J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/04/169/14 $2.00
Volume 137, Number 1, April 7, 1997 169-182

cdc12p, a Protein Required for Cytokinesis in Fission Yeast, Is a Component of the Cell Division Ring and Interacts with Profilin

Fred Chang,^* David Drubin, and Paul Nurse^*

^* Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom; and  Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202

As in many other eukaryotic cells, cell division in fission yeast depends on the assembly of an actin ring that circumscribes the middle of the cell. Schizosaccharomyces pombe cdc12 is an essential gene necessary for actin ring assembly and septum formation. Here we show that cdc12p is a member of a family of proteins including Drosophila diaphanous, Saccharomyces cerevisiae BNI1, and S. pombe fus1, which are involved in cytokinesis or other actin-mediated processes. Using indirect immunofluorescence, we show that cdc12p is located in the cell division ring and not in other actin structures. When overexpressed, cdc12p is located at a medial spot in interphase that anticipates the future ring site. cdc12p localization is altered in actin ring mutants. cdc8 (tropomyosin homologue), cdc3 (profilin homologue), and cdc15 mutants exhibit no specific cdc12p staining during mitosis. cdc4 mutant cells exhibit a medial cortical cdc12p spot in place of a ring. mid1 mutant cells generally exhibit a cdc12p spot with a single cdc12p strand extending in a random direction. Based on these patterns, we present a model in which ring assembly originates from a single point on the cortex and in which a molecular pathway for the functions of cytokinesis proteins is suggested. Finally, we found that cdc12 and cdc3 mutants show a syntheticlethal genetic interaction, and a proline-rich domain of cdc12p binds directly to profilin cdc3p in vitro, suggesting that one function of cdc12p in ring assembly is to bind profilin.

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• Buttery, S. M., Yoshida, S., Pellman, D. (2007). Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables. Mol. Biol. Cell 18: 1826-1838 [Abstract] [Full Text]  
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• Moseley, J. B., Goode, B. L. (2006). The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism. Microbiol. Mol. Biol. Rev. 70: 605-645 [Abstract] [Full Text]  
• Wu, J.-Q., Sirotkin, V., Kovar, D. R., Lord, M., Beltzner, C. C., Kuhn, J. R., Pollard, T. D. (2006). Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. JCB 174: 391-402 [Abstract] [Full Text]  
• Wachtler, V., Huang, Y., Karagiannis, J., Balasubramanian, M. K. (2006). Cell Cycle-dependent Roles for the FCH-Domain Protein Cdc15p in Formation of the Actomyosin Ring in Schizosaccharomyces pombe. Mol. Biol. Cell 17: 3254-3266 [Abstract] [Full Text]  
• Bugyi, B., Papp, G., Hild, G., Lorinczy, D., Nevalainen, E. M., Lappalainen, P., Somogyi, B., Nyitrai, M. (2006). Formins Regulate Actin Filament Flexibility through Long Range Allosteric Interactions. J. Biol. Chem. 281: 10727-10736 [Abstract] [Full Text]  
• Nakano, K., Mabuchi, I. (2006). Actin-depolymerizing Protein Adf1 Is Required for Formation and Maintenance of the Contractile Ring during Cytokinesis in Fission Yeast. Mol. Biol. Cell 17: 1933-1945 [Abstract] [Full Text]  
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• Takeya, R., Sumimoto, H. (2003). Fhos, a mammalian formin, directly binds to F-actin via a region N-terminal to the FH1 domain and forms a homotypic complex via the FH2 domain to promote actin fiber formation. J. Cell Sci. 116: 4567-4575 [Abstract] [Full Text]  
