Transport of a Large Oligomeric Protein by the Cytoplasm to Vacuole Protein Targeting Pathway

doi:10.1083/jcb.137.3.609

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© The Rockefeller University Press, 0021-9525/1997//609 $5.00
The Journal of Cell Biology, Volume 137, Number 3, , 1997 609-618

Article

Transport of a Large Oligomeric Protein by the Cytoplasm to Vacuole Protein Targeting Pathway

John Kim, Sidney V. Scott, Michael N. Oda, and Daniel J. Klionsky

Section of Microbiology, University of California, Davis, California 95616

Aminopeptidase I (API) is transported into the yeast vacuoleby the cytoplasm to vacuole targeting (Cvt) pathway. Geneticevidence suggests that autophagy, a major degradative pathwayin eukaryotes, and the Cvt pathway share largely the same cellularmachinery. To understand the mechanism of the Cvt import process,we examined the native state of API. Dodecameric assembly ofprecursor API in the cytoplasm and membrane binding were rapidevents, whereas subsequent vacuolar import appeared to be rate limiting. A unique temperature-sensitive API-targetingmutant allowed us to kinetically monitor its oligomeric stateduring translocation. Our findings indicate that API is maintainedas a dodecamer throughout its import and will be useful tostudy the posttranslational movement of folded proteins acrossbiological membranes.

1. Abbreviations used in this paper: ALP, alkaline phosphatase;API, aminopeptidase I; CPY, carboxypeptidase Y; Cvt, cytoplasmto vacuole targeting; ECF, enhanced chemifluorescence; PGK,phosphoglycerate kinase; PrA and PrB, proteinase A and B; SMD,synthetic minimal medium containing 2% glucose, essential aminoacids and ammonium sulfate.

Address all correspondence to Daniel J. Klionsky, Section ofMicrobiology, University of California, Davis, CA 95616. Tel.:(916) 752-0277. Fax: (916) 752-9014.

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