JCB logo
R&D Systems: New Poster Available
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1223K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Belgareh, N.
Right arrow Articles by Doye, V.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Belgareh, N.
Right arrow Articles by Doye, V.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/1997//747 $5.00
The Journal of Cell Biology, Volume 136, Number 4, , 1997 747-759

Article

Dynamics of Nuclear Pore Distribution in Nucleoporin Mutant Yeast Cells



Naïma Belgareh and Valérie Doye

Centre National de la Recherche Scientifique (CNRS) UMR144, Institut Curie, 75231 Paris Cedex 05, France

To follow the dynamics of nuclear pore distribution in living yeast cells, we have generated fusion proteins between the green fluorescent protein (GFP) and the yeast nucleoporins Nup49p and Nup133p. In nup133– dividing cells that display a constitutive nuclear pore clustering, in vivo analysis of GFP-Nup49p localization revealed changes in the distribution of nuclear pore complex (NPC) clusters. Furthermore, upon induction of Nup133p expression in a GAL-nup133 strain, a progressive fragmentation of the NPC aggregates was observed that in turn led to a wild-type nuclear pore distribution. To try to uncouple Nup133p- induced NPC redistribution from successive nuclear divisions and nuclear pore biogenesis, we devised an assay based on the formation of heterokaryons between nup133– mutants and cells either expressing or overexpressing Nup133p. Under these conditions, the use of GFP-Nup133p and GFP-Nup49p fusion proteins revealed that Nup133p can be rapidly targeted to the clustered nuclear pores, where its amino-terminal domain is required to promote the redistribution of preexisting NPCs.

Abbreviations used in this paper: DAPI, 4',6'-diamindino-2-phenylindole; GFP, green fluorescent protein; MD, megadaltons; NPC, nuclear pore complex; SDC or SGalC, synthetic dextrose or galactose complete media; YPDA or YPGalA, yeast extract, bactopeptone, dextrose or galactose, and adenine.

Address all correspondence to Valérie Doye, CNRS UMR144, Institut Curie, 26, rue d'Ulm, 75231 Paris Cedex 05, France. Tel.: (33) 1 42 34 64 13. Fax: (33) 1 42 34 64 21. E-mail: Valerie.Doye{at}curie.fr


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents