© The Rockefeller University Press,
0021-9525/1997//1385 $5.00
The Journal of Cell Biology, Volume 136, Number 6,
, 1997 1385-1395
Integrin-mediated Activation of MAP Kinase Is Independent of FAK: Evidence for Dual Integrin Signaling Pathways in Fibroblasts
Tsung H. Lin*,
Andrew E. Aplin*,
Yu Shen
,
Qiming Chen*,
Michael Schaller
,
Lewis Romer
,||,
Ikramuddin Aukhil**, and
R.L. Juliano*
* Department of Pharmacology,
Department of Cell Biology,
Department of Pediatrics, || Department of Anesthesiology, School of Medicine, and ** Department of Periodontics, School of Dentistry, University of North Carolina, Chapel Hill, North Carolina 27599
Integrin-mediated cell adhesion causes activation of MAP kinases and increased tyrosine phosphorylation of focal adhesion kinase (FAK). Autophosphorylation of FAK leads to the binding of SH2-domain proteins including Src-family kinases and the Grb2–Sos complex. Since Grb2–Sos is a key regulator of the Ras signal transduction pathway, one plausible hypothesis has been that integrin-mediated tyrosine phosphorylation of FAK leads to activation of the Ras cascade and ultimately to mitogen activated protein (MAP) kinase activation. Thus, in this scenario FAK would serve as an upstream regulator of MAP kinase activity. However, in this report we present several lines of evidence showing that integrin-mediated MAP kinase activity in fibroblasts is independent of FAK. First, a β1 integrin subunit deletion mutant affecting the putative FAK binding site supports activation of MAP kinase in adhering fibroblasts but not tyrosine phosphorylation of FAK. Second, fibroblast adhesion to bacterially expressed fragments of fibronectin demonstrates that robust activation of MAP kinase can precede tyrosine phosphorylation of FAK. Finally, we have used FRNK, the noncatalytic COOH-terminal domain of FAK, as a dominant negative inhibitor of FAK autophosphorylation and of tyrosine phosphorylation of focal contacts. Using retroviral infection, we demonstrate that levels of FRNK expression sufficient to completely block FAK tyrosine phosphorylation were without effect on integrin-mediated activation of MAP kinase. These results strongly suggest that integrin-mediated activation of MAP kinase is independent of FAK and indicate the probable existence of at least two distinct integrin signaling pathways in fibroblasts.
Abbreviations used in this paper: FAK, pp125 focal adhesion kinase; Fn, fibronectin; FRNK, FAK-related nonkinase; MAP, mitogen activated protein; MEK, MAP kinase kinase; WT, wild type.
The EEEEYMPME epitope–tagged rat MEK-1 construct (EE-MEK) was kindly provided by Dennis J. Templeton (Case Western Reserve University, Cleveland, OH). A.R. Horwitz (University of Illinois, Urbana, IL) generously provided the chicken β1 constructs as well as the W1B10 monoclonal antibody.
Please address all correspondence to R.L. Juliano, Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599. Ph.: (919) 966-4383; Fax: (919) 966-5640.

CiteULike
Complore
Connotea
Del.icio.us
Digg
Facebook
Reddit
Technorati
Twitter What's this?
This article has been cited by other articles:
-
Yang, Y., Wu, X., Gui, P., Wu, J., Sheng, J.-Z., Ling, S., Braun, A. P., Davis, G. E., Davis, M. J.
(2010). {alpha}5{beta}1 Integrin Engagement Increases Large Conductance, Ca2+-activated K+ Channel Current and Ca2+ Sensitivity through c-src-mediated Channel Phosphorylation. J. Biol. Chem.
285: 131-141
[Abstract]
[Full Text]
-
Chaturvedi, L. S., Marsh, H. M., Basson, M. D.
(2007). Src and focal adhesion kinase mediate mechanical strain-induced proliferation and ERK1/2 phosphorylation in human H441 pulmonary epithelial cells. Am. J. Physiol. Cell Physiol.