• Carnahan, R. H., Gould, K. L. (2003). The PCH family protein, Cdc15p, recruits two F-actin nucleation pathways to coordinate cytokinetic actin ring formation in Schizosaccharomyces pombe. JCB 162: 851-862 [Abstract] [Full Text]  
• Bito, H. (2003). Dynamic Control of Neuronal Morphogenesis by Rho Signaling. J Biochem 134: 315-319 [Abstract] [Full Text]  
• Evangelista, M., Zigmond, S., Boone, C. (2003). Formins: signaling effectors for assembly and polarization of actin filaments. J. Cell Sci. 116: 2603-2611 [Abstract] [Full Text]  
• Kovar, D. R., Kuhn, J. R., Tichy, A. L., Pollard, T. D. (2003). The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin. JCB 161: 875-887 [Abstract] [Full Text]  
• Koka, S., Neudauer, C. L., Li, X., Lewis, R. E., McCarthy, J. B., Westendorf, J. J. (2003). The formin-homology-domain-containing protein FHOD1 enhances cell migration. J. Cell Sci. 116: 1745-1755 [Abstract] [Full Text]  
• Kitayama, C., Uyeda, T. Q. P. (2003). ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum. J. Cell Sci. 116: 711-723 [Abstract] [Full Text]  
• Copeland, J. W., Treisman, R. (2002). The Diaphanous-related Formin mDia1 Controls Serum Response Factor Activity through its Effects on Actin Polymerization. Mol. Biol. Cell 13: 4088-4099 [Abstract] [Full Text]  
• Karagiannis, J., Oulton, R., Young, P. G. (2002). The Scw1 RNA-Binding Domain Protein Regulates Septation and Cell-Wall Structure in Fission Yeast. Genetics 162: 45-58 [Abstract] [Full Text]  
• Chattopadhyay, M. K., Tabor, C. W., Tabor, H. (2002). Absolute requirement of spermidine for growth and cell cycle progression of fission yeast (Schizosaccharomycespombe). Proc. Natl. Acad. Sci. USA 99: 10330-10334 [Abstract] [Full Text]  
• Guertin, D. A., Trautmann, S., McCollum, D. (2002). Cytokinesis in Eukaryotes. Microbiol. Mol. Biol. Rev. 66: 155-178 [Abstract] [Full Text]  
• Krebs, A., Rothkegel, M., Klar, M., Jockusch, B. M. (2002). Characterization of functional domains of mDia1, a link between the small GTPase Rho and the actin cytoskeleton. J. Cell Sci. 114: 3663-3672 [Abstract] [Full Text]  
• Liu, J., Tang, X., Wang, H., Oliferenko, S., Balasubramanian, M. K. (2002). The Localization of the Integral Membrane Protein Cps1p to the Cell Division Site is Dependent on the Actomyosin Ring and the Septation-Inducing Network in Schizosaccharomyces pombe. Mol. Biol. Cell 13: 989-1000 [Abstract] [Full Text]  
• Miyagi, Y., Yamashita, T., Fukaya, M., Sonoda, T., Okuno, T., Yamada, K., Watanabe, M., Nagashima, Y., Aoki, I., Okuda, K., Mishina, M., Kawamoto, S. (2002). Delphilin: a Novel PDZ and Formin Homology Domain-Containing Protein that Synaptically Colocalizes and Interacts with Glutamate Receptor delta 2 Subunit. J. Neurosci. 22: 803-814 [Abstract] [Full Text]  
• Nakano, K., Imai, J., Arai, R., Toh-e, A., Matsui, Y., Mabuchi, I. (2002). The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeast. J. Cell Sci. 115: 4629-4639 [Abstract] [Full Text]  
• Arai, R., Mabuchi, I. (2002). F-actin ring formation and the role of F-actin cables in the fission yeast Schizosaccharomyces pombe. J. Cell Sci. 115: 887-898 [Abstract] [Full Text]  
• Nakano, K., Satoh, K., Morimatsu, A., Ohnuma, M., Mabuchi, I. (2001). Interactions among a Fimbrin, a Capping Protein, and an Actin-depolymerizing Factor in Organization of the Fission Yeast Actin Cytoskeleton. Mol. Biol. Cell 12: 3515-3526 [Abstract] [Full Text]  
• Wu, J.-Q., Bähler, J., Pringle, J. R. (2001). Roles of a Fimbrin and an {alpha}-Actinin-like Protein in Fission Yeast Cell Polarization and Cytokinesis. Mol. Biol. Cell 12: 1061-1077 [Abstract] [Full Text]  
• Lu, J., Pollard, T. D. (2001). Profilin Binding to Poly-L-Proline and Actin Monomers along with Ability to Catalyze Actin Nucleotide Exchange Is Required for Viability of Fission Yeast. Mol. Biol. Cell 12: 1161-1175 [Abstract] [Full Text]  
