-Payet, N. (2002). Fibronectin, Laminin, and Collagen IV as Modulators of Cell Behavior during Adrenal Gland Development in the Human Fetus. J. Clin. Endocrinol. Metab. 87: 1819-1828 [Abstract] [Full Text]
Beqaj, S., Jakkaraju, S., Mattingly, R. R., Pan, D., Schuger, L. (2002). High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis. JCB 156: 893-903 [Abstract] [Full Text]
Schreider, C., Peignon, G., Thenet, S., Chambaz, J., Pincon-Raymond, M. (2002). Integrin-mediated functional polarization of Caco-2 cells through E-cadherin--actin complexes. J. Cell Sci. 115: 543-552 [Abstract] [Full Text]
Huet, C., Li, Z.-F., Liu, H.-Z., Black, R. A., Galliano, M.-F., Engvall, E. (2001). Skeletal muscle cell hypertrophy induced by inhibitors of metalloproteases; myostatin as a potential mediator. Am. J. Physiol. Cell Physiol. 281: C1624-C1634 [Abstract] [Full Text]
Gauthier, R., Harnois, C., Drolet, J.-F., Reed, J. C., Vezina, A., Vachon, P. H. (2001). Human intestinal epithelial cell survival: differentiation state-specific control mechanisms. Am. J. Physiol. Cell Physiol. 280: C1540-C1554 [Abstract] [Full Text]
North, P. E., Waner, M., Mizeracki, A., Mrak, R. E., Nicholas, R., Kincannon, J., Suen, J. Y., Mihm, M. C. Jr (2001). A Unique Microvascular Phenotype Shared by Juvenile Hemangiomas and Human Placenta. Arch Dermatol 137: 559-570 [Abstract] [Full Text]
Cosgrove, D., Rodgers, K., Meehan, D., Miller, C., Bovard, K., Gilroy, A., Gardner, H., Kotelianski, V., Gotwals, P., Amatucci, A., Kalluri, R. (2000). Integrin {alpha}1{beta}1 and Transforming Growth Factor-{beta}1 Play Distinct Roles in Alport Glomerular Pathogenesis and Serve as Dual Targets for Metabolic Therapy. Am. J. Pathol. 157: 1649-1659 [Abstract] [Full Text]
Gilmore, A. P., Metcalfe, A. D., Romer, L. H., Streuli, C. H. (2000). Integrin-mediated Survival Signals Regulate the Apoptotic Function of Bax through Its Conformation and Subcellular Localization. JCB 149: 431-446 [Abstract] [Full Text]
Relan, N. K., Yang, Y., Beqaj, S., Miner, J. H., Schuger, L. (1999). Cell Elongation Induces Laminin {alpha}2 Chain Expression in Mouse Embryonic Mesenchymal Cells: Role in Visceral Myogenesis. JCB 147: 1341-1350 [Abstract] [Full Text]
Disatnik, M.-H., Rando, T. A. (1999). Integrin-mediated Muscle Cell Spreading. THE ROLE OF PROTEIN KINASE C IN OUTSIDE-IN AND INSIDE-OUT SIGNALING AND EVIDENCE OF INTEGRIN CROSS-TALK. J. Biol. Chem. 274: 32486-32492 [Abstract] [Full Text]
Tachibana, I., Hemler, M. E. (1999). Role of Transmembrane 4 Superfamily (TM4SF) Proteins CD9 and CD81 in Muscle Cell Fusion and Myotube Maintenance. JCB 146: 893-904 [Abstract] [Full Text]
Lamande, S. R., Shields, K. A., Kornberg, A. J., Shield, L. K., Bateman, J. F. (1999). Bethlem Myopathy and Engineered Collagen VI Triple Helical Deletions Prevent Intracellular Multimer Assembly and Protein Secretion. J. Biol. Chem. 274: 21817-21822 [Abstract] [Full Text]
Montanaro, F., Lindenbaum, M., Carbonetto, S. (1999). alpha -Dystroglycan Is a Laminin Receptor Involved in Extracellular Matrix Assembly on Myotubes and Muscle Cell Viability. JCB 145: 1325-1340 [Abstract] [Full Text]