292: C1701-C1713
[Abstract]
[Full Text]
-
Fielitz, J., Philipp, S., Herda, L. R., Schuch, E., Pilz, B., Schubert, C., Gunzler, V., Willenbrock, R., Regitz-Zagrosek, V.
(2007). Inhibition of prolyl 4-hydroxylase prevents left ventricular remodelling in rats with thoracic aortic banding. Eur J Heart Fail
9: 336-342
[Abstract]
[Full Text]
-
Sawhney, R. S., Cookson, M. M., Omar, Y., Hauser, J., Brattain, M. G.
(2006). Integrin {alpha}2-mediated ERK and Calpain Activation Play a Critical Role in Cell Adhesion and Motility via Focal Adhesion Kinase Signaling: IDENTIFICATION OF A NOVEL SIGNALING PATHWAY. J. Biol. Chem.
281: 8497-8510
[Abstract]
[Full Text]
-
Kanegae, K., Tamura, M., Kabashima, N., Serino, R., Tokunaga, M., Oikawa, S., Nakashima, Y.
(2005). Synergistic induction of monocyte chemoattractant protein-1 by integrins and platelet-derived growth factor via focal adhesion kinase in mesangial cells. Nephrol Dial Transplant
20: 2080-2088
[Abstract]
[Full Text]
-
Sheets, S. M., Potempa, J., Travis, J., Casiano, C. A., Fletcher, H. M.
(2005). Gingipains from Porphyromonas gingivalis W83 Induce Cell Adhesion Molecule Cleavage and Apoptosis in Endothelial Cells. Infect. Immun.
73: 1543-1552
[Abstract]
[Full Text]
-
Ding, Q., Grammer, J. R., Nelson, M. A., Guan, J.-L., Stewart, J. E. Jr., Gladson, C. L.
(2005). p27Kip1 and Cyclin D1 Are Necessary for Focal Adhesion Kinase Regulation of Cell Cycle Progression in Glioblastoma Cells Propagated in Vitro and in Vivo in the Scid Mouse Brain. J. Biol. Chem.
280: 6802-6815
[Abstract]
[Full Text]
-
Bill, H. M., Knudsen, B., Moores, S. L., Muthuswamy, S. K., Rao, V. R., Brugge, J. S., Miranti, C. K.
(2004). Epidermal Growth Factor Receptor-Dependent Regulation of Integrin-Mediated Signaling and Cell Cycle Entry in Epithelial Cells. Mol. Cell. Biol.
24: 8586-8599
[Abstract]
[Full Text]
-
Yang, J., Price, M. A., Neudauer, C. L., Wilson, C., Ferrone, S., Xia, H., Iida, J., Simpson, M. A., McCarthy, J. B.
(2004). Melanoma chondroitin sulfate proteoglycan enhances FAK and ERK activation by distinct mechanisms. JCB
165: 881-891
[Abstract]
[Full Text]
-
Sanders, M. A., Basson, M. D.
(2004). Collagen IV regulates Caco-2 migration and ERK activation via {alpha}1{beta}1- and {alpha}2{beta}1-integrin-dependent Src kinase activation. Am. J. Physiol. Gastrointest. Liver Physiol.
286: G547-G557
[Abstract]
[Full Text]
-
Chung, C.-H., Wu, W.-B., Huang, T.-F.
(2004). Aggretin, a snake venom-derived endothelial integrin {alpha}2{beta}1 agonist, induces angiogenesis via expression of vascular endothelial growth factor. Blood
103: 2105-2113
[Abstract]
[Full Text]
-
Stawowy, P., Kallisch, H., Veinot, J. P., Kilimnik, A., Prichett, W., Goetze, S., Seidah, N. G., Chretien, M., Fleck, E., Graf, K.
(2004). Endoproteolytic Activation of {alpha}v Integrin by Proprotein Convertase PC5 Is Required for Vascular Smooth Muscle Cell Adhesion to Vitronectin and Integrin-Dependent Signaling. Circulation
109: 770-776
[Abstract]
[Full Text]
-
Shima, T., Nada, S., Okada, M.
