A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding

doi:10.1083/jcb.135.5.1239

This Article

Full Text (PDF, 1369K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Sohn, K.

Articles by Wieland, F. T.

Search for Related Content

PubMed

PubMed Citation

Articles by Sohn, K.

Articles by Wieland, F. T.

Pubmed/NCBI databases

Gene	Protein
UniGene
Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding

K Sohn, L Orci, M Ravazzola, M Amherdt, M Bremser, F Lottspeich, K Fiedler, JB Helms and FT Wieland
Institut fur Biochemie I, Universitat Heidelberg, Germany.

Formation of non-clathrin-coated vesicles requires the recruitment of several cytosolic factors to the Golgi membrane. To identify membrane proteins involved in this budding process, a highly abundant type I transmembrane protein (p23) was isolated from mammalian Golgi-derived COPI-coated vesicles, and its cDNA was cloned and sequenced. It belongs to the p24 family of proteins involved in the budding of transport vesicles (Stamnes, M.A., M.W. Craighead, M.H. Hoe, N. Lampen, S. Geromanos, P. Tempst, and J.E. Rothman. 1995. Proc. Natl. Acad. Sci. USA. 92:8011-8015). p23 consists of a large NH2-terminal luminal domain and a short COOH-terminal cytoplasmic tail (-LRRFFKAKKLIE-CO2-) that shows similarity, but not identity, with the sequence motif-KKXX-CO2-, known as a signal for retrieval of escaped ER-resident membrane proteins (Jackson, M.R., T. Nilsson, and P.A. Peterson. 1990. EMBO (Eur. Mol. Biol. Organ.) J. 9:3153-3162; Nilsson, T., M. Jackson, and P.A. Peterson. 1989. Cell. 58:707-718). The cytoplasmic tail of p23 binds to coatomer with similar efficiency as known KKXX motifs. However, the p23 tail differs from the KKXX motif in having an additional motif needed for binding of coatomer. p23 is localized to Golgi cisternae and, during vesicle formation, it concentrates into COPI-coated buds and vesicles. Biochemical analysis revealed that p23 is enriched in vesicles by a factor of approximately 20, as compared with the donor Golgi fraction, and is present in amounts stoichiometric to the small GTP-binding protein ADP-ribosylation factor (ARF) and coatomer. From these data we conclude that p23 represents a Golgi- specific receptor for coatomer involved in the formation of COPI-coated vesicles.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Strating, J. R.P.M., van Bakel, N. H.M., Leunissen, J. A.M., Martens, G. J.M. (2009). A Comprehensive Overview of the Vertebrate p24 Family: Identification of a Novel Tissue-Specifically Expressed Member. Mol Biol Evol 26: 1707-1714 [Abstract] [Full Text]
Beck, R., Sun, Z., Adolf, F., Rutz, C., Bassler, J., Wild, K., Sinning, I., Hurt, E., Brugger, B., Bethune, J., Wieland, F. (2008). Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc. Natl. Acad. Sci. USA 105: 11731-11736 [Abstract] [Full Text]
Hwang, S. O., Boswell, S. A., Seo, J.-S., Lee, S. W. (2008). Novel Oxidative Stress-responsive Gene ERS25 Functions as a Regulator of the Heat-shock and Cell Death Response. J. Biol. Chem. 283: 13063-13069 [Abstract] [Full Text]
Mitrovic, S., Ben-Tekaya, H., Koegler, E., Gruenberg, J., Hauri, H.-P. (2008). The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi. Mol. Biol. Cell 19: 1976-1990 [Abstract] [Full Text]
Aguilera-Romero, A., Kaminska, J., Spang, A., Riezman, H., Muniz, M. (2008). The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus. JCB 180: 713-720 [Abstract] [Full Text]
