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© The Rockefeller University Press, 0021-9525/1997//895 $5.00
The Journal of Cell Biology, Volume 136, Number 4, , 1997 895-906

Article

Chicken Acidic Leucine-rich EGF-like Domain Containing Brain Protein (CALEB), a Neural Member of the EGF Family of Differentiation Factors, Is Implicated in Neurite Formation



Stefan Schumacher*, Hansjürgen Volkmer*, Fritz Buck{ddagger}, Albrecht Otto*, Attila Tárnok§, Siegfried Roth, and Fritz G. Rathjen*

* Max-Delbrück-Centrum für Molekulare Medizin, D-13122 Berlin, Germany; {ddagger} Institut für Zellbiochemie, D-20246 Hamburg, Germany; and § Herzzentrum Leipzig GmbH, D-04289 Leipzig, Germany

Chicken acidic leucine-rich EGF-like domain containing brain protein (CALEB) was identified by combining binding assays with immunological screens in the chicken nervous system as a novel member of the EGF family of differentiation factors. cDNA cloning indicates that CALEB is a multidomain protein that consists of an NH2-terminal glycosylation region, a leucine-proline–rich segment, an acidic box, a single EGF-like domain, a transmembrane, and a short cytoplasmic stretch. In the developing nervous system, CALEB is associated with glial and neuronal surfaces. CALEB is composed of a 140/130-kD doublet, an 80-kD band, and a chondroitinsulfate-containing 200-kD component. The latter two components are expressed in the embryonic nervous system and are downregulated in the adult nervous system. CALEB binds to the extracellular matrix glycoproteins tenascin-C and -R. In vitro antibody perturbation experiments reveal a participation of CALEB in neurite formation in a permissive environment.

Abbreviations used in this paper: aa, amino acid; CALEB, chicken acidic leucine-rich EGF-like domain containing brain protein; E, embryonic day; ECM, extracellular matrix; IgSF, immunoglobulin superfamily; IPL and OPL, inner and outer plexiform layer; NCAM, neural cell adhesion molecule; NgCAM, neuron-glia cell adhesion molecule; NrCAM, N gCAM-related cell adhesion molecule; OFL, optic fiber layer; TN-C and -R, tenascin-C and -R.

Address all correspondence to Fritz G. Rathjen, Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Str. 10, D-13122 Berlin, Germany.


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