(2003). Transmembrane phosphoprotein Cbp senses cell adhesion signaling mediated by Src family kinase in lipid rafts. Proc. Natl. Acad. Sci. USA
100: 14897-14902
[Abstract]
[Full Text]
-
Moissoglu, K., Gelman, I. H.
(2003). v-Src Rescues Actin-based Cytoskeletal Architecture and Cell Motility and Induces Enhanced Anchorage Independence during Oncogenic Transformation of Focal Adhesion Kinase-null Fibroblasts. J. Biol. Chem.
278: 47946-47959
[Abstract]
[Full Text]
-
Hazlehurst, L. A., Enkemann, S. A., Beam, C. A., Argilagos, R. F., Painter, J., Shain, K. H., Saporta, S., Boulware, D., Moscinski, L., Alsina, M., Dalton, W. S.
(2003). Genotypic and Phenotypic Comparisons of de Novo and Acquired Melphalan Resistance in an Isogenic Multiple Myeloma Cell Line Model. Cancer Res.
63: 7900-7906
[Abstract]
[Full Text]
-
Balasubramanian, S., Kuppuswamy, D.
(2003). RGD-containing Peptides Activate S6K1 through {beta}3 Integrin in Adult Cardiac Muscle Cells. J. Biol. Chem.
278: 42214-42224
[Abstract]
[Full Text]
-
Laakko, T., Juliano, R. L.
(2003). Adhesion Regulation of Stromal Cell-derived Factor-1 Activation of ERK in Lymphocytes by Phosphatases. J. Biol. Chem.
278: 31621-31628
[Abstract]
[Full Text]
-
Wilson, S. H., Ljubimov, A. V., Morla, A. O., Caballero, S., Shaw, L. C., Spoerri, P. E., Tarnuzzer, R. W., Grant, M. B.
(2003). Fibronectin Fragments Promote Human Retinal Endothelial Cell Adhesion and Proliferation and ERK Activation through {alpha}5{beta}1 Integrin and PI 3-Kinase. IOVS
44: 1704-1715
[Abstract]
[Full Text]
-
Puente, L. G., Ostergaard, H. L.
(2003). {beta}1/{beta}3 integrin ligation is uncoupled from ERK1/ERK2 activation in cytotoxic T lymphocytes. J. Leukoc. Biol.
73: 391-398
[Abstract]
[Full Text]
-
Naranatt, P. P., Akula, S. M., Zien, C. A., Krishnan, H. H., Chandran, B.
(2003). Kaposi's Sarcoma-Associated Herpesvirus Induces the Phosphatidylinositol 3-Kinase-PKC-{zeta}-MEK-ERK Signaling Pathway in Target Cells Early during Infection: Implications for Infectivity. J. Virol.
77: 1524-1539
[Abstract]
[Full Text]
-
Liegibel, U. M., Sommer, U., Tomakidi, P., Hilscher, U., van den Heuvel, L., Pirzer, R., Hillmeier, J., Nawroth, P., Kasperk, C.
(2002). Concerted Action of Androgens and Mechanical Strain Shifts Bone Metabolism from High Turnover into an Osteoanabolic Mode. JEM
196: 1387-1392
[Abstract]
[Full Text]
-
Smedberg, J. L., Smith, E. R., Capo-chichi, C. D., Frolov, A., Yang, D.-H., Godwin, A. K., Xu, X.-X.
(2002). Ras/MAPK Pathway Confers Basement Membrane Dependence upon Endoderm Differentiation of Embryonic Carcinoma Cells. J. Biol. Chem.
277: 40911-40918
[Abstract]
[Full Text]
-
Sachs, K., Gifford, D., Jaakkola, T., Sorger, P., Lauffenburger, D. A.
(2002). Bayesian Network Approach to Cell Signaling Pathway Modeling. Sci Signal
2002: pe38-pe38
[Abstract]
[Full Text]
-
Monick, M. M., Powers, L., Butler, N., Yarovinsky, T., Hunninghake, G. W.
