In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation

doi:10.1083/jcb.135.2.329

This Article

	Full Text (PDF, 1578K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Shulga, N.
	Articles by Goldfarb, D. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Shulga, N.
	Articles by Goldfarb, D. S.


Social Bookmarking

	What's this?

ARTICLES

In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation

N Shulga, P Roberts, Z Gu, L Spitz, MM Tabb, M Nomura and DS Goldfarb
Department of Biology, University of Rochester, New York 14627, USA.

The transport of proteins into the nucleus is a receptor-mediated process that is likely to involve between 50-100 gene products, including many that comprise the nuclear pore complex. We have developed an assay in Saccharomyces cerevisiae for the nuclear transport of green fluorescent protein fused to the SV-40 large T antigen nuclear localization signal (NLS-GFP). This assay allows the measurement of relative NLS-GFP nuclear import rates in wild-type and mutant cells under various physiological conditions. Probably the best understood component of the nuclear transport apparatus is Srp1p, the NLS receptor, which binds NLS-cargo in the cytoplasm and accompanies it into the nucleus. When compared to SRP1+ cells, NLS-GFP import rates in temperature-sensitive srp1-31 cells were slower and showed a lower temperature optimum. The in vivo transport defect of the srp1-31 cells was correlated with the purified protein's thermal sensitivity, as assayed by in vitro NLS peptide binding. We show that the kinetics of NLS-directed nuclear transport in wild-type cells is stimulated by the elevated expression of SSA1, which encodes a cytoplasmic heat shock protein 70 (Hsp70). Elevated Hsp70 levels are sufficient to suppress the NLS-GFP import defects in srp1-31 and nup82-3 cells. NUP82 encodes a protein that functions within the nuclear pore complex subsequent to docking. These results provide genetic evidence that Hsp70 acts during both targeting and translocation phases of nuclear transport, possibly as a molecular chaperone to promote the formation and stability of the Srp1p-NLS-cargo complex.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Makio, T., Stanton, L. H., Lin, C.-C., Goldfarb, D. S., Weis, K., Wozniak, R. W. (2009). The nucleoporins Nup170p and Nup157p are essential for nuclear pore complex assembly. JCB 185: 459-473 [Abstract] [Full Text]
Dawson, T. R., Lazarus, M. D., Hetzer, M. W., Wente, S. R. (2009). ER membrane-bending proteins are necessary for de novo nuclear pore formation. JCB 184: 659-675 [Abstract] [Full Text]
Pulliam, K. F., Fasken, M. B., McLane, L. M., Pulliam, J. V., Corbett, A. H. (2009). The Classical Nuclear Localization Signal Receptor, Importin-{alpha}, Is Required for Efficient Transition Through the G1/S Stage of the Cell Cycle in Saccharomyces cerevisiae. Genetics 181: 105-118 [Abstract] [Full Text]
McLane, L. M., Pulliam, K. F., Devine, S. E., Corbett, A. H. (2008). The Ty1 integrase protein can exploit the classical nuclear protein import machinery for entry into the nucleus. Nucleic Acids Res 36: 4317-4326 [Abstract] [Full Text]
