Genetic Analysis of Myoblast Fusion: blown fuse Is Required for Progression Beyond the Prefusion Complex

doi:10.1083/jcb.136.6.1249

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© The Rockefeller University Press, 0021-9525/1997//1249 $5.00
The Journal of Cell Biology, Volume 136, Number 6, , 1997 1249-1261

Article

Genetic Analysis of Myoblast Fusion: blown fuse Is Required for Progression Beyond the Prefusion Complex

Stephen K. Doberstein, Richard D. Fetter, Anand Y. Mehta, and Corey S. Goodman

Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720

The events of myoblast fusion in Drosophila are dissected hereby combining genetic analysis with light and electron microscopy.We describe a new and essential intermediate step in the process,the formation of a prefusion complex consisting of "paired vesicles."These pairs of vesicles from different cells align with eachother across apposed plasma membranes. This prefusion complexresolves into dense membrane plaques between apposed cells;these cells then establish cytoplasmic continuity by fusionof small areas of plasma membrane followed by vesiculationof apposed membranes. Different steps in this process are specificallyblocked by mutations in four genes required for myoblast fusion.One of these genes, blown fuse, encodes a novel cytoplasmicprotein expressed in unfused myoblasts that is essential forprogression beyond the prefusion complex stage.

Abbreviations used in this paper: blow, blown fuse; mbc, myoblast city; PLP, periodate-lysine-paraformaldehyde; rost, rollingstone; RT, room temperature.

Please address all correspondence to C. Goodman, Howard HughesMedical Institute, Department of Molecular and Cell Biology,University of California, Berkeley, CA 94720.

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