(2002). Interaction of matrix with integrin receptors is required for optimal LPS-induced MAP kinase activation. Am. J. Physiol. Lung Cell. Mol. Physiol.
283: L390-L402
[Abstract]
[Full Text]
-
Zhang, L., Bewick, M., Lafrenie, R. M.
(2002). Role of Raf-1 and FAK in cell density-dependent regulation of integrin-dependent activation of MAP kinase. Carcinogenesis
23: 1251-1258
[Abstract]
[Full Text]
-
Gu, J., Fujibayashi, A., Yamada, K. M., Sekiguchi, K.
(2002). Laminin-10/11 and Fibronectin Differentially Prevent Apoptosis Induced by Serum Removal via Phosphatidylinositol 3-Kinase/Akt- and MEK1/ERK-dependent Pathways. J. Biol. Chem.
277: 19922-19928
[Abstract]
[Full Text]
-
Aplin, A. E., Hogan, B. P., Tomeu, J., Juliano, R. L.
(2002). Cell adhesion differentially regulates the nucleocytoplasmic distribution of active MAP kinases. J. Cell Sci.
115: 2781-2790
[Abstract]
[Full Text]
-
Nony, P. A., Schnellmann, R. G.
(2001). Interactions between Collagen IV and Collagen-Binding Integrins in Renal Cell Repair after Sublethal Injury. Mol. Pharmacol.
60: 1226-1234
[Abstract]
[Full Text]
-
Hazlehurst, L. A., Valkov, N., Wisner, L., Storey, J. A., Boulware, D., Sullivan, D. M., Dalton, W. S.
(2001). Reduction in drug-induced DNA double-strand breaks associated with {beta}1 integrin-mediated adhesion correlates with drug resistance in U937 cells. Blood
98: 1897-1903
[Abstract]
[Full Text]
-
Gleeson, L. M., Chakraborty, C., McKinnon, T., Lala, P. K.
(2001). Insulin-Like Growth Factor-Binding Protein 1 Stimulates Human Trophoblast Migration by Signaling through {{alpha}}5{beta}1 Integrin via Mitogen-Activated Protein Kinase Pathway. J. Clin. Endocrinol. Metab.
86: 2484-2493
[Abstract]
[Full Text]
-
Keller, R. S., Shai, S.-Y., Babbitt, C. J., Pham, C. G., Solaro, R. J., Valencik, M. L., Loftus, J. C., Ross, R. S.
(2001). Disruption of Integrin Function in the Murine Myocardium Leads to Perinatal Lethality, Fibrosis, and Abnormal Cardiac Performance. Am. J. Pathol.
158: 1079-1090
[Abstract]
[Full Text]
-
Li, W., Duzgun, A., Sumpio, B. E., Basson, M. D.
(2001). Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells. Am. J. Physiol. Gastrointest. Liver Physiol.
280: G75-G87
[Abstract]
[Full Text]
-
Scholle, F., Bendt, K. M., Raab-Traub, N.
(2000). Epstein-Barr Virus LMP2A Transforms Epithelial Cells, Inhibits Cell Differentiation, and Activates Akt. J. Virol.
74: 10681-10689
[Abstract]
[Full Text]
-
Willeke, T., Behrens, S., Scharffetter-Kochanek, K., Gaehtgens, P., Walzog, B.
(2000). {beta}2 Integrin (CD11/CD18)-mediated signaling involves tyrosine phosphorylation of c-Cbl in human neutrophils. J. Leukoc. Biol.
68: 284-292
[Abstract]
[Full Text]
-
Gatzka, M., Prisco, M., Baserga, R.
(2000). Stabilization of the Ras Oncoprotein by the Insulin-like Growth Factor 1 Receptor during Anchorage-independent Growth. Cancer Res.
60: 4222-4230
[Abstract]
[Full Text]
-
Fornaro, M., Steger, C. A., Bennett, A. M., Wu, J. J., Languino, L. R.