Cheng, X., Belshan, M., Ratner, L. (2008). Hsp40 Facilitates Nuclear Import of the Human Immunodeficiency Virus Type 2 Vpx-Mediated Preintegration Complex. J. Virol. 82: 1229-1237 [Abstract] [Full Text]
Terry, L. J., Wente, S. R. (2007). Nuclear mRNA export requires specific FG nucleoporins for translocation through the nuclear pore complex. JCB 178: 1121-1132 [Abstract] [Full Text]
Makhnevych, T., Ptak, C., Lusk, C. P., Aitchison, J. D., Wozniak, R. W. (2007). The role of karyopherins in the regulated sumoylation of septins. JCB 177: 39-49 [Abstract] [Full Text]
Ryan, K. J., Zhou, Y., Wente, S. R. (2007). The Karyopherin Kap95 Regulates Nuclear Pore Complex Assembly into Intact Nuclear Envelopes In Vivo. Mol. Biol. Cell 18: 886-898 [Abstract] [Full Text]
Timney, B. L., Tetenbaum-Novatt, J., Agate, D. S., Williams, R., Zhang, W., Chait, B. T., Rout, M. P. (2006). Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. JCB 175: 579-593 [Abstract] [Full Text]
Hodel, A. E., Harreman, M. T., Pulliam, K. F., Harben, M. E., Holmes, J. S., Hodel, M. R., Berland, K. M., Corbett, A. H. (2006). Nuclear Localization Signal Receptor Affinity Correlates with in Vivo Localization in Saccharomyces cerevisiae. J. Biol. Chem. 281: 23545-23556 [Abstract] [Full Text]
Miao, M., Ryan, K. J., Wente, S. R. (2006). The Integral Membrane Protein Pom34p Functionally Links Nucleoporin Subcomplexes. Genetics 172: 1441-1457 [Abstract] [Full Text]
Belanger, K. D., Gupta, A., MacDonald, K. M., Ott, C. M., Hodge, C. A., Cole, C. M., Davis, L. I. (2005). Nuclear Pore Complex Function in Saccharomyces cerevisiae Is Influenced by Glycosylation of the Transmembrane Nucleoporin Pom152p. Genetics 171: 935-947 [Abstract] [Full Text]
Kose, S., Furuta, M., Koike, M., Yoneda, Y., Imamoto, N. (2005). The 70-kD heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors. JCB 171: 19-25 [Abstract] [Full Text]
Scott, R. J., Lusk, C. P., Dilworth, D. J., Aitchison, J. D., Wozniak, R. W. (2005). Interactions between Mad1p and the Nuclear Transport Machinery in the Yeast Saccharomyces cerevisiae. Mol. Biol. Cell 16: 4362-4374 [Abstract] [Full Text]
Wang, Y., Chen, W., Simpson, D. M., Elion, E. A. (2005). Cdc24 Regulates Nuclear Shuttling and Recruitment of the Ste5 Scaffold to a Heterotrimeric G Protein in Saccharomyces cerevisiae. J. Biol. Chem. 280: 13084-13096 [Abstract] [Full Text]
Zeitler, B., Weis, K. (2004). The FG-repeat asymmetry of the nuclear pore complex is dispensable for bulk nucleocytoplasmic transport in vivo. JCB 167: 583-590 [Abstract] [Full Text]
Belanger, K. D., Simmons, L. A., Roth, J. K., VanderPloeg, K. A., Lichten, L. B., Fahrenkrog, B. (2004). The Karyopherin Msn5/Kap142 Requires Nup82 for Nuclear Export and Performs a Function Distinct from Translocation in RPA Protein Import. J. Biol. Chem. 279: 43530-43539 [Abstract] [Full Text]
Iordanskiy, S., Zhao, Y., DiMarzio, P., Agostini, I., Dubrovsky, L., Bukrinsky, M. (2004). Heat-shock protein 70 exerts opposing effects on Vpr-dependent and Vpr-independent HIV-1 replication in macrophages. Blood 104: 1867-1872 [Abstract] [Full Text]
Xu, Q., Li, S., Zhao, Y., Maures, T. J., Yin, P., Duan, C. (2004). Evidence That IGF Binding Protein-5 Functions as a Ligand-Independent Transcriptional Regulator in Vascular Smooth Muscle Cells. Circ. Res. 94: e46-e54 [Abstract] [Full Text]
