Docking of Yeast Vacuoles Is Catalyzed by the Ras-like GTPase Ypt7p after Symmetric Priming by Sec18p (NSF)

doi:10.1083/jcb.136.2.307

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© The Rockefeller University Press, 0021-9525/1997//307 $5.00
The Journal of Cell Biology, Volume 136, Number 2, , 1997 307-317

Article

Docking of Yeast Vacuoles Is Catalyzed by the Ras-like GTPase Ypt7p after Symmetric Priming by Sec18p (NSF)

Andreas Mayer and William Wickner

Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755-3844

Vacuole inheritance in yeast involves the formation of tubularand vesicular "segregation structures" which migrate into thebud and fuse there to establish the daughter cell vacuole. Vacuolefusion has been reconstituted in vitro and may be used as amodel for an NSF-dependent reaction of priming, docking, andfusion. We have developed biochemical and microscopic assaysfor the docking step of in vitro vacuole fusion and characterizedits requirements. The vacuoles must be primed for docking bythe action of Sec17p ( ${alpha}$ -SNAP) and Sec18p (NSF). Priming is necessaryfor both fusion partners. It produces a labile state whichrequires rapid docking in order to lead productively to fusion.In addition to Sec17p/Sec18p, docking requires the activityof the Ras-like GTPase Ypt7p. Unlike Sec17p/Sec18p, which mustact before docking, Ypt7p is directly involved in the dockingprocess itself.

Abbreviations used in this paper: NSF, NEN sensitive fusion protein; SNAP, soluble NSF attachment protein; SNARE, SNAP receptor.

The work was supported by a grant from the National Institutesof General Medical Sciences.

Please address all correspondence to W. Wickner, Departmentof Biochemistry, Dartmouth Medical School, 7200 Vail Building,Hanover, NH 03755-3844. Tel.: (603) 650-1701. Fax: (603) 650-1353.

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