Shrivastava-Ranjan, P., Faundez, V., Fang, G., Rees, H., Lah, J. J., Levey, A. I., Kahn, R. A. (2008). Mint3/X11{gamma} Is an ADP-Ribosylation Factor-dependent Adaptor that Regulates the Traffic of the Alzheimer's Precursor Protein from the Trans-Golgi Network. Mol. Biol. Cell 19: 51-64 [Abstract] [Full Text]
Li, X., Kaloyanova, D., van Eijk, M., Eerland, R., van der Goot, G., Oorschot, V., Klumperman, J., Lottspeich, F., Starkuviene, V., Wieland, F. T., Helms, J. B. (2007). Involvement of a Golgi-resident GPI-anchored Protein in Maintenance of the Golgi Structure. Mol. Biol. Cell 18: 1261-1271 [Abstract] [Full Text]
Hosaka, M., Watanabe, T., Yamauchi, Y., Sakai, Y., Suda, M., Mizutani, S., Takeuchi, T., Isobe, T., Izumi, T. (2007). A Subset of p23 Localized on Secretory Granules in Pancreatic {beta}-cells. J. Histochem. Cytochem. 55: 235-245 [Abstract] [Full Text]
Bethune, J., Kol, M., Hoffmann, J., Reckmann, I., Brugger, B., Wieland, F. (2006). Coatomer, the Coat Protein of COPI Transport Vesicles, Discriminates Endoplasmic Reticulum Residents from p24 Proteins. Mol. Cell. Biol. 26: 8011-8021 [Abstract] [Full Text]
Gupta, V., Swarup, G. (2006). Evidence for a role of transmembrane protein p25 in localization of protein tyrosine phosphatase TC48 to the ER. J. Cell Sci. 119: 1703-1714 [Abstract] [Full Text]
Lee, S. Y., Yang, J.-S., Hong, W., Premont, R. T., Hsu, V. W. (2005). ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation. JCB 168: 281-290 [Abstract] [Full Text]
Contreras, I., Yang, Y., Robinson, D. G., Aniento, F. (2004). Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat. Plant Cell Physiol 45: 1779-1786 [Abstract] [Full Text]
Bouw, G., Van Huizen, R., Jansen, E. J.R., Martens, G. J.M. (2004). A Cell-Specific Transgenic Approach in Xenopus Reveals the Importance of a Functional p24 System for a Secretory Cell. Mol. Biol. Cell 15: 1244-1253 [Abstract] [Full Text]
Emery, G., Parton, R. G., Rojo, M., Gruenberg, J. (2003). The trans-membrane protein p25 forms highly specialized domains that regulate membrane composition and dynamics. J. Cell Sci. 116: 4821-4832 [Abstract] [Full Text]
Kruljac-Letunic, A., Moelleken, J., Kallin, A., Wieland, F., Blaukat, A. (2003). The Tyrosine Kinase Pyk2 Regulates Arf1 Activity by Phosphorylation and Inhibition of the Arf-GTPase-activating Protein ASAP1. J. Biol. Chem. 278: 29560-29570 [Abstract] [Full Text]
Reinhard, C., Schweikert, M., Wieland, F. T., Nickel, W. (2003). Functional reconstitution of COPI coat assembly and disassembly using chemically defined components. Proc. Natl. Acad. Sci. USA 100: 8253-8257 [Abstract] [Full Text]
Jenne, N., Frey, K., Brugger, B., Wieland, F. T. (2002). Oligomeric State and Stoichiometry of p24 Proteins in the Early Secretory Pathway. J. Biol. Chem. 277: 46504-46511 [Abstract] [Full Text]
Engling, A., Backhaus, R., Stegmayer, C., Zehe, C., Seelenmeyer, C., Kehlenbach, A., Schwappach, B., Wegehingel, S., Nickel, W. (2002). Biosynthetic FGF-2 is targeted to non-lipid raft microdomains following translocation to the extracellular surface of CHO cells. J. Cell Sci. 115: 3619-3631 [Abstract] [Full Text]
Nickel, W., Brugger, B., Wieland, F. T. (2002). Vesicular transport: the core machinery of COPI recruitment and budding. J. Cell Sci. 115: 3235-3240 [Abstract] [Full Text]