Tsukahara, F., Maru, Y. (2004). Identification of Novel Nuclear Export and Nuclear Localization-related Signals in Human Heat Shock Cognate Protein 70. J. Biol. Chem. 279: 8867-8872 [Abstract] [Full Text]
Gilchrist, D., Rexach, M. (2003). Molecular Basis for the Rapid Dissociation of Nuclear Localization Signals from Karyopherin {alpha} in the Nucleoplasm. J. Biol. Chem. 278: 51937-51949 [Abstract] [Full Text]
Pyhtila, B., Rexach, M. (2003). A Gradient of Affinity for the Karyopherin Kap95p along the Yeast Nuclear Pore Complex. J. Biol. Chem. 278: 42699-42709 [Abstract] [Full Text]
Gao, H., Sumanaweera, N., Bailer, S. M., Stochaj, U. (2003). Nuclear Accumulation of the Small GTPase Gsp1p Depends on Nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the Acetyl-CoA Carboxylase Acc1p. J. Biol. Chem. 278: 25331-25340 [Abstract] [Full Text]
Shulga, N., Goldfarb, D. S. (2003). Binding Dynamics of Structural Nucleoporins Govern Nuclear Pore Complex Permeability and May Mediate Channel Gating. Mol. Cell. Biol. 23: 534-542 [Abstract] [Full Text]
Stade, K., Vogel, F., Schwienhorst, I., Meusser, B., Volkwein, C., Nentwig, B., Dohmen, R. J., Sommer, T. (2002). A Lack of SUMO Conjugation Affects cNLS-dependent Nuclear Protein Import in Yeast. J. Biol. Chem. 277: 49554-49561 [Abstract] [Full Text]
Aoki, K., Kragler, F., Xoconostle-Cazares, B., Lucas, W. J. (2002). A subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata. Proc. Natl. Acad. Sci. USA 99: 16342-16347 [Abstract] [Full Text]
Iouk, T., Kerscher, O., Scott, R. J., Basrai, M. A., Wozniak, R. W. (2002). The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint. JCB 159: 807-819 [Abstract] [Full Text]
Ansari, H., Greco, G., Luban, J. (2002). Cyclophilin A Peptidyl-Prolyl Isomerase Activity Promotes Zpr1 Nuclear Export. Mol. Cell. Biol. 22: 6993-7003 [Abstract] [Full Text]
Isoyama, T., Kuge, S., Nomoto, A. (2002). The Core Protein of Hepatitis C Virus Is Imported into the Nucleus by Transport Receptor Kap123p but Inhibits Kap121p-dependent Nuclear Import of Yeast AP1-like Transcription Factor in Yeast Cells. J. Biol. Chem. 277: 39634-39641 [Abstract] [Full Text]
Yu, L.-G., Andrews, N., Weldon, M., Gerasimenko, O. V., Campbell, B. J., Singh, R., Grierson, I., Petersen, O. H., Rhodes, J. M. (2002). An N-terminal Truncated Form of Orp150 Is a Cytoplasmic Ligand for the Anti-proliferative Mushroom Agaricus bisporus Lectin and Is Required for Nuclear Localization Sequence-dependent Nuclear Protein Import. J. Biol. Chem. 277: 24538-24545 [Abstract] [Full Text]
Schwoebel, E. D., Ho, T. H., Moore, M. S. (2002). The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion. JCB 157: 963-974 [Abstract] [Full Text]
Gilchrist, D., Mykytka, B., Rexach, M. (2002). Accelerating the Rate of Disassembly of Karyopherin{middle dot}Cargo Complexes. J. Biol. Chem. 277: 18161-18172 [Abstract] [Full Text]
Franke, J., Reimann, B., Hartmann, E., Kohler, M., Wiedmann, B. (2002). Evidence for a nuclear passage of nascent polypeptide-associated complex subunits in yeast. J. Cell Sci. 114: 2641-2648 [Abstract] [Full Text]
Quimby, B. B., Leung, S. W., Bayliss, R., Harreman, M. T., Thirumala, G., Stewart, M., Corbett, A. H. (2001). Functional Analysis of the Hydrophobic Patch on Nuclear Transport Factor 2 Involved in Interactions with the Nuclear Pore in Vivo. J. Biol. Chem. 276: 38820-38829 [Abstract] [Full Text]
Miller, M. E., Cross, F. R. (2001). Mechanisms Controlling Subcellular Localization of the G1 Cyclins Cln2p and Cln3p in Budding Yeast. Mol. Cell. Biol. 21: 6292-6311 [Abstract] [Full Text]
Denning, D., Mykytka, B., Allen, N. P.C., Huang, L., Al Burlingame, , Rexach, M. (2001). The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex. JCB 154: 937-950 [Abstract] [Full Text]
Dilworth, D. J., Suprapto, A., Padovan, J. C., Chait, B. T., Wozniak, R. W., Rout, M. P., Aitchison, J. D. (2001). Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex. JCB 153: 1465-1478 [Abstract] [Full Text]
Marelli, M., Lusk, C. P., Chan, H., Aitchison, J. D., Wozniak, R. W. (2001). A Link between the Synthesis of Nucleoporins and the Biogenesis of the Nuclear Envelope. JCB 153: 709-724 [Abstract] [Full Text]
Grosshans, H., Deinert, K., Hurt, E., Simos, G. (2001). Biogenesis of the Signal Recognition Particle (Srp) Involves Import of Srp Proteins into the Nucleolus, Assembly with the Srp-Rna, and Xpo1p-Mediated Export. JCB 153: 745-762 [Abstract] [Full Text]
Künzler, M., Trueheart, J., Sette, C., Hurt, E., Thorner, J. (2001). Mutations in the YRB1 Gene Encoding Yeast Ran-Binding-Protein-1 That Impair Nucleocytoplasmic Transport and Suppress Yeast Mating Defects. Genetics 157: 1089-1105 [Abstract] [Full Text]
Fahrenkrog, B., Hübner, W., Mandinova, A., Panté, N., Keller, W., Aebi, U. (2000). The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import. Mol. Biol. Cell 11: 3885-3896 [Abstract] [Full Text]
McClellan, A. J., Brodsky, J. L. (2000). Mutation of the ATP-Binding Pocket of SSA1 Indicates That a Functional Interaction Between Ssa1p and Ydj1p Is Required for Post-translational Translocation Into the Yeast Endoplasmic Reticulum. Genetics 156: 501-512 [Abstract] [Full Text]
Tabb, M. M., Tongaonkar, P., Vu, L., Nomura, M. (2000). Evidence for Separable Functions of Srp1p, the Yeast Homolog of Importin alpha (Karyopherin alpha ): Role for Srp1p and Sts1p in Protein Degradation. Mol. Cell. Biol. 20: 6062-6073 [Abstract] [Full Text]
Quimby, B. B., Wilson, C. A., Corbett, A. H. (2000). The Interaction Between Ran and NTF2 is Required for Cell Cycle Progression. Mol. Biol. Cell 11: 2617-2629 [Abstract] [Full Text]
Shulga, N., Mosammaparast, N., Wozniak, R., Goldfarb, D. S. (2000). Yeast Nucleoporins Involved in Passive Nuclear Envelope Permeability. JCB 149: 1027-1038 [Abstract] [Full Text]
Rout, M. P., Aitchison, J. D., Suprapto, A., Hjertaas, K., Zhao, Y., Chait, B. T. (2000). The Yeast Nuclear Pore Complex: Composition, Architecture, and Transport Mechanism. JCB 148: 635-652 [Abstract] [Full Text]
Saphire, A. C. S., Guan, T., Schirmer, E. C., Nemerow, G. R., Gerace, L. (2000). Nuclear Import of Adenovirus DNA in Vitro Involves the Nuclear Protein Import Pathway and hsc70. J. Biol. Chem. 275: 4298-4304 [Abstract] [Full Text]
Jagiello, I, Van Eynde, A, Vulsteke, V, Beullens, M, Boudrez, A, Keppens, S, Stalmans, W, Bollen, M (2000). Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1. J. Cell Sci. 113: 3761-3768 [Abstract]