Farrelly, N., Lee, Y.-J., Oliver, J., Dive, C., Streuli, C. H. (1999). Extracellular Matrix Regulates Apoptosis in Mammary Epithelium through a Control on Insulin Signaling. JCB 144: 1337-1348 [Abstract] [Full Text]
Brown, S., Fassati, A, Popplewell, L, Page, A., Henry, M., Campbell, K., Dickson, G (1999). Dystrophic phenotype induced in vitro by antibody blockade of muscle alpha-dystroglycan-laminin interaction. J. Cell Sci. 112: 209-216 [Abstract]
McDearmon, E. L., Burwell, A. L., Combs, A. C., Renley, B. A., Sdano, M. T., Ervasti, J. M. (1998). Differential Heparin Sensitivity of alpha -Dystroglycan Binding to Laminins Expressed in Normal and dy/dy Mouse Skeletal Muscle. J. Biol. Chem. 273: 24139-24144 [Abstract] [Full Text]
Gilpin, B. J., Loechel, F., Mattei, M.-G., Engvall, E., Albrechtsen, R., Wewer, U. M. (1998). A Novel, Secreted Form of Human ADAM 12�(Meltrin alpha ) Provokes Myogenesis in Vivo. J. Biol. Chem. 273: 157-166 [Abstract] [Full Text]
Patton, B. L., Miner, J. H., Chiu, A. Y., Sanes, J. R. (1997). Distribution and Function of Laminins in the Neuromuscular System of Developing, Adult, and Mutant Mice. JCB 139: 1507-1521 [Abstract] [Full Text]
Schuger, L., Skubitz, A. P.N., Zhang, J., Sorokin, L., He, L. (1997). Laminin alpha 1 Chain Synthesis in the Mouse Developing Lung: Requirement for Epithelial-Mesenchymal Contact and Possible Role in Bronchial Smooth muscle Development. JCB 139: 553-562 [Abstract] [Full Text]
Ervasti, J. M., Burwell, A. L., Geissler, A. L. (1997). Tissue-specific Heterogeneity in alpha -Dystroglycan Sialoglycosylation. SKELETAL MUSCLE alpha -DYSTROGLYCAN IS A LATENT RECEPTOR FOR VICIA VILLOSA AGGLUTININ B4 MASKED BY SIALIC ACID MODIFICATION. J. Biol. Chem. 272: 22315-22321 [Abstract] [Full Text]
Miner, J. H., Patton, B. L., Lentz, S. I., Gilbert, D. J., Snider, W. D., Jenkins, N. A., Copeland, N. G., Sanes, J. R. (1997). The Laminin alpha �Chains: Expression, Developmental Transitions, and Chromosomal Locations of alpha 1-5, Identification of Heterotrimeric Laminins 8-11, and Cloning of a Novel alpha 3 Isoform. JCB 137: 685-701 [Abstract] [Full Text]
Hodges, B., Hayashi, Y., Nonaka, I, Wang, W, Arahata, K, Kaufman, S. (1997). Altered expression of the alpha7beta1 integrin in human and murine muscular dystrophies. J. Cell Sci. 110: 2873-2881 [Abstract]
Gellon, G, Harding, K., McGinnis, N, Martin, M., McGinnis, W (1997). A genetic screen for modifiers of Deformed homeotic function identifies novel genes required for head development. Development 124: 3321-3331 [Abstract]
Yao, C., Ziober, B., Sutherland, A., Mendrick, D., Kramer, R. (1996). Laminins promote the locomotion of skeletal myoblasts via the alpha 7 integrin receptor. J. Cell Sci. 109: 3139-3150 [Abstract]
Beqaj, S., Jakkaraju, S., Mattingly, R. R., Pan, D., Schuger, L. (2002). High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis. JCB 156: 893-903 [Abstract] [Full Text]
Basque, J.-R., Chailler, P., Menard, D. (2002). Laminins and TGF-beta maintain cell polarity and functionality of human gastric glandular epithelium. Am. J. Physiol. Cell Physiol. 282: C873-C884 [Abstract] [Full Text]