© The Rockefeller University Press,
0021-9525/1997//487 $5.00
The Journal of Cell Biology, Volume 136, Number 3,
, 1997 487-500
A Centromere DNA-binding Protein from Fission Yeast Affects Chromosome Segregation and Has Homology to Human CENP-B
Dana Halverson,
Mary Baum,
Janet Stryker,
John Carbon, and
Louise Clarke
Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106
Genetic and biochemical strategies have been used to identify Schizosaccharomyces pombe proteins with roles in centromere function. One protein, identified by both approaches, shows significant homology to the human centromere DNA-binding protein, CENP-B, and is identical to Abp1p (autonomously replicating sequence-binding protein 1) (Murakami, Y., J.A. Huberman, and J. Hurwitz. 1996. Proc. Natl. Acad. Sci. USA. 93:502–507). Abp1p binds in vitro specifically to at least three sites in centromeric central core DNA of S. pombe chromosome II (cc2). Overexpression of abp1 affects mitotic chromosome stability in S. pombe. Although inactivation of the abp1 gene is not lethal, the abp1 null strain displays marked mitotic chromosome instability and a pronounced meiotic defect. The identification of a CENP-B–related centromere DNA-binding protein in S. pombe strongly supports the hypothesis that fission yeast centromeres are structurally and functionally related to the centromeres of higher eukaryotes.
Abbreviations used in this paper: Abp1p, autonomously replicating sequence-binding protein; ARS, autonomously replicating sequence; cc2, central core DNA of Schizosaccharomyces pombe chromosome II; DAPI, 4', 6-Diamidino-2-phenylindole; IS, insertion sequence.
Please address all correspondence to Louise Clark, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106. Tel.: (805) 893-3624. Fax: (805) 893-4724. e-mail: clarke{at}lifesci.lscf.ucsb.edu

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