Mdm12p, a Component Required for Mitochondrial Inheritance That Is Conserved between Budding and Fission Yeast

doi:10.1083/jcb.136.3.545

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© The Rockefeller University Press, 0021-9525/1997//545 $5.00
The Journal of Cell Biology, Volume 136, Number 3, , 1997 545-553

Article

Mdm12p, a Component Required for Mitochondrial Inheritance That Is Conserved between Budding and Fission Yeast

Karen H. Berger, L. Farah Sogo, and Michael P. Yaffe

Department of Biology, University of California, San Diego, La Jolla, California 92093-0347

Saccharomyces cerevisiae cells lacking the MDM12 gene productdisplay temperature-sensitive growth and possess abnormallylarge, round mitochondria that are defective for inheritanceby daughter buds. Analysis of the wild-type MDM12 gene revealedits product to be a 31-kD polypeptide that is homologous toa protein of the fission yeast Schizosaccharomyces pombe. Whenexpressed in S. cerevisiae, the S. pombe Mdm12p homolog conferreda dominant-negative phenotype of giant mitochondria and aberrantmitochondrial distribution, suggesting partial functional conservationof Mdm12p activity between budding and fission yeast. The S.cerevisiae Mdm12p was localized by indirect immunofluorescencemicroscopy and by subcellular fractionation and immunodetectionto the mitochondrial outer membrane and displayed biochemical properties of an integral membrane protein. Mdm12p is thethird mitochondrial outer membrane protein required for normalmitochondrial morphology and distribution to be identified inS. cerevisiae and the first such mitochondrial component thatis conserved between two different species.

Abbreviations used in this paper: DAPI, 4,6-diamidino-2-phenylindole; DASPMI, 2-(4-dimethylaminostryl)-1-methylpyridinium iodide; mdm, mitochondrial distribution and morphology; YPD, yeast extract/peptone/ glucose; YPG, yeast extract/peptone/glycerol.

Please address all correspondence to Michael P. Yaffe, Departmentof Biology, 0347, University of California, San Diego, La Jolla,CA 92093-0347. Tel.: (619) 534-4769. Fax: (619) 534-4403. e-mail:myaffe{at}ucsd.edu

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