(2000). Differential Role of beta 1C and beta 1A Integrin Cytoplasmic Variants in Modulating Focal Adhesion Kinase, Protein Kinase B/AKT, and Ras/Mitogen-activated Protein Kinase Pathways. Mol. Biol. Cell
11: 2235-2249
[Abstract]
[Full Text]
-
Yu, C. F., Sanders, M. A., Basson, M. D.
(2000). Human Caco-2 motility redistributes FAK and paxillin and activates p38 MAPK in a matrix-dependent manner. Am. J. Physiol. Gastrointest. Liver Physiol.
278: G952-G966
[Abstract]
[Full Text]
-
Schmid, R.-S., Pruitt, W. M., Maness, P. F.
(2000). A MAP Kinase-Signaling Pathway Mediates Neurite Outgrowth on L1 and Requires Src-Dependent Endocytosis. J. Neurosci.
20: 4177-4188
[Abstract]
[Full Text]
-
Buensuceso, C. S., O'Toole, T. E.
(2000). The Association of CRKII with C3G Can be Regulated by Integrins and Defines a Novel Means to Regulate the Mitogen-activated Protein Kinases. J. Biol. Chem.
275: 13118-13125
[Abstract]
[Full Text]
-
Martin-Bermudo, M.
(2000). Integrins modulate the Egfr signaling pathway to regulate tendon cell differentiation in the Drosophila embryo. Development
127: 2607-2615
[Abstract]
-
Tamura, M., Gu, J., Tran, H., Yamada, K. M.
(1999). PTEN Gene and Integrin Signaling in Cancer. JNCI J Natl Cancer Inst
91: 1820-1828
[Abstract]
[Full Text]
-
Renshaw, M. W., Price, L. S., Schwartz, M. A.
(1999). Focal Adhesion Kinase Mediates the Integrin Signaling Requirement for Growth Factor Activation of Map Kinase. JCB
147: 611-618
[Abstract]
[Full Text]
-
Aplin, A. E., Short, S. M., Juliano, R. L.
(1999). Anchorage-dependent Regulation of the Mitogen-activated Protein Kinase Cascade by Growth Factors Is Supported by a Variety of Integrin alpha Chains. J. Biol. Chem.
274: 31223-31228
[Abstract]
[Full Text]
-
Kapila, Y. L., Wang, S., Johnson, P. W.
(1999). Mutations in the Heparin Binding Domain of Fibronectin in Cooperation with the V Region Induce Decreases in pp125FAK Levels Plus Proteoglycan-mediated Apoptosis via Caspases. J. Biol. Chem.
274: 30906-30913
[Abstract]
[Full Text]
-
Esparza, J., Vilardell, C., Calvo, J., Juan, M., Vives, J., Urbano-Marquez, A., Yague, J., Cid, M. C.
(1999). Fibronectin Upregulates Gelatinase B (MMP-9) and Induces Coordinated Expression of Gelatinase A (MMP-2) and Its Activator MT1-MMP (MMP-14) by Human T Lymphocyte Cell Lines. A Process Repressed Through RAS/MAP Kinase Signaling Pathways. Blood
94: 2754-2766
[Abstract]
[Full Text]
-
Ghiso, J. A. A., Kovalski, K., Ossowski, L.
(1999). Tumor Dormancy Induced by Downregulation of Urokinase Receptor in Human Carcinoma Involves Integrin and MAPK Signaling. JCB
147: 89-104
[Abstract]
[Full Text]
-
Asthagiri, A. R., Nelson, C. M., Horwitz, A. F., Lauffenburger, D. A.
(1999). Quantitative Relationship among Integrin-Ligand Binding, Adhesion, and Signaling via Focal Adhesion Kinase and Extracellular Signal-regulated Kinase 2. J. Biol. Chem.
274: 27119-27127
[Abstract]
[Full Text]
-
Giancotti, F. G., Ruoslahti, E.
