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© The Rockefeller University Press, 0021-9525/1997//761 $5.00
The Journal of Cell Biology, Volume 136, Number 4, , 1997 761-773

Article

A Mouse Cytoplasmic Exoribonuclease (mXRN1p) with Preference for G4 Tetraplex Substrates



Vladimir I. Bashkirov*,||, Harry Scherthan{ddagger}, Jachen A. Solinger*, Jean-Marie Buerstedde§, and Wolf-Dietrich Heyer*

* Institute of General Microbiology, University of Bern, CH-3012 Bern, Switzerland; {ddagger} Section of Human Biology and Genetics, University of Kaiserslautern, D-67653 Kaiserslautern, Germany; § Basel Institute of Immunology, CH-4005 Basel, Switzerland; and || Institute of Gene Biology, Moscow 117 334, Russia

Exoribonucleases are important enzymes for the turnover of cellular RNA species. We have isolated the first mammalian cDNA from mouse demonstrated to encode a 5'–3' exoribonuclease. The structural conservation of the predicted protein and complementation data in Saccharomyces cerevisiae suggest a role in cytoplasmic mRNA turnover and pre-rRNA processing similar to that of the major cytoplasmic exoribonuclease Xrn1p in yeast. Therefore, a key component of the mRNA decay system in S. cerevisiae has been conserved in evolution from yeasts to mammals. The purified mouse protein (mXRN1p) exhibited a novel substrate preference for G4 RNA tetraplex–containing substrates demonstrated in binding and hydrolysis experiments. mXRN1p is the first RNA turnover function that has been localized in the cytoplasm of mammalian cells. mXRN1p was distributed in small granules and was highly enriched in discrete, prominent foci. The specificity of mXRN1p suggests that RNAs containing G4 tetraplex structures may occur in vivo and may have a role in RNA turnover.

Please address all correspondence to Wolf-Dietrich Heyer, Institute of General Microbiology, Baltzer-Str. 4, CH-3012 Bern, Switzerland. Tel.: 41 31 631 46 56. Fax: 41 31 631 46 84. e-mail: heyer{at}imb.unibe.ch


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