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© The Rockefeller University Press, 0021-9525/1997//335 $5.00
The Journal of Cell Biology, Volume 137, Number 2, , 1997 335-345

Article

Mannose 6-Phosphate Receptors Regulate the Formation of Clathrin-coated Vesicles in the TGN



Roland Le Borgne and Bernard Hoflack

European Molecular Biology Laboratory, 69012 Heidelberg, Germany

The transport of the two mannose 6-phosphate receptors (MPRs) from the secretory pathway to the endocytic pathway is mediated by carrier vesicles coated with the AP-1 Golgi-specific assembly protein and clathrin. Using an in vitro assay that reconstitutes the ARF-1–dependent translocation of cytosolic AP-1 onto membranes of the TGN, we have previously reported that the MPRs are key components for the efficient recruitment of AP-1 (Le Borgne, R., G. Griffiths, and B. Hoflack. 1996. J. Biol. Chem. 271:2162–2170). Using a polyclonal antibody against the mouse {gamma}-adaptin, we have now examined the steady state distribution of AP-1 after subcellular fractionation of mouse fibroblasts lacking both MPRs or reexpressing physiological levels of either MPR. We report that the amount of AP-1 bound to membranes and associated with clathrin-coated vesicles depends on the expression level of the MPRs and on the integrity of their cytoplasmic domains. Thus, these results indicate that the concentration of the MPRs, i.e., the major transmembrane proteins sorted toward the endosomes, determines the number of clathrin-coated vesicles formed in the TGN.

1. Abbreviations used in this paper: CD, cation dependent; CI, cation independent; MPR, mannose 6-phosphate receptor; mTF, mouse Apo-transferrin; PI, phosphatidylinositol.

This research was supported in part by grants from the European Communities (Bio2-CT93-02205 and HCM ERB-CHRTXCT-940592) and the association "Vaincre les Maladies Lysosomales."

Please address all correspondence to Bernard Hoflack, Institut de Biologie de Lille, CNRS IFR 3, EP 525, Institut Pasteur de Lille, 1 rue Professeur Calmette, BP 447, 59021 Lille Cedex, France. Fax: (33) 3-20-87-10-19. e-mail: Bernard.Hoflack{at}Pasteur-Lille.fr


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