Powers, J., Barlowe, C. (2002). Erv14p Directs a Transmembrane Secretory Protein into COPII-coated Transport Vesicles. Mol. Biol. Cell 13: 880-891 [Abstract] [Full Text]
Eberle, H. B., Serrano, R. L., Fullekrug, J., Schlosser, A., Lehmann, W. D., Lottspeich, F., Kaloyanova, D., Wieland, F. T., Helms, J. B. (2002). Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex. J. Cell Sci. 115: 827-838 [Abstract] [Full Text]
Szafer, E., Rotman, M., Cassel, D. (2001). Regulation of GTP Hydrolysis on ADP-ribosylation Factor-1 at the Golgi Membrane. J. Biol. Chem. 276: 47834-47839 [Abstract] [Full Text]
Barr, F. A., Preisinger, C., Kopajtich, R., Korner, R. (2001). Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus. JCB 155: 885-892 [Abstract] [Full Text]
Belden, W. J., Barlowe, C. (2001). Distinct Roles for the Cytoplasmic Tail Sequences of Emp24p and Erv25p in Transport between the Endoplasmic Reticulum and Golgi Complex. J. Biol. Chem. 276: 43040-43048 [Abstract] [Full Text]
Gkantiragas, I., Brugger, B., Stuven, E., Kaloyanova, D., Li, X.-Y., Lohr, K., Lottspeich, F., Wieland, F. T., Helms, J. B. (2001). Sphingomyelin-enriched Microdomains at the Golgi Complex. Mol. Biol. Cell 12: 1819-1833 [Abstract] [Full Text]
Dahm, T., White, J., Grill, S., Füllekrug, J., Stelzer, E. H.K. (2001). Quantitative ER {left-right-arrow} Golgi Transport Kinetics and Protein Separation upon Golgi Exit Revealed by Vesicular Integral Membrane Protein 36 Dynamics in Live Cells. Mol. Biol. Cell 12: 1481-1498 [Abstract] [Full Text]
Brugger, B., Sandhoff, R., Wegehingel, S., Gorgas, K., Malsam, J., Helms, J. B., Lehmann, W.-D., Nickel, W., Wieland, F. T. (2000). Evidence for Segregation of Sphingomyelin and Cholesterol during Formation of Copi-Coated Vesicles. JCB 151: 507-518 [Abstract] [Full Text]
Sullivan, B. M., Harrison-Lavoie, K. J., Marshansky, V., Lin, H. Y., Kehrl, J. H., Ausiello, D. A., Brown, D., Druey, K. M. (2000). RGS4 and RGS2 Bind Coatomer and Inhibit COPI Association with Golgi Membranes and Intracellular Transport. Mol. Biol. Cell 11: 3155-3168 [Abstract] [Full Text]
Kaiser, C. (2000). Thinking about p24 proteins and how transport vesicles select their cargo. Proc. Natl. Acad. Sci. USA 97: 3783-3785 [Full Text]
Muniz, M., Nuoffer, C., Hauri, H.-P., Riezman, H. (2000). The Emp24 Complex Recruits a Specific Cargo Molecule into Endoplasmic Reticulum-Derived Vesicles. JCB 148: 925-930 [Abstract] [Full Text]
Emery, G, Rojo, M, Gruenberg, J (2000). Coupled transport of p24 family members. J. Cell Sci. 113: 2507-2516 [Abstract]
Rojo, M, Emery, G, Marjomaki, V, McDowall, A., Parton, R., Gruenberg, J (2000). The transmembrane protein p23 contributes to the organization of the Golgi apparatus. J. Cell Sci. 113: 1043-1057 [Abstract]
Pepperkok, R, Whitney, J., Gomez, M, Kreis, T. (2000). COPI vesicles accumulating in the presence of a GTP restricted arf1 mutant are depleted of anterograde and retrograde cargo. J. Cell Sci. 113: 135-144 [Abstract]
Kuiper, R. P., Waterham, H. R., Rötter, J., Bouw, G., Martens, G. J. M. (2000). Differential Induction of Two p24delta Putative Cargo Receptors upon Activation of a Prohormoneproducing Cell. Mol. Biol. Cell 11: 131-140 [Abstract] [Full Text]
White, J., Johannes, L., Mallard, F., Girod, A., Grill, S., Reinsch, S., Keller, P., Tzschaschel, B., Echard, A., Goud, B., Stelzer, E. H.K. (1999). Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells. JCB 147: 743-760 [Abstract] [Full Text]
