© The Rockefeller University Press,
0021-9525/1997//833 $5.00
The Journal of Cell Biology, Volume 136, Number 4,
, 1997 833-844
Mitochondrial Participation in the Intracellular Ca2+ Network
Donner F. Babcock,
James Herrington,
Paul C. Goodwin*,
Young Bae Park, and
Bertil Hille
Department of Physiology & Biophysics, University of Washington, Seattle, Washington 98195-7290; and * Image Analysis Laboratory, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Calcium can activate mitochondrial metabolism, and the possibility that mitochondrial Ca2+ uptake and extrusion modulate free cytosolic [Ca2+] (Cac) now has renewed interest. We use whole-cell and perforated patch clamp methods together with rapid local perfusion to introduce probes and inhibitors to rat chromaffin cells, to evoke Ca2+ entry, and to monitor Ca2+-activated currents that report near-surface [Ca2+]. We show that rapid recovery from elevations of Cac requires both the mitochondrial Ca2+ uniporter and the mitochondrial energization that drives Ca2+ uptake through it. Applying imaging and single-cell photometric methods, we find that the probe rhod-2 selectively localizes to mitochondria and uses its responses to quantify mitochondrial free [Ca2+] (Cam). The indicated resting Cam of 100–200 nM is similar to the resting Cac reported by the probes indo-1 and Calcium Green, or its dextran conjugate in the cytoplasm. Simultaneous monitoring of Cam and Cac at high temporal resolution shows that, although Cam increases less than Cac, mitochondrial sequestration of Ca2+ is fast and has high capacity. We find that mitochondrial Ca2+ uptake limits the rise and underlies the rapid decay of Cac excursions produced by Ca2+ entry or by mobilization of reticular stores. We also find that subsequent export of Ca2+ from mitochondria, seen as declining Cam, prolongs complete Cac recovery and that suppressing export of Ca2+, by inhibition of the mitochondrial Na+/ Ca2+ exchanger, reversibly hastens final recovery of Cac. We conclude that mitochondria are active participants in cellular Ca2+ signaling, whose unique role is determined by their ability to rapidly accumulate and then release large quantities of Ca2+.
Abbreviations used in this paper: Cac, free cytosolic [Ca2+]; Cam, free intramitochondrial [Ca2+]; CGP-37157, 7-chloro-3,5-dihydro-5-phenyl-1H4,1-benzothiazepine-2-on; BHQ, 2,5 di(t-butyl)-1,4-hydroxyquinone; CCCP, carbonyl cyanide m-chlorophenylhydrazone.
Please address all correspondence to B. Hille, Department of Physiology & Biophysics, G424 Health Sciences Building, University of Washington, Box 357290, Seattle, WA 98195-7290. Tel.: (206) 543-8639. Fax: (206) 6850619. E-Mail: hille{at}u.washington.edu

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[Full Text]
-
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(2005). Role of Mitochondrial Na+ Concentration, Measured by CoroNa Red, in the Protection of Metabolically Inhibited MDCK Cells. J. Am. Soc. Nephrol.
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-
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-
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-
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-
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[Abstract]
[Full Text]
-
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287: H2070-H2077
[Abstract]
[Full Text]
-
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(2004). Na-K-Cl Cotransporter-Mediated Intracellular Na+ Accumulation Affects Ca2+ Signaling in Astrocytes in an In Vitro Ischemic Model. J. Neurosci.
24: 9585-9597
[Abstract]
[Full Text]
-
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(2004). Mitochondrial calcium sequestration and protein kinase C cooperate in the regulation of cortical F-actin disassembly and secretion in bovine chromaffin cells. J. Physiol.
560: 63-76
[Abstract]
[Full Text]
-
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[Abstract]
[Full Text]
-
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[Abstract]
[Full Text]
-
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(2004). Vanilloid Receptor 1 Regulates Multiple Calcium Compartments and Contributes to Ca2+-induced Ca2+ Release in Sensory Neurons. J. Biol. Chem.
279: 16377-16387
[Abstract]
[Full Text]
-
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[Abstract]
[Full Text]
-
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(2004). Ca2+ uptake in mitochondria occurs via the reverse action of the Na+/Ca2+ exchanger in metabolically inhibited MDCK cells. Am. J. Physiol. Renal Physiol.