• Paoletti, A., Chang, F. (2000). Analysis of mid1p, a Protein Required for Placement of the Cell Division Site, Reveals a Link between the Nucleus and the Cell Surface in Fission Yeast. Mol. Biol. Cell 11: 2757-2773 [Abstract] [Full Text]  
• Jantsch-Plunger, V., Gonczy, P., Romano, A., Schnabel, H., Hamill, D., Schnabel, R., Hyman, A. A., Glotzer, M. (2000). CYK-4: A Rho Family GTPase Activating Protein (GAP) Required for Central Spindle Formation and Cytokinesis. JCB 149: 1391-1404 [Abstract] [Full Text]  
• Vallen, E. A., Caviston, J., Bi, E. (2000). Roles of Hof1p, Bni1p, Bnr1p, and Myo1p in Cytokinesis in Saccharomyces cerevisiae. Mol. Biol. Cell 11: 593-611 [Abstract] [Full Text]  
• Le Goff, X, Motegi, F, Salimova, E, Mabuchi, I, Simanis, V (2000). The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p. J. Cell Sci. 113: 4157-4163 [Abstract]  
• Wong, K., Naqvi, N., Iino, Y, Yamamoto, M, Balasubramanian, M. (2000). Fission yeast Rng3p: an UCS-domain protein that mediates myosin II assembly during cytokinesis. J. Cell Sci. 113: 2421-2432 [Abstract]  
• Balasubramanian, M., McCollum, D, Surana, U (2000). Tying the knot: linking cytokinesis to the nuclear cycle. J. Cell Sci. 113: 1503-1513 [Abstract]  
• Motegi, F, Nakano, K, Mabuchi, I (2000). Molecular mechanism of myosin-II assembly at the division site in Schizosaccharomyces pombe. J. Cell Sci. 113: 1813-1825 [Abstract]  
• Afshar, K, Stuart, B, Wasserman, S. (2000). Functional analysis of the Drosophila diaphanous FH protein in early embryonic development. Development 127: 1887-1897 [Abstract]  
• Liu, J., Wang, H., McCollum, D., Balasubramanian, M. K. (1999). Drc1p/Cps1p, a 1,3-{beta}-Glucan Synthase Subunit, Is Essential for Division Septum Assembly in Schizosaccharomyces pombe. Genetics 153: 1193-1203 [Abstract] [Full Text]  
• Prokopenko, S. N., Brumby, A., O'Keefe, L., Prior, L., He, Y., Saint, R., Bellen, H. J. (1999). A putative exchange factor for Rho1 GTPase is required for initiation of cytokinesis in Drosophila. Genes Dev. 13: 2301-2314 [Abstract] [Full Text]  
• Jia, Z., Young, P. G. (1999). Ssp1 Promotes Actin Depolymerization and Is Involved in Stress Response and New End Take-Off Control in Fission Yeast. Mol. Biol. Cell 10: 1495-1510 [Abstract] [Full Text]  
• Furge, K. A., Cheng, Q.-c., Jwa, M., Shin, S., Song, K., Albright, C. F. (1999). Regions of Byr4, a Regulator of Septation in Fission Yeast, That Bind Spg1 or Cdc16 and Form a Two-component GTPase-activating Protein with Cdc16. J. Biol. Chem. 274: 11339-11343 [Abstract] [Full Text]  
• Lee, L., Klee, S. K., Evangelista, M., Boone, C., Pellman, D. (1999). Control of Mitotic Spindle Position by the Saccharomyces cerevisiae Formin Bni1p. JCB 144: 947-961 [Abstract] [Full Text]  
• Johnson, D. I. (1999). Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity. Microbiol. Mol. Biol. Rev. 63: 54-105 [Abstract] [Full Text]  
• Harris, S. D., Hofmann, A. F., Tedford, H. W., Lee, M. P. (1999). Identification and Characterization of Genes Required for Hyphal Morphogenesis in the Filamentous Fungus Aspergillus nidulans. Genetics 151: 1015-1025 [Abstract] [Full Text]  
• Wellington, A, Emmons, S, James, B, Calley, J, Grover, M, Tolias, P, Manseau, L (1999). Spire contains actin binding domains and is related to ascidian posterior end mark-5. Development 126: 5267-5274 [Abstract]  
• Kaiser, D., Vinson, V., Murphy, D., Pollard, T. (1999). Profilin is predominantly associated with monomeric actin in Acanthamoeba. J. Cell Sci. 112: 3779-3790 [Abstract]  
• Giansanti, M., Bonaccorsi, S, Gatti, M (1999). The role of anillin in meiotic cytokinesis of Drosophila males. J. Cell Sci. 112: 2323-2334 [Abstract]  