(1999). Integrin Signaling. Science
285: 1028-1033
[Abstract]
[Full Text]
-
Gu, J., Tamura, M., Pankov, R., Danen, E. H.J., Takino, T., Matsumoto, K., Yamada, K. M.
(1999). Shc and Fak Differentially Regulate Cell Motility and Directionality Modulated by Pten. JCB
146: 389-404
[Abstract]
[Full Text]
-
Tamura, M., Gu, J., Danen, E. H. J., Takino, T., Miyamoto, S., Yamada, K. M.
(1999). PTEN Interactions with Focal Adhesion Kinase and Suppression of the Extracellular Matrix-dependent Phosphatidylinositol 3-Kinase/Akt Cell Survival Pathway. J. Biol. Chem.
274: 20693-20703
[Abstract]
[Full Text]
-
Zhu, A. J., Haase, I., Watt, F. M.
(1999). Signaling via beta 1 integrins and mitogen-activated protein kinase determines human epidermal stem cell fate in vitro. Proc. Natl. Acad. Sci. USA
96: 6728-6733
[Abstract]
[Full Text]
-
van de Water, B., Nagelkerke, J. F., Stevens, J. L.
(1999). Dephosphorylation of Focal Adhesion Kinase (FAK) and Loss of Focal Contacts Precede Caspase-mediated Cleavage of FAK during Apoptosis in Renal Epithelial Cells. J. Biol. Chem.
274: 13328-13337
[Abstract]
[Full Text]
-
Miranti, C. K., Ohno, S., Brugge, J. S.
(1999). Protein Kinase C Regulates Integrin-induced Activation of the Extracellular Regulated Kinase Pathway Upstream of Shc. J. Biol. Chem.
274: 10571-10581
[Abstract]
[Full Text]
-
Farrelly, N., Lee, Y.-J., Oliver, J., Dive, C., Streuli, C. H.
(1999). Extracellular Matrix Regulates Apoptosis in Mammary Epithelium through a Control on Insulin Signaling. JCB
144: 1337-1348
[Abstract]
[Full Text]
-
Sastry, S. K., Lakonishok, M., Wu, S., Truong, T. Q., Huttenlocher, A., Turner, C. E., Horwitz, A. F.
(1999). Quantitative Changes in Integrin and Focal Adhesion Signaling Regulate Myoblast Cell Cycle Withdrawal. JCB
144: 1295-1309
[Abstract]
[Full Text]
-
Martin-Bermudo, M. D., Brown, N. H.
(1999). Uncoupling integrin adhesion and signaling: the beta PS cytoplasmic domain is sufficient to regulate gene expression in the Drosophila embryo. Genes Dev.
13: 729-739
[Abstract]
[Full Text]
-
Manabe, R.-i., Oh-e, N., Sekiguchi, K.
(1999). Alternatively Spliced EDA Segment Regulates Fibronectin-dependent Cell Cycle Progression and Mitogenic Signal Transduction. J. Biol. Chem.
274: 5919-5924
[Abstract]
[Full Text]
-
Garton, A. J., Tonks, N. K.
(1999). Regulation of Fibroblast Motility by the Protein Tyrosine Phosphatase PTP-PEST. J. Biol. Chem.
274: 3811-3818
[Abstract]
[Full Text]
-
Aplin, A., Juliano, R.
(1999). Integrin and cytoskeletal regulation of growth factor signaling to the MAP kinase pathway. J. Cell Sci.
112: 695-706
[Abstract]
-
Hackeng, C. M., Pladet, M. W., Akkerman, J.-W. N., van Rijn, H. J. M.
(1999). Low Density Lipoprotein Phosphorylates the Focal Adhesion-associated Kinase p125FAK in Human Platelets Independent of Integrin alpha IIbbeta 3. J. Biol. Chem.
274: 384-388
[Abstract]
[Full Text]
-
Franceschi, R.T.