286: F784-F794
[Abstract]
[Full Text]
-
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[Abstract]
[Full Text]
-
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2004: re1-re1
[Abstract]
[Full Text]
-
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18: 252-256
[Abstract]
[Full Text]
-
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(2003). Sustained Ca2+ Transfer across Mitochondria Is Essential for Mitochondrial Ca2+ Buffering, Store-operated Ca2+ Entry, and Ca2+ Store Refilling. J. Biol. Chem.
278: 44769-44779
[Abstract]
[Full Text]
-
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18: 2429-2440
[Abstract]
[Full Text]
-
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552: 393-402
[Abstract]
[Full Text]
-
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278: 38686-38692
[Abstract]
[Full Text]
-
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122: 377-387
[Abstract]
[Full Text]
-
Bruton, J., Tavi, P., Aydin, J., Westerblad, H., Lannergren, J.
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551: 179-190
[Abstract]
[Full Text]
-
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(2003). Metabotropic Receptor-Mediated Ca2+ Signaling Elevates Mitochondrial Ca2+ and Stimulates Oxidative Metabolism in Hippocampal Slice Cultures. J. Neurophysiol.
90: 613-621
[Abstract]
[Full Text]
-
Talbot, J. D., David, G., Barrett, E. F.
(2003). Inhibition of Mitochondrial Ca2+ Uptake Affects Phasic Release From Motor Terminals Differently Depending on External [Ca2+]. J. Neurophysiol.
90: 491-502
[Abstract]
[Full Text]
-
Wennemuth, G., Babcock, D. F., Hille, B.
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122: 115-128
[Abstract]
[Full Text]
-
Ho, H.-C., Suarez, S. S.
(2003). Characterization of the Intracellular Calcium Store at the Base of the Sperm Flagellum That Regulates Hyperactivated Motility. Biol. Reprod.
68: 1590-1596
[Abstract]
[Full Text]
-
Jambrina, E., Alonso, R., Alcalde, M., del Carmen Rodriguez, M., Serrano, A., Martinez-A., C., Garcia-Sancho, J., Izquierdo, M.
(2003). Calcium Influx through Receptor-operated Channel Induces Mitochondria-triggered Paraptotic Cell Death. J. Biol. Chem.
278: 14134-14145
[Abstract]
[Full Text]
-
Ladewig, T., Kloppenburg, P., Lalley, P. M, Zipfel, W. R, Webb, W. W, Keller, B. U
(2003). Spatial profiles of store-dependent calcium release in motoneurones of the nucleus hypoglossus from newborn mouse. J. Physiol.
547: 775-787
[Abstract]
[Full Text]
-
Krizaj, D., Anthony Lai, F, Copenhagen, D. R
(2003). Ryanodine stores and calcium regulation in the inner segments of salamander rods and cones. J. Physiol.
547: 761-774
[Abstract]
[Full Text]
-
Parekh, A. B
(2003). Store-operated Ca2+ entry: dynamic interplay between endoplasmic reticulum, mitochondria and plasma membrane. J. Physiol.
547: 333-348
[Abstract]
[Full Text]
-
Wang, Y.-X., Zheng, Y.-M., Abdullaev, I., Kotlikoff, M. I.
(2003). Metabolic inhibition with cyanide induces calcium release in pulmonary artery myocytes and Xenopus oocytes. Am. J. Physiol. Cell Physiol.
284: C378-C388
[Abstract]
[Full Text]
-
SZADO, T., KUO, K.-H., BERNARD-HELARY, K., POBURKO, D., LEE, C. H., SEOW, C., RUEGG, U. T., VAN BREEMEN, C.
(2003). Agonist-induced mitochondrial Ca2+ transients in smooth muscle. FASEB J.
17: 28-37
[Abstract]
[Full Text]
-
Pivovarova, N. B., Pozzo-Miller, L. D., Hongpaisan, J., Andrews, S. B.
(2002). Correlated Calcium Uptake and Release by Mitochondria and Endoplasmic Reticulum of CA3 Hippocampal Dendrites after Afferent Synaptic Stimulation. J. Neurosci.
22: 10653-10661
[Abstract]
[Full Text]
-
Parthasarathi, K., Ichimura, H., Quadri, S., Issekutz, A., Bhattacharya, J.
(2002). Mitochondrial Reactive Oxygen Species Regulate Spatial Profile of Proinflammatory Responses in Lung Venular Capillaries. J. Immunol.
169: 7078-7086
[Abstract]
[Full Text]
-
Kovacs, R., Schuchmann, S., Gabriel, S., Kann, O., Kardos, J., Heinemann, U.