Wen, C., Greenwald, I. (1999). p24 Proteins and Quality Control of LIN-12 and GLP-1 Trafficking in Caenorhabditis elegans. JCB 145: 1165-1175 [Abstract] [Full Text]
Marzioch, M., Henthorn, D. C., Herrmann, J. M., Wilson, R., Thomas, D. Y., Bergeron, J. J.�M., Solari, R. C.�E., Rowley, A. (1999). Erp1p and Erp2p, Partners for Emp24p and Erv25p in a Yeast p24 Complex. Mol. Biol. Cell 10: 1923-1938 [Abstract] [Full Text]
Füllekrug, J., Suganuma, T., Tang, B. L., Hong, W., Storrie, B., Nilsson, T. (1999). Localization and Recycling of gp27 (hp24gamma 3): Complex Formation with Other p24 Family Members. Mol. Biol. Cell 10: 1939-1955 [Abstract] [Full Text]
Zhao, L., Helms, J. B., Brunner, J., Wieland, F. T. (1999). GTP-dependent Binding of ADP-ribosylation Factor to Coatomer in Close Proximity to the Binding Site for Dilysine Retrieval Motifs and p23. J. Biol. Chem. 274: 14198-14203 [Abstract] [Full Text]
Heimann, K., Percival, J. M., Weinberger, R., Gunning, P., Stow, J. L. (1999). Specific Isoforms of Actin-binding Proteins on Distinct Populations of Golgi-derived Vesicles. J. Biol. Chem. 274: 10743-10750 [Abstract] [Full Text]
Barz, W. P., Walter, P. (1999). Two Endoplasmic Reticulum () Membrane Proteins That Facilitate ER-to-Golgi Transport of Glycosylphosphatidylinositol-anchored Proteins. Mol. Biol. Cell 10: 1043-1059 [Abstract] [Full Text]
Pagano, A., Letourneur, F., Garcia-Estefania, D., Carpentier, J.-L., Orci, L., Paccaud, J.-P. (1999). Sec24 Proteins and Sorting at the Endoplasmic Reticulum. J. Biol. Chem. 274: 7833-7840 [Abstract] [Full Text]
Reinhard, C., Harter, C., Bremser, M., Brugger, B., Sohn, K., Helms, J. B., Wieland, F. (1999). Receptor-induced polymerization of coatomer. Proc. Natl. Acad. Sci. USA 96: 1224-1228 [Abstract] [Full Text]
Blum, R, Pfeiffer, F, Feick, P, Nastainczyk, W, Kohler, B, Schafer, K., Schulz, I (1999). Intracellular localization and in vivo trafficking of p24A and p23. J. Cell Sci. 112: 537-548 [Abstract]
Nakamura, N., Yamazaki, S., Sato, K., Nakano, A., Sakaguchi, M., Mihara, K. (1998). Identification of Potential Regulatory Elements for the Transport of Emp24p. Mol. Biol. Cell 9: 3493-3503 [Abstract] [Full Text]
Stamnes, M., Schiavo, G., Stenbeck, G., Sollner, T. H., Rothman, J. E. (1998). ADP-ribosylation factor and phosphatidic acid levels in Golgi membranes during budding of coatomer-coated vesicles. Proc. Natl. Acad. Sci. USA 95: 13676-13680 [Abstract] [Full Text]
Majoul, I., Sohn, K., Wieland, F. T., Pepperkok, R., Pizza, M., Hillemann, J., Soling, H.-D. (1998). KDEL Receptor (Erd2p)-mediated Retrograde Transport of the Cholera Toxin A Subunit from the Golgi Involves COPI, p23, and the COOH Terminus of Erd2p. JCB 143: 601-612 [Abstract] [Full Text]
Harter, C., Wieland, F. T. (1998). A single binding site for dilysine retrieval motifs and p23 within the gamma �subunit of coatomer. Proc. Natl. Acad. Sci. USA 95: 11649-11654 [Abstract] [Full Text]
Helms, J. B., Helms-Brons, D., Brugger, B., Gkantiragas, I., Eberle, H., Nickel, W., Nurnberg, B., Gerdes, H.-H., Wieland, F. T. (1998). A Putative Heterotrimeric G Protein Inhibits the Fusion of COPI-coated Vesicles. SEGREGATION OF HETEROTRIMERIC G PROTEINS FROM COPI-COATED VESICLES. J. Biol. Chem. 273: 15203-15208 [Abstract] [Full Text]