• Lippincott, J., Li, R. (1998). Dual Function of Cyk2, a cdc15/PSTPIP Family Protein, in Regulating Actomyosin Ring Dynamics and Septin Distribution. JCB 143: 1947-1960 [Abstract] [Full Text]  
• Bahler, J., Steever, A. B., Wheatley, S., Wang, Y.-l., Pringle, J. R., Gould, K. L., McCollum, D. (1998). Role of Polo Kinase and Mid1p in Determining the Site of Cell Division in Fission Yeast. JCB 143: 1603-1616 [Abstract] [Full Text]  
• Kamei, T., Tanaka, K., Hihara, T., Umikawa, M., Imamura, H., Kikyo, M., Ozaki, K., Takai, Y. (1998). Interaction of Bnr1p with a Novel Src Homology 3 Domain-containing Hof1p. IMPLICATION IN CYTOKINESIS IN SACCHAROMYCES CEREVISIAE. J. Biol. Chem. 273: 28341-28345 [Abstract] [Full Text]  
• Demeter, J., Sazer, S. (1998). imp2, a New Component of the Actin Ring in the Fission Yeast Schizosaccharomyces pombe. JCB 143: 415-427 [Abstract] [Full Text]  
• Bear, J. E., Rawls, J. F., Saxe, C. L. III (1998). SCAR, a WASP-related Protein, Isolated as a Suppressor of Receptor Defects in Late Dictyostelium Development. JCB 142: 1325-1335 [Abstract] [Full Text]  
• Balasubramanian, M. K., McCollum, D., Chang, L., Wong, K. C. Y., Naqvi, N. I., He, X., Sazer, S., Gould, K. L. (1998). Isolation and Characterization of New Fission Yeast Cytokinesis Mutants. Genetics 149: 1265-1275 [Abstract] [Full Text]  
• Swan, K., Severson, A., Carter, J., Martin, P., Schnabel, H, Schnabel, R, Bowerman, B (1998). cyk-1: a C. elegans FH gene required for a late step in embryonic cytokinesis. J. Cell Sci. 111: 2017-2027 [Abstract]  
• Petersen, J., Nielsen, O., Egel, R., Hagan, I. M. (1998). FH3, A Domain Found in Formins, Targets the Fission Yeast Formin Fus1 to the Projection Tip During Conjugation. JCB 141: 1217-1228 [Abstract] [Full Text]  
• Clarke, S. R., Staiger, C. J., Gibbon, B. C., Franklin-Tong, V. E. (1998). A Potential Signaling Role for Profilin in Pollen of Papaver rhoeas. Plant Cell 10: 967-980 [Abstract] [Full Text]  
• Fujiwara, T., Tanaka, K., Mino, A., Kikyo, M., Takahashi, K., Shimizu, K., Takai, Y. (1998). Rho1p-Bni1p-Spa2p Interactions: Implication in Localization of Bni1p at the Bud Site and Regulation of the Actin Cytoskeleton in Saccharomyces cerevisiae. Mol. Biol. Cell 9: 1221-1233 [Abstract] [Full Text]  
• Giansanti, M. G., Bonaccorsi, S., Williams, B., Williams, E. V., Santolamazza, C., Goldberg, M. L., Gatti, M. (1998). Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis. Genes Dev. 12: 396-410 [Abstract] [Full Text]  
• Petersen, J, Nielsen, O, Egel, R, Hagan, I. (1998). F-actin distribution and function during sexual differentiation in Schizosaccharomyces pombe. J. Cell Sci. 111: 867-876 [Abstract]  
• Gould, K. L., Simanis, V. (1997). The control of septum formation in fission yeast. Genes Dev. 11: 2939-2951 [Full Text]  
• Arellano, M, Duran, A, Perez, P (1997). Localisation of the Schizosaccharomyces pombe rho1p GTPase and its involvement in the organisation of the actin cytoskeleton. J. Cell Sci. 110: 2547-2555 [Abstract]  
• Alberts, A. S. (2001). Identification of a Carboxyl-terminal Diaphanous-related Formin Homology Protein Autoregulatory Domain. J. Biol. Chem. 276: 2824-2830 [Abstract] [Full Text]  
• Sharpless, K. E., Harris, S. D. (2002). Functional Characterization and Localization of the Aspergillus nidulans Formin SEPA. Mol. Biol. Cell 13: 469-479 [Abstract] [Full Text]  
• Wang, H., Tang, X., Liu, J., Trautmann, S., Balasundaram, D., McCollum, D., Balasubramanian, M. K. (2002). The Multiprotein Exocyst Complex Is Essential for Cell Separation in Schizosaccharomyces pombe. Mol. Biol. Cell 13: 515-529 [Abstract] [Full Text]  

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