(2002). Free Radical-Mediated Cell Damage After Experimental Status Epilepticus in Hippocampal Slice Cultures. J. Neurophysiol.
88: 2909-2918
[Abstract]
[Full Text]
-
Kamishima, T., Quayle, J. M.
(2002). Mitochondrial Ca2+ uptake is important over low [Ca2+]i range in arterial smooth muscle. Am. J. Physiol. Heart Circ. Physiol.
283: H2431-H2439
[Abstract]
[Full Text]
-
Rapizzi, E., Pinton, P., Szabadkai, G., Wieckowski, M. R., Vandecasteele, G., Baird, G., Tuft, R. A., Fogarty, K. E., Rizzuto, R.
(2002). Recombinant expression of the voltage-dependent anion channel enhances the transfer of Ca2+ microdomains to mitochondria. JCB
159: 613-624
[Abstract]
[Full Text]
-
Arnaudeau, S., Frieden, M., Nakamura, K., Castelbou, C., Michalak, M., Demaurex, N.
(2002). Calreticulin Differentially Modulates Calcium Uptake and Release in the Endoplasmic Reticulum and Mitochondria. J. Biol. Chem.
277: 46696-46705
[Abstract]
[Full Text]
-
Billups, B., Forsythe, I. D.
(2002). Presynaptic Mitochondrial Calcium Sequestration Influences Transmission at Mammalian Central Synapses. J. Neurosci.
22: 5840-5847
[Abstract]
[Full Text]
-
Berke, B., Wu, C.-F.
(2002). Regional Calcium Regulation within Cultured Drosophila Neurons: Effects of Altered cAMP Metabolism by the Learning Mutations dunce and rutabaga. J. Neurosci.
22: 4437-4447
[Abstract]
[Full Text]
-
Medler, K., Gleason, E. L.
(2002). Mitochondrial Ca2+ Buffering Regulates Synaptic Transmission Between Retinal Amacrine Cells. J. Neurophysiol.
87: 1426-1439
[Abstract]
[Full Text]
-
VILLALOBOS, C., NUNEZ, L., MONTERO, M., GARCIA, A. G., ALONSO, M. T., CHAMERO, P., ALVAREZ, J., GARCIA-SANCHO, J.
(2002). Redistribution of Ca2+ among cytosol and organella during stimulation of bovine chromaffin cells. FASEB J.
16: 343-353
[Abstract]
[Full Text]
-
Voronina, S, Sukhomlin, T, Johnson, P R, Erdemli, G, Petersen, O H, Tepikin, A
(2002). Correlation of NADH and Ca2+ signals in mouse pancreatic acinar cells. J. Physiol.
539: 41-52
[Abstract]
[Full Text]
-
Van Blerkom, J., Davis, P., Mathwig, V., Alexander, S.
(2002). Domains of high-polarized and low-polarized mitochondria may occur in mouse and human oocytes and early embryos. Hum Reprod
17: 393-406
[Abstract]
[Full Text]
-
Wang, Y., Prpic, V., Green, G. M., Reeve, J. R. Jr., Liddle, R. A.
(2002). Luminal CCK-releasing factor stimulates CCK release from human intestinal endocrine and STC-1 cells. Am. J. Physiol. Gastrointest. Liver Physiol.
282: G16-G22
[Abstract]
[Full Text]
-
Woollacott, A. J., Simpson, P. B.
(2001). High Throughput Fluorescence Assays for the Measurement of Mitochondrial Activity in Intact Human Neuroblastoma Cells. J Biomol Screen
6: 413-420
[Abstract]
-
Sanchez, J. A, Garcia, M. C, Sharma, V. K, Young, K. C, Matlib, M. A, Sheu, S.-S.
(2001). Mitochondria regulate inactivation of L-type Ca2+ channels in rat heart. J. Physiol.
536: 387-396
[Abstract]
[Full Text]
-
Elimadi, A., Haddad, P. S.
(2001). Cold preservation-warm reoxygenation increases hepatocyte steady-state Ca2+ and response to Ca2+-mobilizing agonist. Am. J. Physiol. Gastrointest. Liver Physiol.
281: G809-G815
[Abstract]
[Full Text]
-
Barrett, E. F.