Marchetti, M., Tschudi, C, Kwon, H, Wolin, S., Ullu, E (2000). Import of proteins into the trypanosome nucleus and their distribution at karyokinesis. J. Cell Sci. 113: 899-906 [Abstract]
Liu, J., DeFranco, D. B. (2000). Protracted Nuclear Export of Glucocorticoid Receptor Limits Its Turnover and Does Not Require the Exportin 1/CRM1-Directed Nuclear Export Pathway. Mol. Endocrinol. 14: 40-51 [Abstract] [Full Text]
Nanduri, J., Mitra, S., Andrei, C., Liu, Y., Yu, Y., Hitomi, M., Tartakoff, A. M. (1999). An Unexpected Link between the Secretory Path and the Organization of the Nucleus. J. Biol. Chem. 274: 33785-33789 [Abstract] [Full Text]
York, J. D., Odom, A. R., Murphy, R., Ives, E. B., Wente, S. R. (1999). A Phospholipase C-Dependent Inositol Polyphosphate Kinase Pathway Required for Efficient Messenger RNA Export. Science 285: 96-100 [Abstract] [Full Text]
Whalen, W. A., Yoon, J. H., Shen, R., Dhar, R. (1999). Regulation of mRNA Export by Nutritional Status in Fission Yeast. Genetics 152: 827-838 [Abstract] [Full Text]
Shibuya, T., Tsuneyoshi, S., Azad, A. K., Urushiyama, S., Ohshima, Y., Tani, T. (1999). Characterization of the ptr6+ Gene in Fission Yeast: A Possible Involvement of a Transcriptional Coactivator TAF in Nucleocytoplasmic Transport of mRNA. Genetics 152: 869-880 [Abstract] [Full Text]
Liu, Y., Guo, W., Tartakoff, P. Y., Tartakoff, A. M. (1999). A Crm1p-independent nuclear export path for the mRNA-associated protein, Npl3p/Mtr13p. Proc. Natl. Acad. Sci. USA 96: 6739-6744 [Abstract] [Full Text]
Shulga, N., James, P., Craig, E. A., Goldfarb, D. S. (1999). A Nuclear Export Signal Prevents Saccharomyces cerevisiae Hsp70 Ssb1p from Stimulating Nuclear Localization Signal-directed Nuclear Transport. J. Biol. Chem. 274: 16501-16507 [Abstract] [Full Text]
Strambio-de-Castillia, C., Blobel, G., Rout, M. P. (1999). Proteins Connecting the Nuclear Pore Complex with the Nuclear Interior. JCB 144: 839-855 [Abstract] [Full Text]
Suttner, D. M., Sridhar, K., Lee, C. S., Tomura, T., Hansen, T. N., Dennery, P. A. (1999). Protective effects of transient HO-1 overexpression on susceptibility to oxygen toxicity in lung cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L443-L451 [Abstract] [Full Text]
Yu, L.-G., Fernig, D. G., White, M. R.�H., Spiller, D. G., Appleton, P., Evans, R. C., Grierson, I., Smith, J. A., Davies, H., Gerasimenko, O. V., Petersen, O. H., Milton, J. D., Rhodes, J. M. (1999). Edible Mushroom (Agaricus bisporus) Lectin, Which Reversibly Inhibits Epithelial Cell Proliferation, Blocks Nuclear Localization Sequence-dependent Nuclear Protein Import. J. Biol. Chem. 274: 4890-4899 [Abstract] [Full Text]
Albertini, M., Pemberton, L. F., Rosenblum, J. S., Blobel, G. (1998). A Novel Nuclear Import Pathway for the Transcription Factor TFIIS. JCB 143: 1447-1455 [Abstract] [Full Text]
Belgareh, N., Snay-Hodge, C., Pasteau, F., Dagher, S., Cole, C. N., Doye, V. (1998). Functional Characterization of a Nup159p-containing Nuclear Pore Subcomplex. Mol. Biol. Cell 9: 3475-3492 [Abstract] [Full Text]
Solsbacher, J., Maurer, P., Bischoff, F. R., Schlenstedt, G. (1998). Cse1p Is Involved in Export of Yeast Importin alpha �from the Nucleus. Mol. Cell. Biol. 18: 6805-6815 [Abstract] [Full Text]
Stutz, F., Rosbash, M. (1998). Nuclear RNA export. Genes Dev. 12: 3303-3319 [Full Text]
Hurwitz, M. E., Strambio-de-Castillia, C., Blobel, G. (1998). Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Proc. Natl. Acad. Sci. USA 95: 11241-11245 [Abstract] [Full Text]
Hettema, E. H., Ruigrok, C. C.M., Koerkamp, M. G., van den Berg, M., Tabak, H. F., Distel, B., Braakman, I. (1998). The Cytosolic DnaJ-like Protein Djp1p Is Involved Specifically in Peroxisomal Protein Import. JCB 142: 421-434 [Abstract] [Full Text]
Pan, X, Goldfarb, D. (1998). YEB3/VAC8 encodes a myristylated armadillo protein of the Saccharomyces cerevisiae vacuolar membrane that functions in vacuole fusion and inheritance. J. Cell Sci. 111: 2137-2147 [Abstract]
Pemberton, L. F., Rosenblum, J. S., Blobel, G. (1997). A Distinct and Parallel Pathway for the Nuclear Import of an mRNA-binding Protein. JCB 139: 1645-1653 [Abstract] [Full Text]
Rosenblum, J. S., Pemberton, L. F., Blobel, G. (1997). A Nuclear Import Pathway for a Protein Involved in tRNA Maturation. JCB 139: 1655-1661 [Abstract] [Full Text]
Grandi, P., Dang, T., Pané, N., Shevchenko, A., Mann, M., Forbes, D., Hurt, E. (1997). Nup93, a Vertebrate Homologue of Yeast Nic96p, Forms a Complex with a Novel 205-kDa Protein and Is Required for Correct Nuclear Pore Assembly. Mol. Biol. Cell 8: 2017-2038 [Abstract] [Full Text]
Seedorf, M., Silver, P. A. (1997). Importin/karyopherin protein family members required for mRNA export from the�nucleus. Proc. Natl. Acad. Sci. USA 94: 8590-8595 [Abstract] [Full Text]
Del Priore, V, Heath, C, Snay, C, MacMillan, A, Gorsch, L, Dagher, S, Cole, C (1997). A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae. J. Cell Sci. 110: 2987-2999 [Abstract]
Shan, X, Xue, Z, Euskirchen, G, Melese, T (1997). NNF1 is an essential yeast gene required for proper spindle orientation, nucleolar and nuclear envelope structure and mRNA export. J. Cell Sci. 110: 1615-1624 [Abstract]
Schedlich, L. J., Le Page, S. L., Firth, S. M., Briggs, L. J., Jans, D. A., Baxter, R. C. (2000). Nuclear Import of Insulin-like Growth Factor-binding Protein-3 and -5 Is Mediated by the Importin beta Subunit. J. Biol. Chem. 275: 23462-23470 [Abstract] [Full Text]
Quimby, B. B., Lamitina, T., L'Hernault, S. W., Corbett, A. H. (2000). The Mechanism of Ran Import into the Nucleus by Nuclear Transport Factor 2. J. Biol. Chem. 275: 28575-28582 [Abstract] [Full Text]
Horton, L. E., James, P., Craig, E. A., Hensold, J. O. (2001). The Yeast hsp70 Homologue Ssa Is Required for Translation and Interacts with Sis1 and Pab1 on Translating Ribosomes. J. Biol. Chem. 276: 14426-14433 [Abstract] [Full Text]
Chughtai, Z. S., Rassadi, R., Matusiewicz, N., Stochaj, U. (2001). Starvation Promotes Nuclear Accumulation of the hsp70 Ssa4p in Yeast Cells. J. Biol. Chem. 276: 20261-20266 [Abstract] [Full Text]
Allen, N. P. C., Huang, L., Burlingame, A., Rexach, M. (2001). Proteomic Analysis of Nucleoporin Interacting Proteins. J. Biol. Chem. 276: 29268-29274 [Abstract] [Full Text]
Baker, R. P., Harreman, M. T., Eccleston, J. F., Corbett, A. H., Stewart, M. (2001). Interaction between Ran and Mog1 Is Required for Efficient Nuclear Protein Import. J. Biol. Chem. 276: 41255-41262 [Abstract] [Full Text]