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Department of Biology, Yale University, New Haven, Connecticut 06520-8103
Mutants in the meiosis-specific RED1 gene
of S. cerevisiae fail to make any synaptonemal complex
(SC) or any obvious precursors to the SC. Using antibodies that specifically recognize the Red1 protein,
Red1 has been localized along meiotic pachytene chromosomes. Red1 also localizes to the unsynapsed axial
elements present in a zip1 mutant, suggesting that Red1
is a component of the lateral elements of mature SCs.
Anti-Red1 staining is confined to the cores of meiotic
chromosomes and is not associated with the loops of
chromatin that lie outside the SC. Analysis of the spo11
mutant demonstrates that Red1 localization does not
depend upon meiotic recombination. The localization
of Red1 has been compared with two other meiosisspecific components of chromosomes, Hop1 and Zip1;
Zip1 serves as a marker for synapsed chromosomes.
Double labeling of wild-type meiotic chromosomes
with anti-Zip1 and anti-Red1 antibodies demonstrates
that Red1 localizes to chromosomes both before and
during pachytene. Double labeling with anti-Hop1 and
anti-Red1 antibodies reveals that Hop1 protein localizes only in areas that also contain Red1, and studies of
Hop1 localization in a red1 null mutant demonstrate
that Hop1 localization depends on Red1 function.
These observations are consistent with previous genetic
studies suggesting that Red1 and Hop1 directly interact. There is little or no Hop1 protein on pachytene
chromosomes or in synapsed chromosomal regions.
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