(2001). Contrasting Contributions of Endoplasmic Reticulum and Mitochondria to Ca2+ Handling in Neurons. JGP
118: 79-82
[Full Text]
-
Hernandez-Guijo, J. M., Maneu-Flores, V. E., Ruiz-Nuno, A., Villarroya, M., Garcia, A. G., Gandia, L.
(2001). Calcium-Dependent Inhibition of L, N, and P/Q Ca2+ Channels in Chromaffin Cells: Role of Mitochondria. J. Neurosci.
21: 2553-2560
[Abstract]
[Full Text]
-
Calupca, M. A, Prior, C., Merriam, L. A, Hendricks, G. M, Parsons, R. L
(2001). Presynaptic function is altered in snake K+-depolarized motor nerve terminals containing compromised mitochondria. J. Physiol.
532: 217-227
[Abstract]
[Full Text]
-
Nagai, T., Sawano, A., Park, E. S., Miyawaki, A.
(2001). Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. USA
10.1073/pnas.051636098v1
[Abstract]
[Full Text]
-
Wang, S., Cone, J., Liu, Y.
(2001). Dual roles of mitochondrial KATP channels in diazoxide-mediated protection in isolated rabbit hearts. Am. J. Physiol. Heart Circ. Physiol.
280: H246-H255
[Abstract]
[Full Text]
-
Montero, M., Alonso, M. T., Albillos, A., García-Sancho, J., Alvarez, J.
(2001). Mitochondrial Ca2+-induced Ca2+ Release Mediated by the Ca2+ Uniporter. Mol. Biol. Cell
12: 63-71
[Abstract]
[Full Text]
-
Rowland, K. C., Irby, N. K., Spirou, G. A.
(2000). Specialized Synapse-Associated Structures within the Calyx of Held. J. Neurosci.
20: 9135-9144
[Abstract]
[Full Text]
-
Pacher, P., Csordas, G., Schneider, T. G, Hajnoczky, G.
(2000). Quantification of calcium signal transmission from sarco-endoplasmic reticulum to the mitochondria. J. Physiol.
529: 553-564
[Abstract]
[Full Text]
-
Rizzuto, R., Bernardi, P., Pozzan, T.
(2000). Mitochondria as all-round players of the calcium game. J. Physiol.
529: 37-47
[Abstract]
[Full Text]
-
Duchen, M. R
(2000). Mitochondria and calcium: from cell signalling to cell death. J. Physiol.
529: 57-68
[Abstract]
[Full Text]
-
Hajnoczky, G., Csordas, G., Madesh, M., Pacher, P.
(2000). The machinery of local Ca2+ signalling between sarco-endoplasmic reticulum and mitochondria. J. Physiol.
529: 69-81
[Abstract]
[Full Text]
-
David, G., Barrett, E. F.
(2000). Stimulation-Evoked Increases in Cytosolic [Ca2+] in Mouse Motor Nerve Terminals Are Limited by Mitochondrial Uptake and Are Temperature-Dependent. J. Neurosci.
20: 7290-7296
[Abstract]
[Full Text]
-
Zimmermann, B.
(2000). Control of InsP3-induced Ca2+ oscillations in permeabilized blowfly salivary gland cells: contribution of mitochondria. J. Physiol.
525: 707-719
[Abstract]
[Full Text]
-
Pozzo-Miller, L. D, Connor, J. A, Andrews, S B.
(2000). Microheterogeneity of calcium signalling in dendrites. J. Physiol.
525: 53-61
[Abstract]
[Full Text]
-
Quesada, I., Martin, F., Soria, B.
(2000). Nutrient modulation of polarized and sustained submembrane Ca2+ microgradients in mouse pancreatic islet cells. J. Physiol.
525: 159-167
[Abstract]
[Full Text]
-
Szalai, G., Csordas, G., Hantash, B. M., Thomas, A. P., Hajnoczky, G.
(2000). Calcium Signal Transmission between Ryanodine Receptors and Mitochondria. J. Biol. Chem.
275: 15305-15313
[Abstract]
[Full Text]
-
Jaconi, M., Bony, C., Richards, S. M., Terzic, A., Arnaudeau, S., Vassort, G., Pucéat, M.
(2000). Inositol 1,4,5-Trisphosphate Directs Ca2+ Flow between Mitochondria and the Endoplasmic/Sarcoplasmic Reticulum: A Role in Regulating Cardiac Autonomic Ca2+ Spiking. Mol. Biol. Cell
11: 1845-1858
[Abstract]
[Full Text]
-
Schuchmann, S., Luckermann, M., Kulik, A., Heinemann, U., Ballanyi, K.