(1999). The Developmental Control of Osteoblast-Specific Gene Expression: Role of Specific Transcription Factors and the Extracellular Matrix Environment. CROBM
10: 40-57
[Abstract]
[Full Text]
-
Tamura, M., Gu, J., Takino, T., Yamada, K. M.
(1999). Tumor Suppressor PTEN Inhibition of Cell Invasion, Migration, and Growth: Differential Involvement of Focal Adhesion Kinase and p130Cas. Cancer Res.
59: 442-449
[Abstract]
[Full Text]
-
Zhao, J.-H., Reiske, H., Guan, J.-L.
(1998). Regulation of the Cell Cycle by Focal Adhesion Kinase. JCB
143: 1997-2008
[Abstract]
[Full Text]
-
Xiao, G., Wang, D., Benson, M. D., Karsenty, G., Franceschi, R. T.
(1998). Role of the alpha 2-Integrin in Osteoblast-specific Gene Expression and Activation of the Osf2 Transcription Factor. J. Biol. Chem.
273: 32988-32994
[Abstract]
[Full Text]
-
Gu, J., Tamura, M., Yamada, K. M.
(1998). Tumor Suppressor PTEN Inhibits Integrin- and Growth Factor-mediated Mitogen-activated Protein (MAP) Kinase Signaling Pathways. JCB
143: 1375-1383
[Abstract]
[Full Text]
-
Lebrun, P., Mothe-Satney, I., Delahaye, L., Van Obberghen, E., Baron, V.
(1998). Insulin Receptor Substrate-1 as a Signaling Molecule for Focal Adhesion Kinase pp125FAK and pp60src. J. Biol. Chem.
273: 32244-32253
[Abstract]
[Full Text]
-
Howe, A. K., Juliano, R. L.
(1998). Distinct Mechanisms Mediate the Initial and Sustained Phases of Integrin-mediated Activation of the Raf/MEK/Mitogen-activated Protein Kinase Cascade. J. Biol. Chem.
273: 27268-27274
[Abstract]
[Full Text]
-
Chen, H.-C., Chan, P.-C., Tang, M.-J., Cheng, C.-H., Chang, T.-J.
(1998). Tyrosine Phosphorylation of Focal Adhesion Kinase Stimulated by Hepatocyte Growth Factor Leads to Mitogen-activated Protein Kinase Activation. J. Biol. Chem.
273: 25777-25782
[Abstract]
[Full Text]
-
Miralem, T., Templeton, D. M.
(1998). Inactivation of kinase cascades in mesangial cells grown on collagen type I. Am. J. Physiol. Renal Physiol.
275: F585-F594
[Abstract]
[Full Text]
-
Short, S. M., Talbott, G. A., Juliano, R. L.
(1998). Integrin-mediated Signaling Events in Human Endothelial Cells. Mol. Biol. Cell
9: 1969-1980
[Abstract]
[Full Text]
-
Law, S. F., Zhang, Y.-Z., Klein-Szanto, A. J. P., Golemis, E. A.
(1998). Cell Cycle-Regulated Processing of HEF1 to Multiple Protein Forms Differentially Targeted to Multiple Subcellular Compartments. Mol. Cell. Biol.
18: 3540-3551
[Abstract]
[Full Text]
-
Aplin, A. E., Howe, A., Alahari, S. K., Juliano, R. L.
(1998). Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins. Pharmacol. Rev.
50: 197-264
[Abstract]
[Full Text]
-
Tsuda, M., Matozaki, T., Fukunaga, K., Fujioka, Y., Imamoto, A., Noguchi, T., Takada, T., Yamao, T., Takeda, H., Ochi, F., Yamamoto, T., Kasuga, M.
(1998). Integrin-mediated Tyrosine Phosphorylation of SHPS-1 and Its Association with SHP-2. ROLES OF Fak AND Src FAMILY KINASES. J. Biol. Chem.
273: 13223-13229
[Abstract]
[Full Text]
-
Kheradmand, F., Werner, E., Tremble, P., Symons, M., Werb, Z.
