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* Howard Hughes Medical Institute, Division of Cellular and Molecular Medicine, Department of Pharmacology, University of
California at San Diego, La Jolla, California 92093-0683; The kinesin superfamily is a large group of
proteins (kinesin-like proteins [KLPs]) that share sequence similarity with the microtubule (MT) motor kinesin. Several members of this superfamily have been
implicated in various stages of mitosis and meiosis. Here we report our studies on KLP67A of Drosophila.
DNA sequence analysis of KLP67A predicts an MT
motor protein with an amino-terminal motor domain.
To prove this directly, KLP67A expressed in Escherichia coli was shown in an in vitro motility assay to move
MTs in the plus direction. We also report expression
analyses at both the mRNA and protein level, which
implicate KLP67A in the localization of mitochondria
in undifferentiated cell types. In situ hybridization studies of the KLP67A mRNA during embryogenesis and
larval central nervous system development indicate a
proliferation-specific expression pattern. Furthermore,
when affinity-purified anti-KLP67A antisera are used
to stain blastoderm embryos, mitochondria in the region of the spindle asters are labeled. These data suggest that KLP67A is a mitotic motor of Drosophila that
may have the unique role of positioning mitochondria
near the spindle.
Program in Molecular Medicine, University of Massachusetts Medical
Center, Worcester, Massachusetts 01605; and § University of Utah, Department of Bioengineering, Salt Lake City, Utah 84112
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