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© The Rockefeller University Press, 0021-9525/1997//377 $5.00
The Journal of Cell Biology, Volume 137, Number 2, , 1997 377-386

Article

Tim23, a Protein Import Component of the Mitochondrial Inner Membrane, Is Required for Normal Activity of the Multiple Conductance Channel, MCC



Timothy A. Lohret*, Robert E. Jensen{ddagger}, and Kathleen W. Kinnally*,§

* Department of Biological Sciences, University at Albany, SUNY, Albany, New York 12222; {ddagger} Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205; and § Department of Molecular Medicine, Wadsworth Center, NYS Department of Health, Empire State Plaza, Albany, New York 12201-0509

We previously showed that the conductance of a mitochondrial inner membrane channel, called MCC, was specifically blocked by peptides corresponding to mitochondrial import signals. To determine if MCC plays a role in protein import, we examined the relationship between MCC and Tim23p, a component of the protein import complex of the mitochondrial inner membrane. We find that antibodies against Tim23p, previously shown to inhibit mitochondrial protein import, inhibit MCC activity. We also find that MCC activity is altered in mitochondria isolated from yeast carrying the tim23-1 mutation. In contrast to wild-type MCC, we find that the conductance of MCC from the tim23-1 mutant is not significantly blocked by mitochondrial presequence peptides. Tim23 antibodies and the tim23-1 mutation do not, however, alter the activity of PSC, a presequence-peptide sensitive channel in the mitochondrial outer membrane. Our results show that Tim23p is required for normal MCC activity and raise the possibility that precursors are translocated across the inner membrane through the pore of MCC.

1. Abbreviations used in this paper: MCC, multiple conductance channel; PSC, peptide-sensitive channel; VDAC, voltage-dependent anion-selective channel.

This research was supported by National Science Foundation grant MCB9513439 (K.W. Kinnally) and U.S. Public Health Service grant RO1GM46803 (R.E. Jensen).

Please address all correspondence to Kathleen Kinnally, Wadsworth Center, Empire State Plaza, P.O. Box 509, Albany, NY 12201-0509. Tel.: (518) 474-4229. Fax: (518) 474-7992. E-mail: kinnally{at}wadsworth.org


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