(2000). Ca2+- and Metabolism-Related Changes of Mitochondrial Potential in Voltage-Clamped CA1 Pyramidal Neurons In Situ. J. Neurophysiol.
83: 1710-1721
[Abstract]
[Full Text]
-
Colegrove, S. L., Albrecht, M. A., Friel, D. D.
(2000). Dissection of Mitochondrial Ca2+ Uptake and Release Fluxes in Situ after Depolarization-Evoked [Ca2+]i Elevations in Sympathetic Neurons. JGP
115: 351-370
[Abstract]
[Full Text]
-
Colegrove, S. L., Albrecht, M. A., Friel, D. D.
(2000). Quantitative Analysis of Mitochondrial Ca2+ Uptake and Release Pathways in Sympathetic Neurons: Reconstruction of the Recovery after Depolarization-Evoked [Ca2+]i Elevations. JGP
115: 371-388
[Abstract]
[Full Text]
-
Drummond, R. M, Mix, T C. H, Tuft, R. A, Walsh, J. V Jr, Fay, F. S
(2000). Mitochondrial Ca2+ homeostasis during Ca2+ influx and Ca2+ release in gastric myocytes from Bufo marinus. J. Physiol.
522: 375-390
[Abstract]
[Full Text]
-
Carriedo, S. G., Sensi, S. L., Yin, H. Z., Weiss, J. H.
(2000). AMPA Exposures Induce Mitochondrial Ca2+ Overload and ROS Generation in Spinal Motor Neurons In Vitro. J. Neurosci.
20: 240-250
[Abstract]
[Full Text]
-
Nicholls, D. G., Budd, S. L.
(2000). Mitochondria and Neuronal Survival. Physiol. Rev.
80: 315-360
[Abstract]
[Full Text]
-
Jouaville, L. S., Pinton, P., Bastianutto, C., Rutter, G. A., Rizzuto, R.
(1999). Regulation of mitochondrial ATP synthesis by calcium: Evidence for a long-term metabolic priming. Proc. Natl. Acad. Sci. USA
96: 13807-13812
[Abstract]
[Full Text]
-
Zhu, L., Ling, S., Yu, X.-D., Venkatesh, L. K., Subramanian, T., Chinnadurai, G., Kuo, T. H.
(1999). Modulation of Mitochondrial Ca2+ Homeostasis by Bcl-2. J. Biol. Chem.
274: 33267-33273
[Abstract]
[Full Text]
-
Jonas, E. A., Buchanan, J., Kaczmarek, L. K.
(1999). Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission. Science
286: 1347-1350
[Abstract]
[Full Text]
-
Giovannucci, D. R., Hlubek, M. D., Stuenkel, E. L.
(1999). Mitochondria Regulate the Ca2+-Exocytosis Relationship of Bovine Adrenal Chromaffin Cells. J. Neurosci.
19: 9261-9270
[Abstract]
[Full Text]
-
Takahashi, A., Camacho, P., Lechleiter, J. D., Herman, B.
(1999). Measurement of Intracellular Calcium. Physiol. Rev.
79: 1089-1125
[Abstract]
[Full Text]
-
Bernardi, P.
(1999). Mitochondrial Transport of Cations: Channels, Exchangers, and Permeability Transition. Physiol. Rev.
79: 1127-1155
[Abstract]
[Full Text]
-
David, G.
(1999). Mitochondrial Clearance of Cytosolic Ca2+ in Stimulated Lizard Motor Nerve Terminals Proceeds without Progressive Elevation of Mitochondrial Matrix [Ca2+]. J. Neurosci.
19: 7495-7506
[Abstract]
[Full Text]
-
Mak, D.-O. D., McBride, S., Foskett, J. K.
(1999). ATP Regulation of Type 1 Inositol 1,4,5-Trisphosphate Receptor Channel Gating by Allosteric Tuning of Ca2+ Activation. J. Biol. Chem.
274: 22231-22237
[Abstract]
[Full Text]
-
Pivovarova, N. B., Hongpaisan, J., Andrews, S. B., Friel, D. D.
(1999). Depolarization-Induced Mitochondrial Ca Accumulation in Sympathetic Neurons: Spatial and Temporal Characteristics. J. Neurosci.
19: 6372-6384
[Abstract]
[Full Text]