Passreiter, M., Anton, M., Lay, D., Frank, R., Harter, C., Wieland, F. T., Gorgas, K., Just, W. W. (1998). Peroxisome Biogenesis: Involvement of ARF and Coatomer. JCB 141: 373-383 [Abstract] [Full Text]
Pavel, J., Harter, C., Wieland, F. T. (1998). Reversible dissociation of coatomer: Functional characterization of a beta /delta -coat protein�subcomplex. Proc. Natl. Acad. Sci. USA 95: 2140-2145 [Abstract] [Full Text]
Hong, W (1998). Protein transport from the endoplasmic reticulum to the Golgi apparatus. J. Cell Sci. 111: 2831-2839 [Abstract]
Nickel, W, Malsam, J, Gorgas, K, Ravazzola, M, Jenne, N, Helms, J., Wieland, F. (1998). Uptake by COPI-coated vesicles of both anterograde and retrograde cargo is inhibited by GTPgammaS in vitro. J. Cell Sci. 111: 3081-3090 [Abstract]
Pepperkok, R, Lowe, M, Burke, B, Kreis, T. (1998). Three distinct steps in transport of vesicular stomatitis virus glycoprotein from the ER to the cell surface in vivo with differential sensitivities to GTP gamma S. J. Cell Sci. 111: 1877-1888 [Abstract]
Schweizer, A., Kornfeld, S., Rohrer, J. (1997). Proper sorting of the cation-dependent mannose 6-phosphate receptor in endosomes depends on a pair of aromatic amino acids in its�cytoplasmic�tail. Proc. Natl. Acad. Sci. USA 94: 14471-14476 [Abstract] [Full Text]
Kappeler, F., Klopfenstein, D. R.�Ch., Foguet, M., Paccaud, J.-P., Hauri, H.-P. (1997). The Recycling of ERGIC-53 in the Early Secretory Pathway. ERGIC-53 CARRIES A CYTOSOLIC ENDOPLASMIC RETICULUM-EXIT DETERMINANT INTERACTING WITH COPII. J. Biol. Chem. 272: 31801-31808 [Abstract] [Full Text]
Rojo, M., Pepperkok, R., Emery, G., Kellner, R., Stang, E., Parton, R. G., Gruenberg, J. (1997). Involvement of the Transmembrane Protein p23 in Biosynthetic Protein Transport. JCB 139: 1119-1135 [Abstract] [Full Text]
Gu, F., Aniento, F., Parton, R. G., Gruenberg, J. (1997). Functional Dissection of COP-I Subunits in the Biogenesis of Multivesicular Endosomes. JCB 139: 1183-1195 [Abstract] [Full Text]
Nickel, W., Sohn, K., Bunning, C., Wieland, F. T. (1997). p23, a major COPI-vesicle membrane protein, constitutively cycles through the early secretory�pathway. Proc. Natl. Acad. Sci. USA 94: 11393-11398 [Abstract] [Full Text]
Fiedler, K., Rothman, J. E. (1997). Sorting Determinants in the Transmembrane Domain of p24 Proteins. J. Biol. Chem. 272: 24739-24742 [Abstract] [Full Text]
Hudson, R. T., Draper, R. K. (1997). Interaction of Coatomer with Aminoglycoside Antibiotics: Evidence That Coatomer Has at Least Two Dilysine Binding Sites. Mol. Biol. Cell 8: 1901-1910 [Abstract] [Full Text]
Zhao, L., Helms, J. B., Brugger, B., Harter, C., Martoglio, B., Graf, R., Brunner, J., Wieland, F. T. (1997). Direct and GTP-dependent interaction of ADP ribosylation factor 1�with coatomer subunit�beta. Proc. Natl. Acad. Sci. USA 94: 4418-4423 [Abstract] [Full Text]
Gomez, M., Scales, S. J., Kreis, T. E., Perez, F. (2000). Membrane Recruitment of Coatomer and Binding to Dilysine Signals Are Separate Events. J. Biol. Chem. 275: 29162-29169 [Abstract] [Full Text]
Lanoix, J., Ouwendijk, J., Stark, A., Szafer, E., Cassel, D., Dejgaard, K., Weiss, M., Nilsson, T. (2001). Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1. JCB 155: 1199-1212 [Abstract] [Full Text]
Fucini, R. V., Chen, J.-L., Sharma, C., Kessels, M. M., Stamnes, M. (2002). Golgi Vesicle Proteins Are Linked to the Assembly of an Actin Complex Defined by mAbp1. Mol. Biol. Cell 13: 621-631 [Abstract] [Full Text]