(1998). Role of Rac1 and Oxygen Radicals in Collagenase-1 Expression Induced by Cell Shape Change. Science
280: 898-902
[Abstract]
[Full Text]
-
Schlaepfer, D. D., Jones, K. C., Hunter, T.
(1998). Multiple Grb2-Mediated Integrin-Stimulated Signaling Pathways to ERK2/Mitogen-Activated Protein Kinase: Summation of Both c-Src- and Focal Adhesion Kinase-Initiated Tyrosine Phosphorylation Events. Mol. Cell. Biol.
18: 2571-2585
[Abstract]
[Full Text]
-
Staubli, U., Chun, D., Lynch, G.
(1998). Time-Dependent Reversal of Long-Term Potentiation by an Integrin Antagonist. J. Neurosci.
18: 3460-3469
[Abstract]
[Full Text]
-
Wei, J., Shaw, L. M., Mercurio, A. M.
(1998). Regulation of Mitogen-activated Protein Kinase Activation by the Cytoplasmic Domain of the alpha 6 Integrin Subunit. J. Biol. Chem.
273: 5903-5907
[Abstract]
[Full Text]
-
Cary, L. A., Han, D. C., Polte, T. R., Hanks, S. K., Guan, J.-L.
(1998). Identification of p130Cas as a Mediator of Focal Adhesion Kinase-promoted Cell Migration. JCB
140: 211-221
[Abstract]
[Full Text]
-
Wennerberg, K, Fassler, R, Warmegard, B, Johansson, S
(1998). Mutational analysis of the potential phosphorylation sites in the cytoplasmic domain of integrin beta1A. Requirement for threonines 788-789 in receptor activation. J. Cell Sci.
111: 1117-1126
[Abstract]
-
Schaller, M. D., Sasaki, T.
(1997). Differential Signaling by the Focal Adhesion Kinase and Cell Adhesion Kinase beta. J. Biol. Chem.
272: 25319-25325
[Abstract]
[Full Text]
-
Barberis, L., Wary, K. K., Fiucci, G., Liu, F., Hirsch, E., Brancaccio, M., Altruda, F., Tarone, G., Giancotti, F. G.
(2000). Distinct Roles of the Adaptor Protein Shc and Focal Adhesion Kinase in Integrin Signaling to ERK. J. Biol. Chem.
275: 36532-36540
[Abstract]
[Full Text]
-
Sanders, M. A., Basson, M. D.
(2000). Collagen IV-dependent ERK Activation in Human Caco-2 Intestinal Epithelial Cells Requires Focal Adhesion Kinase. J. Biol. Chem.
275: 38040-38047
[Abstract]
[Full Text]
-
Laser, M., Willey, C. D., Jiang, W., Cooper, G. IV, Menick, D. R., Zile, M. R., Kuppuswamy, D.
(2000). Integrin Activation and Focal Complex Formation in Cardiac Hypertrophy. J. Biol. Chem.
275: 35624-35630
[Abstract]
[Full Text]
-
Matter, M. L., Ruoslahti, E.
(2001). A Signaling Pathway from the alpha 5beta 1 and alpha vbeta 3 Integrins That Elevates bcl-2 Transcription. J. Biol. Chem.
276: 27757-27763
[Abstract]
[Full Text]
-
Nguyen, D. H. D., Webb, D. J., Catling, A. D., Song, Q., Dhakephalkar, A., Weber, M. J., Ravichandran, K. S., Gonias, S. L.
(2000). Urokinase-type Plasminogen Activator Stimulates the Ras/Extracellular Signal-regulated Kinase (ERK) Signaling Pathway and MCF-7 Cell Migration by a Mechanism That Requires Focal Adhesion Kinase, Src, and Shc. RAPID DISSOCIATION OF GRB2/SOS-SHC COMPLEX IS ASSOCIATED WITH THE TRANSIENT PHOSPHORYLATION OF ERK IN UROKINASE-TREATED CELLS. J. Biol. Chem.
275: 19382-19388
[Abstract]
[Full Text]